公开(公告)号：US4684609A

公开(公告)日：1987-08-04

申请号：US273813

申请日：1981-06-15

申请人： Su-Ming Hsu

发明人： Su-Ming Hsu

IPC分类号： G01N33/58 ,  G01N33/54 ,  C12N9/96

CPC分类号： G01N33/581 ,  Y10S435/81 ,  Y10S435/96 ,  Y10S435/962 ,  Y10S435/975

摘要： An enhanced labelling complex for localizing markers on a prepared tissue section is disclosed. The complex includes both avidin components and biotinylated macromolecular components at a ratio preselected to provide a complex which is sufficiently large to include a large number of labels, and sufficiently small to penetrate the tissue section. The markers are localized by first introducing a biotinylated link into the tissue section and thereafter exposing the section to the labelling complex.

摘要翻译： 公开了用于在制备的组织切片上定位标记物的增强的标记复合物。 复合物包括抗生物素蛋白组分和生物素化大分子组分，其预选比例提供了足够大的复合物，其包含大量标记，足够小以渗透组织切片。 通过首先将生物素化链接引入组织切片中，然后将该部分暴露于标记复合物来定位标记物。

公开(公告)号：US4676980A

公开(公告)日：1987-06-30

申请号：US778670

申请日：1985-09-23

申请人： David M. Segal ,  Pilar Perez

发明人： David M. Segal ,  Pilar Perez

IPC分类号： C07K16/46 ,  G01N33/54 ,  G01N33/58 ,  G01N33/60

CPC分类号： C07K16/468 ,  C07K2317/732 ,  Y10S436/819

摘要： The present invention discloses a target specific cross-linked heteroantibody and a method of producing the same. The cross-linked heteroantibodies of the present invention can cause normal autologous cells of the immune system to destroy any unwanted cell for which an antibody is available. Treatment or control of tumors, viral infected cells, fungi, bacteria, parasites and the like is now made possible through the use of the heteroantibody complex of the present invention.

摘要翻译： 本发明公开了一种靶特异性交联异质体及其制造方法。 本发明的交联异抗体可引起免疫系统的正常自体细胞破坏抗体可用的任何不需要的细胞。 现在可以通过使用本发明的异抗体复合物来治疗或控制肿瘤，病毒感染的细胞，真菌，细菌，寄生虫等。

公开(公告)号：US4670382A

公开(公告)日：1987-06-02

申请号：US571129

申请日：1984-01-16

申请人： Helen R. Buckley ,  Michael T. Largen ,  Nancy A. Strockbine

发明人： Helen R. Buckley ,  Michael T. Largen ,  Nancy A. Strockbine

IPC分类号： A61K39/00 ,  A61K39/395 ,  A61P31/04 ,  C07K1/22 ,  C07K14/00 ,  C07K14/005 ,  C07K14/195 ,  C07K14/37 ,  C07K14/41 ,  C07K16/00 ,  C07K16/14 ,  C12N5/10 ,  C12N15/02 ,  C12P21/00 ,  C12P21/08 ,  C12R1/725 ,  C12R1/91 ,  G01N33/569 ,  G01N33/577 ,  G01N33/54 ,  C12N5/00 ,  C12N15/00

CPC分类号： C07K14/37 ,  C07K16/14 ,  Y10S435/948

摘要： Two novel hybridoma cell lines, ATCC #HB-8397 and ATCC #HB-8398 produce monoclonal antibody monospecific to a single determinant shared by a set of three closely related cytoplasmic antigens of Candida albicans. The antigens have molecular weights of 120-135 Kd, 48-52 Kd, and 35-38 Kd. The hybridomas are formed by fusing splenocytes from immunized BALB/c mice with SP2/O-Ag 14 myeloma cells. Monoclonal and monospecific, polyclonal antibodies to these cytoplasmic antigens find application in the immunodiagnosis of Candida infections.A procedure is provided for preparing partially purified cytoplasmic antigen of pathogenic Candida species for administration to splenocyte-donating mice. Also provided is a method for the biochemical purification of cytoplasmic antigen of a pathogenic Candida species used for the preparation of monoclonal and monospecific, polyclonal antisera thereto.

摘要翻译： 两种新型杂交瘤细胞系ATCC＃HB-8397和ATCC＃HB-8398产生单一抗体单特异性单个决定簇，由一组三个密切相关的白色念珠菌细胞质抗原共享。 抗原的分子量为120-135Kd，48-52Kd，和35-38Kd。 通过将免疫的BALB / c小鼠的脾细胞与SP2 / O-Ag14骨髓瘤细胞融合来形成杂交瘤。 针对这些细胞质抗原的单克隆和单特异性多克隆抗体可应用于念珠菌感染的免疫诊断。 提供了用于制备用于给予脾细胞供体小鼠的部分纯化的致病念珠菌种类的细胞质抗原的方法。 还提供了用于制备用于制备单克隆和单特异性多克隆抗血清的致病念珠菌物质的细胞质抗原的生物化学纯化的方法。

公开(公告)号：US4540660A

公开(公告)日：1985-09-10

申请号：US483055

申请日：1983-04-07

申请人： Richard A. Harte ,  Anthony B. Chen ,  Nancy K. Kaufman

发明人： Richard A. Harte ,  Anthony B. Chen ,  Nancy K. Kaufman

IPC分类号： C08L33/00 ,  A61K39/385 ,  A61K39/44 ,  C08L33/02 ,  C08L33/04 ,  C08L33/12 ,  G01N33/532 ,  G01N33/533 ,  G01N33/543 ,  G01N33/544 ,  G01N33/545 ,  G01N33/569 ,  G01N33/58 ,  G01N33/54

CPC分类号： G01N33/585 ,  G01N33/532 ,  G01N33/543 ,  G01N33/544 ,  G01N33/569 ,  Y10S435/968

摘要： A substrate for fluoroimmunoassay is disclosed comprising a swellable, rehydratable polymeric protein-binding material forming a three-dimensional matrix; first particulate, light scattering centers distributed in the polymeric matrix capable of scattering visible light; and second particulate, light-scattering centers distributed in the polymeric matrix capable of reflecting fluorescent excitation light and absorbing all other light. The substrate efficiently binds proteins and enhances transmitted fluorescent light and thus increases the sensitivity of assays involving fluorescent measurements. The polymeric protein binding material is preferable an acrylic copolymer. The first light scattering centers are preferably oxides of titanium and zinc, and the second light-scattering centers are preferably a phthalocyanin compound.

摘要翻译： 公开了用于荧光免疫测定的底物，其包含形成三维基质的可溶胀，可再水合的聚合蛋白结合材料; 分散在能够散射可见光的聚合物基质中的第一颗粒，光散射中心; 以及分布在能够反射荧光激发光并吸收所有其它光的聚合物基质中的第二颗粒状光散射中心。 底物有效地结合蛋白质并增强透射的荧光，从而增加涉及荧光测量的测定的灵敏度。 聚合蛋白结合材料优选为丙烯酸共聚物。 第一光散射中心优选为钛和锌的氧化物，第二光散射中心优选为酞菁化合物。

公开(公告)号：US4540659A

公开(公告)日：1985-09-10

申请号：US399107

申请日：1982-07-16

申请人： David J. Litman ,  Edwin F. Ullman

发明人： David J. Litman ,  Edwin F. Ullman

IPC分类号： G01N33/542 ,  G01N33/54 ,  C12N9/96

CPC分类号： G01N33/542 ,  Y10S435/966 ,  Y10S435/967 ,  Y10S436/824

摘要： An assay method and compositions are provided for determining the presence of an analyte in a sample. The analyte is a member of an immunological pair (mip) comprising ligand and receptor. By providing a first measurement surface capable of specifically binding a labelled reagent in an amount depending upon the presence of analyte in the sample and a second calibration surface capable of binding a second labeled reagent in a manner unaffected by the presence of analyte in the sample, calibration of individual tests can be accomplished simultaneously with the performance of the test itself. A signal producing system includes an enzyme, a catalyst usually bonded to a mip which defines the first labeled reagent for binding to the measurement surface and the same catalyst conjugated to a ligand capable of binding to the calibration surface. Preferably, both labeled reagents have the same composition and the calibration surface includes anti-(first catalyst).

摘要翻译： 提供测定方法和组合物以确定样品中分析物的存在。 分析物是包含配体和受体的免疫对（mip）的成员。 通过提供能够以取决于样品中分析物的存在的量特异性结合标记试剂的第一测量表面和能够以不受样品中分析物的存在影响的方式结合第二标记试剂的第二校准表面， 单独测试的校准可以与测试本身的性能同时完成。 信号产生系统包括酶，通常结合到mip上的催化剂，其定义用于结合测量表面的第一标记试剂和与能够结合校准表面的配体缀合的相同催化剂。 优选地，两个标记的试剂具有相同的组成，并且校准表面包括抗（第一催化剂）。

公开(公告)号：US4529700A

公开(公告)日：1985-07-16

申请号：US409856

申请日：1982-08-20

申请人： Howard G. Gratzner

发明人： Howard G. Gratzner

IPC分类号： C12N15/02 ,  C07K16/44 ,  C12N5/10 ,  C12P21/08 ,  G01N33/53 ,  C12N5/00 ,  C12N15/00 ,  C12Q1/68 ,  G01N33/54

CPC分类号： C07K16/44 ,  G01N33/5308 ,  Y10S435/948

摘要： Cells synthesizing DNA are detected in a rapid, non-radioactive assay using the monoclonal antibody reagent secreted by the hybridoma produced in accordance with the present invention. The assay is used to study DNA repair in cells that have been exposed to various environmental toxins, chemotherapeutic agents, and the like. The monoclonal antibody secreted by the hybridoma is a valuable reagent for research and diagnosis.

摘要翻译： 使用由根据本发明制备的杂交瘤分泌的单克隆抗体试剂，在快速，非放射性测定中检测合成DNA的细胞。 该测定用于研究已经暴露于各种环境毒素，化学治疗剂等的细胞中的DNA修复。 由杂交瘤分泌的单克隆抗体是研究和诊断的有价值的试剂。

公开(公告)号：US4529582A

公开(公告)日：1985-07-16

申请号：US454908

申请日：1982-12-30

申请人： Karen K. Brown ,  Sharon A. Bryant

发明人： Karen K. Brown ,  Sharon A. Bryant

IPC分类号： A61K49/00 ,  G01N33/569 ,  G01N33/48 ,  G01N33/54

CPC分类号： A61K49/00 ,  G01N33/56944

摘要： Group C streptococcal antibody levels in a serum sample can be determined via passive protection test in susceptible animals. Test is based on property of protective antibody in the serum sample to reduce the virulence of a group C streptococcal challenge and provides a means for detecting whether an animal (e.g. horse) is susceptible to infection by group C streptococcal organism.

摘要翻译： 血清样品中C组链球菌抗体水平可以通过易感动物的被动保护试验来测定。 测试基于血清样品中保护性抗体的性质，以降低C组链球菌攻击的毒力，并提供检测动物（例如马）是否易受C组链球菌生物感染的方法。

公开(公告)号：US4528267A

公开(公告)日：1985-07-09

申请号：US476440

申请日：1983-03-17

申请人： Emanuel Calenoff ,  Yuh-Geng Tsay ,  Ruth M. Jones ,  Scott, John R.

发明人： Emanuel Calenoff ,  Yuh-Geng Tsay ,  Ruth M. Jones ,  Scott, John R.

IPC分类号： G01N33/545 ,  G01N33/58 ,  G01N33/54

CPC分类号： G01N33/582 ,  G01N33/545 ,  Y10S436/809

摘要： An inhibition assay for measuring the potency of allergen extracts by incubating a mixture of allergen extract and reference allergen specific IgE in a buffered solution with an insoluble support to which reference allergen is adhered. The conjugated IgE adhering to the insoluble support is reacted with an enzyme labeled anti-IgE antibody and the enzyme label is contacted with a solution of a substrate which will yield a fluorescent product in the presence of the enzyme. The level of fluoresence in the solution is measured. The percentage of inhibition of the allergen specific IgE is determined from fluorescence levels measured for various extract concentrations.

摘要翻译： 通过将缓冲溶液中的变应原提取物和参比变应原特异性IgE的混合物与参与变应原粘附的不溶性载体进行温育来测量变应原提取物的效力的抑制试验。 附着于不溶性载体的缀合的IgE与酶标记的抗IgE抗体反应，并且酶标记物与底物的溶液接触，所述底物将在酶的存在下产生荧光产物。 测量溶液中的荧光水平。 由各种提取物浓度测量的荧光水平确定变应原特异性IgE的抑制百分比。

公开(公告)号：US4522918A

公开(公告)日：1985-06-11

申请号：US330959

申请日：1981-12-15

申请人： Jeffery Schlom ,  David Colcher ,  Marianna Nuti ,  Patricia H. Hand ,  Faye Austin

发明人： Jeffery Schlom ,  David Colcher ,  Marianna Nuti ,  Patricia H. Hand ,  Faye Austin

IPC分类号： A61K38/00 ,  A61K51/10 ,  C07K16/30 ,  C12P21/08 ,  C12P21/00 ,  A61K39/00 ,  A61K49/00 ,  C12N5/00 ,  C12N15/00 ,  C12R1/91 ,  G01N33/54

CPC分类号： A61K51/1051 ,  C07K16/3015 ,  A61K2123/00 ,  A61K38/00 ,  Y10S435/948 ,  Y10S435/96 ,  Y10S436/813

摘要： Monoclonal antibodies demonstrating a reactivity with human breast cancer are produced. The hybridoma cultures secreting immunoglobins are produced by hydridoma technology. Splenic lymphocytes of mice, immunized with membrane-enriched fractions of metastatic human mammary carcinoma tissue are fused with the NS-1 non-immunoglobulin-secreting murine myeloma cell line. Screening of immunoglobulin reactivities and double cloning of cultures yielded 11 monoclonal antibodies that demonstrated activities with the surface of human mammary tumor cells and not with the surface of apparently normal human tissues. These monoclonal antibodies aid in the diagnosis, prognosis and treatment of human breast cancer.

摘要翻译： 产生显示与人乳腺癌反应性的单克隆抗体。 分泌免疫球蛋白的杂交瘤培养物是通过胶体技术产生的。 用转移性人乳腺癌组织的膜富集部分免疫的小鼠的脾淋巴细胞与NS-1非免疫球蛋白分泌的小鼠骨髓瘤细胞系融合。 免疫球蛋白反应性的筛选和双克隆培养物产生了11种单克隆抗体，其表现出与人乳腺肿瘤细胞表面的活性，而不是表面上正常人组织的表面。 这些单克隆抗体有助于人乳腺癌的诊断，预后和治疗。

公开(公告)号：US4521510A

公开(公告)日：1985-06-04

申请号：US567890

申请日：1984-01-03

申请人： John E. Geltosky

发明人： John E. Geltosky

IPC分类号： C07K16/44 ,  C12P21/08 ,  G01N33/577 ,  C12P21/00 ,  A23J1/00 ,  C12N5/00 ,  G01N33/54

CPC分类号： C07K16/44 ,  G01N33/577 ,  Y10S435/948 ,  Y10S530/809

摘要： Monoclonal antibodies to theophylline having 5% or less cross-reactivity with caffeine and the continuous hybrid monoclonal cell lines for their production are provided. These antibodies are useful in a particle-enhanced turbidimetric inhibition immunoassay for theophylline.

摘要翻译： 提供了与咖啡因具有5％以下交叉反应性的茶碱单克隆抗体和用于其生产的连续杂交单克隆细胞系。 这些抗体可用于茶碱的颗粒增强比浊抑制免疫测定。