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公开(公告)号：US5747272A
公开(公告)日：1998-05-05
申请号：US412231
申请日：1995-03-10
申请人： Alison O'Brien , Susanne Ward Lindgren , Liyanage Parakrama Perera , Nancy A. Strockbine , Angela Ruth Melton-Celsa
发明人： Alison O'Brien , Susanne Ward Lindgren , Liyanage Parakrama Perera , Nancy A. Strockbine , Angela Ruth Melton-Celsa
IPC分类号： G01N33/569 , G01N33/58 , G01N33/53
CPC分类号： G01N33/56916 , G01N33/582 , G01N2333/25 , Y10S435/968 , Y10S435/975
摘要： A rapid, sensitive, non-radioactive diagnostic kit for the direct detection of both Shiga-like toxin, type I, and Shiga-like toxin, type II, produced by enterohemorrhagic Escherichia Coli in food and clinical samples. This diagnostic kit is comprised of a monoclonal antibody capable of detecting Shiga-like toxin, type I, and a monoclonal antibody capable of detecting Shiga-like toxin, type II, together with a chemiluminescing detection reagent with a sensitivity enhancer.
摘要翻译： 一种快速，灵敏，非放射性的诊断试剂盒，用于直接检测食源性和临床样品中肠出血性大肠杆菌产生的滋贺样毒素，I型和志贺样毒素II型。 该诊断试剂盒由能够检测类似I型的志贺样毒素的单克隆抗体和能够检测II型志贺样毒素的单克隆抗体与具有灵敏度增强剂的化学发光检测试剂组成。
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公开(公告)号：US4806465A
公开(公告)日：1989-02-21
申请号：US32111
申请日：1987-03-30
摘要： Two novel hybridoma cell lines, ATCC #HB-8397 and ATCC #HB-8398 produce monoclonal antibody monospecific to a single determinant shared by a set of three closely related cytoplasmic antigens of Candida albicans. The antigens have molecular weights of 120-135 Kd, 48-52 Kd, and 35-38 Kd. The hybridomas are formed by fusing splenocytes from immunized BALB/c mice with SP2/O-Ag 14 myeloma cells. Monoclonal and monospecific, polyclonal antibodies to these cytoplasmic antigens find application in the immunodiagnosis of Candida infections.A procedure is provided for preparing partially purified cytoplasmic antigen of pathogenic Candida species for administration to splenocyte-donating mice. Also provided is a method for the biochemical purification of cytoplasmic antigen of a pathogenic Candida species used for the preparation of monoclonal and monospecific, polyclonal antisera thereto.
摘要翻译： 两种新型杂交瘤细胞系ATCC＃HB-8397和ATCC＃HB-8398产生单一抗体单特异性单个决定簇，由一组三个密切相关的白色念珠菌细胞质抗原共享。 抗原的分子量为120-135Kd，48-52Kd，和35-38Kd。 通过将免疫的BALB / c小鼠的脾细胞与SP2 / O-Ag14骨髓瘤细胞融合来形成杂交瘤。 针对这些细胞质抗原的单克隆和单特异性多克隆抗体可应用于念珠菌感染的免疫诊断。 提供了用于制备用于给予脾细胞供体小鼠的部分纯化的致病念珠菌种类的细胞质抗原的方法。 还提供了用于制备用于制备单克隆和单特异性多克隆抗血清的致病念珠菌物质的细胞质抗原的生物化学纯化的方法。
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3.发明授权Monoclonal antibody to Candida albicans cytoplasmic antigens and methods of preparing same 失效白念珠菌细胞质抗原的单克隆抗体及其制备方法
公开(公告)号：US4670382A
公开(公告)日：1987-06-02
申请号：US571129
申请日：1984-01-16
IPC分类号： A61K39/00 , A61K39/395 , A61P31/04 , C07K1/22 , C07K14/00 , C07K14/005 , C07K14/195 , C07K14/37 , C07K14/41 , C07K16/00 , C07K16/14 , C12N5/10 , C12N15/02 , C12P21/00 , C12P21/08 , C12R1/725 , C12R1/91 , G01N33/569 , G01N33/577 , G01N33/54 , C12N5/00 , C12N15/00
CPC分类号： C07K14/37 , C07K16/14 , Y10S435/948
摘要： Two novel hybridoma cell lines, ATCC #HB-8397 and ATCC #HB-8398 produce monoclonal antibody monospecific to a single determinant shared by a set of three closely related cytoplasmic antigens of Candida albicans. The antigens have molecular weights of 120-135 Kd, 48-52 Kd, and 35-38 Kd. The hybridomas are formed by fusing splenocytes from immunized BALB/c mice with SP2/O-Ag 14 myeloma cells. Monoclonal and monospecific, polyclonal antibodies to these cytoplasmic antigens find application in the immunodiagnosis of Candida infections.A procedure is provided for preparing partially purified cytoplasmic antigen of pathogenic Candida species for administration to splenocyte-donating mice. Also provided is a method for the biochemical purification of cytoplasmic antigen of a pathogenic Candida species used for the preparation of monoclonal and monospecific, polyclonal antisera thereto.
摘要翻译： 两种新型杂交瘤细胞系ATCC＃HB-8397和ATCC＃HB-8398产生单一抗体单特异性单个决定簇，由一组三个密切相关的白色念珠菌细胞质抗原共享。 抗原的分子量为120-135Kd，48-52Kd，和35-38Kd。 通过将免疫的BALB / c小鼠的脾细胞与SP2 / O-Ag14骨髓瘤细胞融合来形成杂交瘤。 针对这些细胞质抗原的单克隆和单特异性多克隆抗体可应用于念珠菌感染的免疫诊断。 提供了用于制备用于给予脾细胞供体小鼠的部分纯化的致病念珠菌种类的细胞质抗原的方法。 还提供了用于制备用于制备单克隆和单特异性多克隆抗血清的致病念珠菌物质的细胞质抗原的生物化学纯化的方法。
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