摘要:
A method for the recombinant production of a polypeptide in a eukaryotic host cell modified in the citrate cycle is described, wherein the method comprises cultivating the eukaryotic host cell in a suitable medium under conditions which allow the expression of the polypeptide, wherein the content of dissolved CO2 (pCO2) in the medium is maintained at a constant value in the range of 10% to 20%.
摘要:
An agitator for use on an agitator shaft having an axis of rotation. The agitator has a blade mount configured and dimensioned to receive the agitator shaft and being further configured and dimensioned so as to hold a plurality of agitator blades radially with respect to the axis of rotation. Each agitator blade has a plurality of segments, including an upper segment relative to the axis of rotation and a lower segment relative to the axis of rotation. The segments are inclined at an acute angle relative to the axis of rotation in the direction of rotation, wherein the acute angle changes continuously or stepwise from about 25° at the upper segment to about 65° at the lower segment.
摘要:
A method for the recombinant production of a polypeptide in a eukaryotic host cell modified in the citrate cycle is described, wherein the method comprises cultivating the eukaryotic host cell in a suitable medium under conditions which allow the expression of the polypeptide, wherein the content of dissolved CO2 (pCO2) in the medium is maintained at a constant value in the range of 10% to 20%.
摘要:
An agitator for use on an agitator shaft having an axis of rotation. The agitator has a blade mount configured and dimensioned to receive the agitator shaft and being further configured and dimensioned so as to hold a plurality of agitator blades radially with respect to the axis of rotation. Each agitator blade has a plurality of segments, including an upper segment relative to the axis of rotation and a lower segment relative to the axis of rotation. The segments are inclined at an acute angle relative to the axis of rotation in the direction of rotation, wherein the acute angle changes continuously or stepwise from about 25° at the upper segment to about 65° at the lower segment.
摘要:
The present invention is a process for converting trimethylhydroquinone diacetate (TMHQ-DA) into trimethylhydroquinone-1-monoacetate (TMHQ-1-MA) by contacting TMHQ-DA with a lipase to effect an enzymatic monosaponification of the TMHQ-DA. Also provided are methods of making (all-rac)-&agr;-tocopherol and (all-rac)-&agr;-tocopherol acetate.