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1.发明授权Peptides which elicit a high neutralizing antibody titer, cytotoxic T lymphocyte response and T helper cell response in a broad range of MHC type recipients 失效在广泛的MHC型受体中引发高中和抗体滴度，细胞毒性T淋巴细胞反应和T辅助细胞应答的肽公开(公告)号：US07094405B1
公开(公告)日：2006-08-22
申请号：US09455076
申请日：1999-12-06
IPC分类号： A61K39/21
CPC分类号： C07K14/005 , A61K38/03 , A61K38/55 , A61K38/556 , A61K39/00 , A61K39/12 , A61K39/21 , A61K2039/545 , A61K2039/55516 , A61K2039/55566 , A61K2039/55577 , A61K2039/57 , A61K2039/645 , C07K2319/00 , C12N2740/16122 , C12N2740/16134 , Y02A50/412 , A61K2300/00
摘要： Peptide constructs comprised of multideterminant T helper peptides from the envelope glycoprotein of HIV previously identified to induce proliferative responses in four different haplotypes of mice and IL-2 responses in 52-73% of HIV positive, flu positive patients (cluster peptides), were co-linearly synthesized with the peptide 18 of the V3 loop of HIV-1 gp 160, corresponding to the principal neutralizing determinant of HIV-IIIB and also shown to contain a dominant CTL epitope. Cognate help for peptide 18 antibody was elicited following a single immunization in all strains of mice which had previously responded to a T cell epitope encompassed by the peptides. In two strains of mice, the level of neutralizing antibody achieved was comparable to levels adequate for protection from homologous viral challenge in chimpanzees. After a single boost, much higher antibody titers for 90% neutralization in the range of 1:1000 to 1:16,000 were achieved. Spleen cells from mice of three distinct MHC haplotypes sharing the Dd class I MHC molecule but with different class II molecules, immunized with the compound peptides, exhibited enhanced gp160-specific CTL activity.
摘要翻译： 由先前鉴定为在四种不同单倍型小鼠中诱导增殖反应的HIV的多重决定子T辅助肽和52-73％的HIV阳性患者（簇肽）中的IL-2应答组成的多肽构建体由co 与HIV-1 gp 160的V3环的肽18线性合成，对应于HIV-IIIB的主要中和决定簇，并且还显示含有显性CTL表位。 在先前对肽包含的T细胞表位作出反应的所有小鼠菌株中进行单次免疫后，引发了针对肽18抗体的协同帮助。 在两种小鼠中，达到的中和抗体水平与足以保护黑猩猩同源病毒攻击的水平相当。 在单次加强后，实现了在1:1000至1：16,000范围内高达90％中和的高得多的抗体滴度。 来自共享第一类MHC分子但具有用化合物肽免疫的不同II类分子的三种不同MHC单元型的小鼠的脾细胞表现出增强的gp160特异性CTL活性。
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2.发明授权Multideterminant peptides that elicit helper T-lymphocyte cytotoxic T-lymphocyte and neutralizing antibody responses against HIV-1 失效引起辅助T淋巴细胞细胞毒性T淋巴细胞和中和抗HIV-1抗体的多决定肽公开(公告)号：US06294322B1
公开(公告)日：2001-09-25
申请号：US08060988
申请日：1993-05-14
IPC分类号： C12Q170
CPC分类号： C07K14/005 , A61K38/03 , A61K38/55 , A61K38/556 , A61K39/00 , A61K39/12 , A61K39/21 , A61K2039/545 , A61K2039/55516 , A61K2039/55566 , A61K2039/55577 , A61K2039/57 , A61K2039/645 , C07K2319/00 , C12N2740/16122 , C12N2740/16134 , Y02A50/412 , A61K2300/00
摘要： Peptide constructs comprised of multideterminant T helper peptides from the envelope glycoprotein of HIV previously identified to induce proliferative responses in four different haplotypes of mice and IL-2 responses in 52-73% of HIV positive, flu positive patients (cluster peptides), were co-linearly synthesized with the peptide 18 of the V3 loop of HIV-1 gp 160, corresponding to the principal neutralizing determinant of HIV-IIIB and also shown to contain a dominant CTL epitope. Cognate help for peptide 18 antibody was elicited following a single immunization in all strains of mice which had previously responded to a T cell epitope encompassed by the peptides. In two strains of mice, the level of neutralizing antibody achieved was comparable to levels adequate for protection from homologous viral challenge in chimpanzees. After a single boost, much higher antibody titers for 90% neutralization in the range of 1:1000 to 1:16,000 were achieved. Spleen cells from mice of three distinct MHC haplotypes sharing the Dd class I MHC molecule but with different class II molecules, immunized with the compound peptides, exhibited enhanced gp160-specific CTL activity.
摘要翻译： 由先前鉴定为在四种不同单倍型小鼠中诱导增殖反应的HIV的多重决定子T辅助肽和52-73％的HIV阳性患者（簇肽）中的IL-2应答组成的多肽构建体由co 与HIV-1 gp 160的V3环的肽18线性合成，对应于HIV-IIIB的主要中和决定簇，并且还显示含有显性CTL表位。 在先前对肽包含的T细胞表位作出反应的所有小鼠菌株中进行单次免疫后，引发了针对肽18抗体的协同帮助。 在两种小鼠中，达到的中和抗体水平与足以保护黑猩猩同源病毒攻击的水平相当。 在单次加强后，实现了在1:1000至1：16,000范围内高达90％中和的高得多的抗体滴度。 来自共享Dd I类MHC分子但具有用化合物肽免疫的不同II类分子的三种不同MHC单元型的小鼠的脾细胞表现出增强的gp160特异性CTL活性。
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公开(公告)号：US6039201A
公开(公告)日：2000-03-21
申请号：US938401
申请日：1997-09-26
申请人： James Kesterman , David Pendleton
发明人： James Kesterman , David Pendleton
CPC分类号： B67D7/3209
摘要： A container such as a sump having a bottom, at least one wall extending upwardly from the bottom, and a flange connected to the at least one wall and projecting therefrom. The flange preferably has apertures extending partially into the interior portion of the flange from its exterior surface. The flange provides structural rigidity to the container and prevents irregularities from being formed in the walls during manufacture. Preferably, the container is a sump having four lower walls, four upper walls integrally connected to the lower walls by the flange, and four entrance walls integrally connected to the upper walls and defining a mouth for receiving fluids. The flange preferably has an upper surface having upper apertures and a lower surface having lower apertures that are axially aligned with the upper apertures. Pins may utilized during manufacture of the sump by rotational molding to form apertures within the flange and aid in preventing irregularities from being formed.
摘要翻译： 诸如具有底部的容器，至少一个从底部向上延伸的壁的容器，以及连接到至少一个壁并从其突出的凸缘。 凸缘优选地具有从其外表面部分地延伸到凸缘的内部部分中的孔。 该凸缘为容器提供结构刚度，并防止在制造过程中在壁中形成凹凸。 优选地，容器是具有四个下壁的四个底壁，通过凸缘一体连接到下壁的四个上壁和与上壁一体地连接并限定用于接收流体的口的四个入口壁。 凸缘优选地具有上表面和上表面，下表面具有与上孔径轴向对准的下孔。 可以通过旋转模制在储槽的制造期间使用销来在凸缘内形成孔，并有助于防止形成凹凸。
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公开(公告)号：US5819975A
公开(公告)日：1998-10-13
申请号：US728257
申请日：1996-10-08
申请人： David Pendleton , James Kesterman
发明人： David Pendleton , James Kesterman
CPC分类号： B67D7/3209 , B67D7/78
摘要： A dispenser sump which includes a deflection surface and a distribution channel for distributing liquids about the interior periphery of the sump to allow insertion of fluid removal apparatus along any wall of the sump. The sump also provides increased structural rigidity to support backfill surrounding the sump and to maintain shape integrity.
摘要翻译： 一种分配器槽，包括偏转表面和分配通道，用于在贮槽的内周分布液体，以允许流体移除装置沿贮槽的任何壁插入。 油底壳还提供增加的结构刚性以支持围绕储槽的回填并保持形状完整性。
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公开(公告)号：US06685944B1
公开(公告)日：2004-02-03
申请号：US09763260
申请日：2001-10-19
IPC分类号： A61K3929
CPC分类号： C07K14/005 , A61K39/00 , A61K39/12 , A61K39/29 , A61K2039/53 , A61K2039/55522 , A61K2039/55538 , A61K2039/55566 , A61K2039/57 , C12N2710/24143 , C12N2770/24222 , C12N2770/24234
摘要： The present invention provides 1) an isolated peptide having the amino acid sequence DLMGYIPAV, (SEQ ID NO: 1); 2) an isolated HCV core polypeptide comprising an L→A substitution at amino acid position 139; 3) an isolated HCV core polypeptide having the amino acid sequence of SEQ ID NO: 2; and 4) a fragment of an HCV core polypeptide having fewer amino acids than the entire HCV core polypeptide and comprising the amino acid sequence SEQ ID NO: 1. Also provided are nucleic acids which encode the peptides and polypeptides of this invention, vectors comprising the nucleic acids of this invention and cells comprising the vectors and nucleic acids of this invention. The present invention further provides methods of producing an immune response in a subject and/or treating or preventing HCV infection in a subject, comprising administering to the subject, or to a cell of the subject, any of the compositions of this invention.
摘要翻译： 本发明提供1）具有氨基酸序列DLMGYIPAV（SEQ ID NO：1）的分离肽; 2）在氨基酸位置139包含L> A取代的分离的HCV核心多肽; 3）具有SEQ ID NO：2的氨基酸序列的分离的HCV核心多肽; 和4）具有比整个HCV核心多肽更少的氨基酸并且包含氨基酸序列SEQ ID NO：1的HCV核心多肽的片段。还提供编码本发明的肽和多肽的核酸，其包含 本发明的核酸和包含本发明的载体和核酸的细胞。 本发明进一步提供了在受试者中产生免疫应答和/或治疗或预防受试者的HCV感染的方法，其包括对受试者或受试者的细胞施用任何本发明的组合物。
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6.发明授权Potent peptide for stimulation of cytotoxic T lymphocytes specific for the HIV-1 envelope 失效用于刺激特异于HIV-1信封的细胞毒性T淋巴细胞的强力肽
公开(公告)号：US5976541A
公开(公告)日：1999-11-02
申请号：US847311
申请日：1992-03-06
申请人： Jay A. Berzofsky , Toshiyuki Taskeshita , Mutsunori Shirai , C. David Pendleton , Steven Kozlowski , David H. Margulies
发明人： Jay A. Berzofsky , Toshiyuki Taskeshita , Mutsunori Shirai , C. David Pendleton , Steven Kozlowski , David H. Margulies
IPC分类号： A61K38/03 , A61K38/55 , A61K39/00 , A61P31/18 , C07K7/06 , C07K7/08 , C07K14/16 , C12N15/48 , A61K39/21 , A61K39/12 , A61K39/38 , C12N15/00
CPC分类号： C07K14/005 , A61K38/03 , A61K38/55 , A61K38/556 , A61K39/00 , C12N2740/16122
摘要： Peptides having high activity in the eliciting of a cytotoxic T lymphocyte response to the HIV-1 envelope glycoprotein gpl60 are described. The activation of 12-15 residue peptides by proteolytic degradation to shorter peptides is shown as are general techniques for characterizing such activation processes.
摘要翻译： 描述了在引起对HIV-1包膜糖蛋白gp1660的细胞毒性T淋巴细胞反应中具有高活性的肽。 通过蛋白水解降解到较短肽的12-15个残基肽的活化显示为表征这种活化过程的一般技术。
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公开(公告)号：US5813797A
公开(公告)日：1998-09-29
申请号：US781337
申请日：1997-01-10
IPC分类号： B67D7/78 , B65D90/10 , B65D90/24 , B65G17/08 , B65G17/38 , B67D7/32 , B67D7/84 , E02D29/14 , B65B1/04 , E02B13/00
CPC分类号： B65D90/105 , B65D90/24 , B67D7/3209
摘要： A unitary sump frame made from a continuous material is disclosed. A substantially planar and continuous flange has an inner perimeter defining an opening in the frame and an outer perimeter. A lip is integrally connected to the flange and extends downwardly from the flange. The lip includes a securing mechanism that is operative to attach a component to the frame. A lug is integrally connected to the lip and extends away from the opening. The lug is operative for anchoring the frame.
摘要翻译： 公开了由连续材料制成的整体贮槽框架。 基本上平面和连续的凸缘具有限定框架中的开口和外周边的内周边。 唇缘一体地连接到凸缘并从凸缘向下延伸。 唇缘包括固定机构，其可操作以将部件附接到框架。 凸耳一体地连接到唇缘并远离开口延伸。 凸耳用于固定框架。
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公开(公告)号：US20050241723A1
公开(公告)日：2005-11-03
申请号：US10836978
申请日：2004-04-30
CPC分类号： F16L25/08 , B65D90/00 , B67D7/3209 , B67D7/78
摘要： Drop tube inserts and apparatus adapted for use with a riser pipe of a liquid reservoir are provided. Apparatus include a drop tube insert at least partially disposed within an end portion of a drop tube. The drop tube insert includes a wall with inner and outer surfaces wherein the wall of the drop tube insert is devoid of any opening extending between its inner and outer surfaces.
摘要翻译： 提供适于与液体储存器的提升管一起使用的落管插入件和设备。 装置包括至少部分地设置在滴管的端部内的落管插入件。 落管插入件包括具有内表面和外表面的壁，其中滴管插入件的壁没有在其内表面和外表面之间延伸的任何开口。
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9.发明授权Multideterminant peptides that elicit helper T-lymphocyte, cytotoxic T lymphocyte and neutralizing antibody responses against HIV-1 失效引起辅助性T淋巴细胞，细胞毒性T淋巴细胞和中和抗HIV-1抗体的多决定肽公开(公告)号：US06214347B1
公开(公告)日：2001-04-10
申请号：US08455685
申请日：1995-05-31
IPC分类号： A61K3921
CPC分类号： C07K14/005 , A61K38/03 , A61K38/55 , A61K38/556 , A61K39/00 , A61K39/12 , A61K39/21 , A61K2039/545 , A61K2039/55516 , A61K2039/55566 , A61K2039/55577 , A61K2039/57 , A61K2039/645 , C07K2319/00 , C12N2740/16122 , C12N2740/16134 , Y02A50/412 , A61K2300/00
摘要： The invention is directed to peptides of the HIV-1 envelope protein presenting multiple immune determinants. The peptide elicits both humoral and cell-mediated immune responses in mice having a variety of MHC types. In other embodiments, the invention is directed to immunogens composed of the peptides and methods for immunization employing them.
摘要翻译： 本发明涉及呈现多种免疫决定簇的HIV-1包膜蛋白的肽。 该肽在具有各种MHC类型的小鼠中引起体液和细胞介导的免疫应答。 在其它实施方案中，本发明涉及由肽组成的免疫原和使用它们的免疫方法。
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10.发明申请公开(公告)号：US20050241695A1
公开(公告)日：2005-11-03
申请号：US10836783
申请日：2004-04-30
CPC分类号： B65D90/26 , F16L25/06 , F16L25/08 , Y10T137/0491 , Y10T137/7475 , Y10T137/7485
摘要： Drop tube segments are provided. Each drop tube segment may be attached to another drop tube segment of a drop tube assembly. The drop tube segments include a wall with a fastener receiving structure that is devoid of any opening extending between inner and outer surfaces of the wall. Methods of making a drop tube assembly are also provided. The methods include the step of inserting a second end portion of a first conduit over a first end portion of a second conduit while edges of an aperture extend radially outwardly away from an outer surface of a first wall of the first conduit.
摘要翻译： 提供滴管段。 每个下落管段可以附接到下降管组件的另一个下落管段。 下降管段包括具有紧固件接收结构的壁，其没有在壁的内表面和外表面之间延伸的任何开口。 还提供了制造滴管组件的方法。 所述方法包括将第一导管的第二端部插入第二导管的第一端部的步骤，同时孔的边缘径向向外延伸远离第一导管的第一壁的外表面。
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