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    Human respiratory virus preparahons and processess
    2.
    发明授权
    Human respiratory virus preparahons and processess 失效
    人类呼吸道病毒预处理和过程

    公开(公告)号：US5716823A

    公开(公告)日：1998-02-10

    申请号：US854783

    申请日：1997-05-12

    申请人： Gail W. Wertz ,  Peter L. Collins

    发明人： Gail W. Wertz ,  Peter L. Collins

    IPC分类号： A61K38/00 ,  A61K39/00 ,  A61K39/155 ,  C07K14/135 ,  C12N15/00 ,  C12N1/12 ,  C12N1/20 ,  C12N7/00

    CPC分类号： C07K14/005 ,  A61K39/12 ,  A61K39/155 ,  A61K38/00 ,  A61K39/00 ,  C12N2760/18522 ,  C12N2760/18534

    摘要： This invention discloses compositions of DNA and proteins that are useful for preparing vaccines against human respiratory syncytial virus �HRSV!. The DNA compositions include structural genes coding for native structural viral proteins and immunogenic fragments of these proteins. Host cells transformed with the above DNA compositions are also disclosed. Vaccines made from the native structural viral proteins or immunogenic fragments are also disclosed as well as methods for protecting humans by inoculation with these vaccines.

    摘要翻译： 本发明公开了可用于制备针对人呼吸道合胞病毒（HRSV）的疫苗的DNA和蛋白质组合物。 DNA组合物包括编码天然结构病毒蛋白的结构基因和这些蛋白质的免疫原性片段。 还公开了用上述DNA组合物转化的宿主细胞。 还公开了由天然结构病毒蛋白质或免疫原性片段制备的疫苗以及通过接种这些疫苗来保护人的方法。

    Vaccines for human respiratory virus
    3.
    发明授权
    Vaccines for human respiratory virus 失效
    人类呼吸道病毒疫苗

    公开(公告)号：US5149650A

    公开(公告)日：1992-09-22

    申请号：US218737

    申请日：1988-07-13

    申请人： Gail W. Wertz ,  Peter L. Collins

    发明人： Gail W. Wertz ,  Peter L. Collins

    IPC分类号： A61K38/00 ,  A61K39/00 ,  A61K39/155 ,  C07K14/135

    CPC分类号： C07K14/005 ,  A61K39/12 ,  A61K39/155 ,  A61K38/00 ,  A61K39/00 ,  C12N2760/18522 ,  C12N2760/18534

    摘要： This invention discloses compositions of DNA and proteins that are useful for preparing vaccines against human respiratory syncytial virus [HRSV]. The DNA compositions include structural genes coding for native structural viral proteins and immunogenic fragments of these proteins. Host cells transformed with the above DNA compositions are also disclosed. Vaccines made from the native structural viral proteins or immunogenic fragments are also disclosed as well as methods for protecting humans by inoculation with these vaccines.

    摘要翻译： 本发明公开了可用于制备针对人呼吸道合胞病毒（HRSV）的疫苗的DNA和蛋白质组合物。 DNA组合物包括编码天然结构病毒蛋白的结构基因和这些蛋白质的免疫原性片段。 还公开了用上述DNA组合物转化的宿主细胞。 还公开了由天然结构病毒蛋白质或免疫原性片段制备的疫苗以及通过接种这些疫苗来保护人的方法。

    Prevention and treatment of respiratory tract disease
    4.
    发明授权
    Prevention and treatment of respiratory tract disease 失效
    预防和治疗呼吸道疾病

    公开(公告)号：US5716821A

    公开(公告)日：1998-02-10

    申请号：US316438

    申请日：1994-09-30

    申请人： Gail W. Wertz ,  Qingzhong Yu

    发明人： Gail W. Wertz ,  Qingzhong Yu

    IPC分类号： A61K39/00 ,  A61K48/00 ,  C07K14/08 ,  C12N15/86 ,  C12N7/00

    CPC分类号： C07K14/005 ,  C12N15/86 ,  A61K39/00 ,  A61K48/00 ,  C12N2760/18522 ,  C12N2760/18543

    摘要： Recombinant methods for recovering wildtype or engineered negative stranded, non-segmented RNA virus genomes containing non-coding 3' and 5' regions (e.g. leader or trailer regions) surrounding one, several or all of the genes of the virus or one or more heterologous gene(s) in the form of ribonucleocapsids containing N, P and L proteins, which are capable of replicating and assembling with the remaining structural proteins to bud and form virions, or which are only capable of infecting one cell, or are defective interfering particles, are disclosed. Novel vaccines, gene therapy vectors and antiviral compounds are also disclosed.

    摘要翻译： 用于恢复包含病毒的一个，几个或所有基因的一个或多个异源的包含非编码3'和5'区域（例如前导序列或尾部区域）的野生型或工程化的负链，非分段RNA病毒基因组的重组方法 含有N，P和L蛋白的核糖壳形式的基因，其能够与剩余的结构蛋白质复制并组装成芽并形成病毒体，或仅能够感染一个细胞，或是有缺陷的干扰颗粒 ，被披露。 还公开了新型疫苗，基因治疗载体和抗病毒化合物。

    Recombinant viruses of the paramyxoviridae family with heterologous envelope glycoproteins
    6.
    发明授权
    Recombinant viruses of the paramyxoviridae family with heterologous envelope glycoproteins 失效
    具有异源包膜糖蛋白的副粘病毒科的重组病毒

    公开(公告)号：US07588770B2

    公开(公告)日：2009-09-15

    申请号：US10575279

    申请日：2004-12-10

    申请人： Antonius G. P. Oomens ,  Gail W. Wertz ,  Alexander George Megaw

    发明人： Antonius G. P. Oomens ,  Gail W. Wertz ,  Alexander George Megaw

    IPC分类号： A61K39/38 ,  A61K39/12 ,  A61K39/295 ,  A61K39/155

    CPC分类号： C12N15/86 ,  A61K39/12 ,  A61K39/155 ,  A61K2039/5254 ,  A61K2039/5256 ,  C07K14/005 ,  C07K2319/00 ,  C12N7/00 ,  C12N2760/18522 ,  C12N2760/18534 ,  C12N2760/18543 ,  C12N2760/18545 ,  C12N2760/18562 ,  C12N2810/60

    摘要： The invention provides recombinant viruses comprising heterologous envelope proteins capable of mediating entry of the recombinant viruses into host cells. In one embodiment, a recombinant virus of the invention is a member of the Paramyx-ovirzdae family (e.g., a respiratory syncytial virus), and the heterologous envelope protein includes the ectodomain of a baculovirus envelope protein (e.g., the GP64 protein). In some cases, the heterologous envelope protein is provided in addition to homologous envelope proteins) which may or may not be functional. The heterologous protein can provide the recombinant virus with enhanced stability (e.g., at 4° C., 22° C., or 37° C.), and allows production of high-titer virus stocks. The heterologous protein can also impart temperature sensitivity to the replication of the recombinant virus. In addition, the recombinant virus can be designed to be infectious but incapable of spreading between host cells by providing the heterologous protein by complementation in trans. These features attenuate the disease-causing potential of the recombinant virus, therefore increasing its safety of use as vaccines.

    摘要翻译： 本发明提供了包含能够介导重组病毒进入宿主细胞的异源包膜蛋白的重组病毒。 在一个实施方案中，本发明的重组病毒是副粘病毒家族（例如呼吸道合胞病毒）的成员，异源包膜蛋白包括杆状病毒包膜蛋白（例如GP64蛋白）的胞外域。 在一些情况下，异源包膜蛋白除了同源包膜蛋白外还提供），其可以是或不是功能性的。 异源蛋白质可以提供具有增强的稳定性（例如在4℃，22℃或37℃）的重组病毒，并且允许产生高滴度病毒种群。 异源蛋白质还可以赋予重组病毒复制的温度敏感性。 此外，重组病毒可被设计为感染性的，但不能通过在反式中互补提供异源蛋白质而在宿主细胞之间扩散。 这些特征减轻重组病毒的致病潜力，从而增加其作为疫苗使用的安全性。

    Attenuation of negative stranded RNA viruses by rearrangement of genes and uses thereof
    8.
    发明授权
    Attenuation of negative stranded RNA viruses by rearrangement of genes and uses thereof 失效
    通过基因重排减少负链RNA病毒及其用途

    公开(公告)号：US6136585A

    公开(公告)日：2000-10-24

    申请号：US71606

    申请日：1998-05-01

    申请人： L. Andrew Ball ,  Gail W. Wertz

    发明人： L. Andrew Ball ,  Gail W. Wertz

    IPC分类号： C12N15/09 ,  A61K39/00 ,  A61K39/12 ,  A61K39/155 ,  A61K39/205 ,  C12N7/04 ,  C12N15/86

    CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K39/205 ,  C12N15/86 ,  A61K2039/5254 ,  A61K2039/543 ,  C12N2760/20234 ,  C12N2760/20243 ,  C12N2760/20261

    摘要： Provided is a method of attenuating a virus of the order Mononegavirales, comprising the step of: rearranging said virus' gene order by moving a gene essential for RNA replication away from its wild-type 3' promoter proximal position site, wherein said gene is an essential limiting factor for genome replication and is placed in the next to last position in the gene order. Also provided is a method of making an attenuated virus useful for a vaccine, comprising the steps of: rearranging said virus' gene order by moving a gene essential for RNA replication away from its wild-type 3' promoter proximal position site, wherein a gene which is a n essential limiting factor for genome replication is placed in the next to last position in the gene order; and placing a gene coding for an immune response inducing antigen in the position closest to the 3' end of the gene order. Also provided is a method of attenuating a virus of the order Mononegavirales, comprising the step of: rearranging said virus' gene order.

    摘要翻译： 本发明提供一种减毒单宁病毒病毒的方法，包括以下步骤：通过将RNA复制所必需的基因从其野生型3'启动子近端位点移动而重新排列所述病毒基因顺序，其中所述基因是 基因组复制的基本限制因素，并被置于下一个基因顺序的最后位置。 还提供了制备可用于疫苗的减毒病毒的方法，包括以下步骤：通过将RNA复制必需的基因移动离开其野生型3'启动子近端位点来重排所述病毒的基因顺序，其中基因 这是基因组复制的基本限制因素放在基因顺序的最后一个位置; 并将编码免疫应答诱导抗原的基因置于最靠近基因顺序3'末端的位置。 还提供了减毒单宁病毒的病毒的方法，其包括以下步骤：重新排列所述病毒的基因顺序。

    Nucleotide sequences encoding bovine respiratory syncytial virus immunogenic proteins
    9.
    发明授权
    Nucleotide sequences encoding bovine respiratory syncytial virus immunogenic proteins 失效
    编码牛呼吸道合胞病毒免疫原性蛋白的核苷酸序列

    公开(公告)号：US6060280A

    公开(公告)日：2000-05-09

    申请号：US118148

    申请日：1993-09-08

    申请人： Gail W. Wertz ,  Robert Lerch

    发明人： Gail W. Wertz ,  Robert Lerch

    IPC分类号： A61K39/155 ,  A61K38/00 ,  A61K39/00 ,  A61K39/12 ,  A61K39/395 ,  A61P11/00 ,  A61P31/12 ,  A61P37/04 ,  C07K14/00 ,  C07K14/01 ,  C07K14/115 ,  C07K14/135 ,  C07K16/00 ,  C07K16/08 ,  C07K16/10 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N7/00 ,  C12N7/04 ,  C12N15/09 ,  C12P21/02 ,  C12P21/08 ,  C12Q1/68 ,  C12Q1/70 ,  C12R1/01 ,  C12R1/645 ,  C12R1/91 ,  C12R1/93 ,  G01N33/15 ,  G01N33/50 ,  G01N33/53 ,  G01N33/566 ,  G01N33/569 ,  G01N33/577 ,  C12P21/06 ,  C07H21/02 ,  C12N5/00 ,  C12N15/00

    CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  C12N2760/18522

    摘要： The present invention relates to recombinant DNA molecules which encode bovine respiratory syncytial (BRS) virus proteins, to BRS virus proteins, and peptides and to recombinant BRS virus vaccines produced therefrom. It is based, in part, on the cloning of substantially full length cDNAs which encode the entire BRS virus C, F, and N proteins. According to particular embodiments of the invention, DNA encoding a BRS virus protein or peptide may be used to diagnose BRS virus infection, or, alternatively, may be inserted into an expression vector, including, but not limited to, vaccinia virus as well as bacterial, yeast, insect, or other vertebrate vectors. These expression vectors may be utilized to produce the BRS virus protein or peptide in quantity; the resulting substantially pure viral peptide or protein may be incorporated into subunit vaccine formulations or may be used to generate monoclonal or polyclonal antibodies which may be utilized in diagnosis of BRS virus infection or passive immunization. In additional embodiments, BRS virus protein sequence provided by the invention may be used to produce synthetic peptides or proteins which may be utilized in subunit vaccines,or polyclonal or monoclonal antibody production. Alternatively, a nonpathogenic expression vector containing the genes, parts of the genes, any combination of the genes, or parts thereof may itself be utilized as a recombinant virus vaccine.

    摘要翻译： 本发明涉及编码牛呼吸道合胞体（BRS）病毒蛋白质，BRS病毒蛋白质的重组DNA分子，以及由其产生的重组BRS病毒疫苗。 其部分基于克隆编码整个BRS病毒C，F和N蛋白的基本上全长的cDNA。 根据本发明的具体实施方案，编码BRS病毒蛋白或肽的DNA可用于诊断BRS病毒感染，或者可以将其插入到表达载体中，包括但不限于痘苗病毒以及细菌 ，酵母，昆虫或其他脊椎动物载体。 这些表达载体可以用来量产BRS病毒蛋白或肽; 所得到的基本上纯的病毒肽或蛋白质可以并入亚单位疫苗制剂中，或可用于产生可用于诊断BRS病毒感染或被动免疫的单克隆或多克隆抗体。 在另外的实施方案中，本发明提供的BRS病毒蛋白质序列可用于产生可用于亚单位疫苗或多克隆或单克隆抗体产生的合成肽或蛋白质。 或者，含有基因，部分基因，基因的任何组合或其部分的非致病性表达载体本身可以用作重组病毒疫苗。

    Nucleotide sequences encoding bovine respiratory syncytial virus immunogenic proteins
    10.
    发明授权
    Nucleotide sequences encoding bovine respiratory syncytial virus immunogenic proteins 失效
    编码牛呼吸道合胞病毒免疫原性蛋白的核苷酸序列

    公开(公告)号：US06730305B1

    公开(公告)日：2004-05-04

    申请号：US09567458

    申请日：2000-05-08

    申请人： Gail W. Wertz ,  Robert Lerch

    发明人： Gail W. Wertz ,  Robert Lerch

    IPC分类号： A61K39155

    CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  A61K39/12 ,  A61K39/155 ,  A61K2039/5256 ,  C12N2710/24143 ,  C12N2760/18522 ,  C12N2760/18534

    摘要： The present invention relates to recombinant DNA molecules which encode bovine respiratory syncytial (BRS) virus proteins, to BRS virus proteins, and peptides and to recombinant BRS virus vaccines produced therefrom. It is based, in part, on the cloning of substantially full length cDNAs which encode the entire BRS virus G, F, and N proteins. According to particular embodiments of the invention, DNA encoding a BRS virus protein or peptide may be used to diagnose BRS virus infection, or, alternatively, may be inserted into an expression vector, including, but not limited to, vaccinia virus as well as bacterial, yeast, insect, or other vertebrate vectors. These expression vectors may be utilized to produce the BRS virus protein or peptide in quantity; the resulting substantially pure viral peptide or protein may be incorporated into subunit vaccine formulations or may be used to generate monoclonal or polyclonal antibodies which may be utilized in diagnosis of BRS virus infection or passive immunization. In additional embodiments, BRS virus protein sequence provided by the invention may be used to produce synthetic peptides or proteins which may be utilized in subunit vaccines, or polyclonal or monoclonal antibody production. Alternatively, a nonpathogenic expression vector containing the genes, parts of the genes, any combination of the genes, or parts thereof may itself be utilized as a recombinant virus vaccine.

    摘要翻译： 本发明涉及编码牛呼吸道合胞体（BRS）病毒蛋白质，BRS病毒蛋白质的重组DNA分子，以及由其产生的重组BRS病毒疫苗。 其部分基于克隆编码整个BRS病毒G，F和N蛋白的基本上全长的cDNA。 根据本发明的具体实施方案，编码BRS病毒蛋白或肽的DNA可用于诊断BRS病毒感染，或者可以将其插入到表达载体中，包括但不限于痘苗病毒以及细菌 ，酵母，昆虫或其他脊椎动物载体。 这些表达载体可以用来量产BRS病毒蛋白或肽; 所得到的基本上纯的病毒肽或蛋白质可以并入亚单位疫苗制剂中，或可用于产生可用于诊断BRS病毒感染或被动免疫的单克隆或多克隆抗体。 在另外的实施方案中，本发明提供的BRS病毒蛋白质序列可用于产生可用于亚单位疫苗或多克隆或单克隆抗体产生的合成肽或蛋白质。 或者，含有基因，部分基因，基因的任何组合或其部分的非致病性表达载体本身可以用作重组病毒疫苗。