公开(公告)号：US06451189B2

公开(公告)日：2002-09-17

申请号：US09809143

申请日：2001-03-16

Applicant: N. Leigh Anderson ,  Norman G. Anderson ,  Jack Goodman

Inventor： N. Leigh Anderson ,  Norman G. Anderson ,  Jack Goodman

IPC: B01D5702

CPC classification number: G01N27/44795 ,  G01N27/44773 ,  G01N27/44782 ,  G01N2001/288 ,  Y10T436/113332 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/255

Abstract: The present invention provides an integrated, fully automated, high-throughput system for two-dimensional electrophoresis comprised of gel-making machines, gel processing machines, gel compositions and geometries, gel handling systems, sample preparation systems, software and methods. The system is capable of continuous operation at high-throughput to allow construction of large quantitative data sets.

Abstract translation: 本发明提供了一种用于由凝胶制造机，凝胶加工机，凝胶组合物和几何形状，凝胶处理系统，样品制备系统，软件和方法组成的二维电泳的集成的全自动化高通量系统。 该系统能够以高通量连续运行，从而构建大量的数据集。

公开(公告)号：US20020104759A1

公开(公告)日：2002-08-08

申请号：US10013404

申请日：2001-12-07

Applicant: The Board of Governors of the University of Alberta and Micralyne Inc.

Inventor： Christopher J. Backhouse

IPC: G01N027/26 ,  G01N027/447

CPC classification number: B01L3/5027 ,  B01L3/502753 ,  B01L3/502784 ,  B01L7/52 ,  B01L2200/0605 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2400/0415 ,  B01L2400/0421 ,  G01N27/44773 ,  G01N27/44782 ,  G01N27/44791

Abstract: A microanalysis system, comprising a microchip, a common well in the microchip, and multiple wells in the microchip, each of the multiple wells being connected for fluid flow to the common well by a channel. The common well is a waste well, and the multiple wells are sample introduction wells. The common well is at the center of the microchip. The channels radiate outward from the common well, and are equally circumferentially spaced. A buffer introduction channel is provided that intersects each of the channels.

Abstract translation: 一种微量分析系统，包括微芯片，微芯片中的普通井和微芯片中的多个孔，多个井中的每一个连接用于通过通道流体流入公共井。 普通井是废井，多井是采样井。 普通井是微芯片的中心。 通道从公共井向外辐射，并且周向间隔开。 提供了与每个通道相交的缓冲器引入通道。

公开(公告)号：US06406604B1

公开(公告)日：2002-06-18

申请号：US09436186

申请日：1999-11-08

Applicant: Norberto A. Guzman

Inventor： Norberto A. Guzman

IPC: G02N2726

CPC classification number: G01N27/44704 ,  G01N27/44726 ,  G01N27/44743 ,  G01N27/44773

Abstract: An electrophoresis apparatus is generally disclosed for sequentially analyzing a single sample or multiple samples having one or more analytes in high or low concentrations. The apparatus comprises a relatively large-bore transport capillary which intersects with a plurality of small-bore separation capillaries. Analyte concentrators, having antibody-specific (or related affinity) chemistries, are stationed at the respective inter-sections of the transport capillary and separation capillaries to bind one or more analytes of interest. The apparatus allows the performance of two or more dimensions for the optimal separation of analytes.

Abstract translation: 通常公开了电泳装置，用于顺序分析具有高或低浓度的一种或多种分析物的单个样品或多个样品。 该装置包括与多个小孔分离毛细管相交的相对大的输送毛细管。 具有抗体特异性（或相关亲和力）化学物质的分析物浓缩器驻留在运输毛细管和分离毛细管的相应切片处，以结合一种或多种感兴趣的分析物。 该装置允许执行两个或多个维以分析物的最佳分离。

公开(公告)号：US20020070166A1

公开(公告)日：2002-06-13

申请号：US10013783

申请日：2001-12-07

Applicant: Board of Governors of the University of Alberta

Inventor： Christopher J. Backhouse

IPC: B01D011/00

CPC classification number: B01L3/502784 ,  B01L3/502753 ,  B01L7/52 ,  B01L2200/141 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2400/0415 ,  G01N1/34 ,  G01N27/44773 ,  G01N27/44782 ,  G01N27/44791

Abstract: A method of sample separation begins with the step of moving product and contaminant along a first channel in a first direction at different speeds for the product and contaminant until contaminant separates from the product and is lost by dilution in a waste well. Then the direction of movement of the product and contaminant is reversed to separate product from the contaminant. After product is separated from contaminant it may be detected using conventional detectors. To ensure effective dilution of contaminant, it is preferred that there be a delay or pause between forward movement of the sample and reverse movement.

Abstract translation: 样品分离的方法开始于以不同速度沿着第一方向沿着第一通道移动产物和污染物的步骤，用于产物和污染物，直到污染物与产物分离，并通过稀释在废物井中而丢失。 然后将产品和污染物的运动方向相反，以将产品与污染物分开。 产品与污染物分离后，可以使用常规检测器进行检测。 为了确保污染物的有效稀释，优选在样品的向前运动和反向运动之间存在延迟或暂停。

公开(公告)号：US20020070113A1

公开(公告)日：2002-06-13

申请号：US09738462

申请日：2000-12-13

Applicant: The Regents of the University of California

Inventor： Robin R. Miles ,  Amy Wei-Yun Wang ,  Raymond P. Mariella JR.

IPC: G01N027/26 ,  G01N027/447

CPC classification number: G01N27/44773

Abstract: A fluidic channel patterned with a series of thin-film electrodes makes it possible to move and concentrate DNA in a fluid passing through the fluidic channel. The DNA has an inherent negative charge and by applying a voltage between adjacent electrodes the DNA is caused to move. By using a series of electrodes, when one electrode voltage or charge is made negative with respect to adjacent electrodes, the DNA is repelled away from this electrode and attached to a positive charged electrode of the series. By sequentially making the next electrode of the series negative, the DNA can be moved to and concentrated over the remaining positive electrodes.

Abstract translation: 用一系列薄膜电极图案化的流体通道使得可以将DNA移动并浓缩在通过流体通道的流体中。 DNA具有固有的负电荷，并且通过在相邻电极之间施加电压使DNA发生移动。 通过使用一系列电极，当相对于相邻电极使一个电极电压或电荷为负极时，DNA将从该电极排出并附着到该串联的正带电电极。 通过顺序地使串联的下一个电极为阴性，DNA可以移动到其余正极上并集中在剩余的正电极上。

公开(公告)号：US20020056640A1

公开(公告)日：2002-05-16

申请号：US10016483

申请日：2001-12-10

Inventor： David S. Soane ,  Zoya M. Soane

IPC: G01N027/26 ,  G01N027/447

CPC classification number: B01D57/02 ,  B01F13/0059 ,  B01F13/0076 ,  B01J19/0093 ,  B01J2219/00853 ,  B01J2219/00912 ,  B82Y10/00 ,  B82Y40/00 ,  C07K1/26 ,  G01N27/44752 ,  G01N27/44773 ,  G03F7/0002

Abstract: Devices and methods are disclosed for moving charged molecules through a medium by the application of a plurality of electrical fields of sufficient strength and applied for sufficient amounts of time so as to move the charged molecules through the medium. The devices although preferably small in size, preferably generate large numbers (100 or more) of electrical fields to a movement area which preferably contains a liquid buffered or gel medium. Mixtures of charged molecules are pulled through the gel by the force of the electrical fields. The fields are preferably activated simultaneously or sequentially one after another at various speeds to create complex force field distributions or moving field waves along the separation medium. Charged molecules capable of moving quickly through the gel will be moved along by the faster moving field waves and be separated from slower moving molecules. The fields can be activated by computer software and can be used to move molecules away from and toward each other to obtain rapid and complex chemical synthesis, sequencing or reaction protocols.

Abstract translation: 公开的装置和方法用于通过施加足够强度的多个电场来移动带电分子通过介质，并施加足够的时间以便使带电分子通过介质移动。 尽管尺寸优选小的装置优选地对于优选含有液体缓冲或凝胶介质的运动区域产生大量（100个或更多个）电场。 带电分子的混合物被电场的力拉入凝胶。 这些场优选地以各种速度一个接一个地同时或顺序地被激活，以产生沿着分离介质的复数的力场分布或移动的场波。 能够快速移动通过凝胶的带电分子将沿着更快的移动场波移动并与较慢的移动分子分离。 这些领域可以通过计算机软件激活，并且可以用于将分子移开和相互移动，以获得快速和复杂的化学合成，测序或反应方案。

公开(公告)号：US20020008029A1

公开(公告)日：2002-01-24

申请号：US09780638

申请日：2001-02-10

Inventor： Stephen J. Williams ,  Hong Dong Tan ,  Hung Pin Kao ,  Wyatt N. Vreeland

IPC: G01N027/26 ,  G01N027/447

CPC classification number: C07K1/28 ,  B01L3/5027 ,  B01L3/502776 ,  B01L3/502784 ,  B01L2200/0605 ,  B01L2300/0816 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/049 ,  G01N27/44743 ,  G01N27/44747 ,  G01N27/44773 ,  G01N27/44791

Abstract: A method and device for injecting a liquid sample into an electrolyte channel in a microfluidics device is disclosed. The device has a channel network that includes an electrolyte channel having upstream and downstream channel portions and first, second, and third side channels that intersect the electrolyte channel between the two channel portions at first, second, and third ports, respectively. In the method, a sample is moved electrokinetically into the electrolyte channel, to form a defined sample volume therein. By simultaneously controlling the voltage applied to the three side channels, and at least one of the upstream and downstream channel end portions, the sample volume element can be shaped to have a desired leading- and trailing-edge shape and/or distribution of sample components within the volume elements.

Abstract translation: 公开了一种用于将液体样品注入微流体装置中的电解质通道的方法和装置。 该装置具有通道网络，其包括具有上游和下游通道部分的电解质通道以及分别在第一，第二和第三端口处的两个通道部分之间与电解质通道相交的第一，第二和第三侧通道。 在该方法中，将样品电动移动到电解质通道中，以在其中形成限定的样品体积。 通过同时控制施加到三个侧通道的电压以及上游和下游通道端部中的至少一个，样品体积元件可以被成形为具有期望的前沿和后沿形状和/或样品组分的分布 在音量元素内。

公开(公告)号：US20010023825A1

公开(公告)日：2001-09-27

申请号：US09725300

申请日：2000-11-29

Inventor： Leonid Frumin ,  Sergey E. Peltek ,  Gleb V. Zilberstein ,  Shmuel Bukshpan ,  Uriel Halavee

IPC: G01N027/26 ,  G01N027/447

CPC classification number: G01N27/44795 ,  G01N27/44773 ,  G01N2030/0035

Abstract: A method is disclosed for moving, isolating and/or identifying particles in a sample by placing said sample in a spatially varying electrical field wherein the spatially varying electrical field is following a mathematical nonmonotonous function, selected from the group consisting of linear, hyperbolic, parabolic, parabolic functions or ynullxp/q and combinations thereof wherein p q means an integer. Also various devices are disclosed for performing the method.

Abstract translation: 公开了通过将样品置于空间变化的电场中来移动，分离和/或识别样品中的颗粒的方法，其中空间变化的电场遵循数学非单调函数，其选自线性，双曲线，抛物线 抛物线函数或y〜xp / q及其组合，其中pq表示整数。 还公开了用于执行该方法的各种装置。

公开(公告)号：US06277258B1

公开(公告)日：2001-08-21

申请号：US09306645

申请日：1999-05-06

Applicant: Cornelius F. Ivory ,  Zheng Huang ,  Fred J. Schuetze

Inventor： Cornelius F. Ivory ,  Zheng Huang ,  Fred J. Schuetze

IPC: G01N2726

CPC classification number: G01N27/44795 ,  G01N27/44743 ,  G01N27/44773

Abstract: An electrophoretic device and method for focusing a charged solute is disclosed. The device includes a first chamber for receiving a fluid medium, the first chamber having an inlet for introducing a first liquid to the chamber and an outlet for exiting the first liquid from the chamber; a second chamber comprising an electrode array, the second chamber having an inlet for introducing a second liquid to the chamber and an outlet for exiting the second liquid from the chamber; and a porous material separating the first and second chambers. The device's electrode array includes a plurality of electrodes and generates an electric field gradient profile which can be dynamically controlled. In the method, a charged solute is introduced into a fluid medium followed by the application of a hydrodynamic force. Opposing the hydrodynamic force with an electric field gradient results in solute focusing in the fluid medium. The electric field gradient is generated by an electrode array by individually adjusting the electrode voltages.

Abstract translation: 公开了一种用于聚焦带电溶质的电泳装置和方法。 所述装置包括用于接收流体介质的第一室，所述第一室具有用于将第一液体引入所述室的入口和用于从所述室离开所述第一液体的出口; 包括电极阵列的第二腔室，所述第二腔室具有用于将第二液体引入所述腔室的入口和用于从所述腔室排出所述第二液体的出口; 以及分离第一和第二室的多孔材料。 器件的电极阵列包括多个电极，并产生可以动态控制的电场梯度分布。 在该方法中，将带电溶质引入流体介质中，随后施加流体动力。 用电场梯度对流动力产生流体介质中的溶质聚焦。 通过单独调节电极电压由电极阵列产生电场梯度。

公开(公告)号：US06176990B1

公开(公告)日：2001-01-23

申请号：US08973933

申请日：1997-12-16

Applicant: Thomas D. Yager ,  Paul Waterhouse ,  Alexandre M. Izmailov ,  Bruno C. Maruzzo ,  John K. Stevens ,  Marina T. Larson

Inventor： Thomas D. Yager ,  Paul Waterhouse ,  Alexandre M. Izmailov ,  Bruno C. Maruzzo ,  John K. Stevens ,  Marina T. Larson

IPC: G01N27453

CPC classification number: G01N27/44773 ,  G01N27/44704 ,  G01N27/44791

Abstract: A microelectrophoresis chip comprises a substrate in which there are formed one or more channels, one channel for each sample to be evaluated. The channels extend for the length of the chip, a distance of generally around 1 cm, and are about 1 to 10 &mgr;m wide and 1 to 10 &mgr;m in depth. The channels are filled with a homogeneous separation matrix which acts as an obstacle to the electrophoretic migration of the charged molecules. Microelectrodes disposed in the channels are used to induce an electric field within the homogeneous separation medium. When a voltage is applied across two or more of the microelectrodes, the charged molecules are induced to move and separate according to the electric field density, the type of solvent film, and the charge, shape and size of the charged molecule. The chip may further comprise detectors, such as light polarization detectors, fluorescence emission detectors, biosensors, electrochemical sensors or other microcomponents which may include sites for enzymatic or chemical manipulation of the moved or separated charged molecules.

Abstract translation: 微电泳芯片包括其中形成一个或多个通道的基底，每个待评估样品的通道一个通道。 通道延长芯片的长度，通常约1厘米的距离，宽度约为1至10微米，深度为1至10毫米。 通道充满均匀的分离基质，其作为电荷分子的电泳迁移的障碍。 设置在通道中的微电极用于在均匀分离介质内诱发电场。 当电压施加到两个或更多个微电极上时，根据电场密度，溶剂膜的类型和带电分子的电荷，形状和尺寸，诱导带电分子移动和分离。 芯片还可以包括诸如光偏振检测器，荧光发射检测器，生物传感器，电化学传感器或其它微元件的检测器，其可以包括用于酶或分离带电分子的酶或化学操作的位点。