公开(公告)号：US20230333108A1

公开(公告)日：2023-10-19

申请号：US18005178

申请日：2021-07-19

申请人： The Regents of the University of California

发明人： Drew A. Hall ,  Eliah Aronoff-Spencer ,  Naveen Singh ,  Partha Ray

IPC分类号： G01N33/569 ,  G01N33/543

CPC分类号： G01N33/56983 ,  G01N33/54326 ,  G01N33/54366 ,  G01N2333/165 ,  G01N2469/10

摘要： Disclosed are systems, devices and methods for a quantitative aptamer-based viral assay. In some aspects, an aptamer-based viral assay device includes a substrate and a biochemical complex conjugated to the substrate, which comprises an aptamer that is initially bound to an enzyme-tagged complementary strand of nucleotides, the aptamer corresponding to an antigen of a virus (e.g., SARS-CoV-2) that has a higher binding affinity to the aptamer than the complementary strand of nucleotides, wherein, when the device is exposed to a solution containing the virus, the enzyme-tagged strand is released from the aptamer as the aptamer binds the antigen of the virus, such that the released enzyme is capable of converting a substance to an analyte that is measurable by a remote analyte meter to correlate with a parameter of the virus in the solution.

公开(公告)号：US20230331825A1

公开(公告)日：2023-10-19

申请号：US18042639

申请日：2021-08-25

申请人： Target Discovery Merger Sub II, LLC

发明人： Goran Rakocevic ,  Daniele Biasci

IPC分类号： C07K16/10 ,  G01N33/531 ,  G01N33/569 ,  A61P31/14 ,  A61K45/06 ,  A61K39/42

CPC分类号： C07K16/1003 ,  G01N33/531 ,  G01N33/56983 ,  A61P31/14 ,  A61K45/06 ,  A61K39/42 ,  G01N2333/165 ,  G01N2469/10 ,  C07K2317/565 ,  C07K2317/34 ,  C07K2317/76

摘要： The disclosure herein relates to novel antibodies and antigen binding fragments that are used in the treatment, prevention and diagnosis of COVID-19, the disease caused by SARS-CoV-2. The complete polypeptide and nucleic acid consensus sequences of the antibodies and antigen binding fragments disclosed herein are reconstructed in silico.

公开(公告)号：US11787853B2

公开(公告)日：2023-10-17

申请号：US17499495

申请日：2021-10-12

申请人： MEDIMMUNE, LLC ,  HUMABS BIOMED SA

发明人： Nicole Kallewaard-Lelay ,  Qing Zhu ,  Ebony Benjamin ,  Leslie Wachter ,  Andy Yuan ,  Josephine Mary Mcauliffe ,  Davide Corti ,  Antonio Lanzavecchia

IPC分类号： C07K16/10 ,  A61P31/16 ,  G01N33/569 ,  A61K39/00

CPC分类号： C07K16/1018 ,  A61P31/16 ,  G01N33/56983 ,  A61K2039/505 ,  C07K2317/21 ,  C07K2317/33 ,  C07K2317/34 ,  C07K2317/515 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/732 ,  C07K2317/76 ,  C07K2317/92 ,  G01N2333/11 ,  G01N2469/10

摘要： The invention relates to antibodies and antigen binding fragments thereof that are capable of binding to influenza B virus hemagglutinin (HA) and neutralizing influenza B virus in two phylogenetically distinct lineages. In one embodiment, the antibody or antigen binding fragment is capable of binding to influenza B virus hemagglutinin and neutralizing influenza B virus in Yamagata and Victoria lineages.

公开(公告)号：US20230324387A1

公开(公告)日：2023-10-12

申请号：US18033925

申请日：2021-11-01

申请人： The Johns Hopkins University

发明人： Stephen J. Gould ,  Chenxu Guo ,  Shang-Jui Tsai

IPC分类号： G01N33/569 ,  G01N33/68

CPC分类号： G01N33/56983 ,  G01N33/6854 ,  G01N2333/165 ,  G01N2469/20

摘要： The present disclosure provides methods for the detection of target specific antibodies in samples. The methods include the detection of pathogen-specific antibodies, such as SARS-CoV-2 specific antibodies. Also included is a kit for use for the detection of pathogen specific antibodies in a sample. In one aspect, the disclosure provides a method of detecting the presence of a target specific antibody in a sample by (i) contacting the sample with a test cell comprising one or more exogenous nucleic acid sequences encoding one or more target proteins; and (ii) detecting the presence of the target specific antibody in the sample by contacting the immune complex of (i) with an anti-immunoglobulin (Ig) antibody, and detecting the anti-immunoglobulin (Ig) antibody, thereby detecting the presence of a target specific antibody.

公开(公告)号：US11780884B2

公开(公告)日：2023-10-10

申请号：US15734263

申请日：2020-02-14

申请人： Institut Virion \ Serion GmbH ,  Judith Stuermer

发明人： Thomas Schumacher ,  Hugo Arends

IPC分类号： C12N7/00 ,  C07K14/005 ,  A61K39/20 ,  G01N33/569

CPC分类号： C07K14/005 ,  A61K39/20 ,  G01N33/56983 ,  C12N2770/36222 ,  C12N2770/36234 ,  C12N2770/36251 ,  G01N2333/19 ,  G01N2469/20

摘要： The Rubella Virus Spike construct comprises at least one E1 component and one E2 component, which are linked together. The E1 component consists of the El envelope protein, whose C-terminal transmembrane region and intravirional domain are removed and whose N-terminus comprises the ectodomain of the El envelope protein. The E2 component consists of the E2 envelope protein whose transmembrane regions and intravirional domain removed and whose C-terminus comprising the ectodomain of the E2 envelope protein.
The C-terminus of the E2 component is connected to the N-terminus of the E1 component by direct fusion or by means of a linker to form an E1-E2 fusion protein.

公开(公告)号：US20230314433A1

公开(公告)日：2023-10-05

申请号：US18023065

申请日：2021-08-24

申请人： KURUME UNIVERSITY

发明人： Tomoaki HOSHINO

IPC分类号： G01N33/569

CPC分类号： G01N33/56983 ,  C12Y306/04013 ,  G01N2333/914 ,  G01N2470/04

摘要： The disclosure provides a method of determining whether a subject is infected with a virus, comprising (1) testing whether a protein comprising an amino acid sequence of at least a part of the helicase domain of MDA5 protein and having a molecular weight of about 60 kDa is detected in a sample obtained from the subject; and (2) determining the subject is infected with the virus when the protein was detected. The disclosure also provides a monoclonal antibody capable of binding to the protein.

公开(公告)号：US20230305006A1

公开(公告)日：2023-09-28

申请号：US18130523

申请日：2023-04-04

申请人： PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Feng XUAN ,  Yu Wang ,  Peng Yin ,  Tsz Wing Fan

IPC分类号： G01N33/569

CPC分类号： G01N33/56983 ,  G01N2333/165 ,  G01N2458/10 ,  G01N2469/20

摘要： Provided herein are methods, compositions, kits, and systems for the detection of neutralization targets such as neutralizing antibodies.

公开(公告)号：US20230305004A1

公开(公告)日：2023-09-28

申请号：US18020604

申请日：2021-08-16

申请人： The Regents of the University of California

发明人： Daniel Takashi Kamei ,  Daniel William Bradbury ,  Ren Sun ,  Yushen Du ,  Benjamin Ming Wu ,  Jasmine Thanh Trinh ,  Milo Ryan ,  Cassandra Marie Cantu

IPC分类号： G01N33/569 ,  G01N33/543 ,  C12M1/34

CPC分类号： G01N33/56983 ,  G01N33/54388 ,  C12M1/34 ,  G01N33/54346 ,  G01N2333/165

摘要： In various embodiments methods to improve the detection of a lateral-flow immunoassay for the sensitive detection of the SARS-CoV-2 nucleocapsid protein or other analytes, as well as devices that incorporate those methods are provided.

公开(公告)号：US20230303634A1

公开(公告)日：2023-09-28

申请号：US18076315

申请日：2022-12-06

申请人： Board of Regents, The University of Texas System

发明人： Hongjie Xia ,  Xuping Xie ,  Pei-Yong Shi

IPC分类号： C07K14/165 ,  G01N33/569

CPC分类号： C07K14/165 ,  G01N33/56983 ,  C07K2319/60

摘要： Certain embodiments of the invention include recombinant reverse genetic systems for Omicron variant of SARS-CoV-2 virus.

公开(公告)号：US20230296623A1

公开(公告)日：2023-09-21

申请号：US18017073

申请日：2021-07-16

申请人： Roche Diagnostics Operations, Inc.

发明人： Torbjoern Omland ,  Ursula-Henrike Wienhues-Thelen ,  Andre Ziegler

IPC分类号： G01N33/68 ,  G01N33/569

CPC分类号： G01N33/6893 ,  G01N33/56983 ,  G01N2800/56

摘要： The present invention relates to a method for predicting the disease severity in a patient with COVID-19, said method comprising a) determining the level of GDF-15 in a sample from the patient with COVID-19, b) comparing the level determined in step a) to a reference, and predicting the disease severity in a patient with COVID-19. Also encompassed are methods for risk stratification, monitoring of the disease progression and the risk prediction for hospital admission in a patient with COVID-19. Furthermore, methods are provided for predicting the need for intensive care, the risk for thrombosis, pulmonary embolism, hypoxia and mortality in a patient with COVID-19.