公开(公告)号：US20080085506A1

公开(公告)日：2008-04-10

申请号：US11641343

申请日：2006-12-19

申请人： Bryan Bjorndal ,  David Lapota

发明人： Bryan Bjorndal ,  David Lapota

IPC分类号： C12Q1/00 ,  C12M3/00

CPC分类号： G01N21/6486 ,  G01N21/763 ,  G01N33/1866 ,  G01N33/5085 ,  G01N33/54366

摘要： A system for testing the toxicity of a test sample comprising: an aqueous suspension of dinoflagellates; a test chamber configured to contain the aqueous suspension of dinoflagellates and the test sample; an optical signal generator configured to emit an excitation signal for exciting the dinoflagellates to emit a fluorescence signal if the dinoflagellates are alive; a first optical transducer configured to produce a first data signal in response to detecting the fluorescence signal; a stimulator configured to introduce a gas into the aqueous suspension for stimulating the dinoflagellates to emit a bioluminescence signal if the dinoflagellates are alive; a second optical transducer configured to produce a second data signal in response to detecting the bioluminescence signal; and a processor configured to compare the first data signal and the second data signal to a control data to generate an output representing the toxicity of the test sample.

摘要翻译： 一种用于测试测试样品的毒性的系统，包括：鞭毛藻的水性悬浮液; 测试室，被配置为容纳鞭毛藻的水性悬浮液和测试样品; 光信号发生器，其被配置为发射激发信号，用于激发所述鞭毛藻，如果所述鞭毛藻是活的，则发出荧光信号; 配置为响应于检测到所述荧光信号而产生第一数据信号的第一光学换能器; 刺激器，其被配置为将气体引入所述水性悬浮液中以刺激所述鞭毛藻发出生物发光信号，如果所述鞭毛藻是活的; 第二光学换能器，被配置为响应于检测到所述生物发光信号产生第二数据信号; 以及处理器，被配置为将所述第一数据信号和所述第二数据信号与控制数据进行比较，以产生表示所述测试样品的毒性的输出。

公开(公告)号：US06737263B2

公开(公告)日：2004-05-18

申请号：US09940298

申请日：2001-08-28

申请人： Dominic A. Dragotta ,  Vasantha Nagarajan ,  Stuart M. Thomas

发明人： Dominic A. Dragotta ,  Vasantha Nagarajan ,  Stuart M. Thomas

IPC分类号： B09B300

CPC分类号： G01N33/1866 ,  C02F3/006 ,  C12Q1/045 ,  G01N33/64 ,  G01N33/66 ,  G01N2333/195 ,  G01N2333/21 ,  G01N2333/22 ,  G01N2333/225 ,  G01N2333/355 ,  G01N2800/52

摘要： A method has been developed to monitor the health of an activated sludge environment in a wastewater process comprising monitoring the levels of polyhydroxyalkanoates (PHA) produced and correlating those levels with various selected sample parameters. In general, levels of PHA in excess of about 15% to about 20% dry weight of the biomass is an indication that the biocatalytic efficiency of the wastewater treatment process is impaired.

摘要翻译： 已经开发了一种监测废水处理过程中活性污泥环境的健康状况的方法，包括监测产生的聚羟基链烷酸酯（PHA）的含量，并将这些水平与各种选定的样品参数相关联。 通常，超过生物质干重的约15％至约20％的PHA水平表明废水处理过程的生物催化效率受损。

公开(公告)号：US06710871B1

公开(公告)日：2004-03-23

申请号：US09600021

申请日：2000-07-07

申请人： Philippe J. Goix

发明人： Philippe J. Goix

IPC分类号： G01N2164

CPC分类号： G01N15/1459 ,  C12Q1/04 ,  G01N21/05 ,  G01N33/1866 ,  G01N2015/1477 ,  G01N2015/1486 ,  G01N2021/0346 ,  G01N2021/6419 ,  G01N2021/6421 ,  G02B2207/113

摘要： A method and apparatus for detecting a fluorescent substance tagged to a microparticle are described. The device comprises a single capillary flow carrier system for transporting the microparticle past a selected location, a source of electromagnetic radiation for irradiating the substance tagged to the microparticle, and a detection system for measuring fluorescent light emitted from the substance at the selected location. The method comprises transporting the microparticle to a selected location, irradiating a fluorescent substance tagged to the microparticle, and measuring the fluorescent light emitted from the fluorescent substance at the selected location.

公开(公告)号：US06451212B2

公开(公告)日：2002-09-17

申请号：US09947469

申请日：2001-09-07

申请人： Hajime Iseri ,  Yutaka Yoneda ,  Kuniyuki Takahashi

发明人： Hajime Iseri ,  Yutaka Yoneda ,  Kuniyuki Takahashi

IPC分类号： C02F150

CPC分类号： G01N33/1866 ,  C02F1/008 ,  C02F1/50 ,  C02F2103/023 ,  C02F2209/04 ,  G01N17/008

摘要： According to the method of treating water in a cooling water system, adhesion of fouling is on-line monitored by a compact and inexpensive apparatus and a slime control treatment is intensified according to the result of on-line monitoring. The water treatment is intensified in response to the change of electric potential of a sensor monitoring microbial fouling 11 made of sensitized metallic material. When the electric potential of the sensor exceeds a threshold value, an agent is added through both chemical feeding pumps 15 and 17. When the electric potential lowers to the normal value, the agent is added only through the pump 15.

摘要翻译： 根据在冷却水系统中处理水的方法，通过紧凑廉价的装置在线监测污垢粘附，并且根据在线监测的结果增强了粘液控制处理。 响应于由感光金属材料制成的传感器监测微生物污垢11的电位变化，水处理增强。 当传感器的电位超过阈值时，通过化学供给泵15和17两者添加试剂。当电位降低到正常值时，仅通过泵15添加试剂。

公开(公告)号：US06436698B2

公开(公告)日：2002-08-20

申请号：US09761654

申请日：2001-01-18

申请人： Han Oh Park ,  Hanee Park ,  Il Kyu Choi

发明人： Han Oh Park ,  Hanee Park ,  Il Kyu Choi

IPC分类号： C12M136

CPC分类号： C12Q1/02 ,  G01N21/763 ,  G01N33/1826 ,  G01N33/1866 ,  G01N2520/00 ,  Y02A20/206

摘要： The present invention relates to an apparatus for automatic measuring of water toxicity. More specifically, the present invention provides an apparatus for automatic and continuous measuring of water toxicity and/or contamination using luminescent microorganisms living in freshwater.

摘要翻译： 本发明涉及一种用于自动测量水毒性的装置。 更具体地说，本发明提供一种用于利用居住在淡水中的发光微生物自动连续地测量水毒性和/或污染的装置。

公开(公告)号：US06416994B1

公开(公告)日：2002-07-09

申请号：US09485953

申请日：2000-02-17

申请人： Akira Uruga ,  Seiji Imabayashi ,  Yasushi Kitagawa ,  Yoshmitsu Harada

发明人： Akira Uruga ,  Seiji Imabayashi ,  Yasushi Kitagawa ,  Yoshmitsu Harada

IPC分类号： C12M134

CPC分类号： C12M21/04 ,  C02F3/28 ,  C12M41/36 ,  G01N33/1866 ,  Y02E50/343

摘要： The present invention provides a specific methanogenic activity measuring apparatus, in which a carbon dioxide gas absorbing container 6 is removably accommodated in a pressure vessel 4 with a cover 3 removably set thereon, the pressure vessel 4 is removably accommodated in a thermostatic water bath 7 with a pressure transducer 8 based on the pressure-electricity converting system connected to a recorder 5 attached to the cover 3, and further a supporting member for supporting a carbon dioxide gas absorbing container and a generated gas piping 16 are attached to an external bottom surface and an internal bottom surface of the carbon dioxide gas absorbing container 6 respectively, and gas piping 12 with valves for gas phase replacement to the cover 3 of the pressure vessel 4.

摘要翻译： 本发明提供了一种特定的产甲烷活性测定装置，其中二氧化碳气体吸收容器6可移除地容纳在压力容器4中，其中可拆卸地设置有盖3，压力容器4可移除地容纳在具有 基于连接到附接到盖3的记录器5的压力 - 电力转换系统的压力传感器8，以及用于支撑二氧化碳气体吸收容器和产生的气体配管16的支撑构件附接到外部底面， 分别为二氧化碳气体吸收容器6的内部底面，以及具有用于气相置换的压力容器4的盖3的气体配管12。

公开(公告)号：US20020060020A1

公开(公告)日：2002-05-23

申请号：US09902248

申请日：2001-07-10

申请人： Hercules Incorporated

发明人： Richard M. Irwin ,  Geary G. Yee

IPC分类号： D21F007/00 ,  D21F007/06

CPC分类号： G01N17/008 ,  G01N21/552 ,  G01N33/18 ,  G01N33/1866 ,  G01N33/36

摘要： Online methods of quantitatively and qualitatively monitoring the biofilm and deposition organic and inorganic contaminants in paper processing equipment provided. Spectroscopic methods, and more specifically attenuated total reflectance techniques are disclosed.

摘要翻译： 在线提供的纸张加工设备中定量和定性监测生物膜和沉积有机和无机污染物的在线方法。 公开了光谱方法，更具体地说是衰减的全反射技术。

公开(公告)号：US6110661A

公开(公告)日：2000-08-29

申请号：US944934

申请日：1997-10-02

申请人： Curtis A. Lajoie ,  Christine Jo Kelly ,  Alice C. Layton ,  Gary S. Sayler

发明人： Curtis A. Lajoie ,  Christine Jo Kelly ,  Alice C. Layton ,  Gary S. Sayler

IPC分类号： C12N15/09 ,  C02F3/12 ,  C12M1/34 ,  C12M1/36 ,  C12N20060101 ,  C12N1/21 ,  C12N15/53 ,  C12N15/74 ,  C12Q20060101 ,  C12Q1/66 ,  C12R1/01 ,  G01N33/18 ,  C12Q1/00 ,  C12Q1/02

CPC分类号： G01N33/1866 ,  C12N15/74

摘要： The present invention provides a reporter bacterium, comprising a bacterium that occurs naturally in a biological sludge and that contains a nucleic acid that encodes a reporter protein not found in the naturally occurring bacterium. The nucleic acid can encode a bioluminescent reporter protein. A method and apparatus are also provided for detecting the presence of toxicity in a wastewater treatment influent stream, comprising contacting the influent with a reporter bacterium of the present invention; monitoring the expression of the reporter protein by the reporter bacterium; and correlating a reduction in the expression of the reporter protein with the presence of toxicity. In an additional embodiment, the present invention provides a method and apparatus for determining the effect of a known dose of an oxidative phosphorylation decoupling agent on biological wastewater sludge, comprising contacting a sample of the sludge containing a known dose of the decoupling agent and a control sample, with a reporter bacterium of the present invention; comparing the signal output by the reporter bacteria in the sludge sample containing the decoupling agent with the signal output by the reporter bacteria in the sludge sample not containing the decoupling agent; and correlating a decrease in signal output in the decoupled sample as compared to the signal output in the control sample with an action of the decoupling agent, whereby the effect of the known dose of the decoupling agent is determined.

摘要翻译： 本发明提供了一种报道细菌，其包含在生物污泥中天然存在并含有编码天然存在细菌中未报道的报告基因蛋白的核酸的细菌。 核酸可编码生物发光报道蛋白。 还提供了一种用于检测废水处理流入物流中毒性的存在的方法和装置，包括使流入物与本发明的报道细菌接触; 通过报道细菌监测报道蛋白的表达; 并将报告蛋白的表达降低与毒性的存在相关联。 在另外的实施方案中，本发明提供了一种用于确定已知剂量的氧化磷酸化去耦剂对生物废水污泥的影响的方法和装置，包括使含有已知剂量的去耦剂的污泥样品和对照 采用本发明的报道细菌; 将含有去耦剂的污泥样品中的报道细菌的信号输出与不含去耦剂的污泥样品中报道细菌的信号输出进行比较; 并且与解耦剂的作用相比，在解耦样品中的信号输出的降低与对照样品中的信号输出相关，从而确定去耦剂的已知剂量的影响。

公开(公告)号：US6054030A

公开(公告)日：2000-04-25

申请号：US873590

申请日：1997-06-12

申请人： Cristiani Pierangela ,  Mollica Alfonso ,  Ventura Giovanna

发明人： Cristiani Pierangela ,  Mollica Alfonso ,  Ventura Giovanna

IPC分类号： G01N27/416 ,  G01N33/18 ,  G01N27/26

CPC分类号： G01N33/1866 ,  G01N27/4168 ,  G01N2033/184

摘要： A probe is conveniently positioned in a flowing aqueous medium. The probe is formed by two electrodes of notable difference in nobility connected by an electric resistance high enough to ensure that the voltage difference between its terminals is sufficiently great to permit accurate and simultaneous determinations of the growth of biofouling and the chlorine concentration by measuring the potential difference variations between the terminals.

摘要翻译： 探针方便地位于流动的水性介质中。 探头由两个电极形成，两个电极具有显着的差异性，通过电阻高达足够高的电阻，以确保其端子之间的电压差足够大，以允许通过测量电位来准确和同时地测定生物污垢的生长和氯浓度 端子之间的差异变化。

公开(公告)号：US6053032A

公开(公告)日：2000-04-25

申请号：US9968

申请日：1998-01-21

申请人： Paul R. Kraus ,  Jeffrey P. Chamberlain ,  Linda R. Robertson ,  Barbara E. Moriarty

发明人： Paul R. Kraus ,  Jeffrey P. Chamberlain ,  Linda R. Robertson ,  Barbara E. Moriarty

IPC分类号： G01N17/00 ,  G01N27/00 ,  G01N33/18 ,  G01N33/00

CPC分类号： G01N29/036 ,  G01N17/008 ,  G01N33/1866 ,  G01N2033/184 ,  G01N2291/0256 ,  G01N33/1853

摘要： A system and a method are provided for detecting and monitoring changes in frequency in a process stream. At least one probe is placed in the process stream that is capable of detecting a change in frequency due to a build-up of mass on the probe from scale, corrosion, biofilm or the like in the process stream. A processor receives the signal produced by the probe to produce an output signal indicative of the change in frequency which is proportional to mass detected by the probe. A feeder is capable of feeding a product into the process stream as a result of the detected change in frequency of the probe to combat the mass build-up from scale, corrosion, biofilm or the like in the process stream. The system and method are further capable of measuring anti-scalant, corrosion inhibitor, biocide efficacy and controlling product feed into the process stream based on the determined efficacy.

摘要翻译： 提供了一种用于检测和监视处理流中的频率变化的系统和方法。 至少一个探针被放置在工艺流中，其能够检测由于在工艺流中的氧化皮，腐蚀，生物膜等在探针上的质量的积聚而引起的频率变化。 处理器接收由探头产生的信号，以产生指示频率变化的输出信号，其与由探针检测到的质量成比例。 由于检测到的探针变化频率的变化，进料器能够将产物输送到工艺流中，以防止在工艺流中的垢，腐蚀，生物膜等的质量堆积。 该系统和方法还能够基于确定的功效测量抗鳞屑，腐蚀抑制剂，杀生物剂功效并且控制产物进料到工艺流中。