公开(公告)号：US20150307850A1

公开(公告)日：2015-10-29

申请号：US14649669

申请日：2013-12-04

Applicant: Tong-ming FU ,  Dai WANG ,  Danilo CASIMIRO ,  Daniel FREED ,  Fengsheng LI ,  MERCK SHARP & DOHME CORP.

Inventor： Tong-Ming Fu ,  Dai Wang ,  Danilo Casimiro ,  Daniel C. Freed ,  Fengsheng Li

IPC: C12N7/00 ,  A61K39/245 ,  C07K14/005

CPC classification number: C12N7/00 ,  A61K39/12 ,  A61K39/21 ,  A61K39/245 ,  A61K2039/5256 ,  C07K14/005 ,  C12N15/86 ,  C12N2710/16121 ,  C12N2710/16122 ,  C12N2710/16134 ,  C12N2710/16141 ,  C12N2710/16152 ,  C12N2710/16162 ,  C12N2740/16234

Abstract: The present invention relates to a cytomegalovirus (CMV) which has been recombinantly altered to express a heterologous polypeptide and to allow for external control of viral replication. The heterologous polypeptide may be a polypeptide of interest such as an antigen, antibody or immune modulator. The CMV vectors of the invention are replication defective, or chemically controllable replication capable, or replication competent. The present invention also relates to uses of the CMV vectors such as inducing an immune response to an antigen or expressing an antibody or immune modulator in vivo. Compositions comprising the CMV expressing the heterologous polypeptide are also encompassed by the present invention.

Abstract translation: 本发明涉及重组改变以​​表达异源多肽并允许外部控制病毒复制的巨细胞病毒（CMV）。 异源多肽可以是感兴趣的多肽，例如抗原，抗体或免疫调节剂。 本发明的CMV载体是复制缺陷的，或具有化学上可控的复制能力或复制能力的。 本发明还涉及CMV载体的用途，例如在体内诱导对抗原的免疫应答或表达抗体或免疫调节剂。 包含表达异源多肽的CMV的组合物也包括在本发明中。

公开(公告)号：US20240018454A1

公开(公告)日：2024-01-18

申请号：US18038854

申请日：2021-11-23

Applicant: Daniel DELUBAC ,  Daniel NELSON ,  John CALAWAY ,  Daniel FREED ,  Xilis, Inc.

Inventor： Daniel DELUBAC ,  Daniel NELSON ,  John CALAWAY ,  Daniel FREED

IPC: C12M3/06 ,  C12M1/34 ,  B01L3/00

CPC classification number: C12M23/16 ,  C12M41/36 ,  C12M41/12 ,  B01L3/502761 ,  B01L2300/0867 ,  B01L2200/0652 ,  B01L2400/043

Abstract: Disclosed herein are systems, apparatuses, and methods for forming micro-organospheres. In some variations, a system may comprise a micro-organosphere generator configured to form a set of micro-organospheres from a mixture of a biological sample and a fluid. A controller may be coupled to an imaging device. The controller may be configured to receive the imaging data corresponding to one or more of the mixture or the set of micro-organospheres, and estimate one or more characteristics of the set of micro-organospheres based at least on the imaging data.

公开(公告)号：US20050271222A1

公开(公告)日：2005-12-08

申请号：US10912690

申请日：2004-08-04

Applicant: Daniel Freed ,  Sigfrid Soli

Inventor： Daniel Freed ,  Sigfrid Soli

IPC: H04B15/00 ,  H04R3/00 ,  H04R25/00

CPC classification number: H04R25/453 ,  H04R5/023

Abstract: A decorrelation method for improving feedback cancellation utilizes a small frequency shifting ratio, on the order of 0.3 percent. Frequency shifting is applied only to the high frequency portion of the signal, which is shifted alternately upward and downward.

Abstract translation: 用于改善反馈消除的去相关方法使用小的变频比，大约为0.3％。 只对信号的高频部分进行频移，上下交替地交替移动。