公开(公告)号：US20090098632A1

公开(公告)日：2009-04-16

申请号：US12080653

申请日：2008-04-04

Applicant: Andreas Frei ,  Henry K.H. Kwan ,  Varda E. Sandweiss ,  Gary J. Vellekamp ,  Pui-Ho Yuen ,  Laureano L. Bondoc, JR. ,  Frederick William Porter, IV ,  John Chu-Tay Tang ,  Peter Ihnat

Inventor： Andreas Frei ,  Henry K.H. Kwan ,  Varda E. Sandweiss ,  Gary J. Vellekamp ,  Pui-Ho Yuen ,  Laureano L. Bondoc, JR. ,  Frederick William Porter, IV ,  John Chu-Tay Tang ,  Peter Ihnat

IPC: C12N7/00

CPC classification number: C12N7/00 ,  A61K39/12 ,  A61K39/235 ,  A61K48/0091 ,  C12N15/86 ,  C12N2710/10334 ,  C12N2710/10343 ,  C12N2710/10351 ,  Y10S514/937

Abstract: A composition is disclosed comprising virus in a formulation comprising a polyhydroxy hydrocarbon buffered to maintain a pH in a range from about 7 to about 8.5 at a temperature in the range from about 2° C. to 27° C. Methods for concentrating and purifying virus preparations are also disclosed.

Abstract translation: 公开了一种组合物，其包含缓冲的多羟基烃的制剂中的病毒，以在约2℃至27℃的温度范围内将pH保持在约7至约8.5的范围内。浓缩和纯化病毒的方法 准备工作也被披露。

公开(公告)号：US20080194002A1

公开(公告)日：2008-08-14

申请号：US12082853

申请日：2008-04-14

Applicant: Andreas Frei ,  Henry K.H. Kwan ,  Varda E. Sandweiss ,  Gary J. Vellekamp ,  Pui-Ho Yuen ,  Laureano L. Bondoc ,  Frederick William Porter ,  John Chu-Tay Tang ,  Peter Ihnat

Inventor： Andreas Frei ,  Henry K.H. Kwan ,  Varda E. Sandweiss ,  Gary J. Vellekamp ,  Pui-Ho Yuen ,  Laureano L. Bondoc ,  Frederick William Porter ,  John Chu-Tay Tang ,  Peter Ihnat

IPC: C12N7/02

CPC classification number: C12N7/00 ,  A61K39/12 ,  A61K39/235 ,  A61K48/0091 ,  C12N15/86 ,  C12N2710/10334 ,  C12N2710/10343 ,  C12N2710/10351 ,  Y10S514/937

Abstract: A composition is disclosed comprising virus in a formulation comprising a polyhydroxy hydrocarbon buffered to maintain a pH in a range from about 7 to about 8.5 at a temperature in the range from about 2° C. to 27° C. Methods for concentrating and purifying virus preparations are also disclosed.

Abstract translation: 公开了一种组合物，其包含缓冲的多羟基烃的制剂中的病毒，以在约2℃至27℃的温度范围内将pH保持在约7至约8.5的范围内。浓缩和纯化病毒的方法 准备工作也被披露。

公开(公告)号：US07390652B2

公开(公告)日：2008-06-24

申请号：US10919086

申请日：2004-08-16

Applicant: Russell G. G. Condon ,  Nancy V. Connelly ,  Andreas Frei ,  Edward Glowacki ,  Vijay Yabannavar ,  Serge Batandolo

Inventor： Russell G. G. Condon ,  Nancy V. Connelly ,  Andreas Frei ,  Edward Glowacki ,  Vijay Yabannavar ,  Serge Batandolo

IPC: C12M1/00 ,  C12M1/40

CPC classification number: C12N15/86 ,  C12N5/0075 ,  C12N7/00 ,  C12N2531/00 ,  C12N2710/10321 ,  C12N2710/10343 ,  C12N2710/10351

Abstract: This invention relates to methods for the cultivating cells, and in particular to methods for propagating viruses.

Abstract translation: 本发明涉及培养细胞的方法，特别涉及传播病毒的方法。

公开(公告)号：US20060018930A1

公开(公告)日：2006-01-26

申请号：US11053181

申请日：2005-02-07

Applicant: Andreas Frei ,  Henry Kwan ,  Varda Sandweiss ,  Gary Vellekamp ,  Pui-Ho Yuen ,  Laureano Bondoc ,  Frederick Porter ,  John Tang ,  Peter Ihnat

Inventor： Andreas Frei ,  Henry Kwan ,  Varda Sandweiss ,  Gary Vellekamp ,  Pui-Ho Yuen ,  Laureano Bondoc ,  Frederick Porter ,  John Tang ,  Peter Ihnat

IPC: A61K39/23 ,  C12N7/00 ,  A61K31/7012

CPC classification number: C12N7/00 ,  A61K39/12 ,  A61K39/235 ,  A61K48/0091 ,  C12N15/86 ,  C12N2710/10334 ,  C12N2710/10343 ,  C12N2710/10351 ,  Y10S514/937

Abstract: A composition is disclosed comprising virus in a formulation comprising a polyhydroxy hydrocarbon buffered to maintain a pH in a range from about 7 to about 8.5 at a temperature in the range from about 2° C. to 27° C. Methods for concentrating and purifying virus preparations are also disclosed.

公开(公告)号：US20050019913A1

公开(公告)日：2005-01-27

申请号：US10919086

申请日：2004-08-16

Applicant: Russell Condon ,  Nancy Connelly ,  Andreas Frei ,  Edward Glowacki ,  Vijay Yabannavar ,  Serge Batandolo

Inventor： Russell Condon ,  Nancy Connelly ,  Andreas Frei ,  Edward Glowacki ,  Vijay Yabannavar ,  Serge Batandolo

IPC: C12N5/00 ,  C12N7/00 ,  C12N15/861

CPC classification number: C12N15/86 ,  C12N5/0075 ,  C12N7/00 ,  C12N2531/00 ,  C12N2710/10321 ,  C12N2710/10343 ,  C12N2710/10351

Abstract: This invention relates to methods for the cultivating cells, and in particular to methods for propagating viruses.

Abstract translation: 本发明涉及培养细胞的方法，特别涉及传播病毒的方法。

公开(公告)号：US06168944A

公开(公告)日：2001-01-02

申请号：US09020572

申请日：1998-01-28

Applicant: Russell G. G. Condon ,  Nancy V. Connelly ,  Andreas Frei ,  Edward Glowacki ,  Vijay Yabannavar ,  Serge Batandolo

Inventor： Russell G. G. Condon ,  Nancy V. Connelly ,  Andreas Frei ,  Edward Glowacki ,  Vijay Yabannavar ,  Serge Batandolo

IPC: C12N702

CPC classification number: C12N7/00 ,  C12M23/02 ,  C12N2710/10351

Abstract: This invention relates to methods for the cultivating cells, and in particular to methods for propagating recombinant viruses for gene therapy.

Abstract translation: 本发明涉及培养细胞的方法，特别涉及用于繁殖用于基因治疗的重组病毒的方法。

公开(公告)号：US20120093777A1

公开(公告)日：2012-04-19

申请号：US13334403

申请日：2011-12-22

Applicant: Andreas Frei ,  Henry K.H. Kwan ,  Varda E. Sandweiss ,  Gary J. Vellekamp ,  Pui-Ho Yuen ,  Laureano L. Bondoc, JR. ,  Frederick William Porter, IV ,  John Chu-Tay Tang ,  Peter Ihnat

Inventor： Andreas Frei ,  Henry K.H. Kwan ,  Varda E. Sandweiss ,  Gary J. Vellekamp ,  Pui-Ho Yuen ,  Laureano L. Bondoc, JR. ,  Frederick William Porter, IV ,  John Chu-Tay Tang ,  Peter Ihnat

IPC: A61K35/76

CPC classification number: C12N7/00 ,  A61K39/12 ,  A61K39/235 ,  A61K48/0091 ,  C12N15/86 ,  C12N2710/10334 ,  C12N2710/10343 ,  C12N2710/10351 ,  Y10S514/937

Abstract: A composition is disclosed comprising virus in a formulation comprising a polyhydroxy hydrocarbon buffered to maintain a pH in a range from about 7 to about 8.5 at a temperature in the range from about 2° C. to 27° C. Methods for concentrating and purifying virus preparations are also disclosed.

Abstract translation: 公开了一种组合物，其包含缓冲的多羟基烃的制剂中的病毒，以在约2℃至27℃的温度范围内将pH保持在约7至约8.5的范围内。浓缩和纯化病毒的方法 准备工作也被披露。

公开(公告)号：US20080261289A1

公开(公告)日：2008-10-23

申请号：US12147391

申请日：2008-06-26

Applicant: Andreas Frei ,  Henry K.H. Kwan ,  Varda E. Sandweiss ,  Gary J. Vellkamp ,  Pui-Ho Yuen ,  Laureano L. Bondoc ,  Frederick William Porter ,  John Chu-Tay Tang ,  Peter Ihnat

Inventor： Andreas Frei ,  Henry K.H. Kwan ,  Varda E. Sandweiss ,  Gary J. Vellkamp ,  Pui-Ho Yuen ,  Laureano L. Bondoc ,  Frederick William Porter ,  John Chu-Tay Tang ,  Peter Ihnat

IPC: C12N7/00

CPC classification number: C12N15/86 ,  C12N7/00 ,  C12N2710/10343 ,  C12N2710/10351

Abstract: A composition is disclosed comprising virus in a formulation comprising a polyhydroxy hydrocarbon buffered to maintain a pH in a range from about 7 to about 8.5 at a temperature in the range from about 2° C. to 27° C. Methods for concentrating and purifying virus preparations are also disclosed.

公开(公告)号：US20080138318A1

公开(公告)日：2008-06-12

申请号：US11932302

申请日：2007-10-31

Applicant: Andreas Frei ,  Henry K.H. Kwan ,  Varda E. Sandweiss ,  Gary J. Vellekamp ,  Pui-Ho Yuen ,  Laureano L. Bondoc ,  Frederick William Porter ,  John Chu-Tay Tang ,  Peter Ihnat

Inventor： Andreas Frei ,  Henry K.H. Kwan ,  Varda E. Sandweiss ,  Gary J. Vellekamp ,  Pui-Ho Yuen ,  Laureano L. Bondoc ,  Frederick William Porter ,  John Chu-Tay Tang ,  Peter Ihnat

IPC: A61K35/76

CPC classification number: C12N7/00 ,  A61K39/12 ,  A61K39/235 ,  A61K48/0091 ,  C12N15/86 ,  C12N2710/10334 ,  C12N2710/10343 ,  C12N2710/10351 ,  Y10S514/937

Abstract: A composition is disclosed comprising virus in a formulation comprising a polyhydroxy hydrocarbon buffered to maintain a pH in a range from about 7 to about 8.5 at a temperature in the range from about 20° C. to 27° C. Methods for concentrating and purifying virus preparations are also disclosed.

Abstract translation: 公开了一种组合物，该组合物包含缓冲的多羟基烃的制剂中的病毒，以在约20℃至27℃的温度范围内将pH保持在约7至约8.5的范围内。浓缩和纯化病毒的方法 准备工作也被披露。

公开(公告)号：US20140011212A1

公开(公告)日：2014-01-09

申请号：US13982596

申请日：2012-01-30

Applicant: Andreas Frei ,  Bernd Wollscheid ,  Ock-Youm Jeon ,  Erick Carreira

Inventor： Andreas Frei ,  Bernd Wollscheid ,  Ock-Youm Jeon ,  Erick Carreira

IPC: G01N33/566

CPC classification number: G01N33/566 ,  C07D495/04 ,  G01N33/582 ,  G01N2333/70596 ,  Y02P20/55

Abstract: The present invention relates to trifunctional crosslinking reagents carrying (i) a ligand-reactive group for conjugation to a ligand of interest having at least one binding site on a target glycoprotein receptor, (ii) an optionally protected aromatic hydrazine group for the capturing of oxidized receptor-glycopeptides and (iii) an affinity group for the detection, isolation and purification of captured glycopeptides, their methods of production, as well as their use in methods for detecting, identifying and characterizing interactions between ligands and their corresponding glycoprotein target receptors on living cells and in biological fluids.

Abstract translation: 本发明涉及三官能交联剂，其携带（i）用于与靶糖配位体上至少有一个结合位点缀合的目的配体的配体 - 反应性基团，（ii）任选保护的芳族肼基团，用于捕获氧化的 受体 - 糖肽和（iii）用于检测，分离和纯化捕获的糖肽，其生产方法的亲和基团，以及它们用于检测，鉴定和表征配体与其相应的生物体内糖蛋白靶受体之间的相互作用的方法 细胞和生物液体。