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    2,5-diketo-D-gluconic acid (2,5-DKG) permeases
    75.
    发明授权
    2,5-diketo-D-gluconic acid (2,5-DKG) permeases 有权
    2,5-二酮-D-葡萄糖酸（2,5-DKG）渗透酶

    公开(公告)号：US07229811B2

    公开(公告)日：2007-06-12

    申请号：US10343369

    申请日：2001-08-03

    Applicant: Manoj Kumar ,  Fernando Valle ,  Veronique A. Dartois ,  James A. Hoch

    Inventor： Manoj Kumar ,  Fernando Valle ,  Veronique A. Dartois ,  James A. Hoch

    IPC: C07H21/04 ,  C12N15/00 ,  C12N1/20 ,  C12P7/40 ,  C12P21/06 ,  C12Q1/26 ,  C07K14/00 ,  C12P19/34 ,  C12N9/04

    CPC classification number: C07K14/26 ,  C07K14/24

    Abstract: The invention provides isolated nucleic acid molecules encoding polypeptides having 2,5-DKG permease activity, and oligonucleotides therefrom. The isolated nucleic acid molecules can be expressed in appropriate bacterial cells to enhance the production of 2-KLG, which can subsequently be converted to ascorbic acid. Further provided are isolated polypeptides having 2,5-DKG permease acitivity, immunogenic peptides therefrom, and antibodies specific therefor. The invention also provides methods of identifying novel 2,5-DKG permeases.

    Abstract translation: 本发明提供了编码具有2,5-DKG通透酶活性的多肽的分离的核酸分子及其寡核苷酸。 分离的核酸分子可以在适当的细菌细胞中表达以增强2-KLG的产生，随后将其转化为抗坏血酸。 还提供了具有2,5-DKG通透酶活性的分离的多肽，其免疫原性肽及其特异的抗体。 本发明还提供鉴定新型2,5-DKG通透酶的方法。

    Method for the production of 1,3-propanediol by recombinant organisms comprising genes for coenzyme B12 synthesis
    76.
    发明授权
    Method for the production of 1,3-propanediol by recombinant organisms comprising genes for coenzyme B12 synthesis 有权
    用于辅酶B12合成基因的重组生物制备1,3-丙二醇的方法

    公开(公告)号：US07074608B1

    公开(公告)日：2006-07-11

    申请号：US09310001

    申请日：1999-05-11

    Applicant: Nigel S. Dunn-Coleman ,  Anthony A. Gatenby ,  Fernando Valle

    Inventor： Nigel S. Dunn-Coleman ,  Anthony A. Gatenby ,  Fernando Valle

    IPC: C12N1/20

    CPC classification number: C12N9/88 ,  C12P7/18 ,  C12Y402/0103

    Abstract: Recombinant organisms are provided comprising genes encoding aquacobalamin reductase, cob(II)alamin reductase, cob(I)alamin adenosyltransferase, glycerol dehydratase and 1,3-propanediol oxidoreductase activities useful for the production of 1,3-propanediol from a variety of carbon substrates. More specifically the following nucleotide sequences are provided: btuR, encoding the E. coli cob(I)alamin adenosyltransferase enzyme; cobA, encoding the Salmonella typhimurium cob(I)alamin adenosyltransferase enzyme; cobO, encoding the Pseudomonas denitrificans cob(I)alamin adenosyltransferase enzyme; dhaB1, encoding the α subunit of the Klebsiella glycerol dehydratase enzyme; dhaB2, encoding the β subunit of the Klebsiella glycerol dehydratase enzyme; dhaB3, encoding the γ subunit of the Klebsiella glycerol dehydratase enzyme; dhaT, encoding Klebsiella oxidoreductase enzyme; the yciK gene isolated from E. coli; the glucose isomerase promoter sequence from Streptomyces; and the N-terminal amino acid sequence for cob(II)alamin reductase from Pseudomonas denitrificans.

    Abstract translation: 提供重组生物，其包含编码水产精氨酸还原酶，cob（II）氨基还原酶，cob（I）阿拉曼腺苷转移酶，甘油脱水酶和1,3-丙二醇氧化还原酶活性的基因，所述活性可用于从各种碳底物生产1,3-丙二醇 。 更具体地提供以下核苷酸序列：btuR，编码大肠杆菌（I）氨基腺苷酰基转移酶; cobA，编码鼠伤寒沙门氏菌（I）阿拉明腺苷转移酶; 编码脱氧核糖核酸假单胞菌（I）氨基腺苷酰基转移酶的cobO; dhaB1，编码克雷伯杆菌甘油脱水酶的α亚基; dhaB2，编码克雷伯氏菌甘油脱水酶的β亚基; dhaB3，编码克雷伯菌甘油脱水酶的γ亚基; dhaT，编码克雷伯杆菌氧化还原酶; 从大肠杆菌分离的yciK基因; 来自链霉菌的葡萄糖异构酶启动子序列; 和来自脱氮假单胞菌的cob（II）氨基还原酶的N-末端氨基酸序列。

    Methods for the production of products in host cells
    77.
    发明授权
    Methods for the production of products in host cells 有权

    公开(公告)号：US07033804B2

    公开(公告)日：2006-04-25

    申请号：US10116821

    申请日：2002-04-04

    Applicant: Timothy C. Dodge ,  Fernando Valle

    Inventor： Timothy C. Dodge ,  Fernando Valle

    IPC: C12P7/60 ,  C12P7/58

    CPC classification number: C12N15/52 ,  C12P7/46 ,  C12P7/60 ,  C12P19/02

    Abstract: The invention provides methods and host cells for the production of ascorbic acid intermediates. The invention also provides host cells having a modification in a polynucleotide that uncouples the catabolic pathway from the oxidative pathway by deleting the encoding for an endogenous enzymatic activity that phosphorylates D-glucose at its 6th carbon and/or a polynucleotide that has deleted the encoding for endogenous enzymatic activity that phosphorylates D-gluconate at its 6th carbon. Such host cells are used for the production of products, such as, ascorbic acid intermediates. Nucleic acid and amino acid sequences with inactivated enzymatic activity which phosphorylates D-glucose at its 6th carbon and inactivated enzymatic activity which phosphorylates D-gluconate at its 6th carbon are provided.

    Application of glucose transport mutants for production of aromatic pathway compounds
    78.
    发明授权
    Application of glucose transport mutants for production of aromatic pathway compounds 失效
    葡萄糖转运突变体用于生产芳香途径化合物的应用

    公开(公告)号：US06962794B2

    公开(公告)日：2005-11-08

    申请号：US08940692

    申请日：1997-09-30

    Applicant: Fernando Valle ,  Noemi Mejia ,  Alan Berry

    Inventor： Fernando Valle ,  Noemi Mejia ,  Alan Berry

    IPC: C12N1/20 ,  C12N1/21 ,  C12N15/52 ,  C12P1/00 ,  C12P13/22 ,  C12P19/04 ,  C12N1/00 ,  C12N15/01 ,  C12N15/09

    CPC classification number: C12P13/22 ,  C12N1/20 ,  C12N15/52 ,  C12P1/00 ,  Y10S435/813

    Abstract: This invention describes methods for enhancing carbon flow into a pathway of a host cell to enhance the biosynthetic production of compounds therefrom, the host cells being selected based on being phenotypically Pts−/glucose+. Such host cells are capable of transporting glucose without consuming PEP, resulting in conservation of PEP which can be re-directed into the pathway in order to enhance the production of desired compounds along the pathway. Pts−/glucose+ mutants have been shown to be advantageous for the enhanced production of the aromatic amino acids.

    Abstract translation: 本发明描述了用于增强进入宿主细胞途径的碳流动以增强其化合物的生物合成生产的方法，基于表型为基因表达来选择宿主细胞/ SUP>。 这样的宿主细胞能够在不消耗PEP的情况下运输葡萄糖，导致PEP的保护，其可被重新引导到途径中，以便增强沿着途径产生所需化合物。 已显示Pts /葡萄糖+突变体对于增强芳族氨基酸的产生是有利的。

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