公开(公告)号：US11629381B2

公开(公告)日：2023-04-18

申请号：US16056254

申请日：2018-08-06

Applicant: BillionToOne, Inc.

Inventor： David Tsao ,  Sukrit Silas ,  Oguzhan Atay

IPC: C12Q1/6876 ,  C12Q1/6869 ,  G16B30/00 ,  G16B20/00 ,  G16B40/30 ,  G16B20/10 ,  G16H50/20 ,  C40B40/06 ,  C40B70/00

Abstract: Embodiments of a method and/or system can include generating a set of quality control template (QCT) molecules; determining a set of QCT sequence read clusters based on the set of QCT molecules, such as based on variation regions of the set of QCT molecules; and based on the set of QCT sequence read clusters, determining a sequencing-related parameter, such as a contamination parameter and/or molecule count parameter, associated with the at least one of sequencing library preparation and sequencing.

公开(公告)号：US20220010379A1

公开(公告)日：2022-01-13

申请号：US16923005

申请日：2020-07-07

Applicant: BillionToOne, Inc.

Inventor： Brian Landry ,  Alexander Ni

IPC: C12Q1/6883 ,  C12Q1/6869

Abstract: A highly accurate non-invasive prenatal testing methodology that allows accurate fetal rhesus determination and fetal DNA fraction measurement in a single assay is provided. The disclosed testing may be administered as early as at week 10 of pregnancy and can be conveniently combined with prenatal genetic disease testing and detection.

公开(公告)号：US20190095577A1

公开(公告)日：2019-03-28

申请号：US16056112

申请日：2018-08-06

Applicant: BillionToOne, Inc.

Inventor： David Tsao ,  Oguzhan Atay

IPC: C12Q1/6869

Abstract: Embodiments of a method and/or system can include generating a set of target-associated molecules (e.g., spike-in molecules) associated with one or more biological targets; generating one or more spike-in mixtures based on processing the set of target-associated molecules with one or more samples including the one or more biological targets; performing one or more Sanger sequencing operations on the one or more spike-in mixtures; determining one or more abundance metrics based on chromatogram-related outputs from the one or more Sanger sequencing operations; and/or facilitating characterization of one or more medical conditions based on the one or more abundance metrics.

公开(公告)号：US20240360519A1

公开(公告)日：2024-10-31

申请号：US18762467

申请日：2024-07-02

Applicant: BillionToOne, Inc.

Inventor： Patrick YE ,  David TSAO ,  Oguzhan ATAY

IPC: C12Q1/6886 ,  C12Q1/6806 ,  C12Q1/6869

CPC classification number: C12Q1/6886 ,  C12Q1/6869 ,  C12Q1/6806 ,  C12Q2600/112 ,  C12Q2600/154

Abstract: Methods to quantify methylation in a DNA sample include treating the sample to encode the presence or absence of DNA methylation, adding to the sample a set of synthetic molecules (e.g., quality control template (QCT) molecules), generating a co-amplification mixture, sequencing the co-amplification mixture, and determining a number of methylated molecules in the sample based on the number of methylated reads from the sample and a number of reads from the set of synthetic molecules.

公开(公告)号：US20240229140A1

公开(公告)日：2024-07-11

申请号：US18593639

申请日：2024-03-01

Applicant: BillionToOne, Inc.

Inventor： Brian LANDRY ,  Alexander NI

IPC: C12Q1/6883 ,  C12Q1/6869

CPC classification number: C12Q1/6883 ,  C12Q1/6869 ,  C12Q2600/156 ,  G01N2800/387

Abstract: A highly accurate non-invasive prenatal testing methodology that allows accurate fetal rhesus determination and fetal DNA fraction measurement in a single assay is provided. The disclosed testing may be administered as early as at week 10 of pregnancy and can be conveniently combined with prenatal genetic disease testing and detection.

公开(公告)号：US20220340971A1

公开(公告)日：2022-10-27

申请号：US17702708

申请日：2022-03-23

Applicant: BillionToOne, Inc.

Inventor： Devon Brian Chandler Brown ,  Anna Bueno ,  David Tsao ,  Oguzhan Atay

IPC: C12Q1/6883

Abstract: Aspects of the present disclosure include methods of detecting the presence or absence of one or more diseases using quantitative approaches. Aspects of the present disclosure include methods for determining the abundance of endogenous targets. Aspects of the present disclosure also include determining the presence or absence of an aneuploidy.

公开(公告)号：US20210292829A1

公开(公告)日：2021-09-23

申请号：US17210488

申请日：2021-03-23

Applicant: BillionToOne, Inc.

Inventor： Devon Brian Chandler Brown ,  Anna Bueno ,  David Tsao ,  Oguzhan Atay

IPC: C12Q1/6858 ,  C12Q1/6853 ,  C12Q1/686

Abstract: Aspects of the present disclosure include methods of detecting the presence or absence of one or more infectious diseases using quantitative approaches. In some aspects, the methods of the present disclosure include generating a spike-in mixture including target sample molecules (e.g., endogenous sample) and artificial molecules (e.g., spike in molecule, synthetic target-associated molecule), amplifying the spike in mixture to generate a co-amplified spike in mixture, and performing capillary electrophoresis to detect the presence or absence of one or more infectious diseases.

公开(公告)号：US20190114389A1

公开(公告)日：2019-04-18

申请号：US16055889

申请日：2018-08-06

Applicant: BillionToOne, Inc.

Inventor： David Tsao ,  Oguzhan Atay

IPC: G16B30/10 ,  G01N33/68

Abstract: Embodiments of a method and/or system for facilitating characterization of one or more conditions can include: generating a set of target-associated molecules; generating a reference-associated set of molecule; facilitating generation of at least one spike-in mixture; determining one or more abundance metrics based on an analysis of the at least one spike-in mixture; and facilitating the characterization of the one or more conditions based on the one or more abundance metrics.

公开(公告)号：US12176066B2

公开(公告)日：2024-12-24

申请号：US18189632

申请日：2023-03-24

Applicant: BillionToOne, Inc.

Inventor： David Tsao ,  Oguzhan Atay

IPC: G16B20/00 ,  C12N15/10 ,  C12Q1/68 ,  C12Q1/6827 ,  G01N33/68 ,  G16B5/00 ,  G16B25/00 ,  G16B30/00 ,  G16B50/00

Abstract: Embodiments of a method and/or system for facilitating characterization of one or more conditions can include: generating a set of target-associated molecules; generating a reference-associated set of molecule; facilitating generation of at least one spike-in mixture; determining one or more abundance metrics based on an analysis of the at least one spike-in mixture; and facilitating the characterization of the one or more conditions based on the one or more abundance metrics.

公开(公告)号：US20230295741A1

公开(公告)日：2023-09-21

申请号：US18187578

申请日：2023-03-21

Applicant: BillionToOne, Inc.

Inventor： Patrick YE ,  David TSAO ,  Oguzhan ATAY

IPC: C12Q1/6886 ,  C12Q1/6869

CPC classification number: C12Q1/6886 ,  C12Q1/6869 ,  C12Q2600/154 ,  C12Q1/6806

Abstract: Aspects of the present disclosure includes methods to quantify methylation in a DNA sample, where the method includes treating the sample to encode the presence or absence of DNA methylation, adding to the sample a set of synthetic molecules e.g., quality control template (QCT) molecules), generating a co-amplification mixture, sequencing the co-amplification mixture, determining a number of methylated molecules in the sample based on the number of methylated reads from the sample and a number of reads from the set of synthetic molecules.