公开(公告)号：US20040084613A1

公开(公告)日：2004-05-06

申请号：US10274989

申请日：2002-10-22

发明人： Robert Harold Bateman ,  Martin Green ,  Michael Jackson

IPC分类号： H01J049/00

CPC分类号： H01J49/067 ,  H01J49/0027

摘要： A mass spectrometer is disclosed wherein a z-lens upstream of an orthogonal acceleration Time of Flight mass analyser is repeatedly switched between a first mode wherein ions are transmitted to the mass analyser for subsequent mass analysis with a relatively high transmission and a second mode wherein ions are transmitted with a relatively low transmission. If it is determined that mass spectral data obtained when the mass analyser is in the first mode is suffering from saturation, then suitably scaled mass spectral data obtained when the mass analyser is in the second mode is used instead. If the saturation is severe then the mass spectral data obtained in the first mode may be replaced in its entirety with mass spectral data obtained in the second mode.

摘要翻译： 公开了一种质谱仪，其中在正交加速时间飞行质量分析器上游的z轴透镜在第一模式之间重复切换，其中离子被传输到质量分析器以进行相对较高透射率的后续质量分析，其中离子 以较低的传输速率传输。 如果确定当质量分析器处于第一模式时获得的质谱数据饱和，则使用质量分析器处于第二模式时获得的适当缩放的质谱数据。 如果饱和度严重，则在第一模式中获得的质谱数据可以用第二模式获得的质谱数据全部替代。

公开(公告)号：US20040026615A1

公开(公告)日：2004-02-12

申请号：US10379379

申请日：2003-03-03

发明人： Richard N. Ellson ,  Mitchell W. Mutz ,  Richard Michael Caprioli

IPC分类号： H01J049/00

CPC分类号： H01J49/0418 ,  G01N33/54393 ,  G01N35/1011 ,  H01J49/0004 ,  H01J49/0454 ,  Y10T436/2575

摘要： Provided is a method for preparing a sample surface for analysis that involves placing a sample surface in droplet-receiving relationship to a reservoir containing an analysis-enhancing fluid. Typically, the analysis-enhancing fluid is comprised of a mass spectrometry matrix material and a carrier fluid, and the carrier fluid is comprised of a low volatility solvent. A droplet of the analysis-enhancing fluid from the reservoir such that the droplet is deposited on the sample surface at a designated site. Such ejection is typically, but not necessarily carried out through the application of focused acoustic energy. Then, the sample is subjected to conditions sufficient to allow the analysis-enhancing fluid to interact with the sample surface to render the sample surface suitable for analysis. Optionally, the sample is analyzed at the selected site. Also provided are systems and devices for preparing a sample surface for analysis.

摘要翻译： 提供了一种用于制备用于分析的样品表面的方法，其涉及将样品表面与包含分析增强流体的储液器的液滴接收关系放置。 通常，分析增强流体由质谱法基质材料和载体流体组成，并且载体流体由低挥发性溶剂组成。 来自储存器的分析增强流体的液滴使得液滴在指定位置沉积在样品表面上。 这种喷射通常但不一定通过应用聚焦声能来进行。 然后，样品经受足以使分析增强流体与样品表面相互作用以使样品表面适合分析的条件。 任选地，在所选择的位点分析样品。 还提供了用于制备用于分析的样品表面的系统和装置。

公开(公告)号：US20040021069A1

公开(公告)日：2004-02-05

申请号：US10421676

申请日：2003-04-23

申请人： Thermo Electron Corporation

发明人： Bryan Robert Barnard

IPC分类号： H01J049/00 ,  B01D059/44

CPC分类号： H01J49/40

摘要： A spectroscopic analyser and method of use, for surface analysis spectroscopy, are disclosed. The spectroscopic analyser 10 has a time-of-flight (TOF) spectrometer which analyses secondary electrons emitted from a surface of a sample 30 on excitation by an irradiation source 40. The TOF spectrometer includes a gate 50, which receives and selectively passes a proportion of the secondary electrons by pulsed deflection or retardation of the electron beam using gating members 55. In that manner one or more pulses of electrons enter a magnetic field-free flight tube 90 and reach a detector 120 downstream of the gate 50. The flight times, and therefore energies, of the detected electrons through the flight tube 90 are thereby detected. A curved electron mirror 100 may be used to increase the flight path of the pulsed electrons in the flight tube 90, thereby increasing the spread of each electron pulse within the analyser 10.

摘要翻译： 公开了用于表面分析光谱的光谱分析仪和使用方法。 光谱分析仪10具有飞行时间（TOF）光谱仪，其分析由照射源40激发时从样品30的表面发射的二次电子。TOF光谱仪包括门50，其接收并选择性地通过比例 的二次电子通过使用门控构件55的电子束的脉冲偏转或延迟。以这种方式，一个或多个电子脉冲进入无磁场飞行管90并到达门50下游的检测器120.飞行时间 ，因此检测到通过飞行管90检测到的电子的能量。 可以使用弯曲电子反射镜100来增加飞行管90中的脉冲电子的飞行路径，从而增加分析器10内的每个电子脉冲的扩展。

公开(公告)号：US20030226963A1

公开(公告)日：2003-12-11

申请号：US10335007

申请日：2002-12-31

发明人： Robert G. Cooks ,  Zheng Ouyang

IPC分类号： H01J049/00

CPC分类号： H01J49/04 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/25875

摘要： A method of separating species in a mixture of molecules, particles or atoms and collecting the separated species is described. The method comprises the steps of converting by ionization the species in the mixture to gas phase ions, separating the gas phase ions according to their mass charge ratio and/or mobility and collecting the separated ions. The system includes ionizing means such as electrospray to form the gas phase ions. The gas phase ions are separated by filtering, or in time or in space and soft-landed for collection such as on a surface.

摘要翻译： 描述了在分子，颗粒或原子的混合物中分离物质并收集分离的物质的方法。 该方法包括以下步骤：通过电离将混合物中的物质转化为气相离子，根据其质荷比和/或迁移率分离气相离子并收集分离的离子。 该系统包括电离装置，例如电喷雾以形成气相离子。 气相离子通过过滤，或在时间上或在空间中分离，并且软着陆以用于收集，例如在表面上。

公开(公告)号：US20030201390A1

公开(公告)日：2003-10-30

申请号：US10405689

申请日：2003-04-01

发明人： Thomas N. Corso ,  Gary A. Schultz ,  Simon J. Prosser ,  Xian Huang

IPC分类号： H01J049/00 ,  B01D059/44

CPC分类号： G01N30/466 ,  B01J20/261 ,  B01J20/267 ,  B01J20/281 ,  B01J20/285 ,  B01J2220/54 ,  B01J2220/82 ,  G01N27/44717 ,  G01N30/463 ,  G01N30/467 ,  G01N30/48 ,  G01N30/6095 ,  G01N30/7266 ,  G01N2030/285 ,  G01N2030/528 ,  H01J49/0018 ,  H01J49/167

摘要： A microfabricated silicon chip with a separation material, such as in situ prepared porous polymer monoliths in its microchannels is disclosed. The polymer monoliths are liquid-permeable and serve as microcolumns for liquid chromatography, which are prepared by in situ radical polymerization of a mixture containing vinyl monomers and solvents (porogen) in the microchannels. A method and system are disclosed to generate one or more electrospray plumes from one or more nozzles that provide an ion intensity as measured by a mass spectrometer that is approximately proportional to the number of electrospray plumes formed for analyses contained within the fluid. A plurality of electrospray devices can be used in the form of an array of miniaturized separate electrospray devices for the purpose of generating multiple electrospray plumes from multiple nozzles for the same fluid for analysis. This invention dramatically increases the sensitivity of microchip electrospray devices compared to prior disclosed systems and methods. The silicon chip having the packed microchannels disclosed herein finds application in coupling with mass spectrometry for sample analysis. Also disclosed is a separation block having multiple through-substrate channels filled with a separation material such as polymer monolith which can be stacked in multiple blocks for sequential two-dimensional chromatographic separation and integrated with the electrospray device.

公开(公告)号：US20030178561A1

公开(公告)日：2003-09-25

申请号：US10380248

申请日：2003-03-11

发明人： Tokudia Neda ,  Yasuharu Yokoi ,  Kenji Maeda ,  Kenji Horiuchi ,  Satoshi Yamashita

IPC分类号： H01J049/00

CPC分类号： G01N21/3563 ,  G01N21/65 ,  G06K7/10 ,  G06K19/06 ,  G07D7/1205

摘要： A nondestructive reading method for an isotopic label, capable of easily and quickly reading information on an isotopic label imparted to an article in advance without destroying the label, and a nondestructive reading-use isotopic label suitably using this method. In addition, information obtained by this nondestructive reading method is used to easily and quickly judge the authenticity of an article.

摘要翻译： 用于同位素标签的非破坏性阅读方法，能够容易且快速地读取预先赋予物品的同位素标签上的信息而不破坏标签，以及适当地使用该方法的非破坏性阅读用同位素标签。 另外，通过这种非破坏性阅读方法获得的信息用于容易且快速地判断物品的真实性。

公开(公告)号：US20030164449A1

公开(公告)日：2003-09-04

申请号：US10296381

申请日：2002-11-25

发明人： Elmar Heinzle ,  Min-Jung Kang ,  Andreas Tholey ,  Klaus Hollemeyer ,  Bernhard Hauer

IPC分类号： H01J049/00 ,  B01D059/44

CPC分类号： H01J49/04

摘要： A process is described for analyzing enzyme-catalyzed conversions of nonpolymeric substrates to nonpolymeric products with the aid of MALDI-TOF mass spectrometry, preferably in the presence of an internal standard on a specific carrier material.

摘要翻译： 描述了一种方法，用于借助MALDI-TOF质谱法，优选在特定载体材料上存在内标物的情况下分析非聚合物底物对非聚合物产物的酶催化转化。

公开(公告)号：US20030155506A1

公开(公告)日：2003-08-21

申请号：US10295010

申请日：2002-11-14

发明人： Viatcheslav S. Motchkine ,  Leonid Y. Krasnobaev ,  Stephen N. Bunker

IPC分类号： H01J049/00 ,  B01D059/44

CPC分类号： G01N1/02 ,  G01N2001/022 ,  G01N2001/028

摘要： The presence of trace molecules in air may be determined using an ion mobility spectrometer. Such devices may be used in the fields of explosives detection, identification of narcotics, and in applications characterized by the presence of very low airborne concentrations of organic molecules of special interest. The sensitivity of such instruments is dependent on the method of gas sampling utilized. A cyclone sampling nozzle can greatly improve the sampling efficiency, particularly when the sampling needs to be performed at a distance from the air intake. The cyclone sampling nozzle consists of an intake gas flow and a separate coaxial emitted gas flow which is deflected to move with a circular motion.

摘要翻译： 可以使用离子迁移谱仪测定空气中微量分子的存在。 这种装置可用于爆炸物检测，麻醉药品鉴定以及特别是存在非常低空气浓度的特别有趣的有机分子的应用。 这些仪器的灵敏度取决于所采用的气体采样方法。 旋风采样喷嘴可以大大提高采样效率，特别是当需要在与进气口相距一定距离进行采样时。 旋风采样喷嘴由进气气流和单独的同轴排出的气流组成，其被偏转以便以圆周运动移动。

公开(公告)号：US20030153007A1

公开(公告)日：2003-08-14

申请号：US10330861

申请日：2002-12-26

发明人： Jian Chen ,  Joseph Michel Daniel Figeys ,  Brett Larsen ,  Forest M. White

IPC分类号： G01N033/53 ,  B01D059/44 ,  H01J049/00

CPC分类号： G01N33/6848 ,  C12Q1/485 ,  G01N33/6803 ,  G01N33/6812 ,  G01N33/6818 ,  G01N33/6821 ,  G01N33/6842

摘要： Methods and systems of applying mass spectrometry to the analysis of peptides and amino acids, especially in the proteome setting. More particularly, the invention relates to a mass spectrometry-based method for detection of amino acid modifications, such as phosphorylation.

摘要翻译： 应用质谱法分析肽和氨基酸，特别是蛋白质组的方法和系统。 更具体地，本发明涉及用于检测氨基酸修饰如磷酸化的基于质谱法的方法。

公开(公告)号：US20030132377A1

公开(公告)日：2003-07-17

申请号：US10301710

申请日：2002-11-22

发明人： Robert Harold Bateman ,  Jeff Brown ,  Anthony James Gilbert

IPC分类号： H01J049/00

CPC分类号： H01J49/004 ,  H01J49/401 ,  H01J49/42

摘要： A mass spectrometer is disclosed comprising a mass selective ion trap such as a 3D quadrupole field ion trap upstream of a pusher electrode of an orthogonal acceleration Time of Flight mass analyser. According to a first embodiment bunches of ions are released from the ion trap and the pusher electrode is energised after a delay time which is progressively varied. According to a second embodiment ions are released from the ion trap in reverse order of mass to charge ratio with the ions having the largest mass to charge ratio being released first. By appropriate release of the ions from the ion trap it is possible to ensure that substantially all of the ions arrive at the pusher electrode at substantially the same time. According to both embodiments it is possible to achieve a duty cycle approaching 100% across a large range of mass to charge ratios.

摘要翻译： 公开了一种质谱仪，其包括质量选择性离子阱，例如正交加速时间飞行质量分析仪的推进器电极上游的3D四极场离子阱。 根据第一实施例，一束离子从离子阱中释放出来，并且推进器电极在逐渐变化的延迟时间之后通电。 根据第二实施方案，离子从离子阱以与质荷比最大的离子的质荷比相反的顺序被释放。 通过从离子阱适当释放离子，可以确保基本上所有的离子基本上同时到达推动器电极。 根据两个实施例，可以实现在大范围的质荷比之间接近100％的占空比。