公开(公告)号：US09382568B2

公开(公告)日：2016-07-05

申请号：US13058035

申请日：2009-08-10

Applicant: Vyvyan Clare Salisbury ,  Habib Mahmoud Alloush ,  Margaret Ann Smith ,  Paul John Innocenzi ,  Mark William Ruddock ,  Ashley Diane Martin

Inventor： Vyvyan Clare Salisbury ,  Habib Mahmoud Alloush ,  Margaret Ann Smith ,  Paul John Innocenzi ,  Mark William Ruddock ,  Ashley Diane Martin

IPC: C12Q1/06 ,  C12Q1/66 ,  C12Q1/70 ,  G01N33/53 ,  G01N33/573 ,  C12Q1/02

CPC classification number: C12Q1/025

Abstract: The present invention provides an in vitro method for determining the resistance or sensitivity of a cell line or patient sample to a deoxyribonucleoside kinase-dependent drug, wherein the method comprises the steps of: (i) treating a patient sample or cell line, or a portion thereof, with a deoxyribonucleoside kinase-dependent drug; (ii) lysing the cells of the patient sample or cell line from step (i); (iii) optionally, mixing a portion of the cell lysate from step (ii) with a bioluminescent reporter bacteria incorporating a gene coding for deoxyribonucleoside kinase; (iv) mixing a portion of the cell lysate from (ii) with a bioluminescent reporter bacteria incorporating a gene coding for a deoxyribonucleoside kinase and a deoxyribonucleoside kinase transcription promoter; (v) mixing a portion of the cell lysate from step (ii) with a bioluminescent reporter bacteria incorporating a gene coding for a deoxyribonucleoside kinase, a deoxyribonucleoside kinase transcription promoter and a dephosphorylating agent; and (vi) measuring the bioluminescence of each of the mixtures from steps (iii) to (v), wherein the comparative levels of bioluminescence of each of the mixtures provides a measure of the resistance or sensitivity to the drug.

Abstract translation: 本发明提供了用于确定细胞系或患者样品对脱氧核糖核苷激酶依赖性药物的抗性或敏感性的体外方法，其中所述方法包括以下步骤：（i）治疗患者样品或细胞系，或 部分，与脱氧核糖核苷激酶依赖性药物; （ii）裂解来自步骤（i）的患者样品或细胞系的细胞; （iii）任选地，将来自步骤（ii）的细胞裂解物的一部分与包含编码脱氧核糖核苷激酶的基因的生物发光报道细菌混合; （iv）将来自（ii）的细胞裂解物的一部分与包含编码脱氧核糖核苷激酶和脱氧核糖核苷激酶转录启动子的基因的生物发光报道细菌混合; （v）将来自步骤（ii）的细胞裂解物的一部分与包含编码脱氧核糖核苷激酶的基因的生物发光报道细菌，脱氧核糖核苷激酶转录启动子和去磷酸化剂混合; 和（vi）测量来自步骤（iii）至（v）的每种混合物的生物发光，其中每种混合物的生物发光的比较水平提供对药物的抗性或敏感性的量度。

公开(公告)号：US09222949B2

公开(公告)日：2015-12-29

申请号：US12662651

申请日：2010-04-27

Applicant: Stephen P. Fitzgerald ,  Robert I. McConnell ,  Philip A. Lowry ,  Elouard Benchikh

Inventor： Stephen P. Fitzgerald ,  Robert I. McConnell ,  Philip A. Lowry ,  Elouard Benchikh

IPC: G01N33/53 ,  C07K16/16 ,  G01N33/94

CPC classification number: G01N33/94 ,  C07K16/16 ,  G01N33/946

Abstract: The present invention provides an immunoassay method for detecting or determining the amount of salvinorin A, salvinorin B and/or analogues thereof in an in vitro sample, an antibody for salvinorin A, salvinorin B and/or analogues thereof and a kit for detecting the presence of or determining the amount of salvinorin A, salvinorin B and its analogues thereof in a sample.

Abstract translation: 本发明提供了用于检测或确定体外样品中salvinorin A，salvinorin B和/或其类似物的量的免疫测定方法，salvinorin A的抗体，salvinorin B和/或其类似物，以及用于检测存在的试剂盒 或确定样品中salvinorin A，salvinorin B及其类似物的量。

公开(公告)号：US20140336283A1

公开(公告)日：2014-11-13

申请号：US14367510

申请日：2012-12-21

Applicant: RANDOX LABORATORIES LTD.

Inventor： Sophie Visvikis-Siest ,  Said El Shamieh ,  Helena Murray ,  John Lamont ,  Peter Fitzgerald

IPC: C12Q1/68

Abstract: The invention describes epistatic interactions between single nucleotide polymorphisms on genes associated with blood pressure and provides an application for their use in a method to determine an individual's susceptibility to hypertension and hence whether anti-hypertensive treatment will be beneficial for said individual. In addition gene expression levels are also linked to blood pressure and may also be used to determine susceptibility to hypertension.

Abstract translation: 本发明描述了与血压相关的基因的单核苷酸多态性之间的上位相互作用，并提供了其在确定个体对高血压易感性的方法中的应用，因此抗高血压治疗是否对所述个体有益。 此外，基因表达水平也与血压相关，也可用于确定对高血压的易感性。

公开(公告)号：US08852953B2

公开(公告)日：2014-10-07

申请号：US13384278

申请日：2010-07-16

Applicant: Elouard Benchikh ,  Stephen Peter Fitzgerald ,  Philip Andrew Lowry ,  Ivan Robert McConnell

Inventor： Elouard Benchikh ,  Stephen Peter Fitzgerald ,  Philip Andrew Lowry ,  Ivan Robert McConnell

IPC: G01N33/566 ,  G01N33/53 ,  C07K14/765 ,  C07K16/18 ,  C07D487/04 ,  G01N33/94 ,  C07K16/44

CPC classification number: C07D487/04 ,  C07K16/44 ,  G01N33/948 ,  G01N2430/00

Abstract: The invention relates to novel immunogens, antibodies, methods and kits for use in immunoassays to detect and quantify zaleplon, metabolites of zaleplon and indiplon. These are the first described immunoassays for these compounds and have greater sensitivity than alternative analytical techniques.

Abstract translation: 本发明涉及用于免疫测定中的新型免疫原，抗体，方法和试剂盒，以检测和量化扎来普隆，扎来普隆和独特的代谢物。 这些是对这些化合物的首次描述的免疫测定，并且具有比替代分析技术更大的灵敏度。

公开(公告)号：US08372341B2

公开(公告)日：2013-02-12

申请号：US12087653

申请日：2007-01-25

Applicant: Aiden John Walsh ,  Stephen Peter Fitzgerald

Inventor： Aiden John Walsh ,  Stephen Peter Fitzgerald

IPC: C12Q1/68 ,  G01N21/00 ,  G01N15/06 ,  B01L3/02 ,  B01L9/00 ,  B01D11/02

CPC classification number: G01N35/00029 ,  G01N35/0099 ,  G01N2035/00158 ,  G01N2035/00524 ,  G01N2035/00564 ,  Y10T436/25375 ,  Y10T436/2575

Abstract: Assay device processing apparatus includes a rotatably mounted assay device vessel support; and a drive for rotating the support. The support is rotatable about a substantially horizontal first axis so that, upon rotation, an assay device vessel attached to the support can be inverted.

Abstract translation: 测定装置处理装置包括可旋转地安装的测定装置容器支架; 以及用于旋转支撑件的驱动器。 支撑件可围绕基本上水平的第一轴线旋转，使得在旋转时，附接到支撑件的测定装置容器可以反转。

公开(公告)号：US20120135434A1

公开(公告)日：2012-05-31

申请号：US13377522

申请日：2010-05-26

Applicant: Elouard Benchikh ,  Stephen Peter Fitzgerald ,  Paul John Innocenzi ,  Philip Andrew Lowry ,  Ivan Robert McConnell

Inventor： Elouard Benchikh ,  Stephen Peter Fitzgerald ,  Paul John Innocenzi ,  Philip Andrew Lowry ,  Ivan Robert McConnell

IPC: G01N33/53

CPC classification number: G01N33/9466

Abstract: The invention describes a practical and robust multi-antibody approach to the sensitive immunodetection and determination of the drug of abuse m-chlorophenyl piperazine (mCPP). The invention also describes methods and kits for mCPP detection in an in vitro sample.

Abstract translation: 本发明描述了用于敏感免疫检测和滥用间氯苯哌嗪（mCPP）药物的测定的实用和强大的多抗体方法。 本发明还描述了在体外样品中用于mCPP检测的方法和试剂盒。

公开(公告)号：US07507584B2

公开(公告)日：2009-03-24

申请号：US11587297

申请日：2005-04-14

Applicant: Marie Asberg ,  Ake Nygren ,  Rolf Ekman

Inventor： Marie Asberg ,  Ake Nygren ,  Rolf Ekman

IPC: G01N33/00 ,  G01N33/48

CPC classification number: G01N33/74 ,  G01N33/6863 ,  G01N2333/475 ,  G01N2333/485 ,  G01N2333/52 ,  G01N2333/54 ,  G01N2333/59

Abstract: Methods for diagnosing chronic stress in patients by first determining the level of one or more analytes in a patient sample and establishing the significance of the one or more analyte levels.

Abstract translation: 通过首先确定患者样品中一种或多种分析物的水平并确定一种或多种分析物水平的显着性来诊断患者的慢性应激的方法。

公开(公告)号：US20080318229A1

公开(公告)日：2008-12-25

申请号：US11922229

申请日：2006-06-19

Applicant: Maria Zellner ,  Rudolf Oehler

Inventor： Maria Zellner ,  Rudolf Oehler

IPC: C12Q1/68 ,  C40B40/10 ,  C12Q1/02 ,  G01N33/53 ,  C12Q1/24

CPC classification number: G01N33/6896 ,  C07K14/4711 ,  C12Q1/6883 ,  C12Q2600/158 ,  G01N2333/90638 ,  G01N2800/2814 ,  G01N2800/2821 ,  G01N2800/2835

Abstract: A method for the diagnosis of Alzheimer's Disease (AD) or Parkinson's Disease (PD), comprises measuring the level of expression of one or more AD markers (Table 1) or PD markers (Table 2) in a sample of platelets isolated from a person suspected of having AD or PD, and determining whether the levels of expression are altered compared to a control.

Abstract translation: 用于诊断阿尔茨海默病（AD）或帕金森病（PD）的方法包括测量从人分离的血小板样品中的一种或多种AD标志物（表1）或PD标志物（表2）的表达水平 怀疑患有AD或PD，并确定与对照相比，表达水平是否改变。

公开(公告)号：US12246323B2

公开(公告)日：2025-03-11

申请号：US17602826

申请日：2020-04-09

Applicant: RANDOX LABORATORIES LTD

Inventor： Marc Wharry ,  Martin Reid ,  Peter Fitzgerald

IPC: B01L3/00 ,  B01L9/00

Abstract: An assay cartridge for use in a molecular diagnostics assay having an assay region; a first end comprising a first engagement member for engaging with a support housing; and a second end comprising a second engagement member for engaging with a support housing. The second engagement member has a clip, the clip being formed by a resilient element extending from the second end of the cartridge and a fastening element extending from said resilient element. The clip has a release element extending from the resilient element actuatable to release the clip from a caddy in use.

公开(公告)号：US12214102B2

公开(公告)日：2025-02-04

申请号：US17602853

申请日：2020-04-09

Applicant: RANDOX LABORATORIES LTD

Inventor： Catherine McCooke ,  Peter Fitzgerald

IPC: A61L2/28 ,  A61L2/10 ,  G01J1/42 ,  G01J1/44

Abstract: There is provided a sterilisation monitoring device for a molecular diagnostics analyser. The device comprises a sensor arranged in use to detect radiation; and an analysis unit connected to the sensor. The analysis unit is arranged in use to issue a signal when the radiation level incident on the sensor is lower than a threshold level.