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公开(公告)号:US20090203612A1
公开(公告)日:2009-08-13
申请号:US12422749
申请日:2009-04-13
申请人: Scott B. Bintrim , Jon C. Mitchell , Ignacio M. Larrinua , Patricia C. Apel-Birkhold , Susan B. Green , Barry W. Schafer , Scott A. Bevan , Scott A. Young , Lining Guo
发明人: Scott B. Bintrim , Jon C. Mitchell , Ignacio M. Larrinua , Patricia C. Apel-Birkhold , Susan B. Green , Barry W. Schafer , Scott A. Bevan , Scott A. Young , Lining Guo
CPC分类号: C12N15/8286 , Y02A40/162
摘要: The subject invention relates to novel Xenorhabdus toxin complex (TC) proteins and genes that encode these proteins. More specifically, the subject invention relates to TC genes and proteins obtainable from Xenorhabdus strain Xwi. The subject invention also provides an exochitinase obtainable from the Xwi strain. This exochitinase can be used to control insects using methods known in the art.
摘要翻译: 本发明涉及新型异戊酰梭菌毒素复合物(TC)蛋白质和编码这些蛋白质的基因。 更具体地,本发明涉及从Xenorhabdus菌株Xwi获得的TC基因和蛋白质。 本发明还提供可从Xwi菌株获得的外切蛋白酶。 该外切蛋白酶可以使用本领域已知的方法来控制昆虫。
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公开(公告)号:US06590142B1
公开(公告)日:2003-07-08
申请号:US09637048
申请日:2000-08-11
申请人: James K. Petell , Donald J. Merlo , Rod A. Herman , Jean L. Roberts , Lining Guo , Barry W. Schafer , Kitisri Sukhapinda , Ann Owens Merlo
发明人: James K. Petell , Donald J. Merlo , Rod A. Herman , Jean L. Roberts , Lining Guo , Barry W. Schafer , Kitisri Sukhapinda , Ann Owens Merlo
IPC分类号: A01H500
CPC分类号: C07K14/24 , C12N15/8286 , Y02A40/162
摘要: Novel polynucleotide sequences that encode insect toxins TcdA and TcbA have base compositions that differ substantially from the native genes, making them more similar to plant genes. The new sequences are suitable for use for high expression in both monocots and dicots. Transgenic plants with a genome comprising a nucleic acid of SEQ ID NO: 3 or SEQ ID NO:4 are insect resistant.
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公开(公告)号:US09170231B2
公开(公告)日:2015-10-27
申请号:US13877311
申请日:2011-08-22
CPC分类号: G01N27/62 , G01N30/463 , G01N30/7233 , G01N30/74 , G01N30/78 , G01N2030/884
摘要: Embodiments of the invention include methods of determining the allergen content of a composition. Embodiments of the invention may include providing a composition comprising an allergen; at least partially purifying the allergen from the composition to form an extract; and determining the amount of allergen in the extract using liquid chromatography with ultraviolet and mass spectrometric detection.
摘要翻译: 本发明的实施方案包括确定组合物的变应原含量的方法。 本发明的实施方案可以包括提供包含变应原的组合物; 至少部分地从组合物中纯化变应原以形成提取物; 并使用具有紫外和质谱检测的液相色谱法测定提取物中的变应原的量。
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公开(公告)号:US20100037358A1
公开(公告)日:2010-02-11
申请号:US12509806
申请日:2009-07-27
申请人: Barry W. Schafer , Rod A. Herman
发明人: Barry W. Schafer , Rod A. Herman
摘要: The invention provides a method for devitalizing plant seed, the method comprising the steps of hydrating a viable whole plant seed and freezing the hydrated whole plant seed. The invention further provides a collection of devitalized whole plant seed wherein the integrity of genomic DNA and protein within the devitalized plant seed is preserved.
摘要翻译: 本发明提供了一种使植物种子失活的方法,该方法包括以下步骤:将活的全植物种子进行水合并冷冻水合的全植物种子。 本发明还提供了一种失活的全植物种子的集合,其中保存了灭活的植物种子内的基因组DNA和蛋白质的完整性。
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公开(公告)号:US07517956B2
公开(公告)日:2009-04-14
申请号:US11375551
申请日:2006-03-14
申请人: Scott B. Bintrim , Jon C. Mitchell , Ignacio M. Larrinua , Patricia C. Apel-Birkhold , Susan B. Green , Barry W. Schafer , Scott A. Bevan , Scott A. Young , Lining Guo
发明人: Scott B. Bintrim , Jon C. Mitchell , Ignacio M. Larrinua , Patricia C. Apel-Birkhold , Susan B. Green , Barry W. Schafer , Scott A. Bevan , Scott A. Young , Lining Guo
CPC分类号: C12N15/8286 , Y02A40/162
摘要: The subject invention relates to novel Xenorhabdus toxin complex (TC) proteins and genes that encode these proteins. More specifically, the subject invention relates to TC genes and proteins obtainable from Xenorhabdus strain Xwi. The subject invention also provides an exochitinase obtainable from the Xwi strain. This exochitinase can be used to control insects using methods known in the art.
摘要翻译: 本发明涉及新型异戊酰梭菌毒素复合物(TC)蛋白质和编码这些蛋白质的基因。 更具体地,本发明涉及从Xenorhabdus菌株Xwi获得的TC基因和蛋白质。 本发明还提供可从Xwi菌株获得的外切蛋白酶。 该外切蛋白酶可以使用本领域已知的方法来控制昆虫。
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公开(公告)号:US20130280742A1
公开(公告)日:2013-10-24
申请号:US13877311
申请日:2011-08-22
IPC分类号: G01N27/62
CPC分类号: G01N27/62 , G01N30/463 , G01N30/7233 , G01N30/74 , G01N30/78 , G01N2030/884
摘要: Embodiments of the invention include methods of determining the allergen content of a composition. Embodiments of the invention may include providing a composition comprising an allergen; at least partially purifying the allergen from the composition to form an extract; and determining the amount of allergen in the extract using liquid chromatography with ultraviolet and mass spectrometric detection.
摘要翻译: 本发明的实施方案包括确定组合物的变应原含量的方法。 本发明的实施方案可以包括提供包含变应原的组合物; 至少部分地从组合物中纯化变应原以形成提取物; 并使用具有紫外和质谱检测的液相色谱法测定提取物中的变应原的量。
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公开(公告)号:US20120204294A1
公开(公告)日:2012-08-09
申请号:US13412474
申请日:2012-03-05
申请人: Barry W. Schafer , Rod A. Herman
发明人: Barry W. Schafer , Rod A. Herman
摘要: The invention provides a method for devitalizing plant seed, the method comprising the steps of hydrating a viable whole plant seed and freezing the hydrated whole plant seed. The invention further provides a collection of devitalized whole plant seed wherein the integrity of genomic DNA and protein within the devitalized plant seed is preserved.
摘要翻译: 本发明提供了一种使植物种子失活的方法,该方法包括以下步骤:将活的全植物种子进行水合并冷冻水合的全植物种子。 本发明还提供了一种失活的全植物种子的集合,其中保存了灭活的植物种子内的基因组DNA和蛋白质的完整性。
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公开(公告)号:US08153864B2
公开(公告)日:2012-04-10
申请号:US12509806
申请日:2009-07-27
申请人: Barry W. Schafer , Rod A. Herman
发明人: Barry W. Schafer , Rod A. Herman
摘要: The invention provides a method for devitalizing plant seed, the method comprising the steps of hydrating a viable whole plant seed and freezing the hydrated whole plant seed. The invention further provides a collection of devitalized whole plant seed wherein the integrity of genomic DNA and protein within the devitalized plant seed is preserved.
摘要翻译: 本发明提供了一种使植物种子失活的方法,该方法包括以下步骤:将活的全植物种子进行水合并冷冻水合的全植物种子。 本发明还提供了一种失活的全植物种子的集合,其中保存了灭活的植物种子内的基因组DNA和蛋白质的完整性。
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公开(公告)号:US07709623B2
公开(公告)日:2010-05-04
申请号:US12422749
申请日:2009-04-13
申请人: Scott B. Bintrim , Jon C. Mitchell , Ignacio M. Larrinua , Patricia C. Apel-Birkhold , Susan B. Green , Barry W. Schafer , Scott A. Bevan , Scott A. Young , Lining Guo
发明人: Scott B. Bintrim , Jon C. Mitchell , Ignacio M. Larrinua , Patricia C. Apel-Birkhold , Susan B. Green , Barry W. Schafer , Scott A. Bevan , Scott A. Young , Lining Guo
CPC分类号: C12N15/8286 , Y02A40/162
摘要: The subject invention relates to novel Xenorhabdus toxin complex (TC) proteins and genes that encode these proteins. More specifically, the subject invention relates to TC genes and proteins obtainable from Xenorhabdus strain Xwi. The subject invention also provides an exochitinase obtainable from the Xwi strain. This exochitinase can be used to control insects using methods known in the art.
摘要翻译: 本发明涉及新型异戊酰梭菌毒素复合物(TC)蛋白质和编码这些蛋白质的基因。 更具体地,本发明涉及从Xenorhabdus菌株Xwi获得的TC基因和蛋白质。 本发明还提供可从Xwi菌株获得的外切蛋白酶。 该外切蛋白酶可以使用本领域已知的方法来控制昆虫。
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公开(公告)号:US07071386B2
公开(公告)日:2006-07-04
申请号:US10753901
申请日:2004-01-07
申请人: Scott B. Bintrim , Jon C. Mitchell , Ignacio M. Larrinua , Patricia C. Apel-Birkhold , Susan B. Green , Barry W. Schafer , Scott A. Bevan , Scott A. Young , Lining Guo
发明人: Scott B. Bintrim , Jon C. Mitchell , Ignacio M. Larrinua , Patricia C. Apel-Birkhold , Susan B. Green , Barry W. Schafer , Scott A. Bevan , Scott A. Young , Lining Guo
CPC分类号: C12N15/8286 , Y02A40/162
摘要: The subject invention relates to novel nucleic acid encoding a Xenorhabdus strain Xwi toxin complex (TC) protein and plants and bacteria transformed therewith.
摘要翻译: 本发明涉及编码Xenorhabdus菌株Xwi毒素复合物(TC)蛋白质的新型核酸以及用其转化的植物和细菌。
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