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    MATERNALLY INDUCED STERILITY IN ANIMALS
    51.
    发明申请
    MATERNALLY INDUCED STERILITY IN ANIMALS 有权
    动物中诱发的绝育性

    公开(公告)号：US20120304323A1

    公开(公告)日：2012-11-29

    申请号：US13511317

    申请日：2010-11-23

    申请人： Xavier Lauth ,  John T. Buchanan

    发明人： Xavier Lauth ,  John T. Buchanan

    IPC分类号： C12N15/85

    CPC分类号： A01K67/0275 ,  A01K2207/05 ,  A01K2217/052 ,  A01K2217/206 ,  A01K2227/40 ,  A01K2267/03 ,  C07K14/4747 ,  C12N15/8509 ,  C12N2800/80 ,  Y02A90/40

    摘要： The present invention provides Maternal Sterility Constructs (MSC) and methods of producing sterile progeny lacking germ cells. Female animals carrying the MSC transgene will give rise to a sterile generation, as the MSC specifically eliminates Progenitor Germ Cells (PGCs) of her progeny. These females are called lineage ending females. Male animals carrying the MSC transgene, however, give rise to fertile progeny (assuming the male is not derived from an MSC-transgenic female). Thus, MSC transgenic males can be used to propagate the transgenic line. The invention can be advantageously applied to eliminate pest or invasive species, or to provide effective population control and improve culture performance of farmed species, such as fish and shellfish.

    摘要翻译： 本发明提供产妇无菌构建体（MSC）以及生产缺乏生殖细胞的无菌后代的方法。 携带MSC转基因的雌性动物将产生无菌的代，因为MSC特异性地消除了其后代的祖细胞（PGCs）。 这些女性被称为流血女性。 然而，携带MSC转基因的雄性动物产生可育后代（假设雄性不是源自MSC转基因雌性）。 因此，MSC转基因雄性可以用于繁殖转基因品系。 本发明可有利地应用于消除有害生物或入侵物种，或提供有效的群体控制并改善养殖物种如鱼和贝类的培养性能。

    Isolation, Cloning, Sequencing and Functional analysis of ss-casein promoter along with the regions of exon1, intron1 and exon2 using mammary gland derived cell line of Buffalo (Bubulus bubalis)
    52.
    发明申请
    Isolation, Cloning, Sequencing and Functional analysis of ss-casein promoter along with the regions of exon1, intron1 and exon2 using mammary gland derived cell line of Buffalo (Bubulus bubalis) 审中-公开
    使用乳牛（Bubulus bubalis）的乳腺来源的细胞系，分离，克隆，测序和功能分析ss-酪蛋白启动子以及外显子1，内含子1和外显子2的区域

    公开(公告)号：US20120264814A1

    公开(公告)日：2012-10-18

    申请号：US13162367

    申请日：2011-06-16

    申请人： Subeer Suhash MAJUMDAR ,  Nirmalya Ganguli ,  Abul Usmani

    发明人： Subeer Suhash MAJUMDAR ,  Nirmalya Ganguli ,  Abul Usmani

    IPC分类号： C12N15/85 ,  A61P31/04 ,  A61K48/00

    CPC分类号： C12N15/85 ,  A01K2217/206 ,  A01K2267/01 ,  C07K14/4732 ,  C12N2830/008 ,  C12N2830/85

    摘要： The present invention relates to a method of in vitro isolation of buffalo β-caesin promoter (buCSN2) along with the regions of exon1, intron1 and exon2 from the genomic DNA in vitro (Bubalus bubalis) and its functional activity in using mammary cell line. The novel buffalo β-caesin promoter along with exon1, intron1 and exon2 is isolated and cloned upstream of the Enhanced Green flourescence protein (EGFP) gene and sequenced. The transfection of the DNA construct resulted into production of EGFP protein in mammary cell lines, confirming bioactivity of this newly isolated buffalo promoter sequence. More specifically, the present invention relates to isolation, cloning, sequencing and functional analysis of the buffalo β-casein promoter in vitro using mammary cell line.

    摘要翻译： 本发明涉及一种体外分离水牛和大肠杆菌启动子（buCSN2）的方法以及来自基因组DNA（Bubalus bubalis）的外显子1，内含子1和外显子2的区域及其在使用乳腺细胞系中的功能活性 。 分离和克隆了增强型绿色荧光蛋白（EGFP）基因上游的新一代水牛和大肠杆菌启动子以及外显子1，内含子1和外显子2，并测序。 DNA构建体的转染导致在乳腺细胞系中产生EGFP蛋白，证实了这种新分离的水牛启动子序列的生物活性。 更具体地，本发明涉及使用乳腺细胞系在体外分离，克隆，测序和功能分析水牛和bgr-酪蛋白启动子。

    Transgenic mice over-expressing receptor for advanced glycation endproduct (RAGE) in brain and uses thereof
    58.
    发明授权
    Transgenic mice over-expressing receptor for advanced glycation endproduct (RAGE) in brain and uses thereof 失效
    用于脑中晚期糖基化终末产物（RAGE）的转基因小鼠过表达受体及其用途

    公开(公告)号：US07919670B1

    公开(公告)日：2011-04-05

    申请号：US09638653

    申请日：2000-08-14

    申请人： David M. Stern ,  Ann Marie Schmidt ,  Shi Du Yan

    发明人： David M. Stern ,  Ann Marie Schmidt ,  Shi Du Yan

    IPC分类号： G01N33/00 ,  A01K67/027

    CPC分类号： A01K67/0275 ,  A01K67/0278 ,  A01K2217/052 ,  A01K2217/15 ,  A01K2217/206 ,  A01K2227/105 ,  A01K2267/035 ,  A01K2267/0356 ,  C07K14/70503 ,  C12N15/8509

    摘要： The present invention provides for a transgenic non-human animal whose cells contain a DNA sequence comprising: (a) a nerve tissue specific promoter; and (b) a DNA sequence which encodes a receptor for advanced glycation endproducts (RAGE), wherein the promoter and the DNA sequence which encodes the receptor for advanced glycation endproducts (RAGE) are operatively linked to each other and integrated in the genome of the non-human animal, and wherein said non-human animal exhibits a reduced amount of cerebral tissue infarcted following a transient middle cerebral artery occlusion compared to an identical non-human animal lacking said DNA sequence.

    摘要翻译： 本发明提供一种转基因非人动物，其细胞含有DNA序列，其包含：（a）神经组织特异性启动子; （b）编码晚期糖基化终末产物（RAGE）的受体的DNA序列，其中编码晚期糖基化终产物（RAGE）的受体的启动子和DNA序列可操作地相互连接并整合到 非人动物，并且其中所述非人动物与缺乏所述DNA序列的相同的​​非人动物相比，在短暂的大脑中动脉闭塞之后表现出减少的脑组织数量。