公开(公告)号：US11353400B2

公开(公告)日：2022-06-07

申请号：US16319157

申请日：2017-07-19

申请人： Altius Institute for Biomedical Sciences

发明人： John A. Stamatoyannopoulos ,  Shreeram Akilesh ,  Vivek Nandakumar

IPC分类号： G01N21/64 ,  C12Q1/6841 ,  C40B40/06 ,  G01N21/78 ,  G01N33/574 ,  G01N33/58 ,  G01N33/569 ,  A01N1/02 ,  C12N5/078 ,  C12N5/09 ,  C12P19/34 ,  G01N1/30 ,  C12Q1/6837 ,  C40B30/04 ,  G01N15/00

摘要： Disclosed herein are methods of detecting a target nucleic acid sequence, determining the localization of the target nucleic acid sequence, and/or quantifying the number of target nucleic acid sequences in a cell. This method may be used on small target nucleic acid sequences, and may be referred to as Nano-FISH.

公开(公告)号：US11345881B2

公开(公告)日：2022-05-31

申请号：US16063055

申请日：2016-12-08

申请人： Industry-Academic Cooperation Foundation of Ajou University

发明人： Jong-Young Kwak ,  Young-Hun Jeong ,  Jeong-Hwa Kim ,  Tae-Eon Kim

IPC分类号： C12M1/12 ,  C12M1/00 ,  C12M1/34 ,  C12N5/00 ,  C12N5/09

摘要： The present disclosure relates to a nanofiber structure for cell culture, a method for manufacturing the structure, and a cell analysis device including the nanofiber structure for cell culture. The structure includes a cell culture layer made of nanofibers; and a spacer protruding upward from a surface of the cell culture layer, wherein the spacer divides a region on the cell culture layer into at least two culturing regions, wherein the spacer is made of the same nanofibers as the cell culture layer and thus has a cell migration channel defined therein.

公开(公告)号：US11344627B2

公开(公告)日：2022-05-31

申请号：US16011992

申请日：2018-06-19

申请人： WISCONSIN ALUMNI RESEARCH FOUNDATION

发明人： Seungpyo Hong ,  Sin-Jung Park

IPC分类号： A61K47/69 ,  A61K49/18 ,  A61K47/64 ,  A61K31/519 ,  A61K49/00 ,  A61K47/59 ,  A61K47/62 ,  A61K9/50 ,  A61K9/51 ,  A61K38/00 ,  C07K14/705 ,  C12N5/0775 ,  C12N5/09 ,  B82Y5/00

摘要： Described herein are hybrid nanoparticles that are exosomes loaded with one or more nanoparticle dendrimers. Also included are pharmaceutical compositions including the hybrid nanoparticles and methods of making the hybrid nanoparticles. Also described is a method of treating a human subject by administering to the human subject the above-described hybrid nanoparticles.

公开(公告)号：US20220154165A1

公开(公告)日：2022-05-19

申请号：US17442282

申请日：2020-03-30

申请人： KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION

发明人： Masaru TANAKA ,  Fumihiro ARATSU

IPC分类号： C12N11/087 ,  C08F120/28 ,  C12N5/09 ,  C12N5/078

摘要： Particles for cell adhesion to be used for adhering highly adhesive cells present in an aqueous solution, said cell-adhesive particle comprising, in at least a part of the surface thereof, a wettable composition having an intermediate water content in the water-saturated state of 1-30 wt %.

公开(公告)号：US20220119762A1

公开(公告)日：2022-04-21

申请号：US17565972

申请日：2021-12-30

申请人： University of Florida Research Foundation, Inc.

发明人： Sarah V. ELLISON ,  Thomas Ettor ANGELINI ,  Scott Arthur BANKS ,  Duane MITCHELL ,  Cameron MORLEY ,  Catherine FLORES

IPC分类号： C12N5/00 ,  C12N5/09 ,  G01N1/30

摘要： Described herein are systems and methods relating to cellular micro-masonry. Systems and methods as described herein allow a user to create three-dimensional (3D) structures of cells disposed in a 3D culture medium. Systems and methods as described herein provide for the manipulation and construction of cellular structures on a single, cell-by-cell, basis.

公开(公告)号：US11280792B2

公开(公告)日：2022-03-22

申请号：US16123444

申请日：2018-09-06

申请人： ON-CHIP BIOTECHNOLOGIES CO., LTD. ,  SHIZUOKA PREFECTURE

发明人： Kazuo Takeda ,  Namiko Yamashita ,  Yuu Fujimura ,  Kaori Nishio ,  Yasuhiro Koh ,  Masaru Watanabe ,  Fumiaki Koizumi ,  Yuri Uehara

IPC分类号： G01N33/574 ,  C12N5/09 ,  G01N33/58

摘要： The object of the present invention is to provide an evaluation method capable of accurately determining a metastasis of cancer, the stage of cancer progression, or the malignancy of cancer.
The object can be solved by a method for detecting the degree of malignancy of each of the circulating tumor cells, characterized by the following steps: (a) bringing an epithelial cell-binding component, which specifically binds to a marker molecule expressed on epithelial cells and is fluorescently-labeled or luminescent enzyme-labeled, and a mesenchymal cell-binding component, which specifically binds to a marker molecule expressed on mesenchymal cells and is fluorescently-labeled or luminescent enzyme-labeled, into contact with a sample that possibly contains circulating tumor cells, (b) detecting a fluorescence signal or a luminescence signal of the epithelial cell-binding component and a fluorescence signal or a luminescence signal of the mesenchymal cell-binding component of each of the cells, and (c) determining the degree of epithelial-mesenchymal transition of circulating tumor cells based on the signal amount of the epithelial cell-binding component (E) and the signal amount of the mesenchymal cell-binding component (M).

公开(公告)号：US11249073B2

公开(公告)日：2022-02-15

申请号：US14434200

申请日：2013-10-08

申请人： University of Washington through its Center for Commercialization

发明人： Daniel T. Chiu ,  Wei Sun ,  Jiangbo Yu ,  Changfeng Wu

IPC分类号： G01N33/52 ,  C12N5/09 ,  C09K11/06 ,  C12N13/00

摘要： The present disclosure provides metal-containing (MC) semiconducting (SC) Pdots (MC-SC-Pdots) with beneficial functionalities in both cellular imaging and manipulation, among other applications. The Pdots comprise at least one nanoparticle comprising at least one metal, and a semiconducting polymer associated with the nanoparticle.

公开(公告)号：US20210371825A1

公开(公告)日：2021-12-02

申请号：US17285908

申请日：2019-10-16

申请人： BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM

发明人： Raghu KALLURI ,  Valerie LEBLEU ,  Yang CHEN

IPC分类号： C12N5/071 ,  C12N5/09 ,  G01N33/50

摘要： Provided herein are methods of enhancing the production of organoids from primary tissues, which can be either normal or cancerous, by culturing the primary tissues in the presence of collagen I homotrimers. To this end, also provided are cell culture media comprising exogenous collagen I homotrimers. Also provided are organoids produced by the disclosed methods as well as methods of using the organoids so produced.

公开(公告)号：US20210363493A1

公开(公告)日：2021-11-25

申请号：US16468100

申请日：2018-10-17

申请人： BOE TECHNOLOGY GROUP CO., LTD.

发明人： Defeng MAO

IPC分类号： C12N5/09 ,  B01L3/00 ,  G01N33/569

摘要： Embodiments of the present disclosure provide a target capturing apparatus and a manufacturing method thereof, and a target detecting method. The target capturing apparatus includes a cavity structure, the cavity structure includes: an inlet portion, an outlet portion and a capture region positioned between the inlet portion and the outlet portion, and the capture region includes a capture component, and a combination specifically combined with a to-be-captured target is included in the capture component so as to capture the target in a sample entering the cavity structure.

公开(公告)号：US11180735B2

公开(公告)日：2021-11-23

申请号：US15793249

申请日：2017-10-25

申请人： The Board of Trustees of the Leland Stanford Junior University

发明人： James Neal ,  Calvin Jay Kuo

IPC分类号： C12N5/09 ,  A61K39/395 ,  C12N5/0783 ,  G01N33/574 ,  C07K16/28 ,  G01N33/50 ,  A61K39/00

摘要： Functional in vitro assays are provided for determining patient specific responsiveness to immunotherapy agents within a clinically actionable time frame.