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公开(公告)号：US09284552B2
公开(公告)日：2016-03-15
申请号：US13854995
申请日：2013-04-02
Applicant: Katie Jansen Spayd , Jon Karpilow , John K. Wakefield
Inventor： Katie Jansen Spayd , Jon Karpilow , John K. Wakefield
IPC: C12N7/01 , C12N7/02 , C12N15/86 , A61K39/21 , C12N15/113
CPC classification number: C12N15/113 , C12N15/86 , C12N2740/15051 , C12N2740/15052
Abstract: The present disclosure provides a method of modifying cells in order to enhance lentiviral titers, cell lines that are modified and modifying reagents. By mediating individual genes and combination thereof, lentiviral titers may be increased.
Abstract translation: 本公开提供了一种修饰细胞以增强慢病毒滴度，经修饰和修饰试剂的细胞系的方法。 通过介导个体基因及其组合，可以增加慢病毒滴度。
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2.发明授权Chromophoric silyl protecting groups and their use in the chemical synthesis of oligonucleotides 有权发色体甲硅烷基保护基及其在寡核苷酸化学合成中的应用公开(公告)号：US08759508B2
公开(公告)日：2014-06-24
申请号：US12600829
申请日：2008-05-16
Applicant: Michael Oren Delaney
Inventor： Michael Oren Delaney
Abstract: The compounds are of class of chromophoric 1,2,3-triazolyl equipped silyl linking groups that are useful in the chemical synthesis of RNA. An example of a nucleoside comprising this group is
Abstract translation: 这些化合物是具有发色团的1,2,3-三唑基类的，可用于化学合成RNA的甲硅烷基连接基团。 包含该组的核苷的实例是
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3.发明申请siRNA Targeting Cyclin-dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) 审中-公开siRNA靶向细胞周期蛋白依赖性激酶抑制剂1B（p27，Kip1）（CDKN1B）公开(公告)号：US20130225667A1
公开(公告)日：2013-08-29
申请号：US13867168
申请日：2013-04-22
Applicant: Anastasia Khvorova , Angela Reynolds , Devin Leake , William Marshall , Steven Read , Stephen Scaringe
Inventor： Anastasia Khvorova , Angela Reynolds , Devin Leake , William Marshall , Steven Read , Stephen Scaringe
IPC: C07H21/02
CPC classification number: C07H21/02 , A61K31/713 , C07H21/00 , C12N15/111 , C12N15/113 , C12N15/1135 , C12N15/1136 , C12N15/1137 , C12N15/1138 , C12N2310/14 , C12N2320/10 , C12N2320/11 , C12N2330/30 , C12Y304/21047 , G16B20/00
Abstract: Efficient sequence specific gene silencing is possible through the use of siRNA technology. By selecting particular siRNAs by rational design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes. Methods, compositions, and kits generated through rational design of siRNAs are disclosed including those directed to nucleotide sequences for CDKN1B.
Abstract translation: 通过使用siRNA技术，有效的序列特异性基因沉默是可能的。 通过合理设计选择特定的siRNA，可以最大限度地产生有效的基因沉默试剂，以及沉默基因的方法。 公开了通过合理设计siRNA产生的方法，组合物和试剂盒，包括针对CDKN1B的核苷酸序列的那些。
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公开(公告)号：US20130217121A1
公开(公告)日：2013-08-22
申请号：US13854995
申请日：2013-04-02
Applicant: Katie Jansen Spayd , Jon Karpilow , John K. Wakefield
Inventor： Katie Jansen Spayd , Jon Karpilow , John K. Wakefield
IPC: C12N15/113
CPC classification number: C12N15/113 , C12N15/86 , C12N2740/15051 , C12N2740/15052
Abstract: The present disclosure provides a method of modifying cells in order to enhance lentiviral titers, cell lines that are modified and modifying reagents. By mediating individual genes and combination thereof, lentiviral titers may be increased.
Abstract translation: 本公开提供了一种修饰细胞以增强慢病毒滴度，经修饰和修饰试剂的细胞系的方法。 通过介导个体基因及其组合，可以增加慢病毒滴度。
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5.发明授权Duplex oligonucleotide complexes and methods for gene silencing by RNA interference 有权双链寡核苷酸复合物和通过RNA干扰进行基因沉默的方法公开(公告)号：US08501706B2
公开(公告)日：2013-08-06
申请号：US13552709
申请日：2012-07-19
Applicant: Christina Yamada , Anastasia Khvorova , Rob Kaiser , Emily Anderson , Devin Leake
Inventor： Christina Yamada , Anastasia Khvorova , Rob Kaiser , Emily Anderson , Devin Leake
IPC: C12N15/11
CPC classification number: C12N15/111 , C07H19/00 , C12N15/11 , C12N15/113 , C12N2310/14 , C12N2310/315 , C12N2310/321 , C12N2310/322 , C12N2310/346 , C12N2310/3515 , C12N2320/32 , C12N2330/30 , C12N2310/3521
Abstract: Provided herein are duplex oligonucleotide complexes which can be administered to a cell, tissue or organism to silence a target gene without the aid of a transfection reagent(s). The duplex oligonucleotide complexes of the disclosure include a conjugate moiety that facilitates delivery to a cell, tissue or organism.
Abstract translation: 本文提供了可以在不借助转染试剂的情况下向细胞，组织或生物体施用以沉默靶基因的双链寡核苷酸复合物。 本公开的双链寡核苷酸复合物包括有助于递送至细胞，组织或生物体的缀合物部分。
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Patent Agency Ranking
公开(公告)号：US08367318B2
公开(公告)日：2013-02-05
申请号：US12670363
申请日：2008-07-21
Applicant: Anja Smith , Scott Baskerville , Devin Leake , Annaleen Vermeulen , Barbara Robertson , Anastasia Khvorova
Inventor： Anja Smith , Scott Baskerville , Devin Leake , Annaleen Vermeulen , Barbara Robertson , Anastasia Khvorova
IPC: C12Q1/68
CPC classification number: C12N15/111 , C12N2310/113 , C12N2310/141 , C12N2320/12 , C12N2330/10 , C12Q1/6886 , C12Q2600/178
Abstract: The disclosure provides methods for inhibiting the activity of a miRNA cluster in a cell, and also for screening a cell for a phenotype(s) of interest resulting from inhibition of a miRNA cluster. The methods use a cluster pool which comprises at least one miRNA inhibitor specific for each miRNA in the miRNA cluster. MiRNA inhibitors are described that induce apoptosis in breast cancer cells and hence are useful in the treatment of breast cancer. The disclosure also provides pharmaceutical compositions which are useful for the treatment of breast cancer; methods for inducing the nuclear translocation of NF-κB in a breast cancer cell; methods for inducing the nuclear translocation of c-Jun in a breast cancer cell; method for inhibiting the nuclear translocation of NF-κB in a breast cancer cell; and methods for providing prognostic medical information relating to breast cancer progression.
Abstract translation: 本公开提供了抑制细胞中miRNA簇的活性的方法，以及用于筛选由miRNA群抑制引起的表型的细胞。 该方法使用聚类池，其包含至少一种对miRNA簇中的每个miRNA特异的miRNA抑制剂。 描述了诱导乳腺癌细胞凋亡的MiRNA抑制剂，因此可用于治疗乳腺癌。 本公开还提供了可用于治疗乳腺癌的药物组合物; 在乳腺癌细胞中诱导NF-κB的核易位的方法; 在乳腺癌细胞中诱导c-Jun的核易位的方法; 抑制乳腺癌细胞中NF-κB的核易位的方法; 以及用于提供与乳腺癌进展相关的预后医学信息的方法。
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7.发明授权公开(公告)号：US08247169B2
公开(公告)日：2012-08-21
申请号：US12660582
申请日：2010-03-01
Applicant: Anastasia Khvorova , Angela Reynolds , Devin Leake , William Marshall , Steven Read , Stephen Scaringe
Inventor： Anastasia Khvorova , Angela Reynolds , Devin Leake , William Marshall , Steven Read , Stephen Scaringe
CPC classification number: C12N15/113 , C12N15/111 , C12N15/1135 , C12N2310/11 , C12N2310/14 , C12N2320/11 , G06F19/18
Abstract: Efficient sequence specific gene silencing is possible through the use of siRNA technology. By selecting particular siRNAs by rational design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes. Methods, compositions, and kits generated through rational design of siRNAs are disclosed including those directed to nucleotide sequences for DGAT2.
Abstract translation: 通过使用siRNA技术，有效的序列特异性基因沉默是可能的。 通过合理设计选择特定的siRNA，可以最大限度地产生有效的基因沉默试剂，以及沉默基因的方法。 公开了通过合理设计siRNA产生的方法，组合物和试剂盒，包括针对DGAT2核苷酸序列的那些。
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公开(公告)号：US08198427B1
公开(公告)日：2012-06-12
申请号：US11980210
申请日：2007-10-30
Applicant: Anastasia Khvorova , Angela Reynolds , Devin Leake , William Marshall , Steven Read , Stephen Scaringe
Inventor： Anastasia Khvorova , Angela Reynolds , Devin Leake , William Marshall , Steven Read , Stephen Scaringe
CPC classification number: A61K31/713 , C07K14/705 , C12N9/90 , C12N15/111 , C12N15/113 , C12N15/1135 , C12N15/1136 , C12N15/1137 , C12N15/1138 , C12N2310/14 , C12N2320/11 , C12Y304/21047 , C12Y502/01008 , G06F19/18
Abstract: Efficient sequence specific gene silencing is possible through the use of siRNA technology. By selecting particular siRNAs by rational design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes. Methods, compositions, and kits generated through rational design of siRNAs are disclosed including those directed to nucleotide sequences for CTNNB1.
Abstract translation: 通过使用siRNA技术，有效的序列特异性基因沉默是可能的。 通过合理设计选择特定的siRNA，可以最大限度地产生有效的基因沉默试剂，以及沉默基因的方法。 公开了通过合理设计siRNA产生的方法，组合物和试剂盒，包括针对CTNNB1的核苷酸序列的那些。
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9.发明申请公开(公告)号：US20120052487A9
公开(公告)日：2012-03-01
申请号：US10940892
申请日：2004-09-14
Applicant: Anastasia Khvorova , Angela Reynolds , Devin Leake , William Marshall , Steven Read , Stephen Scaringe
Inventor： Anastasia Khvorova , Angela Reynolds , Devin Leake , William Marshall , Steven Read , Stephen Scaringe
CPC classification number: C12Y113/12007 , A61K31/713 , C12N15/1048 , C12N15/111 , C12N15/113 , C12N15/1135 , C12N15/1136 , C12N15/1137 , C12N15/1138 , C12N2310/14 , C12N2320/10 , C12N2320/11 , C12Y502/01008 , G06F19/18
Abstract: Efficient sequence specific gene silencing is possible through the use of siRNA technology. By selecting particular siRNAs by rational design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes. Methods, compositions, and kits generated through rational design of siRNAs are disclosed.
Abstract translation: 通过使用siRNA技术，有效的序列特异性基因沉默是可能的。 通过合理设计选择特定的siRNA，可以最大限度地产生有效的基因沉默试剂，以及沉默基因的方法。 公开了通过合理设计siRNA产生的方法，组合物和试剂盒。
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公开(公告)号：US20120015850A1
公开(公告)日：2012-01-19
申请号：US13199946
申请日：2011-09-13
Applicant: Anastasia Khvorova , Angela Reynolds , Devin Leake , William Marshall , Steven Read , Stephen Scaringe
Inventor： Anastasia Khvorova , Angela Reynolds , Devin Leake , William Marshall , Steven Read , Stephen Scaringe
CPC classification number: C12N15/113 , C12N15/111 , C12N15/1135 , C12N2310/11 , C12N2310/14 , C12N2320/11 , G16B20/00
Abstract: Efficient sequence specific gene silencing is possible through the use of siRNA technology. By selecting particular siRNAs by rational design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes. Methods, compositions, and kits generated through rational design of siRNAs are disclosed including those directed to nucleotide sequences for SOD1.
Abstract translation: 通过使用siRNA技术，有效的序列特异性基因沉默是可能的。 通过合理设计选择特定的siRNA，可以最大限度地产生有效的基因沉默试剂，以及沉默基因的方法。 公开了通过合理设计siRNA产生的方法，组合物和试剂盒，包括针对SOD1的核苷酸序列的那些。
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