公开(公告)号：US20110269159A1

公开(公告)日：2011-11-03

申请号：US12771634

申请日：2010-04-30

申请人： John Lewis Emerson Campbell ,  Graham Davis ,  John Emegbero Omakor ,  Adam Roger Moss ,  Ganesh Guhados

发明人： John Lewis Emerson Campbell ,  Graham Davis ,  John Emegbero Omakor ,  Adam Roger Moss ,  Ganesh Guhados

IPC分类号： G01N33/53

CPC分类号： G01N33/54393 ,  G01N33/5306 ,  G01N33/5375 ,  G01N33/74 ,  G01N2333/475 ,  Y10T436/25125 ,  Y10T436/25375

摘要： Methods and devices for reducing interference from leukocytes in an analyte immunoassay are provided. In one embodiment, a method is provided comprising the steps of amending a biological sample such as a whole blood sample with one or more leukocidal reagents that reduce or eliminate the metabolic activity of leukocytes, and performing an immunoassay on the amended sample to determine the concentration of analyte in the sample. Preferably, the sample is amended with one or more enzymes and optionally one or more enzyme substrates and cofactors.

摘要翻译： 提供了用于减少分析物免疫测定中白细胞干扰的方法和装置。 在一个实施方案中，提供了一种方法，其包括以下步骤：将生物样品例如全血样品与一种或多种减少或消除白细胞的代谢活性的白细胞介素试剂进行修饰，并对经修订的样品进行免疫测定以确定浓度 的样品中的分析物。 优选地，用一种或多种酶和任选的一种或多种酶底物和辅因子修饰样品。

公开(公告)号：US20110256573A1

公开(公告)日：2011-10-20

申请号：US13051705

申请日：2011-03-18

申请人： Graham Davis ,  Stephen C. Wardlaw ,  Robert A. Levine ,  Darryn W. Unfricht ,  Niten Lalpuria ,  Paul G. Mitsis

发明人： Graham Davis ,  Stephen C. Wardlaw ,  Robert A. Levine ,  Darryn W. Unfricht ,  Niten Lalpuria ,  Paul G. Mitsis

IPC分类号： C12Q1/02 ,  C12M1/34

CPC分类号： G01N33/726 ,  G01N15/1475 ,  G01N2015/0073

摘要： A method and apparatus for determining at least one hemoglobin related parameter of a whole blood sample is provided. The method includes the steps of: a) depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by an interior surface of a first panel, and an interior surface of a second panel, and the chamber has a height extending between the interior surfaces of the panels, wherein the chamber is configured to increase the oxygenation state of the sample to a substantially oxygenated state within a predetermined amount of time after entry into the chamber; b) imaging the at least one red blood cell contacting the interior surfaces, and producing image signals; c) determining an optical density of at least a portion of the imaged red blood cell contacting both interior surfaces; and d) determining the at least one hemoglobin related parameter of the red blood cell contacting the interior surfaces, using the determined optical density and a molar extinction coefficient for oxygenated hemoglobin.

摘要翻译： 提供了一种用于确定全血样品的至少一种血红蛋白相关参数的方法和装置。 该方法包括以下步骤：a）将样品沉积到适于静态保持样品进行分析的分析室中，所述室由第一面板的内表面和第二面板的内表面限定，并且所述室具有 在所述面板的内表面之间延伸的高度，其中所述腔被配置为在进入所述腔室之后的预定量的时间内将所述样品的氧合状态增加到基本上氧合状态; b）对与内表面接触的至少一个红细胞进行成像，并产生图像信号; c）确定接触两个内表面的成像红细胞的至少一部分的光密度; 以及d）使用确定的光密度和氧合血红蛋白的摩尔消光系数来确定与内表面接触的红细胞的至少一种血红蛋白相关参数。

公开(公告)号：US20110243794A1

公开(公告)日：2011-10-06

申请号：US13077189

申请日：2011-03-31

申请人： Stephen C. Wardlaw

发明人： Stephen C. Wardlaw

IPC分类号： G01N33/50

CPC分类号： B01L3/502715 ,  B01L9/52 ,  B01L2300/0654 ,  B01L2300/0822 ,  B01L2300/087 ,  B01L2300/0877

摘要： A cartridge for analyzing a biologic fluid sample is provided that includes a base plate, a sample inlet port, a first chamber wall, a second chamber wall, and an optically transparent cover panel disposed in contact with the first and second chamber walls. The base plate has a body with a chamber surface, a body passage, and a chamber entry passage. The body passage is in fluid communication with the chamber entry passage, and the chamber entry passage extends through to the chamber surface. The sample inlet port has an inlet passage in fluid communication with the body passage. The first and second chamber walls each have a height extending outwardly from the chamber surface, and the two walls are spaced apart from one another. The cover panel is sufficiently flexible to deflect and contact a central region of the chamber surface.

摘要翻译： 提供了用于分析生物流体样品的盒，其包括底板，样品入口端口，第一室壁，第二室壁和布置成与第一和第二室壁接触的光学透明盖板。 基板具有具有室表面的主体，主体通道和室进入通道。 身体通道与腔室进入通道流体连通，并且腔室进入通道延伸到腔室表面。 样品入口具有与身体通道流体连通的入口通道。 第一和第二室壁各自具有从室表面向外延伸的高度，并且两个壁彼此间隔开。 盖板足够柔性以偏转和接触室表面的中心区域。

公开(公告)号：US20110230740A1

公开(公告)日：2011-09-22

申请号：US13116837

申请日：2011-05-26

申请人： Robert A. Levine ,  Stephen C. Wardlaw ,  Darryn W. Unfricht ,  Niten V. Lalpuria

发明人： Robert A. Levine ,  Stephen C. Wardlaw ,  Darryn W. Unfricht ,  Niten V. Lalpuria

IPC分类号： A61B5/1455

CPC分类号： G01N33/80 ,  A61B5/145 ,  G01N15/1463 ,  G01N15/1475 ,  G01N33/48 ,  G01N33/49 ,  G01N33/721 ,  G01N33/86

摘要： A method for determining the hematocrit of a blood sample is provided that includes the steps of: 1) depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by the interior surfaces of first and second panels and a height extending there between, wherein both panels are transparent, and the height is such that at least some of the red blood cells within the sample contact both interior surfaces of the panels and one or more lacunae within the quiescent sample extend between the interior surfaces; 2) imaging at least a portion of the quiescent sample, which sample portion contains the red blood cells and one or more lacunae to determine an optical density of the imaged portion of the sample on a per image unit basis; 3) selecting and averaging the optical density values of the image units aligned with the red blood cells contacting the interior surfaces, and assigning an upper boundary value of 100% to the average optical density value of those image units; 4) selecting the optical density values of the image units aligned with the one or more lacunae, and assigning a lower boundary value of 0% to the optical density values of those image units; and 5) determining the hematocrit of the sample by assigning relative values to the optical density value of each image of the imaged sample portion as a function of the upper and lower boundary values, and averaging the relative values.

摘要翻译： 提供了一种用于确定血液样本的血细胞比容的方法，其包括以下步骤：1）将样品沉积到适于静态保持样品进行分析的分析室中，由第一和第二面板的内表面限定的腔室和 高度在其间延伸，其中两个面板是透明的，并且高度使得样品中的至少一些红细胞接触面板的两个内表面，并且静止样品内的一个或多个空隙在内表面之间延伸; 2）对静止样品的至少一部分进行成像，该样品部分包含红细胞和一个或多个空隙，以基于每个图像单位确定样品的成像部分的光密度; 3）选择和平均与内表面接触的红细胞对准的图像单元的光密度值，并将上限边界值分配给这些图像单元的平均光密度值; 4）选择与一个或多个空白对齐的图像单元的光密度值，并为这些图像单元的光密度值分配0％的下边界值; 以及5）通过将所述成像样本部分的每个图像的光密度值分配相对值作为上边界值和下边界值的函数来确定样本的血细胞比容，并平均相对值。

公开(公告)号：US07977106B2

公开(公告)日：2011-07-12

申请号：US12785191

申请日：2010-05-21

申请人： Cindra A. Widrig Opalsky ,  David Opalsky ,  Andrzej Maczuszenko ,  Imants R. Lauks ,  Rhonda J. Cheadle

发明人： Cindra A. Widrig Opalsky ,  David Opalsky ,  Andrzej Maczuszenko ,  Imants R. Lauks ,  Rhonda J. Cheadle

IPC分类号： G01N33/86

CPC分类号： C12Q1/56 ,  G01N33/86 ,  Y10T436/11 ,  Y10T436/2575

摘要： This invention is a disposable cartridge for use at the patient side to perform traditional coagulation assays on fresh whole blood or blood derivative samples. The cartridge, in use with an electronic analyzer allows a fluid sample to be metered and quantitatively mixed with reagents which activate the coagulation cascade. An artificial substrate for thrombin, the enzyme whose action results in clot formation is also provided. Clot formation is subsequently detected using a microfabricated sensor also housed within the cartridge which detects electrochemically the product of the thrombin reaction upon the synthetic substrate.

公开(公告)号：US20110157344A1

公开(公告)日：2011-06-30

申请号：US12982489

申请日：2010-12-30

申请人： Min Xie ,  Yang Zhang

发明人： Min Xie ,  Yang Zhang

IPC分类号： H04N7/18

CPC分类号： G06K9/00127 ,  G02B7/38 ,  G02B21/244

摘要： A method and apparatus for imaging a biologic fluid sample quiescently residing within a chamber is provided. The method includes the steps of: a) positioning the chamber at a Z-axis position relative to an objective lens having a lens axis, wherein the Z-axis is parallel to the lens axis; b) moving one or both of the chamber and the objective lens relative to one another at a velocity along the Z-axis; and c) creating one or more images of the biologic fluid sample as one or both of the chamber and the objective lens are moving at a velocity relative to one another within a focus search range along the Z-axis.

摘要翻译： 提供了一种静置在腔室内的生物流体样品成像方法和装置。 该方法包括以下步骤：a）将腔室相对于具有透镜轴的物镜定位在Z轴位置，其中Z轴平行于透镜轴线; b）以沿Z轴的速度相对于彼此移动腔室和物镜中的一个或两个; 以及c）创建所述生物液体样品的一个或多个图像，因为所述腔室和所述物镜中的一个或两个以沿Z轴的焦点搜索范围内的相对于彼此的速度移动。

公开(公告)号：US20110117581A1

公开(公告)日：2011-05-19

申请号：US12620230

申请日：2009-11-17

申请人： John Lewis Emerson Campbell ,  Graham Davis

发明人： John Lewis Emerson Campbell ,  Graham Davis

IPC分类号： G01N33/53 ,  C12M1/34

CPC分类号： G01N33/54313 ,  G01N33/54393 ,  Y10T436/25125

摘要： The invention is directed to methods and devices for reducing interference from leukocytes in an competitive analyte immunoassays. In one embodiment, the invention is to a method comprising the steps of (a) amending a biological sample such as a whole blood sample with sacrificial beads opsonized for leukocytes; and (b) performing a competitive immunoassay on the amended sample to determine the concentration of said analyte in said sample. Preferably, the sample is amended with IgG-coated sacrificial beads.

摘要翻译： 本发明涉及用于在竞争性分析物免疫测定中减少来自白细胞的干扰的方法和装置。 在一个实施方案中，本发明涉及一种方法，包括以下步骤：（a）用生物样品（例如全血样品）修饰用于白细胞调理的牺牲珠; 和（b）对经修改的样品进行竞争性免疫测定以确定所述样品中所述分析物的浓度。 优选地，用IgG涂覆的牺牲珠改变样品。

公开(公告)号：US20110117580A1

公开(公告)日：2011-05-19

申请号：US12620179

申请日：2009-11-17

申请人： John Lewis Emerson Campbell ,  Adam Roger Moss ,  Graham Davis

发明人： John Lewis Emerson Campbell ,  Adam Roger Moss ,  Graham Davis

IPC分类号： G01N33/53 ,  C12M1/34

CPC分类号： G01N33/54313 ,  G01N33/54393 ,  Y10T436/25125

摘要： The invention is directed to methods and devices for reducing interference from leukocytes in an analyte immunoassay, and in particular in non-competitive immunoassays. In one embodiment, the invention is to a method comprising the steps of (a) amending a biological sample such as a whole blood sample with sacrificial beads; and (b) performing a non-competitive immunoassay on the amended sample to determine the concentration of said analyte in said sample. Preferably, the sample is amended with IgG-coated sacrificial beads.

摘要翻译： 本发明涉及用于在分析物免疫测定中特别是在非竞争性免疫测定中减少来自白细胞的干扰的方法和装置。 在一个实施方案中，本发明涉及一种方法，其包括以下步骤：（a）用牺牲珠修饰生物样品例如全血样品; 和（b）对经修改的样品进行非竞争性免疫测定以确定所述样品中所述分析物的浓度。 优选地，用IgG涂覆的牺牲珠改变样品。

公开(公告)号：US07923256B2

公开(公告)日：2011-04-12

申请号：US12785257

申请日：2010-05-21

申请人： Cindra A. Widrig Opalsky ,  David Opalsky ,  Andrzej MacZuszenko ,  Imants R. Lauks ,  Rhonda J. Cheadle

发明人： Cindra A. Widrig Opalsky ,  David Opalsky ,  Andrzej MacZuszenko ,  Imants R. Lauks ,  Rhonda J. Cheadle

IPC分类号： G01N33/86

CPC分类号： C12Q1/56 ,  G01N33/86 ,  Y10T436/11 ,  Y10T436/2575

摘要： This invention is a disposable cartridge for use at the patient side to perform traditional coagulation assays on fresh whole blood or blood derivative samples. The cartridge, in use with an electronic analyzer allows a fluid sample to be metered and quantitatively mixed with reagents which activate the coagulation cascade. An artificial substrate for thrombin, the enzyme whose action results in clot formation is also provided. Clot formation is subsequently detected using a microfabricated sensor also housed within the cartridge which detects electrochemically the product of the thrombin reaction upon the synthetic substrate.

摘要翻译： 本发明是用于患者侧的一次性药筒，用于对新鲜全血或血液衍生物样品进行传统的凝血测定。 与电子分析仪一起使用的墨盒允许将流体样品计量并与激活凝血级联的试剂定量混合。 还提供了用于凝血酶的人造底物，其作用导致凝块形成。 随后使用也容纳在药筒内的微制造传感器检测凝块形成，其中电化学地检测合成基质上凝血酶反应的产物。

公开(公告)号：USD627065S1

公开(公告)日：2010-11-09

申请号：US29339240

申请日：2009-06-26

申请人： Rhonda Ade ,  Bruce Hendry ,  John Campbell

设计人： Rhonda Ade ,  Bruce Hendry ,  John Campbell