公开(公告)号：US20180245086A1

公开(公告)日：2018-08-30

申请号：US15894746

申请日：2018-02-12

Applicant: Andreas Seyfang ,  Christopher L. Massengill ,  Sara R. Sievers

Inventor： Andreas Seyfang ,  Christopher L. Massengill ,  Sara R. Sievers

IPC: C12N15/65 ,  C12N15/79 ,  A61K39/002 ,  C12N15/87 ,  C12N13/00 ,  C12N1/10

CPC classification number: C12N15/65 ,  A61K39/002 ,  A61K2039/51 ,  A61K2039/522 ,  C12N1/10 ,  C12N13/00 ,  C12N15/79 ,  C12N15/87 ,  C12N2830/50

Abstract: Disclosed herein are expression vectors suitable for transfection in amoebas. The vectors may include a promoter from a protein-encoding gene from an amoeba, a selection marker, and a polynucleotide sequence encoding a polypeptide of interest, operably linked to the promoter. The promoter may be from the ACT1 gene from Naegleria fowleri.

公开(公告)号：US20180236102A1

公开(公告)日：2018-08-23

申请号：US15885104

申请日：2018-01-31

Applicant: CureVac AG

Inventor： Mariola FOTIN-MLECZEK ,  Patrick BAUMHOF

IPC: A61K48/00 ,  A61K38/45 ,  A61K39/00 ,  C12N15/87 ,  C07K19/00 ,  C12N9/02 ,  A61K47/64

CPC classification number: A61K48/005 ,  A61K9/0019 ,  A61K38/45 ,  A61K38/4846 ,  A61K39/00 ,  A61K47/61 ,  A61K47/6455 ,  A61K48/0033 ,  A61K48/0041 ,  A61K48/0066 ,  A61K48/0075 ,  A61K2039/53 ,  C07K19/00 ,  C12N9/0069 ,  C12N15/87 ,  C12Y113/12007 ,  C12Y204/01007 ,  C12Y304/21022

Abstract: The present invention relates to a complexed RNA, comprising at least one RNA complexed with one or more oligopeptides, wherein the oligopeptide, which has the function of cell-penetrating peptide (CPP), has a length of 8 to 15 amino acids and has the empirical formula (Arg)i;(Lys)m;(His)n;(Om)o;(Xaa)x with the majority of residues being selected from Arg, Lys, His, Om. The invention further relates to a method for transfecting a cell or an organism, thereby applying the inventive complexed RNA. Additionally, pharmaceutical compositions and kits comprising the inventive complexed RNA, as well as the use of the inventive complexed RNA for transfecting a cell, tissue or an organism and/or for modulating, preferably inducing or enhancing, an immune response are disclosed herein.

公开(公告)号：US20180214485A1

公开(公告)日：2018-08-02

申请号：US15936210

申请日：2018-03-26

Applicant: Sangamo Therapeutics, Inc. ,  Ospedale San Raffaele srl

Inventor： Philip D. Gregory ,  Michael C. Holmes ,  David Paschon ,  Lei Zhang ,  Maria Chiara Bonini ,  Pietro Genovese ,  Zulma Magnani ,  Sara Mastaglio ,  Luigi Naldini

IPC: A61K35/26 ,  C12N15/87 ,  A61K39/00

CPC classification number: A61K35/26 ,  A61K39/0011 ,  A61K2039/5156 ,  A61K2039/5158 ,  C12N15/86 ,  C12N15/87 ,  C12N15/907 ,  C12N2501/515 ,  C12N2510/00 ,  C12N2740/16043

Abstract: Disclosed herein are methods and compositions for modifying TCR genes, using nucleases (zinc finger nucleases or TAL nucleases) to modify TCR genes.

公开(公告)号：US20180201972A1

公开(公告)日：2018-07-19

申请号：US15918357

申请日：2018-03-12

Applicant: Wisconsin Alumni Research Foundation

Inventor： William L. Murphy ,  Siyoung Choi ,  Xiaohua Yu

IPC: C12Q1/68 ,  C12N15/87

CPC classification number: C12Q1/68 ,  C12N15/87

Abstract: Disclosed are methods for cell transfection and regulating cellular behavior. More particularly, the present disclosure relates to methods of non-viral cell transfection and regulating cellular behavior using mineral coatings that allow for the enhanced transfection of cells. The mineral coatings bind polynucleotides and provide a source of calcium and phosphate ions to enhance transfection. The present disclosure also provides a high throughput platform for screening non-viral transfection of cells. The methods of the present disclosure also provide an advantageous polynucleotide delivery platform because the mineral coatings may be deposited on various medical device materials after being specifically developed using the high throughput screening platform.

公开(公告)号：US20180179548A1

公开(公告)日：2018-06-28

申请号：US15840273

申请日：2017-12-13

Applicant: Life Technologies Corporation

Inventor： Farzad HAERIZADEH ,  Todd Peterson ,  Wen Chen ,  Ewa Lis

IPC: C12N15/82 ,  C12N15/79 ,  C12N1/12 ,  C12N15/87 ,  C12N5/00

CPC classification number: C12N15/8206 ,  C12N1/12 ,  C12N5/0025 ,  C12N15/79 ,  C12N15/87

Abstract: The present invention provides compositions and methods for the genetic manipulation of Algal cells. The compositions and methods allow enhanced transfer of genetic material into Algal cells and the cloning and selection of genetically modified cells. Expression of proteins encoded by the genetic material will be enhanced by the methods and compositions of the invention.

公开(公告)号：US20180179525A1

公开(公告)日：2018-06-28

申请号：US15821632

申请日：2017-11-22

Applicant: Flagship Pioneering, Inc.

Inventor： Laura Gabriela Lande ,  David Arthur Berry ,  Rahul Karnik

IPC: C12N15/113 ,  C12N15/63 ,  C12N15/90

CPC classification number: C12N15/113 ,  A01K2217/056 ,  A01K2227/706 ,  A01K2267/0318 ,  A61K35/12 ,  C07K2319/00 ,  C07K2319/85 ,  C12N9/00 ,  C12N9/1007 ,  C12N9/22 ,  C12N9/80 ,  C12N15/63 ,  C12N15/85 ,  C12N15/87 ,  C12N15/90 ,  C12N2310/14 ,  C12N2310/20 ,  C12Y201/01037 ,  C12Y201/01043 ,  C12Y305/01098

Abstract: The present disclosure provides compositions with a modulating gene expression and methods for modulating transcription.

公开(公告)号：US20180169266A1

公开(公告)日：2018-06-21

申请号：US15884618

申请日：2018-01-31

Applicant: CureVac AG

Inventor： Mariola FOTIN-MLECZEK ,  Patrick BAUMHOF

IPC: A61K48/00 ,  C12N15/87 ,  C12N9/02 ,  C07K19/00 ,  A61K47/64 ,  A61K39/00 ,  A61K38/45

CPC classification number: A61K48/005 ,  A61K9/0019 ,  A61K38/45 ,  A61K38/4846 ,  A61K39/00 ,  A61K47/61 ,  A61K47/6455 ,  A61K48/0033 ,  A61K48/0041 ,  A61K48/0066 ,  A61K48/0075 ,  A61K2039/53 ,  C07K19/00 ,  C12N9/0069 ,  C12N15/87 ,  C12Y113/12007 ,  C12Y204/01007 ,  C12Y304/21022

Abstract: The present invention relates to a complexed RNA, comprising at least one RNA complexed with one or more oligopeptides, wherein the oligopeptide, which has the function of cell-penetrating peptide (CPP), has a length of 8 to 15 amino acids and has the empirical formula (Arg)l;(Lys)m;(His)n;(Om)o;(Xaa)x with the majority of residues being selected from Arg, Lys, His, Om. The invention further relates to a method for transfecting a cell or an organism, thereby applying the inventive complexed RNA. Additionally, pharmaceutical compositions and kits comprising the inventive complexed RNA, as well as the use of the inventive complexed RNA for transfecting a cell, tissue or an organism and/or for modulating, preferably inducing or enhancing, an immune response are disclosed herein.

公开(公告)号：US20180163232A1

公开(公告)日：2018-06-14

申请号：US15558794

申请日：2016-03-14

Applicant: INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY, CHINESE ACADEMY OF SCIENCES

Inventor： Caixia GAO ,  Zhen LIANG ,  Yanpeng WANG ,  Qiwei SHAN ,  Qianna SONG

IPC: C12N15/90 ,  C12N15/11 ,  C12N9/96 ,  C12N9/22

CPC classification number: C12N15/902 ,  A01H5/00 ,  C12N9/22 ,  C12N9/96 ,  C12N15/01 ,  C12N15/11 ,  C12N15/8207 ,  C12N15/8213 ,  C12N15/87 ,  C12N2310/20 ,  C12N2800/80

Abstract: The invention disclosed a method for conducting site-directed modification to a plant genome using non-inheritable materials. The method provided in the present invention specifically comprises the following steps: introducing a non-inheritable material into a cell or a tissue or a part of the plant of interest; wherein said non-inheritable material is a nuclease specific to said target fragment or an mRNA expressing said nuclease, thereby the target fragment is cleaved by said nuclease and site-directed modification to the target fragment is achieved through DNA repairing in the plant. By introducing a non-inheritable material of sequence-specific nuclease, site-directed mutation in a plant gene can be achieved, and no exogenous gene or nucleic acid fragments will be integrated into the plant as obtained. Therefore, the present invention can lead to more precise genome function study and higher biosafety in breeding.

公开(公告)号：US20180155669A1

公开(公告)日：2018-06-07

申请号：US15885766

申请日：2018-01-31

Applicant: Indee, Inc.

Inventor： Ryan Pawell

IPC: C12M1/42 ,  C12N15/87 ,  B81B1/00 ,  C12M3/06

CPC classification number: C12M35/04 ,  B81B1/00 ,  B81B2201/058 ,  B81B2203/0338 ,  C12M23/16 ,  C12M27/18 ,  C12M35/00 ,  C12N15/87 ,  C12N2510/00 ,  C12N2521/00 ,  C12N2740/15043

Abstract: Methods for introducing exogenous material into a cell are provided, which include exposing the cell to a transient decrease in pressure in the presence of the exogenous material. Also provided are devices for performing the method of the invention.

公开(公告)号：US09970027B2

公开(公告)日：2018-05-15

申请号：US15665201

申请日：2017-07-31

Applicant: Caribou Biosciences, Inc.

Inventor： Paul Daniel Donohoue ,  Andrew Paul May

IPC: C12N15/87 ,  C12N9/96 ,  C12N9/22 ,  C12N15/113 ,  C12N15/11

CPC classification number: C12N15/87 ,  C12N9/22 ,  C12N9/96 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2310/3513 ,  C12N2310/531

Abstract: The present specification discloses engineered Type II CRISPR-Cas9 systems comprising split-nexus Cas9-associated polynucleotides (sn-casPNs), including systems comprising three split-nexus Cas9-associated polynucleotides (sn1-casPN/sn2-casPN/sn3-casPN) and systems comprising two split-nexus Cas9-associated polynucleotides (sn1-casPN/sn2-casPN). Together with a Cas9 protein, the sn-casPNs facilitate site-specific modifications, including cleavage and mutagenesis, of a target polynucleotide in vitro and in vivo. Furthermore, the engineered Type II CRISPR-Cas9 systems comprising sn-casPNs are useful in methods of regulating expression of a target nucleic acid. Methods are described herein for the creation of a variety of engineered Type II CRISPR-Cas9 systems comprising two or more sn-casPNs. Polynucleotide sequences, expression cassettes, vectors, compositions, and kits for carrying out a variety of methods are also described. Furthermore, the present specification provides genetically modified cells, compositions of modified cells, transgenic organisms, pharmaceutical compositions, as well as a variety of compositions and methods involving the engineered Type II CRISPR-Cas9 systems.