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公开(公告)号：US20210381032A1
公开(公告)日：2021-12-09
申请号：US17289279
申请日：2019-10-31
发明人： Luke T. Daum , Gerald W. Fischer
IPC分类号： C12Q1/689 , C12Q1/6816 , C12Q1/6851 , C12Q1/70
摘要： Disclosed is an enhanced method for rapid and cost-effective analysis of sequences of a microorganism by qPCR. These methods identify allelic variation, SNPs, and genetic mutations of a particular gene such as those responsible for conferring resistance or sensitivity to an antibiotic, chemotherapy, or another chemical compound. By selection of appropriate gene regions, mutation loci that confer resistance to key antibiotics can be identified by qPCR. Additionally, the approach can identify heteroresistant strains, e.g., populations of strains from a sample that contain both mutation and wild-type nucleotides. By selecting appropriate that bind efficiently to the area of mutation can identify resistance conferring mutations. Methods are useful to sequences derived from viral agents, such as influenza virus, bacterial agents, such as tuberculosis bacteria, and cancer cells.
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公开(公告)号：US20210079450A1
公开(公告)日：2021-03-18
申请号：US17010410
申请日：2020-09-02
发明人： Luke T. Daum , Gerald W. Fischer
IPC分类号： C12Q1/686 , C12Q1/6869
摘要： The invention is directed to compositions and methods for collecting, transporting, and storing, without refrigeration, biological materials, which may comprise samples of biological, clinical, forensic, and/or environmental origin. These compositions preserve the viability of the collected organisms and/or the RNA/DNA and proteins in the sample composition mixture and permit the long-term storage of samples. Compositions are compatible with subsequent manipulation of the sample, including propagation and culture of the collected microorganisms, or isolation, purification, detection, and characterization of proteins, nucleic acids, and other macromolecules. When the compositions containing microorganisms and any polynucleotides therein are further processed, such as by nucleic acid testing, there is an increased ability to detect, isolate, purify and/or characterize select microbes and their components, such as nucleic acids, when compared to conventional microbial transport media that contain interfering substances and RNA/DNA extraction is not required prior to PCR analysis. In particular, the compositions disclosed allow for the collection, transport and storage of biological samples for extended periods at ambient temperature, while maintaining the integrity of the macromolecules of the sample for subsequent extraction, identification, and quantitation.
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公开(公告)号：US20190352378A1
公开(公告)日：2019-11-21
申请号：US16530520
申请日：2019-08-02
摘要： The invention is directed to compositions and methods for stimulating, enhancing or modulating the immune system of a patient before or after infection by a pathogen, and in particular multidrug resistant (MDR) MTB and extremely drug resistant (XDR) MTB. Compositions of the invention contain non-naturally occurring antigens that generate an effective cellular and/or humoral immune response to MTB and/or antibodies that are specifically reactive to MTB antigens. The greater activity of the immune system generated by a vaccine of the invention increases generation of memory T cells that provide for a greater and/or extended response to an MTB infection. Responses involve an increased generation of antibodies that enhance immunity against MTB infection and promote an enhanced phagocytic response. Monoclonal antibodies produced by the non-naturally occurring antigens enhance phagocytosis and killing of mycobacteria by phagocytic cells, enhance clearance of MTB from the blood and modulate immunity and cytokine responses.
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公开(公告)号：US20180258419A1
公开(公告)日：2018-09-13
申请号：US15972341
申请日：2018-05-07
发明人： Gerald W. Fischer , Luke T. Daum
摘要： The invention is directed to compositions and methods for rapidly and efficiently extracting nucleic acids and/or targeted nucleic acids sequences from biological samples. The methods of the invention comprise combining the sample with a buffer and magnetic silicon beads and concentrating the beads with a magnet or other electrical field. Liquid may be removed, or not, and an alkaline buffer is added followed by magnetic carboxy beads in a binding buffer so that nucleic acids transfer to the carboxy beads, which can be easily and quickly isolated once again with a magnet. Total nucleic acid extraction is greatly enhanced. Extracted nucleic acids can be analyzed, for example, by PCR wherein the nucleic acids can be identified and characterized. Carboxy beads may also contain a ligand so as to target specific nucleic acid sequences. The invention is also directed to kits comprising the tools and compositions for performing the methods of the invention.
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公开(公告)号：US09976136B2
公开(公告)日：2018-05-22
申请号：US15152871
申请日：2016-05-12
发明人： Gerald W. Fischer , Luke T. Daum
CPC分类号： C12N15/1013 , C12Q1/686
摘要： The invention is directed to compositions and methods for rapidly and efficiently extracting nucleic acids and/or targeted nucleic acids sequences from biological samples. The methods of the invention comprise combining the sample with a buffer and magnetic silicon beads and concentrating the beads with a magnet or other electrical field. Liquid may be removed, or not, and an alkaline buffer is added followed by magnetic carboxy beads in a binding buffer so that nucleic acids transfer to the carboxy beads, which can be easily and quickly isolated once again with a magnet. Total nucleic acid extraction is greatly enhanced. Extracted nucleic acids can be analyzed, for example, by PCR wherein the nucleic acids can be identified and characterized. Carboxy beads may also contain a ligand so as to target specific nucleic acid sequences. The invention is also directed to kits comprising the tools and compositions for performing the methods of the invention.
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公开(公告)号：US09598462B2
公开(公告)日：2017-03-21
申请号：US13750771
申请日：2013-01-25
发明人： Gerald W. Fischer , Luke T. Daum
CPC分类号： C07K7/06 , A61K39/12 , A61K2039/6043 , A61K2039/6068 , A61K2039/70 , C07K7/08 , C07K14/005 , C07K14/11 , C07K14/35 , C07K2319/40 , C07K2319/42 , C12N2760/16122 , C12N2760/16134
摘要： The invention relates to composite antigens comprising a peptide with contiguous amino acid sequence derived from a plurality of antigenic epitopes of one or more pathogens that induces an immune response in a mammal that is protective against infection by the one or more pathogens. In addition, the invention relates to vaccines comprising composite antigens and to method for treating and preventing an infection.
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67.发明申请公开(公告)号：US20160333339A1
公开(公告)日：2016-11-17
申请号：US15152871
申请日：2016-05-12
发明人： Gerald W. Fischer , Luke T. Daum
CPC分类号： C12N15/1013 , C12Q1/686
摘要： The invention is directed to compositions and methods for rapidly and efficiently extracting nucleic acids and/or targeted nucleic acids sequences from biological samples. The methods of the invention comprise combining the sample with a buffer and magnetic silicon beads and concentrating the beads with a magnet or other electrical field. Liquid may be removed, or not, and an alkaline buffer is added followed by magnetic carboxy beads in a binding buffer so that nucleic acids transfer to the carboxy beads, which can be easily and quickly isolated once again with a magnet. Total nucleic acid extraction is greatly enhanced. Extracted nucleic acids can be analyzed, for example, by PCR wherein the nucleic acids can be identified and characterized. Carboxy beads may also contain a ligand so as to target specific nucleic acid sequences. The invention is also directed to kits comprising the tools and compositions for performing the methods of the invention.
摘要翻译： 本发明涉及用于从生物样品快速和有效地提取核酸和/或靶向核酸序列的组合物和方法。 本发明的方法包括将样品与缓冲液和磁性硅珠组合，并用磁体或其他电场浓缩珠粒。 液体可以被去除或不被除去，并且在结合缓冲液中加入碱性缓冲剂，随后加入磁性羧基珠，使得核酸转移到羧基珠上，再次用磁体容易地和快速地分离出来。 总核酸提取量大大提高。 可以例如通过PCR来分析提取的核酸，其中核酸可以被鉴定和表征。 羧基珠还可以含有配体，以靶向特定的核酸序列。 本发明还涉及包括用于执行本发明方法的工具和组合物的试剂盒。
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公开(公告)号：US09365904B2
公开(公告)日：2016-06-14
申请号：US13890512
申请日：2013-05-09
发明人： Luke T. Daum , Gerald W. Fischer
CPC分类号： C12Q1/701 , C12Q1/6869 , C12Q1/689 , C12Q2600/156 , C12Q2563/113 , C12Q2563/116
摘要： Disclosed is an enhanced method for rapid and cost-effective analysis of sequences of a microorganism by semi-conductor sequencing, preferably ion-torrent sequencing. This method provides for full length analysis and of multiple areas (e.g. genes) of multiple genomes. These methods identify genetic mutations of a particular gene that are responsible for conferring resistance or sensitivity to an antibiotic or other chemical compound. Multiple different species, strains and/or serotypes of a particular organism are rapidly and efficiently screened and mutations identified along with the complete genome of an organism. By selecting primers pairs of similar size and GC content that produce amplicons with sequences spanning the entire genome, a single PCR reaction analyzed by ion torrent methodology can determine the sequence of a complete genome. Methods are useful to sequences the genomes of viral agents, such as influenza virus, and bacterial agents, such as tuberculosis bacteria.
摘要翻译： 公开了一种用于通过半导体测序（优选离子束测序）快速和成本有效地分析微生物序列的增强方法。 该方法提供了全长分析和多个基因组的多个区域（例如基因）。 这些方法鉴定了负责赋予抗生素或其他化合物的抗性或敏感性的特定基因的遗传突变。 特定生物体的多种不同物种，菌株和/或血清型被快速有效地筛选，突变与生物体的完整基因组一起鉴定。 通过选择产生具有跨越整个基因组的序列的扩增子的相似大小和GC含量的引物对，通过离子洪流方法分析的单个PCR反应可以确定完整基因组的序列。 方法可用于对病毒试剂的基因组进行排序，例如流感病毒和细菌试剂如结核菌。
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公开(公告)号：US20150182606A1
公开(公告)日：2015-07-02
申请号：US14584110
申请日：2014-12-29
CPC分类号： A61K38/4886 , A61K9/0073 , A61K9/16 , A61K9/50 , A61K45/06 , A61L29/16 , A61L31/16 , A61L2300/254 , A61L2300/404 , A61L2300/45 , A61P31/00 , A61P31/06 , C12N9/6489 , C12Y304/24075 , Y02A50/473
摘要： The present invention is directed to compositions and methods for preventing and/or treating diseases and disorders of patients caused by non-Staphylococcal microorganisms. In particular, compositions and methods contain lysostaphin, altered forms of lysostaphin as compared to wild-type, and synergistic combinations of lysostaphin plus additional conventional treatments such as other enzyme, antibiotic and/or antibody treatment. The invention is also directed to detecting and identifying altered forms of lysostaphin that possess increased efficacy against infections as compared to wild-type lysostaphin, and forms that generate a minimal or no immune response in a patient. The invention is also directed to method of manufacturing lysostaphin and altered forms of lysostaphin, and compositions that direct the lysostaphin to the site of the infection such as aerosolized nanoparticles.
摘要翻译： 本发明涉及用于预防和/或治疗由非葡萄球菌微生物引起的患者疾病和病症的组合物和方法。 特别地，组合物和方法含有溶葡萄球菌素，与野生型相比的溶葡萄球菌素的改变形式，以及溶葡萄球菌素加其他常规治疗如其它酶，抗生素和/或抗体治疗的协同组合。 本发明还涉及检测和鉴定与野生型溶葡萄球菌素相比具有增强的感染效力的溶葡萄球菌的改变形式，以及在患者中产生最小或无免疫应答的形式。 本发明还涉及制备溶葡萄球菌素和改变形式的溶葡萄球菌素的方法，以及将溶葡萄球菌引导至感染部位如烟雾化纳米粒子的组合物。
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公开(公告)号：US20150064198A1
公开(公告)日：2015-03-05
申请号：US14473322
申请日：2014-08-29
发明人： Gerald W. Fischer , Luke T. Daum , Clara Jebet Sei
CPC分类号： A61K39/04 , A61K39/12 , A61K39/40 , A61K2039/521 , A61K2039/55505 , A61K2039/55566 , A61K2039/575 , A61K2039/6037 , A61K2039/6081 , A61K2039/70 , C07K14/35 , C07K16/1289 , C07K2317/73 , C07K2319/00 , C07K2319/31 , C12N2760/16134 , G01N33/5055
摘要： The invention is directed to compositions and methods for generating or enhancing the immune system of a patient against infection by a pathogen, and in particular MTB. Compositions of the invention contain one or more non-naturally occurring antigens that generate an effective cellular or humoral immune response to MTB and/or antibodies that are specifically reactive to mycolic acid or to the surface of MTB. The greater activity of the immune system generated by a vaccine of the invention involve an conjugation of peptides to increase in the generation of memory T cells that provide for a greater and/or longer lived or extended response to an MTB infection. Preferably a response involves an increased generation of antibodies that enhance immunity against MTB infection and promote an enhanced phagocytic response.
摘要翻译： 本发明涉及用于产生或增强患者免受病原体感染，特别是MTB感染的免疫系统的组合物和方法。 本发明的组合物含有一种或多种非天然存在的抗原，其产生对MTB和/或抗体的有效细胞或体液免疫应答，所述MTB和/或抗体对霉酚酸或对MTB的表面具有特异性的反应性。 由本发明的疫苗产生的免疫系统的更大活性涉及肽的缀合，以增加记忆性T细胞的产生，其提供对MTB感染的更大和/或更长寿命或延长的应答。 优选地，响应涉及提高针对MTB感染的免疫力的抗体的增加的产生并且促进增强的吞噬反应。
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