公开(公告)号：US07041489B2

公开(公告)日：2006-05-09

申请号：US10262238

申请日：2002-10-01

申请人： Gail W. Wertz ,  Alexander George Megaw ,  A. Tom Oomens

发明人： Gail W. Wertz ,  Alexander George Megaw ,  A. Tom Oomens

IPC分类号： C12N7/00

CPC分类号： C07K14/005 ,  A61K2039/5254 ,  C07K2319/00 ,  C07K2319/01 ,  C07K2319/60 ,  C12N15/86 ,  C12N2760/18522 ,  C12N2760/18543 ,  C12N2760/18545 ,  C12N2760/20222 ,  C12N2810/60 ,  C12N2810/6081

摘要： The present invention provides recombinant respiratory syncytial viruses (RSV) in which all of the surface glycoprotein genes encoding the attachment protein G, the fusion protein F, and the Small Hydrophobic protein SH are deleted. The genes are replaced by a chimeric gene encoding a heterologous entry protein derived from the Vesicular Stomatitis Virus G protein or GP64 of baculovirus. Alternatively, the replacement proteins are provided in trans. Marker genes such as those encoding β-glucuronidase (GUS) and green fluorescent protein (EGFP) are also added to the upstream and downstream side of the hybrid gene for easy detection. These infectious recombinant respiratory syncytial viruses offer alternatives and improvements as vaccine candidates.