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    Polynucleotides encoding Δ-endotoxins toxic to lepidoptera and coleoptera, and method of use
    31.
    发明授权
    Polynucleotides encoding Δ-endotoxins toxic to lepidoptera and coleoptera, and method of use 有权
    编码对鳞翅目和鞘翅目有毒的δ-内毒素的多核苷酸及其使用方法

    公开(公告)号：US07022897B2

    公开(公告)日：2006-04-04

    申请号：US10120255

    申请日：2002-04-10

    申请人： Anne-Marie Light Mettus ,  James A. Baum

    发明人： Anne-Marie Light Mettus ,  James A. Baum

    IPC分类号： A01H5/00 ,  A01H1/00 ,  C12N15/32 ,  C12N1/21 ,  C12N15/82

    CPC分类号： C07K14/325 ,  C07K2319/00 ,  C12N15/8286 ,  Y02A40/162 ,  Y10S530/825

    摘要： Disclosed are novel polynucleotides encoding Lepidopteran- and Coleopteran-active δ-endotoxin polypeptides and compositions comprising the polypeptides. Also disclosed are vectors, transformed host cells, and transgenic plants that comprise the novel nucleic acid segments encoding the δ-endotoxin polypeptides. Also disclosed are methods of identifying related polynucleotides encoding δ-endotoxin polypeptides, methods of making and using transgenic cells comprising the novel sequences of the invention, as well as methods for controlling an insect population, such as the Western Corn Rootworm and Colorado potato beetle, and for conferring to a plant population resistance to the target insect species.

    摘要翻译： 公开了编码鳞翅目昆虫和鞘翅目昆虫活性δ-内毒素多肽的新型多核苷酸和包含该多肽的组合物。 还公开了载体，转化的宿主细胞和包含编码δ-内毒素多肽的新型核酸片段的转基因植物。 还公开了鉴定编码δ-内毒素多肽的相关多核苷酸的方法，制备和使用包含本发明的新序列的转基因细胞的方法，以及用于控制昆虫种群的方法，例如西部玉米根虫和科罗拉多马铃薯甲虫， 并赋予植物群体对目标昆虫物种的抗性。

    Nuclei acid and polypeptide compositions encoding lepidopteran-toxic polypeptides
    33.
    发明授权
    Nuclei acid and polypeptide compositions encoding lepidopteran-toxic polypeptides 有权
    编码鳞翅目昆虫毒性多肽的核酸和多肽组合物

    公开(公告)号：US06423828B1

    公开(公告)日：2002-07-23

    申请号：US09337280

    申请日：1999-06-22

    申请人： James A. Baum ,  Amy Jelen Gilmer ,  Anne-Marie Light Mettus

    发明人： James A. Baum ,  Amy Jelen Gilmer ,  Anne-Marie Light Mettus

    IPC分类号： C07K1400

    CPC分类号： C12R1/075 ,  C07K14/325 ,  C12N15/8286 ,  Y02A40/162

    摘要： Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding &dgr;-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the &agr; helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.

    摘要翻译： 公开了编码对鳞翅目昆虫具有杀虫活性的δ-内毒素的新型合成修饰的苏云金芽孢杆菌核酸片段。 还公开了由这些新型核酸序列编码的合成晶体蛋白质。 公开了制备和使用这些基因和蛋白质的方法以及重组表达和合适宿主细胞转化的方法。 转化的宿主细胞和表达修饰的内毒素的转基因植物也是本发明的方面。 还公开了用于修饰，改变和诱变这些晶体蛋白（包括Cry1C）的结构域1中的α螺旋之间的特异性环区的方法，以产生遗传工程改造的重组cry *基因及其编码的具有改善的杀虫活性的蛋白质。 在优选的实施方案中，公开了新的Cry1C *氨基酸片段和编码它们的经修饰的cry1C *核酸序列。

    Polypeptide compositions toxic to lepidopteran insects and methods for making same
    34.
    发明授权
    Polypeptide compositions toxic to lepidopteran insects and methods for making same 有权
    对鳞翅目昆虫有毒的多肽组合物及其制备方法

    公开(公告)号：US6153814A

    公开(公告)日：2000-11-28

    申请号：US250848

    申请日：1999-02-17

    申请人： James A. Baum ,  Amy Jelen Gilmer ,  Anne-Marie Light Mettus

    发明人： James A. Baum ,  Amy Jelen Gilmer ,  Anne-Marie Light Mettus

    IPC分类号： A01H5/00 ,  A01N63/00 ,  A01N63/02 ,  C07K14/325 ,  C07K16/12 ,  C12N1/21 ,  C12N15/09 ,  C12N15/31 ,  C12N15/82 ,  C12P21/02 ,  C12P21/08 ,  C12R1/38 ,  A01H1/04 ,  C12P21/06 ,  C07H17/00 ,  C07K14/00

    CPC分类号： C12R1/075 ,  C07K14/325 ,  C12N15/8286

    摘要： Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding .delta.-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the .alpha. helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.

    摘要翻译： 公开了编码对鳞翅目昆虫具有杀虫活性的δ-内毒素的新型合成修饰的苏云金芽孢杆菌核酸片段。 还公开了由这些新型核酸序列编码的合成晶体蛋白质。 公开了制备和使用这些基因和蛋白质的方法以及重组表达和合适宿主细胞转化的方法。 转化的宿主细胞和表达修饰的内毒素的转基因植物也是本发明的方面。 还公开了用于修饰，改变和诱变这些晶体蛋白（包括Cry1C）的结构域1中的α螺旋之间的特异性环区的方法，以产生遗传工程改造的重组cry *基因及其编码的具有改善的杀虫活性的蛋白质。 在优选的实施方案中，公开了新的Cry1C *氨基酸片段和编码它们的经修饰的cry1C *核酸序列。

    METHODS AND COMPOSITIONS FOR MAKING AND USING COMPATIBLE INSECTICIDAL PROTEINS
    36.
    发明申请
    METHODS AND COMPOSITIONS FOR MAKING AND USING COMPATIBLE INSECTICIDAL PROTEINS 审中-公开

    公开(公告)号：US20190276840A1

    公开(公告)日：2019-09-12

    申请号：US16296886

    申请日：2019-03-08

    申请人： James A. BAUM ,  Artem G. EVDOKIMOV ,  Agoston JERGA ,  Farhad MOSHIRI

    发明人： James A. BAUM ,  Artem G. EVDOKIMOV ,  Agoston JERGA ,  Farhad MOSHIRI

    IPC分类号： C12N15/82 ,  G01N33/50

    摘要： Pesticidal proteins (FFPP's) are used to produce derivatives (DP's) that are ineffective and disabled relative to conferring toxic properties upon a target pest, yet the ability of the DP to bind to the receptor to which said FFPP binds is unaffected. Such DP's are useful in inhibiting the FFPP from which it was derived when both are fed to a target pest and for comparing receptor binding capability and efficiency relative to different FFPP's from which the DP has been derived, providing for an assessment of different FFPP's relative to each other, and providing uniformity and certainty in combinations of such FFPP's for compositions, including transgenic plants, that can be used to control pest populations susceptible to both FFPP's, creating more durable transgenic plant products, inhibiting the development of resistance to such FFPP's when used in plants commercially, and in providing a durable and viable resistance management strategy for crops using such FFPP combinations. Polynucleotide sequences intended for use in expression of the DP's and FFPP's are also provided. Particular embodiments provide methods of designing and preparing DP's, as well as compositions and methods of using DP's and the FFPP's from which the DP's have been derived in more effective pesticidal compositions and products.