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1.发明授权Monoclonal antibodies and detection methods for enzymes that confer resistance to 2,4-dichlorophenoxyacetic acid 有权单克隆抗体和对2,4-二氯苯氧基乙酸具有抗性的酶的检测方法公开(公告)号：US08673583B2
公开(公告)日：2014-03-18
申请号：US13218498
申请日：2011-08-26
Applicant: Shawna K. Embrey , Gaofeng Lin , Guomin Shan
Inventor： Shawna K. Embrey , Gaofeng Lin , Guomin Shan
IPC: G01N33/573 , G01N33/543 , C12Q1/26 , C07K16/40 , C07K16/12
CPC classification number: C07K16/1203 , C07K16/40 , C12Q1/26 , G01N33/543 , G01N33/573 , G01N2333/195 , G01N2333/90 , G01N2333/90241
Abstract: Described herein are monoclonal antibodies and methods useful for determining and quantitating the presence of AAD-1 (aryloxyalkanoate dioxygenase) enzyme. These monoclonal antibodies are surprisingly well suited for detecting AAD-1 transgenic event gene products in a variety of plants and plant tissues. The invention further provides quantitative and qualitative immunoassays using the immunoglobulins of the invention.
Abstract translation: 本文描述的是用于测定和定量AAD-1（芳氧基链烷酸酯加氧酶）酶的存在的单克隆抗体和方法。 这些单克隆抗体非常适合用于检测各种植物和植物组织中的AAD-1转基因事件基因产物。 本发明还提供了使用本发明的免疫球蛋白的定量和定性免疫测定。
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2.发明授权Monoclonal antibodies detection methods for enzymes that confer resistance to 2,4-dichlorophenoxyacetic acid in plants 有权在植物中赋予2,4-二氯苯氧基乙酸抗性的酶的单克隆抗体检测方法公开(公告)号：US08460891B2
公开(公告)日：2013-06-11
申请号：US13151389
申请日：2011-06-02
Applicant: Guomin Shan , Gaofeng Lin , Joelene K. Smith Drake , Marcelo Sosa
Inventor： Guomin Shan , Gaofeng Lin , Joelene K. Smith Drake , Marcelo Sosa
IPC: G01N33/573 , G01N33/53 , C12N5/12 , C07K16/16
CPC classification number: G01N33/573 , C07K16/40
Abstract: Described herein are monoclonal antibodies and methods useful for determining and quantitating the presence of an aryloxyalkanoate dioxygenase enzyme.
Abstract translation: 本文描述的是用于测定和定量芳氧基链烷酸酯双加氧酶的存在的单克隆抗体和方法。
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3.发明申请公开(公告)号：US20120244569A1
公开(公告)日：2012-09-27
申请号：US13427750
申请日：2012-03-22
Applicant: Jayakumar P. SAMUEL , Narasimha C. SAMBOJU , Kerrm Y. YAU , Gaofeng LIN , Steven R. WEBB , Frank BURROUGHS
Inventor： Jayakumar P. SAMUEL , Narasimha C. SAMBOJU , Kerrm Y. YAU , Gaofeng LIN , Steven R. WEBB , Frank BURROUGHS
CPC classification number: C12N15/8206 , B82Y5/00 , C12N15/8207 , C12N15/8221
Abstract: Provided are methods for introducing a molecule of interest into a plant cell having a cell wall by using a QD-peptide conjugate having a quantum dot (QD) with one or more cell penetrating peptides (CPPs). Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.
Abstract translation: 提供了通过使用具有一个或多个细胞穿透肽（CPP）的具有量子点（QD）的QD-肽缀合物将感兴趣的分子引入具有细胞壁的植物细胞的方法。 提供用于遗传或其他修饰植物和用于治疗或预防包含细胞壁的植物细胞中的疾病的方法。
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公开(公告)号：US20110203017A1
公开(公告)日：2011-08-18
申请号：US12091896
申请日：2006-10-27
Applicant: Terry R. Wright , Justin M. Lira , Terence Anthony Walsh , Donald J. Merlo , Jayakumar P. Samuel , Gaofeng Lin
Inventor： Terry R. Wright , Justin M. Lira , Terence Anthony Walsh , Donald J. Merlo , Jayakumar P. Samuel , Gaofeng Lin
CPC classification number: C12N15/8274 , C12N9/0071 , C12N15/8275
Abstract: The subject invention provides novel plants that are not only resistant to 2,4-D, but also to pyridyloxyacetate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention “stacked” together with one or more other herbicide resistance genes. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the development of, and controlling, strains of weeds that are resistant to one or more herbicides such as glyphosate. The preferred enzyme and gene for use according to the subject invention are referred to herein as AAD-12 (AryloxyAlkanoate Dioxygenase). This highly novel discovery is the basis of significant herbicide tolerant crop trait and selectable marker opportunities.
Abstract translation: 本发明提供了不仅对2,4-D有抗性的新型植物，而且还提供了对吡啶氧基乙酸酯除草剂的抗性。 迄今为止，没有任何期望或建议，通过引入单一基因可以产生具有这两种有利性质的植物。 本发明还包括与一种或多种其它除草剂抗性基因一起产生本发明一种或多种酶“堆叠”的植物。 本发明能够以新的方式使用除草剂的新型组合。 此外，本发明提供了防止对一种或多种除草剂如草甘膦具有抗性的杂草的发育和控制菌株的新方法。 根据本发明使用的优选酶和基因在本文中称为AAD-12（丙烯酰氧基烷酸酯加氧酶）。 这种高度新颖的发现是显着的除草剂耐受性作物性状和选择性标记机会的基础。
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5.发明授权公开(公告)号：US08581036B2
公开(公告)日：2013-11-12
申请号：US13042565
申请日：2011-03-08
Applicant: Narasimha C. Samboju , Jayakumar P. Samuel , Gaofeng Lin , Steven R. Webb , Frank Burroughs
Inventor： Narasimha C. Samboju , Jayakumar P. Samuel , Gaofeng Lin , Steven R. Webb , Frank Burroughs
CPC classification number: C12N15/8206
Abstract: A method of introducing a molecule of interest into a plant cell having a cell wall includes interacting a gamma-zein peptide with a molecule of interest to form a gamma-zein linked structure. The gamma-zein linked structure is then placed in contact with the plant cell having a cell wall, and allowing uptake of the gamma-zein linked structure into the plant cell. Alternatively, a gene of interest can be expressed in a plant cell having an intact cell wall by interacting a gamma-zein peptide with the gene of interest to form a gamma-zein linked gene structure, allowing uptake of the gamma-zein linked gene structure into the plant cell, and expressing the gene of interest in the plant cell and its progeny.
Abstract translation: 将感兴趣的分子引入具有细胞壁的植物细胞中的方法包括将γ-玉米醇溶蛋白肽与感兴趣的分子相互作用以形成γ-玉米醇溶蛋白连接的结构。 然后将γ-玉米醇溶蛋白连接的结构与具有细胞壁的植物细胞接触，并允许将γ-玉米醇溶蛋白连接的结构摄取到植物细胞中。 或者，通过将γ-玉米醇溶蛋白肽与感兴趣的基因相互作用以形成γ-玉米醇溶蛋白连接的基因结构，可以在具有完整细胞壁的植物细胞中表达感兴趣的基因，从而允许γ-玉米醇溶蛋白连接的基因结构 进入植物细胞，并在植物细胞及其后代中表达感兴趣的基因。
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Patent Agency Ranking
6.发明申请MONOCLONAL ANTIBODIES AND DETECTION METHODS FOR ENZYMES THAT CONFER RESISTANCE TO 2,4-DICHLOROPHENOXYACETIC ACID 有权单克隆抗体和检测方法，用于对2,4-二氯己氧酸进行耐受的酶公开(公告)号：US20120064542A1
公开(公告)日：2012-03-15
申请号：US13218498
申请日：2011-08-26
Applicant: Shawna K. EMBREY , Gaofeng LIN , Guomin SHAN
Inventor： Shawna K. EMBREY , Gaofeng LIN , Guomin SHAN
IPC: G01N33/577 , C12N5/16 , C07K16/40
CPC classification number: C07K16/1203 , C07K16/40 , C12Q1/26 , G01N33/543 , G01N33/573 , G01N2333/195 , G01N2333/90 , G01N2333/90241
Abstract: Described herein are monoclonal antibodies and methods useful for determining and quantitating the presence of AAD-1 (aryloxyalkanoate dioxygenase) enzyme. These monoclonal antibodies are surprisingly well suited for detecting AAD-1 transgenic event gene products in a variety of plants and plant tissues. The invention further provides quantitative and qualitative immunoassays using the immunoglobulins of the invention.
Abstract translation: 本文描述的是用于测定和定量AAD-1（芳氧基链烷酸酯加氧酶）酶的存在的单克隆抗体和方法。 这些单克隆抗体非常适合用于检测各种植物和植物组织中的AAD-1转基因事件基因产物。 本发明还提供了使用本发明的免疫球蛋白的定量和定性免疫测定。
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7.发明授权Quantum dot carrier peptide conjugates suitable for imaging and delivery applications in plants 有权量子点载体肽缀合物，适用于植物中的成像和递送应用公开(公告)号：US08609420B2
公开(公告)日：2013-12-17
申请号：US13427750
申请日：2012-03-22
Applicant: Jayakumar P. Samuel , Narasimha C. Samboju , Kerrm Y. Yau , Gaofeng Lin , Steven R. Webb , Frank Burroughs
Inventor： Jayakumar P. Samuel , Narasimha C. Samboju , Kerrm Y. Yau , Gaofeng Lin , Steven R. Webb , Frank Burroughs
CPC classification number: C12N15/8206 , B82Y5/00 , C12N15/8207 , C12N15/8221
Abstract: Provided are methods for introducing a molecule of interest into a plant cell having a cell wall by using a QD-peptide conjugate having a quantum dot (QD) with one or more cell penetrating peptides (CPPs). Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.
Abstract translation: 提供通过使用具有一个或多个细胞穿透肽（CPP）的具有量子点（QD）的QD-肽缀合物将感兴趣的分子引入具有细胞壁的植物细胞的方法。 提供用于遗传或其他修饰植物以及用于治疗或预防包含细胞壁的植物细胞中的疾病的方法。
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8.发明申请MONOCLONAL ANTIBODIES DETECTION METHODS FOR ENZYMES THAT CONFER RESISTANCE TO 2,4-DICHLOROPHENOXYACETIC ACID IN PLANTS 有权用于植物中2,4-二氯甲氧基酸的耐受性的单克隆抗体检测方法公开(公告)号：US20110300556A1
公开(公告)日：2011-12-08
申请号：US13151389
申请日：2011-06-02
Applicant: Guomin Shan , Gaofeng Lin , Joelene K. Smith Drake , Marcelo Sosa
Inventor： Guomin Shan , Gaofeng Lin , Joelene K. Smith Drake , Marcelo Sosa
IPC: G01N33/573 , C12N5/16 , C07K16/40
CPC classification number: G01N33/573 , C07K16/40
Abstract: Described herein are monoclonal antibodies and methods useful for determining and quantitating the presence of an aryloxyalkanoate dioxygenase enzyme.
Abstract translation: 本文描述的是用于测定和定量芳氧基链烷酸酯双加氧酶的存在的单克隆抗体和方法。
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9.发明申请PLANT PEPTIDE GAMMA-ZEIN FOR DELIVERY OF BIOMOLECULES INTO PLANT CELLS 有权用于将生物分子植入植物细胞的植物肽GAMMA-ZEIN公开(公告)号：US20110247100A1
公开(公告)日：2011-10-06
申请号：US13042565
申请日：2011-03-08
Applicant: Narasimha C. Samboju , Jayakumar P. Samuel , Gaofeng Lin , Steven R. Webb , Frank Burroughs
Inventor： Narasimha C. Samboju , Jayakumar P. Samuel , Gaofeng Lin , Steven R. Webb , Frank Burroughs
CPC classification number: C12N15/8206
Abstract: A method of introducing a molecule of interest into a plant cell having a cell wall includes interacting a gamma-zein peptide with a molecule of interest to form a gamma-zein linked structure. The gamma-zein linked structure is then placed in contact with the plant cell having a cell wall, and allowing uptake of the gamma-zein linked structure into the plant cell. Alternatively, a gene of interest can be expressed in a plant cell having an intact cell wall by interacting a gamma-zein peptide with the gene of interest to form a gamma-zein linked gene structure, allowing uptake of the gamma-zein linked gene structure into the plant cell, and expressing the gene of interest in the plant cell and its progeny.
Abstract translation: 将感兴趣的分子引入具有细胞壁的植物细胞中的方法包括将γ-玉米醇溶蛋白肽与感兴趣的分子相互作用以形成γ-玉米醇溶蛋白连接的结构。 然后将γ-玉米醇溶蛋白连接的结构与具有细胞壁的植物细胞接触，并允许将γ-玉米醇溶蛋白连接的结构摄取到植物细胞中。 或者，通过将γ-玉米醇溶蛋白肽与感兴趣的基因相互作用以形成γ-玉米醇溶蛋白连接的基因结构，可以在具有完整细胞壁的植物细胞中表达感兴趣的基因，从而允许γ-玉米醇溶蛋白连接的基因结构 进入植物细胞，并在植物细胞及其后代中表达感兴趣的基因。
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公开(公告)号：US08283522B2
公开(公告)日：2012-10-09
申请号：US12091896
申请日：2006-10-27
Applicant: Terry R. Wright , Justin M. Lira , Terence Anthony Walsh , Donald J. Merlo , Jayakumar P. Samuel , Gaofeng Lin
Inventor： Terry R. Wright , Justin M. Lira , Terence Anthony Walsh , Donald J. Merlo , Jayakumar P. Samuel , Gaofeng Lin
IPC: C12N15/82
CPC classification number: C12N15/8274 , C12N9/0071 , C12N15/8275
Abstract: The subject invention provides novel plants that are not only resistant to 2,4-D, but also to pyridyloxyacetate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention “stacked” together with one or more other herbicide resistance genes. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the development of, and controlling, strains of weeds that are resistant to one or more herbicides such as glyphosate. The preferred enzyme and gene for use according to the subject invention are referred to herein as AAD-12 (AryloxyAlkanoate Dioxygenase). This highly novel discovery is the basis of significant herbicide tolerant crop trait and selectable marker opportunities.
Abstract translation: 本发明提供了不仅对2,4-D有抗性的新型植物，而且还提供了对吡啶氧基乙酸酯除草剂的抗性。 迄今为止，没有任何期望或建议，通过引入单一基因可以产生具有这两种有利性质的植物。 本发明还包括产生本发明一种或多种与一种或多种除草剂抗性基因堆叠在一起的酶的植物。 本发明能够以新的方式使用除草剂的新型组合。 此外，本发明提供了防止对一种或多种除草剂如草甘膦具有抗性的杂草的发育和控制菌株的新方法。 根据本发明使用的优选酶和基因在本文中称为AAD-12（丙烯酰氧基烷酸酯加氧酶）。 这种高度新颖的发现是显着的除草剂耐受性作物性状和选择性标记机会的基础。
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