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    Enzymatic oil recuperation process
    81.
    发明申请
    Enzymatic oil recuperation process 有权
    酶油回收过程

    公开(公告)号：US20100240917A1

    公开(公告)日：2010-09-23

    申请号：US12661429

    申请日：2010-03-17

    Applicant: Marc Kellens ,  Wim De Greyt

    Inventor： Marc Kellens ,  Wim De Greyt

    IPC: C07C59/00 ,  C12P7/64

    CPC classification number: C12P7/6445 ,  C12P7/6418

    Abstract: A process for the recuperation of acylglycerols or acylglycerols containing free fatty acids from gums, present as aqueous emulsions, said gums being obtained by subjecting triglyceride oil to one or more degumming processes, said recuperation process comprising the steps of: subjecting said gums to enzymatic hydrolysis catalysed by one or more enzymes with phospholipase activity; allowing said gums to separate into two or more phases, said two or more phases including at least an oily phase and an aqueous phase; and recuperating said oily phase comprising acylglycerols or acylglycerols containing free fatty acids; wherein said enzymatic hydrolysis is accelerated by adding at least part of the one or more enzymes with phospholipase activity to at least part of the water used in the recuperation and/or at least one degumming process; and/or at least part of said triglyceride oils treated in said degumming process; and/or by facilitating mixing at least part of said one or more enzymes with phospholipase activity into said gums by increasing the triglyceride oil content of said gums; and an oil obtained by this process.

    Abstract translation: 一种由作为水性乳液存在的来自树胶的含有游离脂肪酸的酰基甘油或酰基甘油的回收方法，所述树胶通过使甘油三酯油进行一种或多种脱胶方法而获得，所述还原方法包括以下步骤：使所述树胶经酶促水解 由一种或多种具有磷脂酶活性的酶催化; 允许所述树胶分离成两个或更多个相，所述两个或更多个相包括至少一个油相和一个水相; 并使包含含有游离脂肪酸的酰基甘油或酰基甘油的所述油相回收; 其中所述酶水解通过将至少部分一种或多种具有磷脂酶活性的酶加到至少部分用于还原和/或至少一种脱胶过程中的水中来加速; 和/或在所述脱胶过程中处理的所述甘油三酯油的至少一部分; 和/或通过增加所述牙龈的甘油三酯油含量，促进将至少部分所述一种或多种酶与磷脂酶活性混合到所述牙龈中; 和通过该方法获得的油。

    Method for removing enzyme and method of base exchange or hydrolysis of phospholipid using the same
    84.
    发明授权
    Method for removing enzyme and method of base exchange or hydrolysis of phospholipid using the same 有权
    消除酶的方法和使用其的磷脂的碱交换或水解的方法

    公开(公告)号：US07709238B2

    公开(公告)日：2010-05-04

    申请号：US10592858

    申请日：2005-03-18

    Applicant: Xiaoli Liu ,  Naruyuki Taniwaki

    Inventor： Xiaoli Liu ,  Naruyuki Taniwaki

    IPC: C12N9/99 ,  C12N9/00 ,  C12N9/20 ,  C12N9/48 ,  C12N9/50

    CPC classification number: C07F9/103 ,  C07F9/091 ,  C07F9/106 ,  C12N9/16 ,  C12P7/6418 ,  C12P7/6481

    Abstract: A method of removing an enzyme from a liquid enzyme reaction mixture used in a hydrolysis reaction or a base exchange reaction of a phospholipid is provided. The method includes the step of treating the liquid enzyme reaction mixture with a solvent mixture of water and an organic solvent, wherein the solvent mixture includes an inorganic metal salt, to remove the enzyme. Enzymes included in the reaction product can be easily removed without a treatment such as heating, and thus it becomes possible to easily produce various phospholipids that have a reduced risk of inducing an allergy, that retain a high quality and that have excellent storage stability.

    Abstract translation: 提供了从用于磷脂的水解反应或碱交换反应的液体酶反应混合物中除去酶的方法。 该方法包括用水和有机溶剂的溶剂混合物处理液体酶反应混合物的步骤，其中溶剂混合物包括无机金属盐，以除去酶。 包含在反应产物中的酶可以容易地除去而不进行诸如加热的处理，因此可以容易地生产出具有降低的诱导过敏风险的各种磷脂，其保持高质量并且具有优异的储存稳定性。

    METHOD FOR PRODUCING A USEFUL SUBSTANCE BY USE OF AN IMMOBILIZED ENZYME
    86.
    发明申请
    METHOD FOR PRODUCING A USEFUL SUBSTANCE BY USE OF AN IMMOBILIZED ENZYME 失效
    通过使用固定化酶生产有用物质的方法

    公开(公告)号：US20090298142A1

    公开(公告)日：2009-12-03

    申请号：US12067664

    申请日：2006-10-04

    Applicant: Jun Saito ,  Yoshitaka Senda ,  Toshiteru Komatsu

    Inventor： Jun Saito ,  Yoshitaka Senda ,  Toshiteru Komatsu

    IPC: C12P7/64 ,  C12P1/00

    CPC classification number: C12N9/20 ,  C12N11/00 ,  C12P7/6418

    Abstract: The present invention provides a method for producing a useful substance by supplying, to a fixed-bed reactor packed with an immobilized enzyme, a liquid mixture containing two liquid phases, in which the two liquid phases are allowed to flow in an identical, parallel direction, which method employs a fixed-bed reactor equipped with an insertion unit or tubes, so as to form a plurality of lumens in the fixed-bed reactor, each lumen having a cross section of a circular shape with a diameter of 100 mm or less or having a polygonal shape with a diagonal line of 100 mm or less, wherein the lumens are packed with an immobilized enzyme and the liquid mixture is supplied therethrough. In a reaction performed by passing a reaction mixture exhibiting two liquid phases through a fixed-bed reactor equipped with an immobilized enzyme, overall flow of the reaction liquid is made uniform, to thereby facilitate production of a useful substance in an efficient manner.

    Abstract translation: 本发明提供一种制造有用物质的方法，该方法通过向固定化酶反应器中填充固定化酶，提供含有两个液相的液体混合物，其中允许两个液相沿相同的平行方向流动 该方法采用装备有插入单元或固定床的固定床反应器，以在固定床反应器中形成多个内腔，每个管腔具有直径为100mm以下的圆形截面 或具有对角线为100mm或更小的多边形形状，其中，腔内填充有固定化酶，并且通过其提供液体混合物。 在通过使具有两个液相的反应混合物通过装有固定化酶的固定床反应器进行的反应中，使反应液体的整体流动均匀，从而有效地制备有用物质。

    ENZYMATIC OIL-DEGUMMING METHOD
    88.
    发明申请
    ENZYMATIC OIL-DEGUMMING METHOD 有权
    ENIYYY油脂脱水方法

    公开(公告)号：US20070298157A1

    公开(公告)日：2007-12-27

    申请号：US11623689

    申请日：2007-01-16

    Applicant: Jorn Soe ,  Mark Turner

    Inventor： Jorn Soe ,  Mark Turner

    IPC: A23D9/00 ,  C12N9/10

    CPC classification number: C12N9/1029 ,  A21D8/042 ,  A23J7/00 ,  C11B3/003 ,  C12N9/20 ,  C12P7/6418 ,  C12P7/6445 ,  C12P7/6481 ,  C12Y301/01003

    Abstract: A process of enzymatic degumming edible oils, comprising treating edible oil with a lipid acyltransferase so as to transfer an acyl group from a major part of the phospholipid to one or more acyl acceptors, wherein the acyl acceptor may be any compound comprising a hydroxyl group. In one embodiment preferably the acyl acceptor is water and in another embodiment preferably the acyl acceptor is one or more sterols and/or stanols. When the acyl acceptor is a stanol and/or sterol, one or more sterol esters and/or stanol esters are produced. The lipid acyltransferase for use in the process of the present invention may comprise one or more of the following amino acid sequences: SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, or SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 36, SEQ ID NO: 38, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 45, SEQ ID NO: 47, SEQ ID NO: 50 or an amino acid sequence which has 75% or more identity thereto. A novel lipid acyltransferase comprising the amino acid sequence shown as SEQ ID NO: 16 is also taught.

    Abstract translation: 一种酶促脱脂食用油的方法，包括用脂质酰基转移酶处理食用油，以将酰基从大部分磷脂转移到一个或多个酰基受体上，其中酰基受体可以是包含羟基的任何化合物。 在一个实施方案中，优选地，酰基受体是水，在另一个实施方案中，优选酰基受体是一种或多种甾醇和/或甾烷醇。 当酰基受体是甾烷醇和/或甾醇时，产生一种或多种甾醇酯和/或甾烷醇酯。 用于本发明方法的脂质酰基转移酶可以包含一个或多个以下氨基酸序列：SEQ ID NO：1，SEQ ID NO：3，SEQ ID NO：4，SEQ ID NO：5，SEQ ID NO： SEQ ID NO：7，SEQ ID NO：8，SEQ ID NO：9，SEQ ID NO：10，SEQ ID NO：11，SEQ ID NO：12，SEQ ID NO：13， 14，或SEQ ID NO：15，SEQ ID NO：16，SEQ ID NO：17，SEQ ID NO：18，SEQ ID NO：36，SEQ ID NO：38，SEQ ID NO：40，SEQ ID NO：41 ，SEQ ID NO：45，SEQ ID NO：47，SEQ ID NO：50或与其同一性为75％以上的氨基酸序列。 还教导了包含SEQ ID NO：16所示氨基酸序列的新型脂质酰基转移酶。

    Esterification or hydrolysis with substrate treated un-dried immobilized lipolytic enzyme
    90.
    发明授权
    Esterification or hydrolysis with substrate treated un-dried immobilized lipolytic enzyme 失效
    用底物处理的未干燥的固定化脂肪分解酶进行酯化或水解

    公开(公告)号：US06716610B2

    公开(公告)日：2004-04-06

    申请号：US09453078

    申请日：1999-12-02

    Applicant: Masami Shimizu ,  Toshiteru Komatsu ,  Masao Shimizu ,  Minoru Kase

    Inventor： Masami Shimizu ,  Toshiteru Komatsu ,  Masao Shimizu ,  Minoru Kase

    IPC: C12P764

    CPC classification number: C12P7/6418 ,  C12N11/08 ,  C12P7/6454

    Abstract: A lipolytic enzyme such as lipase is adsorbed on a porous anion-exchange resin carrier to obtain an immobilized enzyme without drying. The un-dried immobilized enzyme is contacted with a fat or oil for a time which may be about 2 hours to about 24 hours to stabilize the immobilized enzyme, and the immobilized enzyme is recovered from the fat or oil. The recovered immobilized enzyme is then used for esterifying or hydrolyzing a substrate. Water content of the un-dried immobilized enzyme may be 20% or more be weight such as 20-60%. Prior to immobilization, the resin carrier may be treated with a lypophylic aliphatic acid which preferably has 8 to 18 carbons.

    Abstract translation: 将脂肪酶等脂肪分解酶吸附在多孔阴离子交换树脂载体上，得到不干燥的固定化酶。 将未干燥的固定化酶与脂肪或油接触约2小时至约24小时以稳定固定化酶的时间，并且从脂肪或油中回收固定化酶。 然后将回收的固定化酶用于酯化或水解底物。 未干燥固定化酶的含水量可以为20〜20重量％的20重量％以上。 在固定之前，树脂载体可以用优选具有8至18个碳原子的脂环族脂族酸处理。

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