公开(公告)号：US07393631B2

公开(公告)日：2008-07-01

申请号：US11326275

申请日：2006-01-05

Applicant: Susan V. Cannon-Carlson ,  Collette Cutler ,  Gary J. Vellekamp ,  Marcio Voloch

Inventor： Susan V. Cannon-Carlson ,  Collette Cutler ,  Gary J. Vellekamp ,  Marcio Voloch

IPC: C12Q1/70

CPC classification number: C12N7/00 ,  C07K14/4746 ,  C12N2710/10351

Abstract: Methods for purifying adenoviruses from a contaminated sample using a hydroxyapatite medium are provided. Sodium chloride concentrations present in buffers used throughout the method are at least 150 mM to prevent the adenovirus from irreversibly binding to the hydroxyapatite. Levels of contaminants and empty adenovirus capsids from samples purified by conventional purification techniques are further reduced to provide a highly pure adenovirus preparation.

Abstract translation: 提供了使用羟基磷灰石介质从污染样品中纯化腺病毒的方法。 存在于整个方法中使用的缓冲液中的氯化钠浓度为至少150mM，以防止腺病毒与羟磷灰石不可逆地结合。 来自通过常规纯化技术纯化的样品的污染物和空的腺病毒衣壳的水平进一步降低以提供高纯度的腺病毒制剂。

公开(公告)号：US20060105456A1

公开(公告)日：2006-05-18

申请号：US11326275

申请日：2006-01-05

Applicant: Susan Cannon-Carlson ,  Collette Cutler ,  Gary Vellekamp ,  Marcio Voloch

Inventor： Susan Cannon-Carlson ,  Collette Cutler ,  Gary Vellekamp ,  Marcio Voloch

IPC: C12Q1/68 ,  C12N5/00 ,  C12N5/02

CPC classification number: C12N7/00 ,  C07K14/4746 ,  C12N2710/10351

Abstract: Methods for purifying adenoviruses from a contaminated sample using a hydroxyapatite medium are provided. Sodium chloride concentrations present in buffers used throughout the method are at least 150 mM to prevent the adenovirus from irreversibly binding to the hydroxyapatite. Levels of contaminants and empty adenovirus capsids from samples purified by conventional purification techniques are further reduced to provide a highly pure adenovirus preparation.