公开(公告)号：US20220033889A1

公开(公告)日：2022-02-03

申请号：US17282630

申请日：2019-10-04

申请人： First Light Diagnostics, Inc.

发明人： Don Straus

IPC分类号： C12Q1/6841 ,  C12Q1/689 ,  C12Q1/20 ,  G01N33/543

摘要： The invention provides a version of fluorescent in situ hybridization (FISH) in which all the steps are performed at physiological temperatures, i.e., body temperature, to detect and identify pathogenic bacteria in clinical samples. Methods of the invention use species-specific fluorescent probes to label clinically important infectious bacteria. A sample such as a urine sample is loaded into a cartridge, fluorescently labeled, and imaged with a microscope. Labelled bacteria are pulled down onto an imaging surface and a dye cushion is used to keep unbound probes off of the imaging surface. A microscopic image of the surface shows whether and in what quantities the infectious bacteria are present in the clinical sample.

公开(公告)号：US20230241603A1

公开(公告)日：2023-08-03

申请号：US17282623

申请日：2019-10-04

申请人： First Light Diagnostics, Inc.

发明人： Don Straus

IPC分类号： B01L3/00

CPC分类号： B01L3/502715 ,  B01L3/502761 ,  B01L3/502738 ,  B01L2200/0647 ,  B01L2300/18 ,  B01L2200/021 ,  B01L2300/0864 ,  B01L2400/043 ,  B01L2400/065 ,  B01L2200/16

摘要： The disclosure provides cartridges that are pre-loaded with reagents for performing antimicrobial susceptibility testing (AST) and FISH testing. Cartridges of the disclosure include various incubation wells loaded with different antimicrobial agents for differential growth analysis. Imaging wells with species-specific microbial probes, fluorescent tags, magnetic particles and dye-cushion layers allow for tagging and imaging of target microbes for differential growth analysis.

公开(公告)号：US20230235411A1

公开(公告)日：2023-07-27

申请号：US17282633

申请日：2019-10-04

申请人： First Light Diagnostics, Inc.

发明人： Don Straus

IPC分类号： C12Q1/689 ,  C12Q1/6841 ,  C12Q1/20

CPC分类号： C12Q1/689 ,  C12Q1/6841 ,  C12Q1/20

摘要： The invention provides systems and methods for rapid automated identification of microbes and antimicrobial susceptibility testing (AST) directly from a patient specimen, without specimen preparation. Specimens are loaded into an analytical cartridge for processing. Analytical cartridges are preloaded with species-specific labels that are used to identify and enumerate microbes in the specimen. Instruments, such as analyzers can be used to interact with analytical cartridges to carry out methods of the invention all within the cartridge.

公开(公告)号：US20230120417A1

公开(公告)日：2023-04-20

申请号：US18082992

申请日：2022-12-16

申请人： First Light Diagnostics, Inc.

发明人： Greg Yantz ,  Don Straus ,  Gordon Siek ,  Damon Dehart

IPC分类号： B01L3/00 ,  A61B5/157 ,  G01N21/03 ,  G01N21/25 ,  G01N21/64 ,  G01N33/543 ,  A61B5/15 ,  A61B5/151

摘要： The invention provides devices that improve tests for detecting specific cellular, viral, and molecular targets in clinical, industrial, or environmental samples. The invention permits efficient detection of individual microscopic targets at low magnification for highly sensitive testing. The invention does not require washing steps and thus allows sensitive and specific detection while simplifying manual operation and lowering costs and complexity in automated operation. In short, the invention provides devices that can deliver rapid, accurate, and quantitative, easy-to-use, and cost-effective tests.

公开(公告)号：US11583853B2

公开(公告)日：2023-02-21

申请号：US16543028

申请日：2019-08-16

申请人： First Light Diagnostics, Inc.

发明人： Greg Yantz ,  Don Straus ,  Gordon Siek ,  Damon Dehart

IPC分类号： B01L3/00 ,  A61B5/157 ,  G01N21/03 ,  G01N21/25 ,  G01N21/64 ,  G01N33/543 ,  A61B5/15 ,  A61B5/151 ,  B01L7/00 ,  B01L9/00 ,  A61B5/117

摘要： The invention provides devices that improve tests for detecting specific cellular, viral, and molecular targets in clinical, industrial, or environmental samples. The invention permits efficient detection of individual microscopic targets at low magnification for highly sensitive testing. The invention does not require washing steps and thus allows sensitive and specific detection while simplifying manual operation and lowering costs and complexity in automated operation. In short, the invention provides devices that can deliver rapid, accurate, and quantitative, easy-to-use, and cost-effective tests.

公开(公告)号：US20210349082A1

公开(公告)日：2021-11-11

申请号：US17282627

申请日：2019-10-04

申请人： First Light Diagnostics, Inc.

发明人： Don Straus

IPC分类号： G01N33/543 ,  G01N35/02 ,  C12Q1/18 ,  C12Q1/689 ,  G01N21/64 ,  B01L3/00

摘要： The invention provides multi-function instruments for automatically and simultaneously carrying out a variety of tests including identification of targets in specimens and antimicrobial susceptibility testing thereof. Application-specific cartridges are pre-loaded with all required reagents and allow for tests to be performed in a single testing device with no specimen preparation to, for example, rapidly detect infections, identify infectious pathogens, and analyze their susceptibility to various antimicrobial agents. Instruments include various stations for carrying out test steps that can be randomly accessed in any order dictated by the computer-controlled instrument. A carousel stores and incubates cartridges and, with a mechanical conveyor arm, transfers the cartridges between the required stations to carry out the tests. A plurality of different tests may be performed on a plurality of targets within an instrument, and the plurality of targets may be disposed in a single cartridge.