公开(公告)号：US07393631B2

公开(公告)日：2008-07-01

申请号：US11326275

申请日：2006-01-05

Applicant: Susan V. Cannon-Carlson ,  Collette Cutler ,  Gary J. Vellekamp ,  Marcio Voloch

Inventor： Susan V. Cannon-Carlson ,  Collette Cutler ,  Gary J. Vellekamp ,  Marcio Voloch

IPC: C12Q1/70

CPC classification number: C12N7/00 ,  C07K14/4746 ,  C12N2710/10351

Abstract: Methods for purifying adenoviruses from a contaminated sample using a hydroxyapatite medium are provided. Sodium chloride concentrations present in buffers used throughout the method are at least 150 mM to prevent the adenovirus from irreversibly binding to the hydroxyapatite. Levels of contaminants and empty adenovirus capsids from samples purified by conventional purification techniques are further reduced to provide a highly pure adenovirus preparation.

Abstract translation: 提供了使用羟基磷灰石介质从污染样品中纯化腺病毒的方法。 存在于整个方法中使用的缓冲液中的氯化钠浓度为至少150mM，以防止腺病毒与羟磷灰石不可逆地结合。 来自通过常规纯化技术纯化的样品的污染物和空的腺病毒衣壳的水平进一步降低以提供高纯度的腺病毒制剂。

公开(公告)号：US20080293123A1

公开(公告)日：2008-11-27

申请号：US12188067

申请日：2008-08-07

Applicant: Andreas Frei ,  Henry K.H. Kwan ,  Varda E. Sandweiss ,  Gary J. Vellekamp ,  Pui-Ho Yuen ,  Laureano L. Bondoc, JR. ,  Frederick William Porter, IV ,  John Chu-Tay Tang ,  Peter Ihnat

Inventor： Andreas Frei ,  Henry K.H. Kwan ,  Varda E. Sandweiss ,  Gary J. Vellekamp ,  Pui-Ho Yuen ,  Laureano L. Bondoc, JR. ,  Frederick William Porter, IV ,  John Chu-Tay Tang ,  Peter Ihnat

IPC: C12N7/02

CPC classification number: C12N7/00 ,  A61K39/12 ,  A61K39/235 ,  A61K48/0091 ,  C12N15/86 ,  C12N2710/10334 ,  C12N2710/10343 ,  C12N2710/10351 ,  Y10S514/937

Abstract: A composition is disclosed comprising virus in a formulation comprising a polyhydroxy hydrocarbon buffered to maintain a pH in a range from about 7 to about 8.5 at a temperature in the range from about 2° C. to 27° C. Methods for concentrating and purifying virus preparations are also disclosed.

Abstract translation: 公开了一种组合物，其包含缓冲的多羟基烃的制剂中的病毒，以在约2℃至27℃的温度范围内将pH保持在约7至约8.5的范围内。浓缩和纯化病毒的方法 准备工作也被披露。

公开(公告)号：US06544769B1

公开(公告)日：2003-04-08

申请号：US09249646

申请日：1999-02-12

Applicant: Andreas Frei ,  Henry K. H. Kwan ,  Varda E. Sandweiss ,  Gary J. Vellekamp ,  Pui-Ho Yuen ,  Peter Ihnat

Inventor： Andreas Frei ,  Henry K. H. Kwan ,  Varda E. Sandweiss ,  Gary J. Vellekamp ,  Pui-Ho Yuen ,  Peter Ihnat

IPC: C12N700

CPC classification number: C12N7/00 ,  A61K39/12 ,  A61K39/235 ,  A61K48/0091 ,  C12N15/86 ,  C12N2710/10334 ,  C12N2710/10343 ,  C12N2710/10351 ,  Y10S514/937

Abstract: A composition is disclosed comprising virus in a formulation comprising a polyhydroxy hydrocarbon buffered to maintain a pH in a range from about 7 to about 8.5 at a temperature in the range from about 2° C. to 27° C. Methods for concentrating and purifying virus preparations are also disclosed.

Abstract translation: 公开了一种组合物，其包含缓冲的多羟基烃的制剂中的病毒，以在约2℃至27℃的温度范围内将pH保持在约7至约8.5的范围内。浓缩和纯化病毒的方法 准备工作也被披露。

公开(公告)号：US20090098632A1

公开(公告)日：2009-04-16

申请号：US12080653

申请日：2008-04-04

Applicant: Andreas Frei ,  Henry K.H. Kwan ,  Varda E. Sandweiss ,  Gary J. Vellekamp ,  Pui-Ho Yuen ,  Laureano L. Bondoc, JR. ,  Frederick William Porter, IV ,  John Chu-Tay Tang ,  Peter Ihnat

Inventor： Andreas Frei ,  Henry K.H. Kwan ,  Varda E. Sandweiss ,  Gary J. Vellekamp ,  Pui-Ho Yuen ,  Laureano L. Bondoc, JR. ,  Frederick William Porter, IV ,  John Chu-Tay Tang ,  Peter Ihnat

IPC: C12N7/00

CPC classification number: C12N7/00 ,  A61K39/12 ,  A61K39/235 ,  A61K48/0091 ,  C12N15/86 ,  C12N2710/10334 ,  C12N2710/10343 ,  C12N2710/10351 ,  Y10S514/937

Abstract: A composition is disclosed comprising virus in a formulation comprising a polyhydroxy hydrocarbon buffered to maintain a pH in a range from about 7 to about 8.5 at a temperature in the range from about 2° C. to 27° C. Methods for concentrating and purifying virus preparations are also disclosed.

Abstract translation: 公开了一种组合物，其包含缓冲的多羟基烃的制剂中的病毒，以在约2℃至27℃的温度范围内将pH保持在约7至约8.5的范围内。浓缩和纯化病毒的方法 准备工作也被披露。

公开(公告)号：US20080194002A1

公开(公告)日：2008-08-14

申请号：US12082853

申请日：2008-04-14

Applicant: Andreas Frei ,  Henry K.H. Kwan ,  Varda E. Sandweiss ,  Gary J. Vellekamp ,  Pui-Ho Yuen ,  Laureano L. Bondoc ,  Frederick William Porter ,  John Chu-Tay Tang ,  Peter Ihnat

Inventor： Andreas Frei ,  Henry K.H. Kwan ,  Varda E. Sandweiss ,  Gary J. Vellekamp ,  Pui-Ho Yuen ,  Laureano L. Bondoc ,  Frederick William Porter ,  John Chu-Tay Tang ,  Peter Ihnat

IPC: C12N7/02

CPC classification number: C12N7/00 ,  A61K39/12 ,  A61K39/235 ,  A61K48/0091 ,  C12N15/86 ,  C12N2710/10334 ,  C12N2710/10343 ,  C12N2710/10351 ,  Y10S514/937

Abstract: A composition is disclosed comprising virus in a formulation comprising a polyhydroxy hydrocarbon buffered to maintain a pH in a range from about 7 to about 8.5 at a temperature in the range from about 2° C. to 27° C. Methods for concentrating and purifying virus preparations are also disclosed.

Abstract translation: 公开了一种组合物，其包含缓冲的多羟基烃的制剂中的病毒，以在约2℃至27℃的温度范围内将pH保持在约7至约8.5的范围内。浓缩和纯化病毒的方法 准备工作也被披露。

公开(公告)号：US20120093777A1

公开(公告)日：2012-04-19

申请号：US13334403

申请日：2011-12-22

Applicant: Andreas Frei ,  Henry K.H. Kwan ,  Varda E. Sandweiss ,  Gary J. Vellekamp ,  Pui-Ho Yuen ,  Laureano L. Bondoc, JR. ,  Frederick William Porter, IV ,  John Chu-Tay Tang ,  Peter Ihnat

Inventor： Andreas Frei ,  Henry K.H. Kwan ,  Varda E. Sandweiss ,  Gary J. Vellekamp ,  Pui-Ho Yuen ,  Laureano L. Bondoc, JR. ,  Frederick William Porter, IV ,  John Chu-Tay Tang ,  Peter Ihnat

IPC: A61K35/76

CPC classification number: C12N7/00 ,  A61K39/12 ,  A61K39/235 ,  A61K48/0091 ,  C12N15/86 ,  C12N2710/10334 ,  C12N2710/10343 ,  C12N2710/10351 ,  Y10S514/937

Abstract: A composition is disclosed comprising virus in a formulation comprising a polyhydroxy hydrocarbon buffered to maintain a pH in a range from about 7 to about 8.5 at a temperature in the range from about 2° C. to 27° C. Methods for concentrating and purifying virus preparations are also disclosed.

Abstract translation: 公开了一种组合物，其包含缓冲的多羟基烃的制剂中的病毒，以在约2℃至27℃的温度范围内将pH保持在约7至约8.5的范围内。浓缩和纯化病毒的方法 准备工作也被披露。

公开(公告)号：US20110257368A1

公开(公告)日：2011-10-20

申请号：US13141269

申请日：2009-12-17

Applicant: Xiaoyu Yang ,  Gary J. Vellekamp

Inventor： Xiaoyu Yang ,  Gary J. Vellekamp

IPC: C07K1/18

CPC classification number: C07K14/56

Abstract: The present invention provides a method for separating desired interferon isoforms from undesired interferon isoforms that involves subjecting the isoforms to anion exchange column chromatography and a biphasic elution procedure. A strong elution solution is used in the first elution phase to facilitate elution of the desired isoform from the column and a weak elution solution is used in the second phase to suppress elution of the desired isoforms.

Abstract translation: 本发明提供了从期望的干扰素同种型分离所需干扰素同种型的方法，其包括使同种型进行阴离子交换柱色谱和双相洗脱程序。 在第一洗脱阶段使用强洗脱溶液以便于从柱中洗脱所需的同种型，并且在第二阶段使用弱洗脱溶液以抑制所需同种型的洗脱。

公开(公告)号：US20080138318A1

公开(公告)日：2008-06-12

申请号：US11932302

申请日：2007-10-31

Applicant: Andreas Frei ,  Henry K.H. Kwan ,  Varda E. Sandweiss ,  Gary J. Vellekamp ,  Pui-Ho Yuen ,  Laureano L. Bondoc ,  Frederick William Porter ,  John Chu-Tay Tang ,  Peter Ihnat

Inventor： Andreas Frei ,  Henry K.H. Kwan ,  Varda E. Sandweiss ,  Gary J. Vellekamp ,  Pui-Ho Yuen ,  Laureano L. Bondoc ,  Frederick William Porter ,  John Chu-Tay Tang ,  Peter Ihnat

IPC: A61K35/76

CPC classification number: C12N7/00 ,  A61K39/12 ,  A61K39/235 ,  A61K48/0091 ,  C12N15/86 ,  C12N2710/10334 ,  C12N2710/10343 ,  C12N2710/10351 ,  Y10S514/937

Abstract: A composition is disclosed comprising virus in a formulation comprising a polyhydroxy hydrocarbon buffered to maintain a pH in a range from about 7 to about 8.5 at a temperature in the range from about 20° C. to 27° C. Methods for concentrating and purifying virus preparations are also disclosed.

Abstract translation: 公开了一种组合物，该组合物包含缓冲的多羟基烃的制剂中的病毒，以在约20℃至27℃的温度范围内将pH保持在约7至约8.5的范围内。浓缩和纯化病毒的方法 准备工作也被披露。