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102 条记录 (耗时 0.03 秒)

    Determination of free thyroid hormones by competitive immunoassay
    72.
    发明授权
    Determination of free thyroid hormones by competitive immunoassay 失效
    通过竞争性免疫测定法测定游离甲状腺激素

    公开(公告)号：US5639670A

    公开(公告)日：1997-06-17

    申请号：US325409

    申请日：1995-01-17

    申请人： Andreas Bergmann ,  Joachim Struck

    发明人： Andreas Bergmann ,  Joachim Struck

    IPC分类号： G01N33/53 ,  G01N33/543 ,  G01N33/58 ,  G01N33/68 ,  G01N33/78

    CPC分类号： G01N33/78 ,  G01N33/54306 ,  G01N33/58 ,  G01N33/686 ,  Y10S435/961 ,  Y10S435/962 ,  Y10S435/968 ,  Y10S435/971 ,  Y10S435/975 ,  Y10S436/808 ,  Y10S436/817 ,  Y10S436/822 ,  Y10S436/824

    摘要： A method is revealed for the quantitative determination of the proportion of the free form of a thyroid hormone ligand in a sample of a biological fluid in which the ligand is present partly in the free form and also partly in a form in which it is bound to physiological binding proteins. In the first step, a ligand derivative of the thyroid hormone with an immunoglobulin, a labelled specific binder, and a test tube whose walls are coated with an excess of a protein material are provided. In the second step, a sample of the biological fluid containing an unknown amount of the free thyroid hormone ligand, a solution containing a known amount of the ligand derivative, and a solution containing a known, less than stoichiometric amount of the labelled specific binder are added to the test tube to form a liquid reaction mixture. In the third step, the reaction mixture is incubated to allow the free thyroid hormone ligand and the ligand derivative in the liquid reaction mixture to compete for the binding sites of the labelled specific binder, and to allow, in a concomitant slower reaction, the binding of the immunoglobulin part of the ligand derivative to the excess of the protein material on the walls of the test tube. In the fourth step, the remaining liquid reaction mixture is removed from the test tube. In the final steps, the amount of labelled specific binder bound to the walls of the test tube is determined and the amount of the free thyroid hormone ligand calculated.

    摘要翻译： PCT No.PCT / EP93 / 00981 Sec。 371日期1995年1月17日 102（e）日期1995年1月17日PCT提交1993年4月22日PCT公布。 公开号WO93 / 22675 日期1993年11月11日揭示了一种生物流体样品中游离形式的甲状腺激素配体的比例的定量测定方法，其中配体部分以游离形式存在，并且部分以 它与生理结合蛋白结合。 在第一步中，提供具有免疫球蛋白的甲状腺激素的配体衍生物，标记的特异性结合剂和其壁用过量的蛋白质材料包被的试管。 在第二步中，含有未知量的游离甲状腺激素配体，含有已知量的配体衍生物的溶液和含有已知的少于化学计量的标记的特异性粘合剂的溶液的生物流体样品是 加入到试管中以形成液体反应混合物。 在第三步骤中，将反应混合物温育以允许液体反应混合物中的游离甲状腺激素配体和配体衍生物竞争标记的特异性结合物的结合位点，并允许伴随较慢反应的结合 的配体衍生物的免疫球蛋白部分与试管壁上的蛋白质材料的过量。 在第四步骤中，将剩余的液体反应混合物从试管中取出。 在最后的步骤中，确定与试管壁结合的标记的特异性结合物的量，计算游离甲状腺激素配体的量。

    VASOACTIVE HORMONE-BASED STRATIFICATION OF PATIENTS SUFFERING FROM DISEASES RELATED TO ENDOTHELIAL FUNCTION/DYSFUNCTION
    76.
    发明申请
    VASOACTIVE HORMONE-BASED STRATIFICATION OF PATIENTS SUFFERING FROM DISEASES RELATED TO ENDOTHELIAL FUNCTION/DYSFUNCTION 有权
    基于HOMMONE的基于HORMONE的分离与疾病相关的患者与内分泌功能/功能丧失

    公开(公告)号：US20120142120A1

    公开(公告)日：2012-06-07

    申请号：US13318488

    申请日：2010-05-05

    申请人： Andreas Bergmann ,  Joachim Struck ,  Oliver Hartmann ,  Leong Loke Ng

    发明人： Andreas Bergmann ,  Joachim Struck ,  Oliver Hartmann ,  Leong Loke Ng

    IPC分类号： G01N33/74

    CPC分类号： G01N33/74 ,  G01N2333/5754 ,  G01N2333/5757 ,  G01N2333/58 ,  G01N2800/52

    摘要： The present invention relates to a method for the stratification of a subject having an acute or a chronic disease, wherein said disease effects endothelial function/dysfunction, comprising the steps of (i) taking a sample of bodily fluid from said subject; (ii) determining in said sample of bodily fluid the concentration of a vasoactive hormone or fragments thereof or precursors or fragments thereof having a length of at least 12 amino acid residues; (iii) stratifying said subjects into either of the categories: (a) responder to a medication for treatment of said disease, (b) non-responder to a medication for treatment of said disease not showing an unfavourable effect after having received said medication; (c) subjects showing an unfavourable effect after having received said medication. The invention also relates to the use of an antibody or a functional fragment thereof in the method according to the invention.

    摘要翻译： 本发明涉及一种用于分层具有急性或慢性疾病的受试者的方法，其中所述疾病影响内皮功能/功能障碍，包括以下步骤：（i）从所述受试者身上取体液样品; （ii）在所述体液样品中测定长度为至少12个氨基酸残基的血管活性激素或其片段或其前体或其片段的浓度; （iii）将所述受试者分为以下类别之一：（a）用于治疗所述疾病的药物的应答者，（b）在接受所述药物后不显示不利影响的所述疾病的治疗药物的无反应者; （c）在接受所述药物后显示不利影响的受试者。 本发明还涉及抗体或其功能片段在本发明方法中的用途。

    Method for the determination of the formation of endothelins for medical diagnostic purposes, and antibodies and kits for carrying out such a method
    77.
    发明授权
    Method for the determination of the formation of endothelins for medical diagnostic purposes, and antibodies and kits for carrying out such a method 有权
    用于确定用于医学诊断目的的内皮素形成的方法，以及用于进行这种方法的抗体和试剂盒

    公开(公告)号：US08124366B2

    公开(公告)日：2012-02-28

    申请号：US10588746

    申请日：2005-02-10

    申请人： Andreas Bergmann ,  Joachim Struck

    发明人： Andreas Bergmann ,  Joachim Struck

    IPC分类号： G01N33/00

    CPC分类号： C07K16/00 ,  G01N33/57488 ,  G01N33/6893 ,  G01N33/74 ,  G01N2333/5754 ,  Y10S435/975

    摘要： The invention relates to an in vitro method for detecting the formation of endothelins during serious illnesses, especially cardiovascular diseases, inflammations, sepsis and cancer, in whole blood, plasma or serum of a human patient for medical diagnosis. Using this method, relatively long-lasting peptide fragments, especially a C-terminal peptide fragment, of the processed primary prepro- or proendothelins that contain neither the actual biologically active endothelin nor its direct precursor, big endothelin, can be detected.

    摘要翻译： 本发明涉及用于检测人类患者的全血，血浆或血清中的严重疾病，特别是心血管疾病，炎症，败血症和癌症中内皮素的形成的体外方法。 使用该方法，可以检测到既不含有实际生物活性内皮素及其直接前体大内皮素的经加工的原代前原或内皮素的相对长效的肽片段，特别是C末端肽片段。