公开(公告)号：US20110045016A1

公开(公告)日：2011-02-24

申请号：US12808028

申请日：2008-12-22

申请人： Ricardo Tostes Gazzinelli ,  Rafael Polidoro Alves Barbosa ,  Braulia Costa Caetano ,  Alexandre de Magalhaes Vieira Machado ,  Oscar Bruna-Romero ,  Flavio Fonseca Guimaraes ,  Erica Araujo Mendes

发明人： Ricardo Tostes Gazzinelli ,  Rafael Polidoro Alves Barbosa ,  Braulia Costa Caetano ,  Alexandre de Magalhaes Vieira Machado ,  Oscar Bruna-Romero ,  Flavio Fonseca Guimaraes ,  Erica Araujo Mendes

IPC分类号： A61K39/002 ,  C12N15/74 ,  C12N7/01 ,  A61P33/02 ,  A61P37/04 ,  C12N15/87

CPC分类号： A61K39/012 ,  A61K2039/5256 ,  A61K2039/545 ,  C12N2710/24143 ,  C12N2760/16011

摘要： The present invention concerns to recombinant influenza viruses and modified Vaccinia Ankara viruses (MVA), and to a process for construction of recombinant influenza viruses and modified vaccinia Ankara viruses (MVA) with genes that encode for the T. gondii parasite SAGI (MVA) and SAG2 (MVA and influenza) proteins, by means of a homologous recombination technique between two transfer vectors (for construction of MVA virus) and reverse genetics (for construction of influenza virus). Additionally, the present invention describes a vaccine composition using recombinant influenza viruses and modified vaccinia Ankara viruses (MVA), or recombinant adenoviruses and modified vaccinia Ankara viruses (MVA), for immunization against infections caused by the T. gondii parasite.

摘要翻译： 本发明涉及重组流感病毒和改良的牛痘安卡拉病毒（MVA），以及用于构建重组流感病毒和修饰的痘苗安卡拉病毒（MVA）的方法，其具有编码弓形虫寄生虫SAGI（MVA）和 SAG2（MVA和流感）蛋白，通过两个转移载体之间的同源重组技术（用于构建MVA病毒）和反向遗传学（用于构建流感病毒）。 另外，本发明描述了使用重组流感病毒和修饰的痘苗安卡拉病毒（MVA）或重组腺病毒和修饰的痘苗安卡拉病毒（MVA）的疫苗组合物，用于免疫针对由弓形虫寄生虫引起的感染。

公开(公告)号：US20100183668A1

公开(公告)日：2010-07-22

申请号：US12593851

申请日：2008-03-28

申请人： Xun Suo ,  Xiaojia Wang ,  Xianyong Liu ,  Tuanyuan Shi ,  Lili Hao ,  Wenchao Yan ,  Hongyan Wang ,  Jianan Li

发明人： Xun Suo ,  Xiaojia Wang ,  Xianyong Liu ,  Tuanyuan Shi ,  Lili Hao ,  Wenchao Yan ,  Hongyan Wang ,  Jianan Li

IPC分类号： A61K39/145 ,  A61K39/012 ,  A61P37/04

CPC分类号： A61K39/012 ,  A61K35/68 ,  A61K39/00 ,  A61K39/12 ,  A61K39/145 ,  A61K2039/522 ,  A61K2039/523 ,  A61K2039/542 ,  A61K2039/552 ,  C12N2760/16134 ,  Y02A50/489

摘要： The present invention provides a novel use of coccidian, specifically relates to the use of coccidian as a vaccine live vector. The present invention further provides a live vaccine with coccidian as a vector, which is transgenic coccidian capable of expressing exogenous protein or stably transfected coccidian that contain expression vector and can express exogenous coccidian. The present coccidian vector live vaccine can induce organisms to simultaneously generate protective humoral and cellular immune responses (including the mucosal immune response), as well as generate memory responses, which can be readily carried out and has stable effect and high biological safety without generating immune tolerance.

摘要翻译： 本发明提供了球虫的新用途，具体涉及球虫作为疫苗活载体的用途。 本发明还提供具有球虫作为载体的活疫苗，其是能够表达含有表达载体并能表达外源球虫的外源蛋白或稳定转染的球虫的转基因球虫。 本发明的球虫载体活疫苗可以诱导生物体同时产生保护性体液和细胞免疫应答（包括粘膜免疫应答），并产生记忆反应，其可以容易地进行并且具有稳定的效果和高生物安全性而不产生免疫 公差。

公开(公告)号：US07393534B2

公开(公告)日：2008-07-01

申请号：US10892659

申请日：2004-07-15

申请人： Charles Aylsworth ,  Siu-Cheong Ho ,  David Juckett ,  John W. Judge ,  Igor V. Zlatkin ,  Tatiana Zlatkin

发明人： Charles Aylsworth ,  Siu-Cheong Ho ,  David Juckett ,  John W. Judge ,  Igor V. Zlatkin ,  Tatiana Zlatkin

IPC分类号： A61K38/00 ,  A61K39/00

CPC分类号： A61K39/39 ,  A61K38/16 ,  A61K39/012 ,  A61K2039/55516 ,  C07K14/455

摘要： The present invention provides compositions and methods for the prevention and treatment of primary and metastatic neoplastic diseases and infectious diseases, for stimulating an immune response in a subject, and for use as an alternative to interleukin-12 (IL-12) treatment. In particular, the present invention provides Apicomplexa-related proteins (ARPs) that have immune stimulatory activity and thus have uses in the treatment and prevention of cancer and infectious diseases and in immune modulation. Compositions comprising an ARP are provided. Methods of use of an ARP for the prevention and/or treatment of cancer and/or infectious diseases, for use as an alternative to interleukin-12 (IL-12) treatment, and for eliciting an immune response in a subject, are also provided.

摘要翻译： 本发明提供用于预防和治疗原发性和转移性肿瘤疾病和感染性疾病，用于刺激受试者的免疫应答并用作白细胞介素-12（IL-12）治疗的替代物的组合物和方法。 特别地，本发明提供了具有免疫刺激活性的Apicocplexa相关蛋白（ARP），因此具有用于治疗和预防癌症和感染性疾病以及免疫调节的用途。 提供了包含ARP的组合。 还提供了用于预防和/或治疗癌症和/或感染性疾病（用作白细胞介素-12（IL-12）治疗的替代物）和用于在受试者中引发免疫应答的方法 。

公开(公告)号：US20080124363A1

公开(公告)日：2008-05-29

申请号：US11962584

申请日：2007-12-21

申请人： David Bzik ,  Barbara Fox

发明人： David Bzik ,  Barbara Fox

IPC分类号： A61K39/00 ,  C07H21/00 ,  C12Q1/02 ,  A61P43/00 ,  C12N1/10

CPC分类号： A61K39/012 ,  A61K2039/522 ,  C12N9/93 ,  G01N33/56905 ,  Y02A50/58

摘要： Uracil auxotroph mutants of apicomplexans are provided which lack a functional carbamoyl phosphate synthase II (CPSII) enzyme. Also provided are T. gondii autoxtroph mutants which express exogenous antigens, and methods of protecting an animal against a T. gondii and non-T. gondii disease.

摘要翻译： 提供了缺乏功能性氨基甲酰磷酸合成酶II（CPSII）酶的复合物的尿嘧啶营养缺陷型突变体。 还提供了表达外源抗原的弓形虫自体突变体，以及保护动物抵抗弓形虫和非T型的方法。 弓形虫病

公开(公告)号：US20080031896A1

公开(公告)日：2008-02-07

申请号：US11732674

申请日：2007-04-04

申请人： Arnoldus Vermeulen ,  Theodorus Schetters

发明人： Arnoldus Vermeulen ,  Theodorus Schetters

IPC分类号： A61K39/012 ,  A61K39/02 ,  A61K39/12 ,  A61P33/02 ,  C12N1/10

CPC分类号： C12R1/90 ,  A61K39/012 ,  A61K2035/11 ,  A61K2039/52

摘要： Described are coccidia strains and microbiological cultures comprising such strains. The coccidia strains are selected for tolerance to one group of coccidiostatic drugs, sensitivity to a second group of coccidiostatic drugs, and decreased proliferative capacity. Also described are compositions and vaccines based on such coccidia strain.

摘要翻译： 描述了包含这种菌株的球虫菌株和微生物培养物。 选择球虫菌株对一组抗球虫药物的耐受性，对第二组球虫抑制药物的敏感性和增殖能力降低。 还描述了基于这种球虫菌株的组合物和疫苗。

公开(公告)号：US07230075B1

公开(公告)日：2007-06-12

申请号：US09390846

申请日：1999-09-14

申请人： Jacobus Johannes Kok ,  Paul van den Boogaart ,  Arnodus Nicolaas Vermeulen

发明人： Jacobus Johannes Kok ,  Paul van den Boogaart ,  Arnodus Nicolaas Vermeulen

IPC分类号： C07K1/00 ,  C07K14/00 ,  A01N37/18 ,  A61K39/00 ,  A61K39/38 ,  A61K38/00 ,  A61K39/012

CPC分类号： A61K39/012 ,  A61K38/00 ,  A61K39/00 ,  A61K2039/51 ,  A61K2039/53 ,  C07K14/455 ,  C07K16/20 ,  C12N9/0006

摘要： This invention relates to novel Eimeria proteins with immunogenic properties as well as to DNA sequences encoding these proteins. These proteins can be administered to poultry thereby protecting the birds against coccidiosis. In addition the DNA encoding these proteins can be used for the preparation of a vector vaccine against coccidiosis.

摘要翻译： 本发明涉及具有免疫原性的新颖艾美球虫蛋白质以及编码这些蛋白质的DNA序列。 这些蛋白质可以施用于家禽，从而保护鸟类免于球虫病。 此外，编码这些蛋白质的DNA可用于制备针对球虫病的载体疫苗。

公开(公告)号：US07211265B2

公开(公告)日：2007-05-01

申请号：US10841476

申请日：2004-05-10

申请人： David Grant Richards ,  Wayne Keith Jorgensen ,  Norman Porter Stewart

发明人： David Grant Richards ,  Wayne Keith Jorgensen ,  Norman Porter Stewart

IPC分类号： A61K39/012 ,  A61K39/00 ,  A01N63/00 ,  C12N1/00

CPC分类号： C12R1/01 ,  A61K39/00 ,  A61K39/012 ,  C07K14/455

摘要： Vaccines for the prevention and/or treatment of Eimeria infection including one or more strains of E. maxima ARI-73/97, E. acervulina ARI-77/97, E. tenella ARI-11/98, E. necatrix MCK01 and/or E. necatrix ARI-MEDNEC3+8 are described. Eimeria selected from E. maxima ARI-73/97, E. acervuina ARI-77/97, E. tenella ARI-11/98, E. necatrix MCK01 and/or E. mecatrix ARI-MEDNEC3+8 are also described.

摘要翻译： 用于预防和/或治疗艾美球虫感染的疫苗，包括一种或多种大肠杆菌ARI-73/97，，ina ina ina I I---------ella ella ella AR AR AR AR--rix rix rix rix rix / / 或E.necatrix ARI-MEDNEC 3 +8。 艾美球虫选自大肠杆菌ARI-73/97，大肠埃希氏菌属ARI-77/97，大肠杆菌ARI-11/98，大肠杆菌MCK01和/或E.mecatrix ARI-MEDNEC 3 > +8也被描述了。

公开(公告)号：US06998126B2

公开(公告)日：2006-02-14

申请号：US10371914

申请日：2003-02-21

申请人： Frans Gerrit Davelaar

发明人： Frans Gerrit Davelaar

IPC分类号： A61K39/012 ,  C12N1/36

CPC分类号： C12N1/36 ,  A61K39/012 ,  A61K2039/522

摘要： A method for obtaining an immunogenic strain useful for producing a vaccine against Coccidiosis comprises the cycle of infecting at least one first group of specific pathogen-free donor birds with oocysts from an Eimeria species. Blood is then collected from these donor birds, and is then used to infect a second group of specific pathogen-free birds. Oocysts are collected from the second group of birds. These oocysts are then multiplicated to complete the cycle. The cycle is then repeated using the multiplicated oocysts. After a total of about three cycles, a final antigen may be harvested and utilized as a source to generate oocysts for a vaccine.

公开(公告)号：US06984378B1

公开(公告)日：2006-01-10

申请号：US09701760

申请日：2000-02-25

申请人： Harold N. Conkle ,  Joseph E. Schultz ,  Scott J. Blonigen ,  Fred H. Weber ,  David R. Kilanowski ,  Bruce Monzyk ,  Timothy M. Werner ,  Chad M. Cucksey ,  Hamish A. I. McArthur ,  Ted L. Tewksbury

发明人： Harold N. Conkle ,  Joseph E. Schultz ,  Scott J. Blonigen ,  Fred H. Weber ,  David R. Kilanowski ,  Bruce Monzyk ,  Timothy M. Werner ,  Chad M. Cucksey ,  Hamish A. I. McArthur ,  Ted L. Tewksbury

IPC分类号： A61K63/00 ,  C12N1/00 ,  C12N1/10

CPC分类号： A61K39/012 ,  C12N1/10 ,  Y10S435/947

摘要： A vaccine for in ovo vaccination against avian coccidiosis produced by a method including obtaining the coccidial oocysts from a fecal suspension, homogenizing the fecal suspension, separating the oocysts from the fecal debris by either salt flotation using sodium sulfate or gas flotation using air, sporulating the oocysts using hydrogen peroxide and air sparging, bleaching the sporulated oocysts, washing the bleached oocysts, concentrating the sterile washed oocysts and combining the concentrates of various species of coccidial oocysts, and producing a vaccine. The method in whole or in part can be applied to other kinds of encysted protozoa to produce vaccines for various types of animals.

公开(公告)号：US06902926B1

公开(公告)日：2005-06-07

申请号：US10039770

申请日：2001-11-09

申请人： Gary E. Ward ,  Carolyn G. Conant ,  Brian Ward

发明人： Gary E. Ward ,  Carolyn G. Conant ,  Brian Ward

IPC分类号： A61K39/00 ,  A61K39/002 ,  A61K39/012 ,  C07K14/45 ,  C07K16/20 ,  C12N1/10 ,  C12Q1/68

CPC分类号： A61K39/012 ,  A61K39/00 ,  A61K39/002 ,  C07K14/45 ,  C07K16/20 ,  C07K2319/00

摘要： The invention provides polypeptide fragments derived from TgAMA-1, nucleic acids that encode the polypeptide fragments, and TgAMA-binding polypeptides such as antibodies. Methods for using the polypeptide and nucleic acid molecules to produce vaccines are also provided. In addition the invention provides methods involving use of the polypeptides, nucleic acids, and binding polypeptides, such as antibodies, for the prevention and treatment of Toxoplasmosis.