公开(公告)号：US20060019352A1

公开(公告)日：2006-01-26

申请号：US10724598

申请日：2003-12-01

Applicant: Francis Blanche ,  Beatrice Cameron ,  Joel Crouzet ,  Laurent Debussche ,  Sophie Levy Schil ,  Denis Thibaut

Inventor： Francis Blanche ,  Beatrice Cameron ,  Joel Crouzet ,  Laurent Debussche ,  Sophie Levy Schil ,  Denis Thibaut

IPC: C12P19/42 ,  C12N1/20

CPC classification number: C12N9/00 ,  C12N15/52 ,  C12P19/42

Abstract: Novel polypeptides involved in the biosynthesis of cobalamines and/or cobamides, in particular coenzyme B12, genetic material responsible for expressing these polypeptides, and a method for preparing them, are described. A method for amplifying the production of cobalamines, and particularly coenzyme B12, using recombinant DNA techniques, are also described.

Abstract translation: 描述了参与钴胺和/或鸟苷酰胺（特别是辅酶B 12）的生物合成的新型多肽，负责表达这些多肽的遗传物质及其制备方法。 还描述了使用重组DNA技术扩增钴胺，特别是辅酶B 12的生产的方法。

公开(公告)号：US06833382B2

公开(公告)日：2004-12-21

申请号：US09987614

申请日：2001-11-15

Applicant: Véronique Blanc ,  Denis Thibaut ,  Nathalie Bamas-Jacques ,  Francis Blanche ,  Joël Crouzet ,  Jean-Claude Barriere ,  Laurent Debussche ,  Alain Famechon ,  Jean-Marc Paris ,  Gilles Dutruc-Rosset

Inventor： Véronique Blanc ,  Denis Thibaut ,  Nathalie Bamas-Jacques ,  Francis Blanche ,  Joël Crouzet ,  Jean-Claude Barriere ,  Laurent Debussche ,  Alain Famechon ,  Jean-Marc Paris ,  Gilles Dutruc-Rosset

IPC: A61K3140

CPC classification number: C07D295/155 ,  A61K38/00 ,  C07C229/36 ,  C07C229/42 ,  C07C233/15 ,  C07C233/25 ,  C07C323/63 ,  C07K7/06 ,  C12N15/52 ,  C12N15/76

Abstract: Novel group B streptogramin-like compounds of general formula (I), and a method for preparing streptogramines by mutasynthesis using a mutated micro-organism to influence the biosynthesis of at least one of the precursors of group B streptogramins are disclosed. Novel nucleotide sequences involved in the biosynthesis of said precursors, and their uses, are also disclosed.

Abstract translation: 公开了通式（I）的新型B族链霉素样化合物，以及通过使用突变微生物进行突变的制备链霉菌素以影响至少一种B族链霉素前体的生物合成的方法。 还公开了涉及所述前体的生物合成的新型核苷酸序列及其用途。

公开(公告)号：US06670157B1

公开(公告)日：2003-12-30

申请号：US09635359

申请日：2000-08-09

Applicant: Veronique Blanc ,  Francis Blanche ,  Joel Crouzet ,  Nathalie Jacques ,  Patricia Lacroix ,  Denis Thibaut ,  Monique Zagorec ,  Laurent Debussche ,  Valerie De Crecy-Lagard

Inventor： Veronique Blanc ,  Francis Blanche ,  Joel Crouzet ,  Nathalie Jacques ,  Patricia Lacroix ,  Denis Thibaut ,  Monique Zagorec ,  Laurent Debussche ,  Valerie De Crecy-Lagard

IPC: C12P1714

CPC classification number: C12R1/465 ,  C07K5/06182 ,  C07K7/06 ,  C12N9/0012 ,  C12N9/1085 ,  C12N9/93 ,  C12N15/52 ,  C12P17/14

Abstract: The invention concerns methods for preparing pristinamycin IIB and methods for preparing modified pristinamycin IIB.

Abstract translation: 本发明涉及制备普瑞霉素IIB的方法和制备改性的原始霉素霉素IIB的方法。

公开(公告)号：US06656709B1

公开(公告)日：2003-12-02

申请号：US08426630

申请日：1995-04-21

Applicant: Francis Blanche ,  Beatrice Cameron ,  Joel Crouzet ,  Laurent Debussche ,  Sophie Levy Schil ,  Denis Thibaut

Inventor： Francis Blanche ,  Beatrice Cameron ,  Joel Crouzet ,  Laurent Debussche ,  Sophie Levy Schil ,  Denis Thibaut

IPC: C12P1942

CPC classification number: C12N9/00 ,  C12N15/52 ,  C12P19/42

Abstract: Novel polypeptides involved in the biosynthesis of cobalamines and/or cobamides, in particular coenzyme B12, genetic material responsible for expressing these polypeptides, and a method for preparing them, are described. A method for amplifying the production of cobalamines, and particularly coenzyme B12, using recombinant DNA techniques, are also described.

Abstract translation: 描述了涉及钴胺和/或鸟苷酰胺的生物合成的新型多肽，特别是辅酶B12，负责表达这些多肽的遗传物质及其制备方法。 还描述了使用重组DNA技术扩增钴胺，特别是辅酶B12的生产的方法。

公开(公告)号：US06352839B1

公开(公告)日：2002-03-05

申请号：US08765907

申请日：1997-03-20

Applicant: Véronique Blanc ,  Denis Thibaut ,  Nathalie Bamas-Jacques ,  Francis Blanche ,  Joël Crouzet ,  Jean-Claude Barriere ,  Laurent Debussche ,  Alain Famechon ,  Jean-Marc Paris ,  Gilles Dutruc-Rosset

Inventor： Véronique Blanc ,  Denis Thibaut ,  Nathalie Bamas-Jacques ,  Francis Blanche ,  Joël Crouzet ,  Jean-Claude Barriere ,  Laurent Debussche ,  Alain Famechon ,  Jean-Marc Paris ,  Gilles Dutruc-Rosset

IPC: C12P100

CPC classification number: C07D295/155 ,  A61K38/00 ,  C07C229/36 ,  C07C229/42 ,  C07C233/15 ,  C07C233/25 ,  C07C323/63 ,  C07K7/06 ,  C12N15/52 ,  C12N15/76

Abstract: The invention provides a method for preparing streptogramins using genetically-modified microorganisms to influence the biosynthesis of at least one of the precursors of the group B streptogramins. Cultures of the genetically-modified microorganisms are supplemented with a least one precursor that is different from the streptogramin precursor whose biosynthesis is altered and the streptogramins produced are recovered.

Abstract translation: 本发明提供了使用遗传修饰的微生物来制备链霉素的方法，所述基因修饰的微生物影响B群链霉素的至少一种前体的生物合成。 对经遗传修饰的微生物的培养物补充有至少一种不同于生物合成改变的链霉素前体的前体，并且生成的链霉蛋白酶被回收。

公开(公告)号：US6089249A

公开(公告)日：2000-07-18

申请号：US271863

申请日：1999-03-18

Applicant: Denis Thibaut ,  Jules-Joseph Van Schaftingen ,  Paul Wouters

Inventor： Denis Thibaut ,  Jules-Joseph Van Schaftingen ,  Paul Wouters

IPC: B60K15/03 ,  B60K15/035 ,  B60K15/077 ,  F02M37/00 ,  F16R24/04

CPC classification number: B60K15/035 ,  Y10T137/3099 ,  Y10T137/86212 ,  Y10T137/86324

Abstract: Venting circuit for a liquid tank, the upper wall of which comprises at least two regions liable to contain pockets of gas, the said circuit consisting of at least one ventilation tubing connecting ventilation points located in the upper part of the regions and a vent, and having at least one siphon-shaped lower part connected via a pipe to a point under vacuum inside the tank so as to allow the liquid present in the venting circuit to be removed, the vacuum being created by the pump for drawing off the liquid.

Abstract translation: 用于液体罐的通风回路包括至少两个易于容纳气体袋的区域，所述电路由至少一个通气管道组成，所述至少一个通风管道连接位于该区域上部的通风点和通风口，以及 具有至少一个虹吸下部通过管道连接到罐内的真空点，以便允许排出回路中存在的液体被除去，由泵产生真空以抽出液体。

公开(公告)号：US07090999B2

公开(公告)日：2006-08-15

申请号：US10716803

申请日：2003-11-20

Applicant: Veronique Blanc ,  Francis Blanche ,  Joel Crouzet ,  Nathalie Jacques ,  Patricia Lacroix ,  Denis Thibaut ,  Monique Zagorec ,  Laurent Debussche ,  Valerie De Crecy-Lagard

Inventor： Veronique Blanc ,  Francis Blanche ,  Joel Crouzet ,  Nathalie Jacques ,  Patricia Lacroix ,  Denis Thibaut ,  Monique Zagorec ,  Laurent Debussche ,  Valerie De Crecy-Lagard

IPC: C12P17/00

CPC classification number: C12R1/465 ,  C07K5/06182 ,  C07K7/06 ,  C12N9/0012 ,  C12N9/1085 ,  C12N9/93 ,  C12N15/52 ,  C12P17/14

Abstract: The invention concerns methods for preparing pristinamycin IIA or IIB.

Abstract translation: 本发明涉及制备嘌呤霉素IIA或IIB的方法。

公开(公告)号：US20050038255A1

公开(公告)日：2005-02-17

申请号：US10479719

申请日：2002-06-10

Applicant: Denis Thibaut ,  Volker Doring ,  Philippe Marliere

Inventor： Denis Thibaut ,  Volker Doring ,  Philippe Marliere

IPC: C12N15/09 ,  C07B53/00 ,  C07D211/60 ,  C07D241/04 ,  C07D279/12 ,  C12N1/21 ,  C12N9/88 ,  C12N15/60 ,  C12P13/04 ,  C12P17/10 ,  C12P17/12 ,  C12P41/00 ,  C07D211/30

CPC classification number: C12N9/88 ,  C12P13/04 ,  C12P17/10 ,  C12P17/12 ,  C12P41/006

Abstract: The invention concerns a method for producing a cyclic L-amino acid of formula (I), characterised in that it consists in reacting a L-diamino acid of formula (II) or an enantiomeric mixture comprising such a L-diamino acid and a corresponding D-diamino acid in variable proportions, in the presence of an ornithine cyclodeaminase or a polypeptide homologous to the ornithine cyclodeaminase.

Abstract translation: 本发明涉及一种制备式（I）的环状L-氨基酸的方法，其特征在于其包括使式（II）的L-二氨基酸或包含这样的L-二氨基酸的对映异构体混合物与相应的 在鸟氨酸环脱氨酶或与鸟氨酸环脱氨酶同源的多肽的存在下，可变比例的D-二氨基酸。

公开(公告)号：US07604970B2

公开(公告)日：2009-10-20

申请号：US10603282

申请日：2003-06-25

Applicant: Nathalie Bamas-Jacques ,  Denis Thibaut ,  Alain Famechon

Inventor： Nathalie Bamas-Jacques ,  Denis Thibaut ,  Alain Famechon

IPC: C12N9/10 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC classification number: C07K5/021 ,  A61K38/00 ,  C12N9/1007

Abstract: The present invention relates to novel variants of the PapM polypeptide of bacteria of the Streptomyces genus which possess a substrate selectivity and/or an improved effectiveness compared to the wild-type polypeptide. It also relates to the nucleic acids encoding these variants, to the microorganisms which incorporate these nucleic acids and to their use for producing B components of Streptogramins.

Abstract translation: 本发明涉及与野生型多肽相比具有底物选择性和/或改善效力的链霉菌属的细菌的PapM多肽的新变体。 它还涉及编码这些变体的核酸，结合到这些核酸的微生物以及它们用于产生Streptogramins的B组分的用途。

公开(公告)号：US6077699A

公开(公告)日：2000-06-20

申请号：US510646

申请日：1995-08-03

Applicant: Veronique Blanc ,  Francis Blanche ,  Joel Crouzet ,  Nathalie Jacques ,  Patricia Lacroix ,  Denis Thibaut ,  Monique Zagorec ,  Laurent Debussche ,  Valerie De Crecy-Lagard

Inventor： Veronique Blanc ,  Francis Blanche ,  Joel Crouzet ,  Nathalie Jacques ,  Patricia Lacroix ,  Denis Thibaut ,  Monique Zagorec ,  Laurent Debussche ,  Valerie De Crecy-Lagard

IPC: C07K5/078 ,  C07K7/06 ,  C12N9/00 ,  C12N9/06 ,  C12N9/10 ,  C12N15/52 ,  C07H21/04 ,  C12N1/20

CPC classification number: C12N9/0012 ,  C07K5/06182 ,  C07K7/06 ,  C12N15/52 ,  C12N9/1085 ,  C12N9/93 ,  C12R1/465

Abstract: Disclosed is the nucleic acid sequences for polypeptides and enzymes involved in the biosynthesis of streptogramins from Streptomyces sp. and the corresponding amino acid sequences. Also disclosed are recombinant vectors and cells as well as methods of making the polypeptides, enzymes and streptogramins using the recombinant host cells.

Abstract translation: 公开了涉及从链霉菌属（Streptomyces sp。）生物合成链霉素的多肽和酶的核酸序列。 和相应的氨基酸序列。 还公开了重组载体和细胞以及使用重组宿主细胞制备多肽，酶和链霉素的方法。