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公开(公告)号：US20160153868A1
公开(公告)日：2016-06-02
申请号：US14557169
申请日：2014-12-01
Applicant: GENERAL ELECTRIC COMPANY
Inventor： John Richard Nelson , Wei Gao , Christopher Michael Puleo , Todd Frederick Miller , Christine Lynne Pitner , David Andrew Shoudy , Alex David Corwin
IPC: G01N1/10
CPC classification number: C12N15/1013 , B01L3/502 , B01L2200/025 , B01L2200/0689 , B01L2300/06 , B01L2300/0654 , B01L2300/0861 , C12Q1/6806 , G01N1/02 , G01N1/10 , G01N1/40 , G01N2001/028 , G01N2001/4038
Abstract: A system for extracting material from a region of interest includes a fluid delivery base comprising an inlet channel and an outlet channel formed within the fluid delivery base; a gasket affixed to the fluid delivery base, wherein the gasket comprises at least one opening exposing an open end of the inlet channel and an open end of the outlet channel; a support comprising a sample-supporting surface facing the gasket and an opposing surface; and an alignment member coupled to the opposing surface in a fixed position and such that the support is positioned between the fluid delivery base and the alignment member, wherein one or both of the alignment member or the fluid delivery base are biased towards one another by a force (e.g., a magnet or spring force) and wherein the fluid delivery base is separable from the support and configured to move along a plane of the sample-supporting surface to align with the alignment member.
Abstract translation: 用于从感兴趣区域提取材料的系统包括流体输送基底，其包括形成在流体输送基底内的入口通道和出口通道; 固定到流体输送基座上的垫圈，其中垫圈包括暴露入口通道的开口端和出口通道的开口端的至少一个开口; 支撑件，其包括面向所述垫圈的样品支撑表面和相对表面; 以及对准构件，其在固定位置联接到所述相对表面，并且使得所述支撑件定位在所述流体输送基座和所述对准构件之间，其中所述对准构件或所述流体输送基座中的一个或两个被朝向彼此偏置 力（例如，磁体或弹簧力），并且其中流体输送基座可与支撑件分离并且构造成沿着样品支撑表面的平面移动以与对准构件对准。
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公开(公告)号：US09333463B2
公开(公告)日：2016-05-10
申请号：US13951929
申请日：2013-07-26
Applicant: General Electric Company
Inventor： Christopher Michael Puleo , John Richard Nelson , Patrick McCoy Spooner , Ralf Lenigk , Nichole Lea Wood , Li Zhu , Craig Patrick Galligan
IPC: G01N27/447 , B01D57/02
CPC classification number: B01D57/02 , G01N27/4473
Abstract: A device and a system for eluting biomolecules from biological sample by electroelution are provided. The device for electroelution of biomolecules from a biological sample is constituted with a housing configured to receive an electrolyte and the biological sample, at least two electrodes comprising conductive redox polymers operationally coupled to the housing, and a biomolecule impermeable layer disposed on at least one of the electrodes. The biomolecule impermeable layer disposed on at least one of the electrodes to prevent the biomolecules from reaching the electrode. A system is provided, wherein the system comprises a sample collection port, one or more reservoirs comprising a buffer, a solvent, a reagent or combinations thereof, an device for electroelution, and a controller.
Abstract translation: 提供了一种通过电洗脱从生物样品中洗脱生物分子的装置和系统。 用于从生物样品电洗脱生物分子的装置由构造成接收电解质和生物样品的壳体构成，至少两个电极包括可操作地耦合到壳体的导电氧化还原聚合物，以及生物分子不可渗透层，其设置在至少一个 电极。 生物分子不渗透层设置在至少一个电极上以防止生物分子到达电极。 提供了一种系统，其中系统包括样品采集端口，一个或多个包含缓冲液，溶剂，试剂或其组合的储存器，用于电洗脱的装置和控制器。
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53.发明申请公开(公告)号：US20160123926A1
公开(公告)日：2016-05-05
申请号：US14865478
申请日：2015-09-25
Applicant: General Electric Company
Inventor： John Richard Nelson , Christopher Michael Puleo , Erin Jean Finehout , Patrick McCoy Spooner , Kashan Ali Shaikh , Xiaohui Chen , Li Zhu
IPC: G01N27/447
CPC classification number: G01N27/44791 , C07H1/08 , C07H21/00 , C12N15/1017 , G01N27/44747
Abstract: A method of isolating nucleic acids from a biological material, comprises applying the biological material on a substrate comprising one or more cell lysis reagents impregnated therein; applying a fluid to the biological material applied on the substrate; extracting the nucleic acids from the biological material applied on the substrate; and collecting the extracted nucleic acids in a substantially intact form, wherein the collected nucleic acid has a molecular weight greater than or equal to 20 kb.
Abstract translation: 从生物材料中分离核酸的方法包括将生物材料应用于包含其中浸渍的一种或多种细胞裂解试剂的底物; 将液体施加到施加在基底上的生物材料; 从施加在基底上的生物材料中提取核酸; 并以基本上完整的形式收集所提取的核酸，其中所收集的核酸具有大于或等于20kb的分子量。
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54.发明申请公开(公告)号：US20150166956A1
公开(公告)日：2015-06-18
申请号：US14167393
申请日：2014-01-29
Applicant: General Electric Company
Inventor： Christopher Michael Puleo , Erik Leeming Kvam , Gregory Andrew Grossmann , Craig Patrick Galligan , Jason Michael Nichols , Xuefeng Wang , Jason Louis Davis
IPC: C12N5/078
CPC classification number: B01D21/0087 , B01D17/02 , B01D21/0006 , B01D21/0042 , B01D21/02 , B01D21/10 , B01D21/2433 , B01D21/2444 , B01D21/245 , B01D2221/04 , B01L3/502753 , B01L3/502761 , B01L2200/0631 , B01L2200/0652 , B01L2200/0668 , B01L2300/0681 , B01L2300/0851 , B01L2400/0421 , B01L2400/043 , B01L2400/0457 , B01L2400/0469 , C02F1/00 , C02F1/24 , C02F1/444 , C02F1/48 , C02F2001/007 , C02F2101/32 , C02F2103/08 , C02F2103/10 , C02F2201/002 , C12M47/02 , C12N5/0641
Abstract: A device is configured for separation of particulates dispersed within a base fluid, wherein the particulates have a relative density difference compared to the base fluid. The device comprises a microchannel of length l and height h comprising an inlet and an outlet; a microporous surface on one or more walls of the microchannel; a collection chamber on an opposing side of the microporous surface; and an applied force field across the height h of the microchannel to sediment the particles through the microporous surface into the collection chamber. The microporous body operationally generates a fluid flow regime comprising a first fluid flow having a first flow rate through the microchannel and a second fluid flow having a second flow rate through the collection chamber and the second flow rate is a fraction of the first flow rate.
Abstract translation: 一种装置被配置用于分离分散在基础流体中的微粒，其中所述颗粒与基础流体相比具有相对密度差。 该装置包括长度为l的微通道和包括入口和出口的高度h; 在微通道的一个或多个壁上的微孔表面; 在微孔表面的相对侧上的收集室; 以及穿过微通道的高度h的施加的力场，以将颗粒通过微孔表面沉淀到收集室中。 微孔体可操作地产生流体流动状态，其包括具有通过微通道的第一流量的第一流体流和具有通过收集室的第二流速的第二流体流，第二流量是第一流量的一部分。
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公开(公告)号：US20150027891A1
公开(公告)日：2015-01-29
申请号：US13951881
申请日：2013-07-26
Applicant: General Electric Company
Inventor： Christopher Michael Puleo , John Richard Nelson , Patrick McCoy Spooner , Ralf Lenigk , Nichole Lea Wood , Li Zhu , Craig Patrick Galligan
IPC: B01D57/02
CPC classification number: B01D57/02 , C12N15/101
Abstract: A method of eluting biomolecules, such as nucleic acids from a biological sample by electroelution is provided. An example of a method includes various steps, such as loading the biological sample to a device comprising a housing, at least two conductive redox polymer electrodes operationally coupled to the housing and a biomolecule impermeable layer disposed on at least one of the electrodes. The loading of sample is followed by initiating an electrical connection to generate an electric field strength sufficient to elute biomolecules from the biological sample; and eluting the biomolecules from the biological sample.
Abstract translation: 提供了通过电洗脱来从生物样品中洗脱生物分子如核酸的方法。 方法的实例包括各种步骤，例如将生物样品装载到包括壳体的装置中，至少两个可操作地耦合到壳体的导电氧化还原聚合物电极和设置在至少一个电极上的生物分子不可渗透层。 样品的负载之后是引发电连接以产生足以从生物样品中洗脱生物分子的电场强度; 并从生物样品中洗脱生物分子。
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Patent Agency Ranking
公开(公告)号：US12150984B2
公开(公告)日：2024-11-26
申请号：US17244642
申请日：2021-04-29
Applicant: General Electric Company
IPC: C12N13/00 , A61K39/215 , C07K14/54 , C12N15/86 , A61K39/00
Abstract: The subject matter of the present disclosure generally relates to techniques for addressing or correcting dysregulation of the trauma regulation pathway. The dysregulation may be associated with a physiological condition, such as a SARS-CoV-2 viral infection. In an embodiment, the techniques include treating dysregulation based on a renin-angiotensin pathway molecule or cell and/or a splenic pathway molecule or cell using targeted neuromodulation. In an embodiment, neuromodulation is used to regulate the immune system, e.g., as an energy-based adjuvant for a vaccine.
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公开(公告)号：US11938348B2
公开(公告)日：2024-03-26
申请号：US16978987
申请日：2019-03-06
Applicant: General Electric Company
Inventor： Christopher Michael Puleo , Victoria Eugenia Cotero
CPC classification number: A61N7/00 , A61B5/14532 , A61B5/4836 , A61N2007/0026 , A61N2007/0052 , A61N2007/0073
Abstract: The subject matter of the present disclosure generally relates to techniques for neuromodulation of a tissue (e.g., an organ) that include applying energy (e.g., ultrasound energy) into the tissue to cause altered activity at a synapse between a neuron and a non-neuronal cell. In one embodiment, the energy is applied to cause competing or opposing effects for bi-directional control of physiological processes.
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公开(公告)号：US11786760B2
公开(公告)日：2023-10-17
申请号：US16709717
申请日：2019-12-10
Applicant: General Electric Company
Inventor： John Frederick Graf , Christopher Michael Puleo , Jeffrey Michael Ashe , Victoria Eugenia Cotero , David Andrew Shoudy
IPC: A61N7/00
CPC classification number: A61N7/00 , A61N2007/0026 , A61N2007/0052 , A61N2007/0086
Abstract: The subject matter of the present disclosure generally relates to techniques for neuromodulation of a tissue that include applying energy (e.g., ultrasound energy) into the tissue at multiple regions of interest, concurrently or consecutively. The neuromodulation may result in tissue displacement, which may be observed through changes in one or more molecules of interest.
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公开(公告)号：US11660474B2
公开(公告)日：2023-05-30
申请号：US17208547
申请日：2021-03-22
Applicant: General Electric Company
Inventor： Christopher Michael Puleo , Victoria Eugenia Cotero
CPC classification number: A61N7/00 , A61N2007/0026
Abstract: The subject matter of the present disclosure generally relates to techniques for applying mechanical or ultrasound energy to a region of interest in a subject to induce modulation of one or more nerve pathways. The region of interest may include at least a portion of a nerve ganglion.
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公开(公告)号：US20220362587A1
公开(公告)日：2022-11-17
申请号：US17864212
申请日：2022-07-13
Applicant: General Electric Company
Inventor： Christopher Michael Puleo , Victoria Eugenia Cotero , Jeffrey Michael Ashe , John Frederick Graf
Abstract: Embodiments of the present disclosure relate to techniques for inducing physiological perturbations in a subject via neuromodulation, e.g., peripheral neuromodulation of a region of interest of an organ. The nature and degree of the perturbations may be related to the subject's clinical condition. Accordingly, an assessment of one or more characteristics of the perturbations may be used to determine a clinical condition of the subject.
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