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    Thermostable alpha1-antitrypsin mutein with a disulfide bond and the method for preparing it
    3.
    发明授权
    Thermostable alpha1-antitrypsin mutein with a disulfide bond and the method for preparing it 有权
    具有二硫键的热稳定性α1-抗胰蛋白酶突变蛋白及其制备方法

    公开(公告)号：US08088738B2

    公开(公告)日：2012-01-03

    申请号：US12743920

    申请日：2007-11-22

    申请人： Myeong-Hee Yu ,  Je-Hyun Baek ,  Cheolju Lee

    发明人： Myeong-Hee Yu ,  Je-Hyun Baek ,  Cheolju Lee

    IPC分类号： A61K38/55 ,  C07K14/00

    CPC分类号： C07K14/8125

    摘要： The present invention relates to a thermostable alpha-1-antitrypsin mutein with a disulfide bond and a preparation method of the same, more precisely an alpha-1-antitrypsin mutein with improved thermostability, compared with the conventional thermostable alpha-1-antitrypsin mutein (Korean Patent No. 133252), resulted from the process of substituting the 168th and 189th amino acids of the alpha-1-antitrypsin with cysteines which are then linked by a disulfide bond, with keeping its alpha-1-antitrypsin activity unchanged, and a preparing method of the same. The thermostable alpha-1-antitrypsin mutein with a disulfide bond of the invention can be effectively used for a protein based therapeutic agent which is very stable in vivo, and can be applied further in various fields including in the development of a diagnostic reagent and in the preparation of an affinity column.

    摘要翻译： 本发明涉及具有二硫键的热稳定性α-1-抗胰蛋白酶突变蛋白及其制备方法，更准确地说，与常规的热稳定性α-1-抗胰蛋白酶突变蛋白相比，具有改善的热稳定性的α-1-抗胰蛋白酶突变蛋白 韩国专利133252）是由α-1-抗胰蛋白酶的第168位和第189位氨基酸用半胱氨酸取代的方法产生的，然后用二硫键连接，保持其α-1-抗胰蛋白酶活性不变， 制备方法相同。 具有本发明二硫键的热稳定性α-1-抗胰蛋白酶突变蛋白可有效地用于体内非常稳定的蛋白质治疗剂，并且可以进一步应用于各种领域，包括诊断试剂的开发和 亲和柱的制备。

    THERMOSTABLE ALPHA1-ANTITRYPSIN MUTEIN WITH A DISULFIDE BOND AND THE METHOD FOR PREPARING IT
    4.
    发明申请
    THERMOSTABLE ALPHA1-ANTITRYPSIN MUTEIN WITH A DISULFIDE BOND AND THE METHOD FOR PREPARING IT 有权
    具有破乳粘结剂的可热稳定的ALPHA1-抗菌素MUTEIN及其制备方法

    公开(公告)号：US20100248309A1

    公开(公告)日：2010-09-30

    申请号：US12743920

    申请日：2007-11-22

    申请人： Myeong-Hee Yu ,  Je-Hyun Baek ,  Cheolju Lee

    发明人： Myeong-Hee Yu ,  Je-Hyun Baek ,  Cheolju Lee

    IPC分类号： C07K14/435 ,  C07H21/04 ,  C12N15/63 ,  C12N1/21 ,  C12P21/00

    CPC分类号： C07K14/8125

    摘要： The present invention relates to a thermostable alpha-1-antitrypsin mutein with a disulfide bond and a preparation method of the same, more precisely an alpha-1-antitrypsin mutein with improved thermostability, compared with the conventional thermostable alpha-1-antitrypsin mutein (Korean Patent No. 133252), resulted from the process of substituting the 168th and 189th amino acids of the alpha-1-antitrypsin with cysteines which are then linked by a disulfide bond, with keeping its alpha-1-antitrypsin activity unchanged, and a preparing method of the same. The thermostable alpha-1-antitrypsin mutein with a disulfide bond of the invention can be effectively used for a protein based therapeutic agent which is very stable in vivo, and can be applied further in various fields including in the development of a diagnostic reagent and in the preparation of an affinity column.

    摘要翻译： 本发明涉及具有二硫键的热稳定性α-1-抗胰蛋白酶突变蛋白及其制备方法，更准确地说，与常规的热稳定性α-1-抗胰蛋白酶突变蛋白相比，具有改善的热稳定性的α-1-抗胰蛋白酶突变蛋白 韩国专利133252）是由α-1-抗胰蛋白酶的第168位和第189位氨基酸用半胱氨酸取代的方法产生的，然后用二硫键连接，保持其α-1-抗胰蛋白酶活性不变， 制备方法相同。 具有本发明二硫键的热稳定性α-1-抗胰蛋白酶突变蛋白可有效地用于体内非常稳定的蛋白质治疗剂，并且可以进一步应用于各种领域，包括诊断试剂的开发和 亲和柱的制备。