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公开(公告)号：US20160032260A1
公开(公告)日：2016-02-04
申请号：US14885727
申请日：2015-10-16
Applicant: ROCHE DIAGNOSTICS OPERATIONS, INC.
Inventor： Harald Sobek , Johann-Peter Thalhofer , Rainer Mueller , Manfred Schmidt , Michael Greif , Armin Ruf , Christian Rudolph
IPC: C12N9/12
CPC classification number: C12N9/1247 , C12P19/34 , C12Y207/07006
Abstract: The present invention provides improved variants of T7 RNA polymerase by introducing novel mutations which lead to improved thermostability of the enzyme. According to the invention, amino acid substitutions at the positions Val426, Ser633, Val650, Thr654, Ala702, Val795, and combinations thereof are advantageous.
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公开(公告)号：US20160017397A1
公开(公告)日：2016-01-21
申请号：US14694419
申请日：2015-04-23
Applicant: Sutro Biopharma, Inc.
Inventor： Sushmita Mimi Roy , Sunil Bajad , Evan Green , Henry Heinsohn
IPC: C12P21/06 , C12N9/12 , C07K14/535 , C12N1/20 , C12N1/06
CPC classification number: C12P21/06 , C07K14/535 , C12N1/06 , C12N1/20 , C12N9/1247 , C12P21/02 , C12Y207/07006 , G01N33/6848
Abstract: The present invention provides a method for monitoring of profile changes of components in a dynamic system such as a cell-free in vitro protein synthesis system by using liquid chromatography (LC) combined with mass spectrometry (MS). In an additional aspect, this invention provides a method for enhancing the yield and/or reproducibility in a cell-free protein synthesis system by modulating the level and/or activity of a protein component that has regulatory effects on the system.
Abstract translation: 本发明提供了一种通过使用液相色谱（LC）与质谱（MS）组合来监测动态系统（例如无细胞体外蛋白质合成系统）中组分的分布变化的方法。 在另一方面，本发明提供了通过调节对系统具有调节作用的蛋白质组分的水平和/或活性来提高无细胞蛋白质合成系统的产量和/或再现性的方法。
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公开(公告)号：US09045740B2
公开(公告)日：2015-06-02
申请号：US13775929
申请日：2013-02-25
Applicant: University of Massachusetts
Inventor： Craig T. Martin , Luis Ramirez-Tapia
CPC classification number: C12N9/127 , C12N9/1247 , C12P19/34 , C12P21/02 , C12Y207/07006
Abstract: The invention relates to modified T7-related RNA polymerases and methods of use thereof. In some embodiments, the invention relates to modified T7-related RNA polymerases that transcribe RNA with reduced abortive cycling and increased efficiency compared with native T7-related RNA polymerases.
Abstract translation: 本发明涉及修饰的T7相关RNA聚合酶及其使用方法。 在一些实施方案中，本发明涉及经修饰的T7相关RNA聚合酶，其与天然T7相关RNA聚合酶相比转录RNA，具有降低的流产循环和增加的效率。
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84.发明授权Engineered enzymatically active bacteriophage and methods for dispersing biofilms 有权工程化的酶活性噬菌体和分散生物膜的方法公开(公告)号：US08153119B2
公开(公告)日：2012-04-10
申请号：US12337677
申请日：2008-12-18
Applicant: James J Collins , Timothy Kuan-Ta Lu
Inventor： James J Collins , Timothy Kuan-Ta Lu
CPC classification number: A01N63/02 , C12N7/00 , C12N9/2402 , C12N2795/10211 , C12N2795/10221 , C12N2795/10232 , C12Y207/07006 , C12Y302/01052
Abstract: The present invention is directed to engineered enzymatically active bacteriophages that are both capable of killing the bacteria by lysis and dispersing the bacterial biofilm because they have been also engineered to express biofilm-degrading enzymes, particularly dispersin B (DspB), an enzyme that hydrolyzes β-1,6-N-acetyl-D-glucosamine, a crucial adhesion molecule needed for biofilm formation and integrity in Staphylococcus and E. coli, including E. coli K-12, as well as clinical isolates.
Abstract translation: 本发明涉及工程化的酶活性噬菌体，其能够通过裂解和分散细菌生物膜来杀死细菌，因为它们也被工程化以表达生物降膜酶，特别是分解B（DspB），其是水解＆bgr的酶 ; -1,6-N-乙酰基-D-葡糖胺，生物膜形成所需的关键粘附分子和葡萄球菌和大肠杆菌（包括大肠杆菌K-12）以及临床分离物中的完整性。
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公开(公告)号：US20110262946A1
公开(公告)日：2011-10-27
申请号：US13087075
申请日：2011-04-14
Applicant: Sushmita Mimi Roy , Sunil Bajad , Evan Green , Henry Heinsohn
Inventor： Sushmita Mimi Roy , Sunil Bajad , Evan Green , Henry Heinsohn
CPC classification number: C12P21/06 , C07K14/535 , C12N1/06 , C12N1/20 , C12N9/1247 , C12P21/02 , C12Y207/07006 , G01N33/6848
Abstract: The present invention provides a method for monitoring of profile changes of components in a dynamic system such as a cell-free in vitro protein synthesis system by using liquid chromatography (LC) combined with mass spectrometry (MS). In an additional aspect, this invention provides a method for enhancing the yield and/or reproducibility in a cell-free protein synthesis system by modulating the level and/or activity of a protein component that has regulatory effects on the system.
Abstract translation: 本发明提供了一种通过使用液相色谱（LC）与质谱（MS）组合来监测动态系统（例如无细胞体外蛋白质合成系统）中组分的分布变化的方法。 在另一方面，本发明提供了通过调节对系统具有调节作用的蛋白质组分的水平和/或活性来提高无细胞蛋白质合成系统的产量和/或再现性的方法。
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Patent Agency Ranking
公开(公告)号：US20110256589A1
公开(公告)日：2011-10-20
申请号：US13069514
申请日：2011-03-23
Applicant: Harald Sobek , Johann-Peter Thalhofer , Rainer Mueller , Manfred Schmidt , Michael Greif , Armin Ruf , Christian Rudolph
Inventor： Harald Sobek , Johann-Peter Thalhofer , Rainer Mueller , Manfred Schmidt , Michael Greif , Armin Ruf , Christian Rudolph
CPC classification number: C12N9/1247 , C12P19/34 , C12Y207/07006
Abstract: The present invention provides improved variants of T7 RNA polymerase by introducing novel mutations which lead to improved thermostability of the enzyme. According to the invention, amino acid substitutions at the positions Val426, Ser633, Val650, Thr654, Ala702, Val795, and combinations thereof are advantageous.
Abstract translation: 本发明通过引入导致酶的热稳定性的新突变来提供T7RNA聚合酶的改进变体。 根据本发明，位置Val426，Ser633，Val650，Thr654，Ala702，Val795及其组合的氨基酸取代是有利的。
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87.发明申请COMPOSITIONS AND METHODS FOR ENHANCED SENSITIVITY AND SPECIFICITY OF NUCLEIC ACID SYNTHESIS 有权增强敏感性和核酸合成特异性的组合物和方法公开(公告)号：US20090275100A1
公开(公告)日：2009-11-05
申请号：US12468036
申请日：2009-05-18
Applicant: Mekbib Astatke , Deb K. Chatterjee , Gary F. Gerard
Inventor： Mekbib Astatke , Deb K. Chatterjee , Gary F. Gerard
IPC: C12N9/99
CPC classification number: C12N15/11 , A61K31/7052 , C12N9/1247 , C12N9/1252 , C12N9/1276 , C12N2310/122 , C12N2310/315 , C12P19/34 , C12Q1/6844 , C12Q1/6869 , C12Y207/07006 , C12Y207/07007 , C12Y207/07049 , C12Q2527/125
Abstract: The present invention relates to nucleic acid inhibitors, compositions and method for enhancing synthesis of nucleic acid molecules. In a preferred aspect, the invention relates to inhibition or control of nucleic acid synthesis, sequencing or amplification. Specifically, the present invention discloses nucleic acids having affinity for polypeptides with polymerase activity for use in such synthesis, amplification or sequencing reactions. The nucleic acid inhibitors are capable of inhibiting nonspecific nucleic acid synthesis under certain conditions (e.g., at ambient temperatures). Thus, in a preferred aspect, the invention relates to “hot start” synthesis of nucleic acid molecules. Accordingly, the invention prevents, reduces or substantially reduces nonspecific nucleic acid synthesis. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the nucleic acid inhibitors or compositions of the invention. The invention also relates to using the inhibitors of the invention to prevent viral replication or treat viral infections in a subject. Thus, the invention relates to therapeutic methods and pharmaceutical compositions using the inhibitors of the invention. The invention thus may be used for in vivo and in vitro inhibition of nucleic acid synthesis and/or inhibition of polymerase activity.
Abstract translation: 本发明涉及用于增强核酸分子合成的核酸抑制剂，组合物和方法。 在优选的方面，本发明涉及抑制或控制核酸合成，测序或扩增。 具体而言，本发明公开了对于在这种合成，扩增或测序反应中使用具有聚合酶活性的多肽具有亲和力的核酸。 核酸抑制剂能够在某些条件下（例如在环境温度下）抑制非特异性核酸合成。 因此，在优选的方面，本发明涉及核酸分子的“热启动”合成。 因此，本发明防止，减少或显着降低非特异性核酸合成。 本发明还涉及用于合成，扩增，逆转录或测序核酸分子的试剂盒，所述核酸分子包含一种或多种本发明的核酸抑制剂或组合物。 本发明还涉及使用本发明的抑制剂来预防病毒复制或治疗受试者的病毒感染。 因此，本发明涉及使用本发明的抑制剂的治疗方法和药物组合物。 因此，本发明可用于体内和体外抑制核酸合成和/或抑制聚合酶活性。
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88.发明申请Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis 审中-公开增强核酸合成灵敏度和特异性的组合物和方法公开(公告)号：US20050089922A1
公开(公告)日：2005-04-28
申请号：US11007184
申请日：2004-12-09
Applicant: Mekbib Astatke , Deb Chatterjee , Gary Gerard
Inventor： Mekbib Astatke , Deb Chatterjee , Gary Gerard
CPC classification number: C12N15/11 , A61K31/7052 , C12N9/1247 , C12N9/1252 , C12N9/1276 , C12N2310/122 , C12N2310/315 , C12P19/34 , C12Q1/6844 , C12Q1/6869 , C12Y207/07006 , C12Y207/07007 , C12Y207/07049 , C12Q2527/125
Abstract: The present invention relates to nucleic acid inhibitors, compositions and method for enhancing synthesis of nucleic acid molecules. In a preferred aspect, the invention relates to inhibition or control of nucleic acid synthesis, sequencing or amplification. Specifically, the present invention discloses nucleic acids having affinity for polypeptides with polymerase activity for use in such synthesis, amplification or sequencing reactions. The nucleic acid inhibitors are capable of inhibiting nonspecific nucleic acid synthesis under certain conditions (e.g., at ambient temperatures). Thus, in a preferred aspect, the invention relates to “hot start” synthesis of nucleic acid molecules. Accordingly, the invention prevents, reduces or substantially reduces nonspecific nucleic acid synthesis. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the nucleic acid inhibitors or compositions of the invention. The invention also relates to using the inhibitors of the invention to prevent viral replication or treat viral infections in a subject. Thus, the invention relates to therapeutic methods and pharmaceutical compositions using the inhibitors of the invention. The invention thus may be used for in vivo and in vitro inhibition of nucleic acid synthesis and/or inhibition of polymerase activity.
Abstract translation: 本发明涉及用于增强核酸分子合成的核酸抑制剂，组合物和方法。 在优选的方面，本发明涉及抑制或控制核酸合成，测序或扩增。 具体而言，本发明公开了对于在这种合成，扩增或测序反应中使用具有聚合酶活性的多肽具有亲和力的核酸。 核酸抑制剂能够在某些条件下（例如在环境温度下）抑制非特异性核酸合成。 因此，在优选的方面，本发明涉及核酸分子的“热启动”合成。 因此，本发明防止，减少或显着降低非特异性核酸合成。 本发明还涉及用于合成，扩增，逆转录或测序核酸分子的试剂盒，所述核酸分子包含一种或多种本发明的核酸抑制剂或组合物。 本发明还涉及使用本发明的抑制剂来预防病毒复制或治疗受试者的病毒感染。 因此，本发明涉及使用本发明的抑制剂的治疗方法和药物组合物。 因此，本发明可用于体内和体外抑制核酸合成和/或抑制聚合酶活性。
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公开(公告)号：US06355469B1
公开(公告)日：2002-03-12
申请号：US09082920
申请日：1998-01-16
Applicant: Kelvin T. Lam
Inventor： Kelvin T. Lam
IPC: C12N120
CPC classification number: C12N9/1247 , C12Y207/07006
Abstract: The invention relates to Mycobacterium tuberculosis RNA polymerase algU sigma subunit protein, DNA encoding, and methods of detecting inhibitors of the RNA polymerase.
Abstract translation: 本发明涉及结核分枝杆菌RNA聚合酶algU sigma亚基蛋白，DNA编码和检测RNA聚合酶抑制剂的方法。
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公开(公告)号：US20240141403A1
公开(公告)日：2024-05-02
申请号：US18523908
申请日：2023-11-30
Applicant: The Regents of the University of California
Inventor： Alanna Schepartz Shrader , Sebasthian Santiago González
CPC classification number: C12P21/02 , C12N9/1247 , C12N9/93 , C12N15/74 , C12Y207/07006 , C12Y601/01007 , C12N2800/101
Abstract: Recombinant proteins comprising a non-canonical amino acid with high yield and high fidelity are made by expressing the protein in an engineered Vibrio natriegens strain containing an orthogonal translation system comprising an orthogonal aminoacyl tRNA synthetase that charges the non-canonical amino acid onto the orthogonal cognate tRNA.
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