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1.发明申请METHOD FOR THE PRODUCTION OF CONJUGATES OF INSULIN-LIKE GROWTH FACTOR-1 AND POLY(ETHYLENE GLYCOL) 审中-公开产生胰岛素样生长因子-1和聚（乙二醇）的方法公开(公告)号：US20100035817A1
公开(公告)日：2010-02-11
申请号：US12576266
申请日：2009-10-09
Applicant: Stephan Fischer , Friederike Hesse , Hendrik Knoetgen , Kurt Lang , Friedrich Metzger , Joerg Thomas Regula , Christian Schantz , Andreas Schaubmar , Hans-Joachim Schoenfeld
Inventor： Stephan Fischer , Friederike Hesse , Hendrik Knoetgen , Kurt Lang , Friedrich Metzger , Joerg Thomas Regula , Christian Schantz , Andreas Schaubmar , Hans-Joachim Schoenfeld
CPC classification number: C07K14/65 , A61K47/60 , C07K2319/21 , C07K2319/50
Abstract: The present invention relates to a fusion protein comprising IGF-I or an IGF-I variant N-terminally linked to the C-terminus of a propeptide. The invention relates also to a method involving the use of the aforementioned fusion protein in the production of a lysine-PEGylated IGF-I or IGF-I variant. The method comprises the steps of cultivating a prokaryotic host cell comprising an expression vector containing a nucleic acid encoding the fusion protein and causing the cell to express the fusion protein, recovering and PEGylating said fusion protein, cleaving said PEGylated fusion protein with IgA protease, and recovering lysine-PEGylated IGF-I or IGF-I variant. The invention relates also to a lysine-PEGylated IGF-I or IGF-I variant produced using the above method. In addition, the invention relates to a method for treating a neurodegenerative disorders like Alzheimer's Disease using the lysine-PEGylated IGF-I or IGF-I variant and a composition comprising the lysine-PEGylated IGF-I or IGF-I variant.
Abstract translation: 本发明涉及包含IGF-I或与前肽的C-末端N末端连接的IGF-1变体的融合蛋白。 本发明还涉及涉及在制备赖氨酸 - PEG化IGF-I或IGF-I变体中使用上述融合蛋白的方法。 该方法包括以下步骤：培养包含含有编码融合蛋白的核酸的表达载体的原核宿主细胞，并使细胞表达融合蛋白，回收聚乙二醇化所述融合蛋白，用IgA蛋白酶切割所述聚乙二醇化融合蛋白，以及 回收赖氨酸 - PEG化IGF-I或IGF-I变体。 本发明还涉及使用上述方法制备的赖氨酸 - 聚乙二醇化IGF-I或IGF-I变体。 此外，本发明涉及使用赖氨酸PEG化IGF-I或IGF-1变体和包含赖氨酸 - PEG化IGF-I或IGF-I变体的组合物治疗神经变性疾病如阿尔茨海默病的方法。
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公开(公告)号：US07531324B2
公开(公告)日：2009-05-12
申请号：US11531557
申请日：2006-09-13
Applicant: Phillip A. Patten , Sridhar Viswanathan , Torben Lauesgaard Nissen , Anne Vogt , Harald Kropshofer , Ralf Schumacher , Stephan Fischer , Stefan Seeber , Andreas Schaubmar , Roberto Falkenstein
Inventor： Phillip A. Patten , Sridhar Viswanathan , Torben Lauesgaard Nissen , Anne Vogt , Harald Kropshofer , Ralf Schumacher , Stephan Fischer , Stefan Seeber , Andreas Schaubmar , Roberto Falkenstein
CPC classification number: A61K38/212 , A61K47/60 , C07K14/56 , Y02A50/463
Abstract: The present invention provides interferon-alpha polypeptides and conjugates, and nucleic acids encoding the polypeptides. The invention also includes compositions comprising these polypeptides, conjugates, and nucleic acids; cells containing or expressing the polypeptides, conjugates, and nucleic acids; methods of making the polypeptides, conjugates, and nucleic acids; and methods of using the polypeptides, conjugates, and nucleic acids.
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公开(公告)号：US20050266465A1
公开(公告)日：2005-12-01
申请号：US11132722
申请日：2005-05-18
Applicant: Phillip Patten , Sridhar Viswanathan , Torben Nissen , Anne Vogt , Harald Kropshofer , Ralf Schumacher , Stephan Fischer , Stefan Seeber , Adelbert Grossmann , Friederike Hesse , Andreas Schaubmar , Roberto Falkenstein , Hans Koll , Markus Dembowski , Stephens Adam
Inventor： Phillip Patten , Sridhar Viswanathan , Torben Nissen , Anne Vogt , Harald Kropshofer , Ralf Schumacher , Stephan Fischer , Stefan Seeber , Adelbert Grossmann , Friederike Hesse , Andreas Schaubmar , Roberto Falkenstein , Hans Koll , Markus Dembowski , Stephens Adam
CPC classification number: A61K38/212 , A61K47/60 , C07K14/56 , Y02A50/463
Abstract: The present invention provides interferon-alpha polypeptides and conjugates, and nucleic acids encoding the polypeptides. The invention also includes compositions comprising these polypeptides, conjugates, and nucleic acids; cells containing or expressing the polypeptides, conjugates, and nucleic acids; methods of making the polypeptides, conjugates, and nucleic acids; and methods of using the polypeptides, conjugates, and nucleic acids.
Abstract translation: 本发明提供了干扰素-α多肽和缀合物，以及编码多肽的核酸。 本发明还包括包含这些多肽，缀合物和核酸的组合物; 含有或表达多肽，缀合物和核酸的细胞; 制备多肽，缀合物和核酸的方法; 以及使用多肽，缀合物和核酸的方法。
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4.发明授权公开(公告)号：US08476232B2
公开(公告)日：2013-07-02
申请号：US13590549
申请日：2012-08-21
Applicant: Stephan Fischer , Friederike Hesse , Hendrik Knoetgen , Kurt Lang , Friedrich Metzger , Joerg Thomas Regula , Christian Schantz , Andreas Schaubmar , Hans-Joachim Schoenfeld
Inventor： Stephan Fischer , Friederike Hesse , Hendrik Knoetgen , Kurt Lang , Friedrich Metzger , Joerg Thomas Regula , Christian Schantz , Andreas Schaubmar , Hans-Joachim Schoenfeld
CPC classification number: C07K14/65 , A61K47/60 , C07K2319/21 , C07K2319/50
Abstract: The present invention relates to a fusion protein comprising IGF-I or an IGF-I variant N-terminally linked to the C-terminus of a propeptide. The invention relates also to a method involving the use of the aforementioned fusion protein in the production of a lysine-PEGylated IGF-I or IGF-I variant. The method comprises the steps of cultivating a prokaryotic host cell comprising an expression vector containing a nucleic acid encoding the fusion protein and causing the cell to express the fusion protein, recovering and PEGylating said fusion protein, cleaving said PEGylated fusion protein with IgA protease, and recovering lysine-PEGylated IGF-I or IGF-I variant. The invention relates also to a lysine-PEGylated IGF-I or IGF-I variant produced using the above method. In addition, the invention relates to a method for treating a neurodegenerative disorders like Alzheimer's Disease using the lysine-PEGylated IGF-I or IGF-I variant and a composition comprising the lysine-PEGylated IGF-I or IGF-I variant.
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5.发明申请公开(公告)号：US20110009317A1
公开(公告)日：2011-01-13
申请号：US12791904
申请日：2010-06-02
Applicant: Beat Amrein , Stefan Foser , Kurt Long , Friedrich Metzger , Joerg Regula , Andreas Schaubmar , Friederike Hesse , Klaus-Peter Kuenkele , Martin Lanzendoerfer
Inventor： Beat Amrein , Stefan Foser , Kurt Long , Friedrich Metzger , Joerg Regula , Andreas Schaubmar , Friederike Hesse , Klaus-Peter Kuenkele , Martin Lanzendoerfer
CPC classification number: A61K47/60 , C07K14/475 , C07K14/65
Abstract: A conjugate consisting of an insulin-like growth factor-1 (IGF-I) variant and one or two poly(ethylene glycol) group(s), characterized in that said IGF-I variant has an amino acid alteration at up to three amino acid positions 27, 37, 65, 68 of the wild-type IGF-I amino acid sequence so that one or two of said amino acids is/are lysine and amino acid 27 is a polar amino acid but not lysine, is conjugated via the primary amino group(s) of said lysine(s) and said poly(ethylene glycol) group(s) have an overall molecular weight of from 20 to 100 kDa is disclosed. This conjugate is useful for the treatment of neurodegenerative disorders like Alzheimer's Disease.
Abstract translation: 由胰岛素样生长因子-1（IGF-I）变体和一个或两个聚（乙二醇）基团组成的缀合物，其特征在于所述IGF-1变体在至多三个氨基酸处具有氨基酸改变 野生型IGF-I氨基酸序列的酸性位置27,37,65,68，使得一个或两个所述氨基酸是赖氨酸，氨基酸27是极性氨基酸而不是赖氨酸，通过 公开了所述赖氨酸和所述聚（乙二醇）基团的伯氨基的总分子量为20至100kDa。 该缀合物可用于治疗神经变性疾病如阿尔茨海默病。
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Patent Agency Ranking
6.发明授权Method for the production of conjugates of insulin-like growth factor-1 and poly(ethylene glycol) 有权生产胰岛素样生长因子-1和聚（乙二醇）共轭物的方法公开(公告)号：US07625996B2
公开(公告)日：2009-12-01
申请号：US11846857
申请日：2007-08-29
Applicant: Stephan Fischer , Friederike Hesse , Hendrik Knoetgen , Kurt Lang , Friedrich Metzger , Joerg Thomas Regula , Christian Schantz , Andreas Schaubmar , Hans-Joachim Schoenfeld
Inventor： Stephan Fischer , Friederike Hesse , Hendrik Knoetgen , Kurt Lang , Friedrich Metzger , Joerg Thomas Regula , Christian Schantz , Andreas Schaubmar , Hans-Joachim Schoenfeld
CPC classification number: C07K14/65 , A61K47/60 , C07K2319/21 , C07K2319/50
Abstract: The present invention relates to a fusion protein comprising IGF-I or an IGF-I variant N-terminally linked to the C-terminus of a propeptide. The invention relates also to a method involving the use of the aforementioned fusion protein in the production of a lysine-PEGylated IGF-I or IGF-I variant. The method comprises the steps of cultivating a prokaryotic host cell comprising an expression vector containing a nucleic acid encoding the fusion protein and causing the cell to express the fusion protein, recovering and PEGylating said fusion protein, cleaving said PEGylated fusion protein with IgA protease, and recovering lysine-PEGylated IGF-I or IGF-I variant. The invention relates also to a lysine-PEGylated IGF-I or IGF-I variant produced using the above method. In addition, the invention relates to a method for treating a neurodegenerative disorders like Alzheimer's Disease using the lysine-PEGylated IGF-I or IGF-I variant and a composition comprising the lysine-PEGylated IGF-I or IGF-I variant.
Abstract translation: 本发明涉及包含IGF-I或与前肽的C-末端N末端连接的IGF-1变体的融合蛋白。 本发明还涉及涉及在制备赖氨酸 - PEG化IGF-I或IGF-I变体中使用上述融合蛋白的方法。 该方法包括以下步骤：培养包含含有编码融合蛋白的核酸的表达载体的原核宿主细胞，并使细胞表达融合蛋白，回收聚乙二醇化所述融合蛋白，用IgA蛋白酶切割所述聚乙二醇化融合蛋白，以及 回收赖氨酸 - PEG化IGF-I或IGF-I变体。 本发明还涉及使用上述方法制备的赖氨酸 - 聚乙二醇化IGF-I或IGF-I变体。 此外，本发明涉及使用赖氨酸PEG化IGF-I或IGF-1变体和包含赖氨酸 - PEG化IGF-I或IGF-I变体的组合物治疗神经变性疾病如阿尔茨海默病的方法。
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公开(公告)号：US20070025966A1
公开(公告)日：2007-02-01
申请号：US11531557
申请日：2006-09-13
Applicant: Phillip Patten , Sridhar Viswanathan , Torben Nissen , Anne Vogt , Harald Kropshofer , Ralf Schumacher , Stephan Fischer , Stefan Seeber , Adelbert Grossmann , Friederike Hesse , Andreas Schaubmar , Roberto Falkenstein , Hans Koll , Markus Dembowski
Inventor： Phillip Patten , Sridhar Viswanathan , Torben Nissen , Anne Vogt , Harald Kropshofer , Ralf Schumacher , Stephan Fischer , Stefan Seeber , Adelbert Grossmann , Friederike Hesse , Andreas Schaubmar , Roberto Falkenstein , Hans Koll , Markus Dembowski
CPC classification number: A61K38/212 , A61K47/60 , C07K14/56 , Y02A50/463
Abstract: The present invention provides interferon-alpha polypeptides and conjugates, and nucleic acids encoding the polypeptides. The invention also includes compositions comprising these polypeptides, conjugates, and nucleic acids; cells containing or expressing the polypeptides, conjugates, and nucleic acids; methods of making the polypeptides, conjugates, and nucleic acids; and methods of using the polypeptides, conjugates, and nucleic acids.
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8.发明授权Antibodies against amyloid beta 4 with glycosylation in the variable region 有权在可变区域中具有糖基化的淀粉样蛋白β4的抗体公开(公告)号：US08906370B2
公开(公告)日：2014-12-09
申请号：US12086309
申请日：2006-12-11
Applicant: Hansruedi Loetscher , Walter Huber , Diana Schuhbauer , Karl Weyer , Manfred Brockhaus , Bernd Bohrmann , Hans Koll , Andreas Schaubmar , Kurt Lang
Inventor： Hansruedi Loetscher , Walter Huber , Diana Schuhbauer , Karl Weyer , Manfred Brockhaus , Bernd Bohrmann , Hans Koll , Andreas Schaubmar , Kurt Lang
IPC: A61K39/395 , C07K16/18 , A61P25/28 , A61K39/00
CPC classification number: A61K39/3955 , A61K2039/505 , C07K16/18 , C07K2317/41 , C07K2317/56 , C07K2317/77
Abstract: The present invention relates to a purified antibody molecule preparation being characterized in that at least one antigen binding site comprises a glycosylated asparagine (Asn) in the variable region of the heavy chain (VH). More specifically, a pharmaceutical and a diagnostic composition comprising said antibody molecule and antibody mixtures are provided which is/are capable of specifically recognizing the β-A4 peptide/Aβ4. The present invention relates in particular to a mixture of antibodies comprising one or two glycosylated antigen binding sites with a glycosylated asparagine (Asn) in the variable region of the heavy chain, i.e. mixtures of isoforms of antibodies which comprise a glycosylated Asn in the variable region of the heavy chain (VH). Also disclosed are compositions or antibody preparations comprising the specifically glycosylated antibody isoforms. Furthermore, the pharmaceutical and diagnostic uses for these antibodies are provided. The antibody isoforms may for example be used in the pharmaceutical intervention of amyloidogenesis or amyloid-plaque formation and/or in the diagnosis of the same.
Abstract translation: 本发明涉及纯化的抗体分子制剂，其特征在于至少一个抗原结合位点包含重链可变区（VH）中的糖基化天冬酰胺（Asn）。 更具体地，提供了包含所述抗体分子和抗体混合物的药物和诊断组合物，其能够特异性识别Aβ-4肽/ A＆bgr; 4。 本发明特别涉及包含一个或两个糖基化抗原结合位点与重链可变区中的糖基化天冬酰胺（Asn）的抗体的混合物，即包含可变区中的糖基化Asn的抗体同种型的混合物 的重链（VH）。 还公开了包含特异性糖基化抗体同种型的组合物或抗体制剂。 此外，提供了这些抗体的药物和诊断用途。 抗体同种型可以例如用于淀粉样变形或淀粉样蛋白斑形成的药物干预和/或其诊断。
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9.发明申请METHOD FOR THE PRODUCTION OF CONJUGATES OF INSULIN-LIKE GROWTH FACTOR-1 AND POLY(ETHYLENE GLYCOL) 有权产生胰岛素样生长因子-1和聚（乙二醇）的方法公开(公告)号：US20130065831A1
公开(公告)日：2013-03-14
申请号：US13590549
申请日：2012-08-21
Applicant: Stephan Fischer , Friederike Hesse , Hendrik Knoetgen , Kurt Lang , Friedrich Metzger , Joerg Thomas Regula , Christian Schantz , Andreas Schaubmar , Hans-Joachim Schoenfeld
Inventor： Stephan Fischer , Friederike Hesse , Hendrik Knoetgen , Kurt Lang , Friedrich Metzger , Joerg Thomas Regula , Christian Schantz , Andreas Schaubmar , Hans-Joachim Schoenfeld
CPC classification number: C07K14/65 , A61K47/60 , C07K2319/21 , C07K2319/50
Abstract: The present invention relates to a fusion protein comprising IGF-I or an IGF-I variant N-terminally linked to the C-terminus of a propeptide. The invention relates also to a method involving the use of the aforementioned fusion protein in the production of a lysine-PEGylated IGF-I or IGF-I variant. The method comprises the steps of cultivating a prokaryotic host cell comprising an expression vector containing a nucleic acid encoding the fusion protein and causing the cell to express the fusion protein, recovering and PEGylating said fusion protein, cleaving said PEGylated fusion protein with IgA protease, and recovering lysine-PEGylated IGF-I or IGF-I variant. The invention relates also to a lysine-PEGylated IGF-I or IGF-I variant produced using the above method. In addition, the invention relates to a method for treating a neurodegenerative disorders like Alzheimer's Disease using the lysine-PEGylated IGF-I or IGF-I variant and a composition comprising the lysine-PEGylated IGF-I or IGF-I variant.
Abstract translation: 本发明涉及包含IGF-I或与前肽的C-末端N末端连接的IGF-1变体的融合蛋白。 本发明还涉及涉及在制备赖氨酸 - PEG化IGF-I或IGF-I变体中使用上述融合蛋白的方法。 该方法包括以下步骤：培养包含含有编码融合蛋白的核酸的表达载体的原核宿主细胞，并使细胞表达融合蛋白，回收聚乙二醇化所述融合蛋白，用IgA蛋白酶切割所述聚乙二醇化融合蛋白;以及 回收赖氨酸 - PEG化IGF-I或IGF-I变体。 本发明还涉及使用上述方法制备的赖氨酸 - 聚乙二醇化IGF-I或IGF-I变体。 此外，本发明涉及使用赖氨酸PEG化IGF-I或IGF-1变体和包含赖氨酸 - PEG化IGF-I或IGF-I变体的组合物治疗神经变性疾病如阿尔茨海默病的方法。
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公开(公告)号：US20110045510A1
公开(公告)日：2011-02-24
申请号：US12934678
申请日：2009-03-31
Applicant: Kurt Lang , Andreas Schaubmar , Julia Schleypen , Tilman Schlothauer
Inventor： Kurt Lang , Andreas Schaubmar , Julia Schleypen , Tilman Schlothauer
IPC: G01N33/543 , G01N33/566
CPC classification number: G01N33/566 , G01N33/74 , G01N2333/65
Abstract: The current invention reports an immunoassay for the determination of PEGylated insulin-like-growth-factor employing an anti-(polyethylene glycol) antibody and an anti-digoxygenin antibody for the detection of an insulin-like-growth-factor/insulin-like-growth-factor-binding-protein-complex.
Abstract translation: 本发明报道了使用抗（聚乙二醇）抗体和抗 - 地高辛素抗体测定胰岛素样生长因子/胰岛素样生长因子的聚乙二醇化胰岛素样生长因子的免疫测定， 生长因子结合蛋白复合物。
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