公开(公告)号：US20230257721A1

公开(公告)日：2023-08-17

申请号：US17932420

申请日：2022-09-15

申请人： Codexis, Inc.

发明人： Jack Liang ,  Stephane J. Jenne ,  Emily Mundorff ,  Charlene Ching ,  John M. Gruber ,  Anke Krebber ,  Gjalt W. Huisman

IPC分类号： C12N9/04 ,  C12P7/22

CPC分类号： C12N9/0006 ,  C12P7/22 ,  C12Y101/01184 ,  Y02E50/10 ,  C12Y101/01 ,  Y02P20/52

摘要： The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.

公开(公告)号：US11479756B2

公开(公告)日：2022-10-25

申请号：US17149463

申请日：2021-01-14

申请人： Codexis, Inc.

发明人： Jack Liang ,  Stephane J. Jenne ,  Emily Mundorff ,  Charlene Ching ,  John M. Gruber ,  Anke Krebber ,  Gjalt W. Huisman

IPC分类号： C12N9/04 ,  C12P7/22

摘要： The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.

公开(公告)号：US10927351B2

公开(公告)日：2021-02-23

申请号：US16253972

申请日：2019-01-22

申请人： Codexis, Inc.

发明人： Jack Liang ,  Stephane J. Jenne ,  Emily Mundorff ,  Charlene Ching ,  John M. Gruber ,  Anke Krebber ,  Gjalt W. Huisman

IPC分类号： C12N9/04 ,  C12P7/22

摘要： The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.

公开(公告)号：US20180371428A1

公开(公告)日：2018-12-27

申请号：US16126761

申请日：2018-09-10

申请人： Codexis, Inc.

发明人： Jack Liang ,  Stephane J. Jenne ,  Emily Mundorff ,  Charlene Ching ,  John M. Gruber ,  Anke Krebber ,  Gjalt W. Huisman

IPC分类号： C12N9/04 ,  C12P7/22

摘要： The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.

公开(公告)号：US09528131B2

公开(公告)日：2016-12-27

申请号：US14501416

申请日：2014-09-30

申请人： Codexis, Inc.

发明人： Jack Liang ,  Stephane J. Jenne ,  Emily Mundorff ,  Charlene Ching ,  John M. Gruber ,  Anke Krebber ,  Gjalt W. Huisman

IPC分类号： C12P7/22 ,  C12N9/04

CPC分类号： C12N9/0006 ,  C12P7/22 ,  C12Y101/01 ,  C12Y101/01184 ,  Y02E50/17 ,  Y02P20/52

摘要： The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.

摘要翻译： 本公开提供了与天然存在的野生型酮还原酶相比具有改进性质的工程化酮还原酶。 还提供了编码工程化酮还原酶的多核苷酸，能够表达工程化酮还原酶的宿主细胞，以及使用工程化酮还原酶合成各种手性化合物的方法。

公开(公告)号：US08852909B2

公开(公告)日：2014-10-07

申请号：US13970284

申请日：2013-08-19

申请人： Codexis, Inc.

发明人： Jack Liang ,  Stephane J. Jenne ,  Emily Mundorff ,  Charlene Ching ,  John M. Gruber ,  Anke Krebber ,  Gjalt W. Huisman

IPC分类号： C12N9/02 ,  C07K14/00 ,  C12N9/04 ,  C12P7/22 ,  C12Q1/26 ,  C12P21/00

CPC分类号： C12N9/0006 ,  C12P7/22 ,  C12Y101/01 ,  C12Y101/01184 ,  Y02E50/17 ,  Y02P20/52

摘要： The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.

摘要翻译： 本公开提供与天然存在的野生型酮还原酶相比具有改进性质的工程化酮还原酶。 还提供了编码工程化酮还原酶的多核苷酸，能够表达工程化酮还原酶的宿主细胞，以及使用工程化酮还原酶合成各种手性化合物的方法。

公开(公告)号：US11512332B2

公开(公告)日：2022-11-29

申请号：US16932084

申请日：2020-07-17

申请人： Codexis, Inc.

发明人： Christopher Savile ,  John M. Gruber ,  Emily Mundorff ,  Gjalt W. Huisman ,  Steven J. Collier

IPC分类号： C12P17/00 ,  C12N9/04 ,  C12P13/00

摘要： The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N-methyl-3-keto-3-(2-thienyl)-1-propanamine to (S)—N-methyl-3-hydroxy-3-(2-thienyl)-1-propanamine.

公开(公告)号：US20210139861A1

公开(公告)日：2021-05-13

申请号：US17149463

申请日：2021-01-14

申请人： Codexis, Inc.

发明人： Jack Liang ,  Stephane J. Jenne ,  Emily Mundorff ,  Charlene Ching ,  John M. Gruber ,  Anke Krebber ,  Gjalt W. Huisman

IPC分类号： C12N9/04 ,  C12P7/22

摘要： The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.

公开(公告)号：US09873863B2

公开(公告)日：2018-01-23

申请号：US15353165

申请日：2016-11-16

申请人： Codexis, Inc.

发明人： Jack Liang ,  Stephane J. Jenne ,  Emily Mundorff ,  Charlene Ching ,  John M. Gruber ,  Anke Krebber ,  Gjalt W. Huisman

IPC分类号： C07H21/00 ,  C12N1/21 ,  C12N1/19 ,  C12N15/53 ,  C12N9/04 ,  C12P7/22

CPC分类号： C12N9/0006 ,  C12P7/22 ,  C12Y101/01 ,  C12Y101/01184 ,  Y02E50/17 ,  Y02P20/52

摘要： The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.

公开(公告)号：US20160083759A1

公开(公告)日：2016-03-24

申请号：US14954564

申请日：2015-11-30

申请人： CODEXIS, INC.

发明人： Christopher Savile ,  John M. Gruber ,  Emily Mundorff ,  Gjalt W. Huisman ,  Steven J. Collier

IPC分类号： C12P17/00 ,  C12N9/04

CPC分类号： C12P17/00 ,  C12N9/0006 ,  C12P13/001 ,  C12Y101/01164 ,  C12Y101/01184

摘要： The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N-methyl-3-keto-3-(2-thienyl)-1-propanamine to (S)—N-methyl-3-hydroxy-3-(2-thienyl)-1-propanamine.

摘要翻译： 本公开提供与天然存在的野生型酮还原酶相比具有改进性质的工程化酮还原酶。 还提供了编码工程化酮还原酶的多核苷酸，能够表达工程化酮还原酶的宿主细胞，以及使用工程化酮还原酶合成各种手性化合物的方法。 优化工程化的酮还原酶多肽，用于催化N-甲基-3-酮-3-（2-噻吩基）-1-丙胺向（S）-N-甲基-3-羟基-3-（2-噻吩基） -1-丙胺。