公开(公告)号：US11155862B2

公开(公告)日：2021-10-26

申请号：US16481938

申请日：2018-03-28

申请人： Genetron Health (Beijing) Co., Ltd.

发明人： Hai Yan ,  Sizhen Wang ,  Yuchen Jiao ,  Dayong Xu ,  Qiaosong Zheng ,  Xiao Shi

IPC分类号： C12Q1/6869 ,  C12N15/10 ,  C12Q1/686 ,  C40B50/06

摘要： The present invention discloses a method for rapidly constructing amplicon library including the following steps: 1. Synthesizing a primer combination for constructing an amplicon library of a DNA sample, the primer combination of the amplicon library used to construct the DNA sample includes: a forward fusion primer designed according to the target amplicon, a reverse fusion primer designed according to the target amplicon, a forward universal primer and a reverse universal primer; 2. Constructing a PCR reaction system for the DNA sample; 3. Performing PCR. The method according to the present invention can be used to construct an amplicon library in a simple and rapid manner, and since a barcode is introduced before the start of PCR, the possibility of cross-contamination between the sample and the library is greatly reduced.

公开(公告)号：US11149309B2

公开(公告)日：2021-10-19

申请号：US15695548

申请日：2017-09-05

申请人： IsoPlexis Corporation

发明人： Steven Gordon ,  Daniel Stetson

IPC分类号： C12Q1/6874 ,  B01L3/00 ,  C12Q1/6837 ,  C12Q1/686 ,  C12Q1/6869 ,  C40B50/06 ,  B01L7/00

摘要： The present invention relates to methods and devices for amplifying nucleic acid, and, in particular, amplifying so as to generate products on a surface without the use of emulsions. In a preferred embodiment, a plurality of groups of amplified product are generated on the surface, each group positioned in different (typically predetermined) locations on said surface so as to create an array.

公开(公告)号：US11104721B2

公开(公告)日：2021-08-31

申请号：US16132663

申请日：2018-09-17

申请人： Merck Sharp & Dohme Corp.

发明人： Dongxing Zha ,  Hussam Hisham Shaheen

IPC分类号： C40B50/06 ,  C07K16/00 ,  C07K16/32 ,  C07K16/40 ,  G01N33/68 ,  C40B30/04

摘要： The present invention provides, in part, an antibody display system that simultaneously uses a secretion and a display mode. A bait complexed with a monovalent antibody fragment can be expressed on the surface of the host cell wherein the fragment may be assayed for antigen binding while full antibody is simultaneously secreted from the host cell. Methods of using the system for identifying antibodies that bind specifically to an antigen of interest are also provided. Polypeptides, polynucleotides and host cells useful for making the antibody display system are also provided along with methods of use thereof.

公开(公告)号：US20210254157A1

公开(公告)日：2021-08-19

申请号：US17307912

申请日：2021-05-04

申请人： Roche Sequencing Solutions, Inc.

发明人： Jan BERKA ,  Brian GODWIN ,  Carolina DALLETT ,  Sedide OZTURK ,  Joseph PLATZER ,  Seoyoung KIM

IPC分类号： C12Q1/6881 ,  C12Q1/68 ,  C40B50/06 ,  C12N15/10 ,  C12Q1/6886

摘要： Methods and compositions are described herein for primer extension target enrichment of immune receptor (BCR or TCR) sequences.

公开(公告)号：US20210246504A1

公开(公告)日：2021-08-12

申请号：US17235837

申请日：2021-04-20

申请人： Fred Hutchinson Cancer Research Center

发明人： Jason H. BIELAS

IPC分类号： C12Q1/6874 ,  C12Q1/6869 ,  C12N15/10 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C40B40/08 ,  C40B50/06 ,  C12Q1/6827

摘要： The present disclosure provides compositions and methods for accurately detecting mutations by uniquely tagging double stranded nucleic acid molecules with dual cyphers such that sequence data obtained from a sense strand can be linked to sequence data obtained from an anti-sense strand when sequenced, for example, by massively parallel sequencing methods.

公开(公告)号：US11066662B2

公开(公告)日：2021-07-20

申请号：US16382892

申请日：2019-04-12

申请人： AxioMx, Inc.

发明人： Michael Weiner ,  Margaret Kiss

IPC分类号： C40B50/06 ,  C12N15/10 ,  C07K16/00 ,  C12N15/90 ,  C40B30/04

摘要： Disclosed herein is an efficient method of generating a library of variants of a sequence of interest, such as may be used in directed evolution, in one embodiment, the method includes an amplification reaction, e.g. error-prone PCR, to generate double-stranded DNA (dsDNA) variants of a sequence of interest, after which one strand of the dsDNA variants may be selectively degraded to produce single-stranded DNA (ssDNA) variants. The ssDNA variants may be hybridized to ssDNA intermediaries, e.g., uracilated circular ssDNA intermediaries, to form heteroduplex DNA, which may be transformed into cells, such as E. coli cells, yielding a library of variants. This method eliminates the inefficient sub-cloning steps and the need for costly primer sets required by many prior methods.

公开(公告)号：US20210207129A1

公开(公告)日：2021-07-08

申请号：US17159936

申请日：2021-01-27

申请人： BioAtla, Inc.

发明人： Jay Milton Short

IPC分类号： C12N15/10 ,  C40B50/06 ,  C07K16/00

摘要： The present disclosure provides methods of integrating therapeutic protein and antibody generation and/or selection, evolution and expression in a eukaryotic host for manufacturing in a single system. Therapeutic proteins, including antibodies, are generated, optimized and manufactured in the same eukaryotic host system. The disclosed system of Comprehensive Integrated Antibody Optimization (CIAO!™) allows for simultaneous evolution of protein performance and expression optimization.

公开(公告)号：US11053543B2

公开(公告)日：2021-07-06

申请号：US16885089

申请日：2020-05-27

申请人： GigaGen, Inc.

发明人： David Scott Johnson ,  Everett Hurteau Meyer

IPC分类号： C12Q1/6874 ,  C12N15/10 ,  C12Q1/6844 ,  C12Q1/6886 ,  C12Q1/6881 ,  C12Q1/6837 ,  C12Q1/6883 ,  C12Q1/6888 ,  C40B50/06 ,  C07K16/00

摘要： Methods and systems are provided for massively parallel genetic analysis of single cells in emulsion droplets or reaction containers. Genetic loci of interest are targeted in a single cell using a set of probes, and a fusion complex is formed by molecular linkage and amplification techniques. Methods are provided for high-throughput, massively parallel analysis of the fusion complex in a single cell in a population of at least 10,000 cells. Also provided are methods for tracing genetic information back to a cell using barcode sequences.

公开(公告)号：US20210198661A1

公开(公告)日：2021-07-01

申请号：US17192578

申请日：2021-03-04

申请人： DNA Script

发明人： Xavier Godron ,  Adrian Horgan ,  Sylvain Gariel ,  Jeffrey Jeddaloh ,  Robert Nicol ,  Thomas Ybert

IPC分类号： C12N15/10 ,  C12N15/11 ,  C12Q1/44 ,  C40B50/06 ,  C40B50/08 ,  C40B50/14 ,  C40B70/00

摘要： The invention is directed to methods for synthesizing oligonucleotides direction on biomolecules or cells living or fixed. In some embodiments, template-free enzymatic synthesis is implemented under biological conditions with successive cycles of (i) enzymatic addition of a 3′-O-blocked nucleoside triphosphate and (ii) enzymatic deblocking of the incorporated nucleotide to regenerate a free 3′ hydroxyl. The invention has applications in single-cell cDNA library construction and analysis.

公开(公告)号：US20210164047A1

公开(公告)日：2021-06-03

申请号：US17248722

申请日：2021-02-04

申请人： Life Technologies Corporation

发明人： Timothy LOONEY ,  Geoffrey LOWMAN ,  Lifeng LIN

IPC分类号： C12Q1/6876 ,  C12Q1/6869 ,  C12Q1/6844 ,  C12Q1/6811 ,  C12Q1/6881 ,  C12Q1/6883 ,  C40B50/06

摘要： The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, target-specific primer panels provide for the effective amplification of sequences of T cell receptor and/or B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Variable regions associated with the immune cell receptor are resolved to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.