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    Transgenic fish and uses thereof
    36.
    发明授权
    Transgenic fish and uses thereof 有权
    转基因鱼及其用途

    公开(公告)号：US09043995B2

    公开(公告)日：2015-06-02

    申请号：US13769533

    申请日：2013-02-18

    申请人： CITY UNIVERSITY OF HONG KONG

    发明人： Shuk Han Cheng ,  Xue Ping Chen

    IPC分类号： C07H21/02 ,  A01K15/00 ,  A01K67/027 ,  C12N15/63 ,  C12N15/85

    CPC分类号： A01K67/0275 ,  A01K2217/05 ,  A01K2217/052 ,  A01K2217/203 ,  A01K2217/206 ,  A01K2227/40 ,  A01K2267/0393 ,  C12N15/63 ,  C12N15/8509 ,  C12N2800/106 ,  C12N2830/002 ,  C12N2830/008

    摘要： The present invention relates to a transgenic fish having at least one genomically integrated expression cassette containing a 5′-regulatory nucleotide sequence responsive to hormones, particularly estrogenic hormones, connected in a functional manner upstream of a nucleotide sequence encoding a reporter protein. The present invention further relates to methods of using the transgenic fish for various purposes, including, for example: (1) identifying estrogenic endocrine disruptors; (2) monitoring estrogen-like activity of test samples; (3) identifying anti-estrogenic endocrine disruptors; and (4) investigating the effects of endocrine disruptors on liver regeneration. Expression cassettes, host cells, and transgenic cells of aquatic animals are also disclosed.

    摘要翻译： 本发明涉及具有至少一种基因整合的表达盒的转基因鱼，所述表达盒含有对编码报道蛋白的核苷酸序列上游功能性方式连接的激素（特别是雌激素激素）的5'-调节核苷酸序列。 本发明还涉及用于各种目的的转基因鱼的方法，包括例如：（1）鉴定雌激素内分泌干扰物; （2）监测样品的雌激素样活性; （3）鉴定抗雌激素内分泌干扰物; 和（4）调查内分泌干扰物对肝脏再生的影响。 还公开了水生动物的表达盒，宿主细胞和转基因细胞。

    Promoter-regulated differentiation-dependent self-deleting cassette
    38.
    发明授权
    Promoter-regulated differentiation-dependent self-deleting cassette 有权
    启动子调节的分化依赖性自删除盒

    公开(公告)号：US08946505B2

    公开(公告)日：2015-02-03

    申请号：US13934829

    申请日：2013-07-03

    申请人： Regeneron Pharmaceuticals, Inc.

    发明人： David Frendewey ,  Guochun Gong ,  Ka-Man Venus Lai ,  David M. Valenzuela

    IPC分类号： C12N15/873 ,  A01K67/027 ,  C12N15/85 ,  C12N15/90

    CPC分类号： C12N15/85 ,  A01K67/0275 ,  A01K67/0276 ,  A01K2217/07 ,  A01K2217/075 ,  A01K2217/206 ,  A01K2227/105 ,  A01K2267/03 ,  C12N5/0606 ,  C12N9/1241 ,  C12N15/8509 ,  C12N15/873 ,  C12N15/907 ,  C12N2800/22 ,  C12N2800/30 ,  C12N2840/102 ,  C12Y207/07

    摘要： Targeting constructs and methods of using them are provided for differentiation-dependent modification of nucleic acid sequences in cells and in non-human animals. Targeting constructs comprising a promoter operably linked to a recombinase are provided, wherein the promoter drives transcription of the recombinase in an differentiated cell but not an undifferentiated cell. Promoters include Blimp1, Prm1, Gata6, Gata4, Igf2, Lhx2, Lhx5, and Pax3. Targeting constructs with a cassette flanked on both sides by recombinase sites can be removed using a recombinase gene operably linked to a 3′-UTR that comprises a recognition site for an miRNA that is transcribed in undifferentiated cells but not in differentiated cells. The constructs may be included in targeting vectors, and can be used to automatically modify or excise a selection cassette from an ES cell, a non-human embryo, or a non-human animal.

    摘要翻译： 提供靶向构建体和使用它们的方法用于细胞和非人动物中核酸序列的分化依赖性修饰。 提供了包含与重组酶可操作地连接的启动子的靶向构建体，其中所述启动子驱动分化细胞中但不是未分化细胞的重组酶的转录。 启动子包括Blimp1，Prm1，Gata6，Gata4，Igf2，Lhx2，Lhx5和Pax3。 可以使用可操作地连接到3'-UTR的重组酶基因来除去具有通过重组酶位点两侧的盒的靶向构建体，其包含在未分化细胞中但不在分化细胞中转录的miRNA的识别位点。 构建体可以包括在靶向载体中，并且可以用于自动修饰或从ES细胞，非人胚胎或非人动物中切除选择盒。

    TRANSGENIC CHICKENS WITH AN INACTIVATED ENDOGENOUS GENE LOCUS
    40.
    发明申请
    TRANSGENIC CHICKENS WITH AN INACTIVATED ENDOGENOUS GENE LOCUS 审中-公开
    具有灭活内源基因的转基因鸡肉

    公开(公告)号：US20140298504A1

    公开(公告)日：2014-10-02

    申请号：US14200401

    申请日：2014-03-07

    申请人： SYNAGEVA BIOPHARMA CORP.

    发明人： Marie-Cecile Van de Lavoir ,  Philip A. Leighton

    IPC分类号： A01K67/027

    CPC分类号： A01K67/0278 ,  A01K67/0271 ,  A01K67/0273 ,  A01K67/0275 ,  A01K67/0276 ,  A01K2207/15 ,  A01K2217/052 ,  A01K2217/072 ,  A01K2217/075 ,  A01K2217/206 ,  A01K2227/30 ,  A01K2267/01 ,  C07K14/43581 ,  C07K14/465 ,  C07K16/00 ,  C07K16/28 ,  C12N15/8509 ,  C12N2015/8518 ,  C12N2799/027 ,  C12N2800/30 ,  C12N2800/40

    摘要： The present invention is transgenic chickens obtained from long-term cultures of avian PGCs and techniques to produce and transgenic birds derived from prolonged PGC cultures. In some embodiments, these PGCs can be transfected with genetic constructs to modify the DNA of the PGC, specifically to introduce a transgene encoding an exogenous protein. When combined with a host avian embryo by known procedures, those modified PGCs are transmitted through the germline to yield transgenic offspring. This invention includes compositions comprising long-term cultures of PGCs and offspring derived from them that are genetically modified. The genetic modifications introduced into PGCs to achieve the gene inactivation may also include, but are not restricted to, random integrations of transgenes into the genome, transgenes inserted into the promoter region of genes, transgenes inserted into repetitive elements in the genome, site specific changes to the genome that are introduced using integrase, site specific changes to the genome introduced by homologous recombination, and conditional mutations introduced into the genome by excising DNA that is flanked by lox sites or other sequences that are substrates for site specific recombination.

    摘要翻译： 本发明是从禽类PGC的长期培养物获得的转基因鸡和产生延长的PGC培养物的转基因鸟的技术。 在一些实施方案中，这些PGC可以用遗传构建体转染以修饰PGC的DNA，特别是引入编码外源蛋白的转基因。 当通过已知方法与宿主禽类胚胎组合时，那些经修饰的PGC通过种系传播以产生转基因后代。 本发明包括包含PGC的长期培养物和从它们衍生的遗传修饰的后代的组合物。 导入PGC以实现基因失活的遗传修饰还可以包括但不限于转基因到基因组的随机整合，插入基因启动子区的转基因，插入基因组中的重复元件的转基因，位点特异性变化 使用整合酶引入的基因组，通过同源重组引入的基因组的位点特异性改变，以及通过切除侧翼为作为位点特异性重组的底物的lox位点或其他序列的DNA引入基因组的条件突变。