公开(公告)号：US10927149B2

公开(公告)日：2021-02-23

申请号：US15773381

申请日：2016-10-26

Applicant: BIOLOGICAL E LIMITED

Inventor： Akshay Goel ,  Tushar Joglekar ,  Krishnanand Tiwari ,  Yogesh Mishra ,  Narender Dev Mantena ,  Mahima Datla

IPC: A61K39/00 ,  C12N15/70 ,  C07K1/18 ,  C07K14/005 ,  C07K1/113 ,  C07K14/195 ,  C07K1/12 ,  C07K14/22

Abstract: The present invention relates to a preparation method which is performed by expressing the recombinant carrier proteins in Escherichia coli and purification thereof. More particular, the invention relates to industrially scalable process for the recovery of recombinant carrier proteins.

公开(公告)号：US20210017222A1

公开(公告)日：2021-01-21

申请号：US17042872

申请日：2019-03-29

Applicant: AbbVie Inc.

Inventor： Anthony R Haight ,  Xiaoli Liao

IPC: C07K1/113 ,  C07K16/00 ,  C07K16/28 ,  A61K47/68

Abstract: The present disclosure provides methods for selectively reducing an antibody, comprising contacting the antibody with a reducing agent selected from the group consisting of 2-[2-(Diphenylphosphino)ethyl]pyridine, 3-(Diphenylphosphino)benzenesulfonic acid, 4-(Diphenylphosphino)benzoic acid, 2-(Diphenylphosphino)ethylamine, 3-(diphenylphosphino)propylamine, 3-(Diphenylphosphino)propionic acid, 2-(diisopropylphosphino)ethylamine, 2-(diphenylphosphino)benzoic acid, (2-hydroxyphenyl)diphenylphosphine, and salts thereof. The present disclosure further provides methods of producing antibody conjugates, such as antibody-drug conjugates.

公开(公告)号：US20200347097A1

公开(公告)日：2020-11-05

申请号：US16781233

申请日：2020-02-04

Applicant: ONKURE, INC.

Inventor： Anthony D. Piscopio ,  Xiaoyong Fu ,  Feng Shi ,  Huayan Liu ,  Zhifeng Li

IPC: C07K11/02 ,  C07K5/097 ,  C07D498/06 ,  C07D513/06 ,  C07D513/14 ,  C07K11/00 ,  A61K38/15 ,  C07K1/113

Abstract: Processes for preparing compounds of Formula (1) and Formula (2) are described, wherein X, Y, Z, R1-R7, L and n are defined herein. Intermediates useful in the preparation of the compounds of Formula (1) and Formula (2) are also described.

公开(公告)号：US10822372B2

公开(公告)日：2020-11-03

申请号：US15563871

申请日：2016-03-30

Applicant: Roquette Freres

Inventor： Merveille Nono ,  Nicolas Descamps ,  Denis Simon ,  Olaf Haeusler

IPC: C07K1/113 ,  C12N9/24

Abstract: One subject of the present invention is the use of a combination of a polyol (i) consisting of 12 carbon atoms and of a polyol (ii) consisting of 4 to 6 carbon atoms, as protein stabilizer. Another subject of the present invention is protein compositions comprising such a combination. A final subject of the present invention is a process for preparing protein compositions using such a combination.

公开(公告)号：US20200299402A1

公开(公告)日：2020-09-24

申请号：US16842864

申请日：2020-04-08

Applicant: GENZYME CORPORATION

Inventor： Kevin P. Brower ,  Chris Hwang ,  Rao Koduri ,  Konstantin B. Konstantinov ,  Veena Warikoo ,  Jin Yin ,  Marcella Yu

IPC: C07K16/28 ,  C07K16/00 ,  C07K1/113 ,  A61K39/395 ,  C07K16/22

Abstract: Disclosed herein are methods that have been developed to control the formation of disulfide bonds between polypeptides of a multimeric protein produced by a bioprocess. Also disclosed are protein solution parameters that allow for controlling the formation of disulfide bonds. In one example, the methods disclosed herein can be used to control the proportion of half antibody molecules in an antibody solution.

公开(公告)号：US10590407B2

公开(公告)日：2020-03-17

申请号：US15306249

申请日：2015-03-24

Applicant: NANYANG TECHNOLOGICAL UNIVERSITY

Inventor： Kien Truc Giang Nguyen ,  James P. Tam

IPC: C12N9/88 ,  C12N9/00 ,  C12N11/02 ,  C12N11/06 ,  C12P21/02 ,  C07K1/10 ,  C07K1/113

Abstract: The present invention is directed to enzymes having Asx-specific ligase and cyclase activity and to nucleic acids encoding those as well as methods of the manufacture of said enzymes. Further encompassed are methods and uses of these enzymes.

公开(公告)号：US20200069811A1

公开(公告)日：2020-03-05

申请号：US16674099

申请日：2019-11-05

Applicant: Ariel-University Research and Development Company Ltd.

Inventor： Gary GELLERMAN

IPC: A61K47/64 ,  C07K1/107 ,  C07K1/113 ,  A61K47/65 ,  C07K2/00

Abstract: A molecular structure comprising a targeting moiety, a multi-functional peptide platform and a plurality of controllably released bioactive agents attached thereto is provided herein.

公开(公告)号：US20190262501A1

公开(公告)日：2019-08-29

申请号：US16412884

申请日：2019-05-15

Applicant: Orthocell Pty Ltd.

Inventor： Ming Hao Zheng

IPC: A61L27/24 ,  C07K14/78 ,  A61L27/56 ,  A61L27/36 ,  C07K1/113 ,  A61L27/58

Abstract: The present invention relates to a method of producing a collagen membrane that has particular mechanical properties. In particular, the present invention relates to a method A of producing a collagen membrane comprising the steps of (i) isolating a collagen-containing tissue and incubating same in an ethanol solution; (ii) incubating the collagen-containing tissue from step (i) in a first solution comprising an inorganic salt and an anionic surfactant in order to denature non-collagenous proteins contained therein; (iii) incubating the collagen-containing tissue produced in step (ii) in a second solution comprising an inorganic acid until the collagen in said material is denatured; and (iv) incubating the collagen-containing tissue produced in step (iii) in a third solution comprising an inorganic acid with simultaneous mechanical stimulation for sufficient time to enable the collagen bundles in said collagen-containing tissue to align; wherein the mechanical stimulation comprises applying tension cyclically to the collagen-containing tissue.

公开(公告)号：US10358475B1

公开(公告)日：2019-07-23

申请号：US15997728

申请日：2018-06-05

Applicant: Jose A. Lasalde-Dominicci ,  Orestes Quesada-Gonzalez ,  Josue Rodriguez-Cordero ,  Carlos Baez-Pagan

Inventor： Jose A. Lasalde-Dominicci ,  Orestes Quesada-Gonzalez ,  Josue Rodriguez-Cordero ,  Carlos Baez-Pagan

IPC: C30B29/54 ,  C07K14/705 ,  C07K1/113 ,  C12N9/54

Abstract: The invention is a high-throughput voltage screening crystallographic device and methodology that uses multiple micro wells and electric circuits capable of assaying different crystallization condition for the same or different proteins of interest at the same of different voltages under a humidity and temperature controlled environment. The protein is solubilized in a lipid matrix similar to the lipid composition of the protein in the native environment to ensure stability of the protein during crystallization. The invention provides a system and method where the protein is transferred to a lipid matrix that holds a resting membrane potential, which reduces the degree of conformational freedom of the protein. The invention overcomes the majority of the difficulties associated with vapor diffusion techniques and essentially reconstitutes the protein in its native lipid environment under “cuasi” physiological conditions.

公开(公告)号：US20190169230A1

公开(公告)日：2019-06-06

申请号：US16323247

申请日：2017-08-17

Applicant: ProZyme, Inc.

Inventor： Michael J. KIMZEY ,  Francis T. HAXO ,  Vaishali SHARMA

IPC: C07K1/113 ,  C11D1/62

Abstract: Ammonium cation detergents comprising a quaternary or tertiary ammonium cation can be used as detergents to denature proteins and are particularly useful in denaturing glycoproteins or glycopeptides prior to enzymatic deglycosylation. Ammonium cation detergents with sulfate or sulfonate anions are particularly useful.