公开(公告)号：US20220186197A1

公开(公告)日：2022-06-16

申请号：US17558454

申请日：2021-12-21

申请人： Codexis, Inc.

发明人： Mathew G. Miller ,  Chinping Chng ,  Oscar Alvizo ,  Melissa Ann Mayo ,  James Nicholas Riggins ,  Xiang Yi ,  Jonathan S. Penfield ,  Gjalt W. Huisman ,  Jared Davis ,  Yasushi Saotome

IPC分类号： C12N9/12 ,  C12P19/34

摘要： The present invention provides engineered RNA polymerase variants and compositions comprising these variants. The present invention further provides engineered T7 RNA polymerase variants and compositions comprising these variants. These variants have been evolved for selective incorporation of the m7G(5′)ppp(5′)m7G cap analog over GTP at the initiation of in vitro transcription. The present invention also provides methods for selective capping of RNA transcripts.

公开(公告)号：US20210222146A1

公开(公告)日：2021-07-22

申请号：US17220684

申请日：2021-04-01

申请人： Codexis, Inc.

发明人： Gjalt W. Huisman ,  Nicholas J. Agard ,  Benjamin Mijts ,  Jonathan Vroom ,  Xiyun Zhang

IPC分类号： C12N9/88

摘要： The present invention provides engineered phenylalanine ammonia-lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia-lyase (PAL) polypeptides.

公开(公告)号：US20210222145A1

公开(公告)日：2021-07-22

申请号：US17219494

申请日：2021-03-31

申请人： Codexis, Inc.

发明人： Chinping Chng ,  William Casey Hallows ,  Nicholas J. Agard ,  Oscar Alvizo ,  Nikki Dellas ,  Gjalt W. Huisman ,  John Joseph Nicols

IPC分类号： C12N9/88 ,  C12N15/00

摘要： The present invention provides engineered phenylalanine ammonia lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia lyase (PAL) polypeptides. In some embodiments, the engineered PAL polypeptides are optimized to provide enhanced catalytic activity, as well as reduced sensitivity to proteolysis and increased tolerance to storage at elevated temperatures. In some embodiments, the engineered PAL polypeptides contain fewer phenylalanine residues than wild-type PAL polypeptides. The present invention also provides methods for the use of the compositions comprising the engineered PAL polypeptides for therapeutic and industrial purposes.

公开(公告)号：US20210139861A1

公开(公告)日：2021-05-13

申请号：US17149463

申请日：2021-01-14

申请人： Codexis, Inc.

发明人： Jack Liang ,  Stephane J. Jenne ,  Emily Mundorff ,  Charlene Ching ,  John M. Gruber ,  Anke Krebber ,  Gjalt W. Huisman

IPC分类号： C12N9/04 ,  C12P7/22

摘要： The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.

公开(公告)号：US20210130855A1

公开(公告)日：2021-05-06

申请号：US17148834

申请日：2021-01-14

申请人： Codexis, Inc.

发明人： Gjalt W. Huisman ,  Joyce Liu ,  Nikki Dellas ,  Chinping Chng ,  William Casey Hallows

IPC分类号： C12P7/42 ,  C12P13/22 ,  C12N15/63 ,  A61K9/00 ,  A61K31/122

摘要： The present invention provides engineered tyrosine ammonia-lyase (TAL) polypeptides and compositions thereof. In some embodiments, the engineered TAL polypeptides have been optimized to provide enhanced catalytic activity and enhanced acid stability, while reducing sensitivity to proteolysis and increasing tolerance to acidic pH levels. The invention also provides methods for utilization of the compositions comprising the engineered TAL polypeptides for therapeutic and industrial purposes.

公开(公告)号：US10947572B2

公开(公告)日：2021-03-16

申请号：US17002671

申请日：2020-08-25

申请人： CODEXIS, INC.

发明人： Haibin Chen ,  Steven J. Collier ,  Jovana Nazor ,  Joly Sukumaran ,  Derek Smith ,  Jeffrey C. Moore ,  Gregory Hughes ,  Jacob Janey ,  Gjalt W. Huisman ,  Scott J. Novick ,  Nicholas J. Agard ,  Oscar Alvizo ,  Gregory A. Cope ,  Wan Lin Yeo ,  Stefanie Ng Minor

IPC分类号： C12N9/06 ,  C12P13/02 ,  C12P17/18 ,  C12P13/04 ,  C12P13/06 ,  C12P17/10 ,  C12P17/12 ,  C12P17/16 ,  C12P13/00

摘要： The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.

公开(公告)号：US10941391B2

公开(公告)日：2021-03-09

申请号：US16653061

申请日：2019-10-15

申请人： Codexis, Inc.

发明人： Gjalt W. Huisman ,  Nicholas J. Agard ,  Benjamin Mijts ,  Jonathan Vroom ,  Xiyun Zhang

IPC分类号： C12N9/88 ,  A61K38/00

摘要： The present invention provides engineered phenylalanine ammonia-lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia-lyase (PAL) polypeptides.

公开(公告)号：US20190323044A1

公开(公告)日：2019-10-24

申请号：US16457396

申请日：2019-06-28

申请人： Codexis, Inc.

发明人： Jack S Liang ,  Stephan Jenne ,  Emily Mundorff ,  Rama Voladri ,  James J. Lalonde ,  Gjalt W. Huisman

IPC分类号： C12P17/00 ,  C12N9/04 ,  C12P17/16

摘要： The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize chiral compounds.

公开(公告)号：US10273229B2

公开(公告)日：2019-04-30

申请号：US15504120

申请日：2015-08-27

申请人： Codexis, Inc.

发明人： Gjalt W. Huisman ,  Jed Lee Hubbs ,  Xiyun Zhang ,  Robert Osborne

IPC分类号： C07D405/04 ,  C12Q1/48 ,  C07D405/14 ,  C12P17/16 ,  A61K31/517 ,  C07D487/04 ,  C07D487/08

摘要： The present invention provides kinase inhibitor analogs with improved properties, such as improved efficacy, pharmacokinetics, safety, and specificity. In some embodiments, the present invention provides lapatinib analogs that provide therapeutic benefits.

公开(公告)号：US20190078124A1

公开(公告)日：2019-03-14

申请号：US16195480

申请日：2018-11-19

申请人： CODEXIS, INC.

发明人： Haibin Chen ,  Steven J. Collier ,  Jovana Nazor ,  Joly Sukumaran ,  Derek Smith ,  Jeffrey C. Moore ,  Gregory Hughes ,  Jacob Janey ,  Gjalt W. Huisman ,  Scott J. Novick ,  Nicholas J. Agard ,  Oscar Alvizo ,  Gregory A. Cope ,  Wan Lin Yeo ,  Stefanie Ng Minor

IPC分类号： C12P13/02 ,  C12P17/10 ,  C12N9/06 ,  C12P17/18 ,  C12P13/04 ,  C12P13/06 ,  C12P17/12 ,  C12P17/16 ,  C12P13/00

摘要： The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.