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    PROBES AND METHODS FOR DETECTION OF PATHOGENS AND ANTIBIOTIC RESISTANCE
    3.
    发明申请
    PROBES AND METHODS FOR DETECTION OF PATHOGENS AND ANTIBIOTIC RESISTANCE 审中-公开
    用于检测病原体和抗生素耐药性的探针和方法

    公开(公告)号：US20110027782A1

    公开(公告)日：2011-02-03

    申请号：US12788101

    申请日：2010-05-26

    申请人： DAVID A. HAAKE ,  BERNARD M. CHURCHILL ,  JOSEPH C. LIAO ,  MARC A. SUCHARD ,  YANG LI ,  MITRA MASTALI

    发明人： DAVID A. HAAKE ,  BERNARD M. CHURCHILL ,  JOSEPH C. LIAO ,  MARC A. SUCHARD ,  YANG LI ,  MITRA MASTALI

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/689

    摘要： Described are probes and methods for detecting pathogens and antibiotic resistance of a specimen. The method comprises contacting the specimen with a growth medium; and lysing the specimen to release nucleic acid molecules from the specimen. The lysate of the specimen is contacted with a capture probe immobilized on a substrate, wherein the capture probe comprises an oligonucleotide that specifically hybridizes with a first target nucleic acid sequence region of ribosomal RNA. The lysate is in contact with a detector probe that comprises a detectably labeled oligonucleotide that specifically hybridizes with a second target nucleic acid sequence region of ribosomal RNA. The presence or absence of labeled oligonucleotide complexed with the substrate is determined. Detection of labeled oligonucleotide complexed with the substrate is indicative of the presence of pathogen. Performing the method in the presence and absence of an antibiotic permits detection of antibiotic resistance.

    摘要翻译： 描述了用于检测样品的病原体和抗生素抗性的探针和方法。 该方法包括使样品与生长培养基接触; 并裂解样品以从样品中释放核酸分子。 将样品的裂解物与固定在底物上的捕获探针接触，其中捕获探针包含与核糖体RNA的第一靶核酸序列区特异性杂交的寡核苷酸。 裂解物与检测器探针接触，检测器探针包含与核糖体RNA的第二靶核酸序列区特异性杂交的可检测标记的寡核苷酸。 确定与底物复合的标记寡核苷酸的存在或不存在。 与底物复合的标记寡核苷酸的检测表明存在病原体。 在存在和不存在抗生素的情况下进行该方法可以检测抗生素耐药性。

    Probes and methods for detection of Escheridia coli and antibiotic resistance
    4.
    发明授权
    Probes and methods for detection of Escheridia coli and antibiotic resistance 有权
    用于检测大肠杆菌和抗生素耐药性的探针和方法

    公开(公告)号：US07763426B2

    公开(公告)日：2010-07-27

    申请号：US11743071

    申请日：2007-05-01

    申请人： David A. Haake ,  Bernard M. Churchill ,  Joseph C. Liao ,  Marc A. Suchard ,  Yang Li ,  Mitra Mastali

    发明人： David A. Haake ,  Bernard M. Churchill ,  Joseph C. Liao ,  Marc A. Suchard ,  Yang Li ,  Mitra Mastali

    IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12Q1/689

    摘要： Described are probes and methods for detecting pathogens and antibiotic resistance of a specimen. The method comprises contacting the specimen with a growth medium; and lysing the specimen to release nucleic acid molecules from the specimen. The lysate of the specimen is contacted with a capture probe immobilized on a substrate, wherein the capture probe comprises an oligonucleotide that specifically hybridizes with a first target nucleic acid sequence region of ribosomal RNA. The lysate is in contact with a detector probe that comprises a detectably labeled oligonucleotide that specifically hybridizes with a second target nucleic acid sequence region of ribosomal RNA. The presence or absence of labeled oligonucleotide complexed with the substrate is determined. Detection of labeled oligonucleotide complexed with the substrate is indicative of the presence of pathogen. Performing the method in the presence and absence of an antibiotic permits detection of antibiotic resistance.

    摘要翻译： 描述了用于检测样品的病原体和抗生素抗性的探针和方法。 该方法包括使样品与生长培养基接触; 并裂解样品以从样品中释放核酸分子。 将样品的裂解物与固定在底物上的捕获探针接触，其中捕获探针包含与核糖体RNA的第一靶核酸序列区特异性杂交的寡核苷酸。 裂解物与检测器探针接触，检测器探针包含与核糖体RNA的第二靶核酸序列区特异性杂交的可检测标记的寡核苷酸。 确定与底物复合的标记寡核苷酸的存在或不存在。 与底物复合的标记寡核苷酸的检测表明存在病原体。 在存在和不存在抗生素的情况下进行该方法可以检测抗生素耐药性。