公开(公告)号：US08041515B2

公开(公告)日：2011-10-18

申请号：US11903454

申请日：2007-09-20

Applicant: Xiaobo Wang ,  Yama A. Abassi ,  Wen fu Zhang ,  Xiao Xu

Inventor： Xiaobo Wang ,  Yama A. Abassi ,  Wen fu Zhang ,  Xiao Xu

IPC: G06F19/00 ,  C12Q1/02 ,  C12M1/42

CPC classification number: G01N33/502

Abstract: The present invention provides methods of multi-dimensional profiling of biologically active agents and determining their effects on biological systems. The methods of the present invention include real-time impedance monitoring of cellular responses to biologically active agents and categorization of cellular kinetic profiles into mechanism specific cellular response profile groups. The grouping of similar cellular response profiles allows the correlation between agent and mechanism, thus allowing for the identification of potential therapeutic applications of agents or further study of cellular responses or mechanisms.

Abstract translation: 本发明提供生物活性剂的多维轮廓化方法，并确定其对生物系统的影响。 本发明的方法包括对生物活性剂的细胞反应的实时阻抗监测和细胞动力学特征分类到机制特异性细胞应答谱组中。 类似的细胞反应谱的分组允许试剂和机制之间的相关性，从而允许鉴定试剂的潜在治疗应用或进一步研究细胞应答或机制。

公开(公告)号：US07732127B2

公开(公告)日：2010-06-08

申请号：US11235938

申请日：2005-09-27

Applicant: Xiaobo Wang ,  Yama Abassi ,  Josephine Atienza ,  Xiao Xu

Inventor： Xiaobo Wang ,  Yama Abassi ,  Josephine Atienza ,  Xiao Xu

IPC: C12Q1/00 ,  C12N5/00

CPC classification number: G01N29/036 ,  G01N2291/0257

Abstract: The present invention includes devices and methods for dynamically monitoring cell adhesion and cell spreading. Cells are added to a microelectronic cell sensor array operably connected to an impedance analyzer. The device also includes a coating including biological molecule or organic compound capable of interacting with the cell. Cell adhesion and cell mobility is determined by detecting changes in impedance and comparing impedance or cell index values between samples.

Abstract translation: 本发明包括用于动态监测细胞粘附和细胞扩散的装置和方法。 将细胞添加到可操作地连接到阻抗分析器的微电子单元传感器阵列。 该装置还包括包含能够与细胞相互作用的生物分子或有机化合物的涂层。 通过检测阻抗变化并比较样品之间的阻抗或细胞指数值来确定细胞粘附和细胞迁移率。

公开(公告)号：US10690677B2

公开(公告)日：2020-06-23

申请号：US15487349

申请日：2017-04-13

Applicant: ACEA Biosciences, Inc.

Inventor： Xiao Xu ,  Yama A. Abassi ,  Xiaobo Wang ,  Jiangbo Gan

IPC: G01N33/68 ,  C12M1/34 ,  C12M1/12 ,  G01N33/50 ,  G01N33/483

Abstract: Methods for identifying compounds that modulate cellular responses stimulated by IgE, which include providing an impedance-based system that monitors cell-substrate impedance of cells on a substrate; introducing cells to the substrate of the system; adding at least one test compound and IgE to the cells, wherein the at least one test compound is suspected of modulating cell responses stimulated by the IgE; adding an antigen to the cells; monitoring the cell-substrate impedance of cells on the substrate; and analyzing the cell-substrate impedance to evaluate whether the at least one test compound alters a cellular response to stimulation with the IgE.

公开(公告)号：US10539523B2

公开(公告)日：2020-01-21

申请号：US14092927

申请日：2013-11-28

Applicant: ACEA Biosciences, Inc.

Inventor： Xiaobo Wang ,  Wei Ouyang ,  Nan Li ,  Tianxing Wang ,  Xiaoyu Zhang ,  Xiao Xu ,  Yama A. Abassi

IPC: G01N27/02

Abstract: Devices, systems and methods for monitoring excitable cells, such as cardiomyocytes, on microelectrode arrays that couple the electro-stimulation of excitable cells to induce or regulate cardiomyocyte beating and the simultaneous measurement of impedance and extracellular recording to assess changes in cardiomyocyte beating, viability, morphology or electrophysical properties in response to a plurality of treatments.

公开(公告)号：US10533985B2

公开(公告)日：2020-01-14

申请号：US16020679

申请日：2018-06-27

Applicant: ACEA Biosciences, Inc.

Inventor： Xiaobo Wang ,  Yama A. Abassi ,  Biao Xi ,  Wen Fu Zhang ,  Xiao Xu

IPC: G01N33/483 ,  G01N33/487

Abstract: A system for monitoring cell-substrate impedance of excitable cells and monitoring the beating cycle of cardiomyocytes, the system including: a device for monitoring cell-substrate impedance at 20 millisecond time resolution, the device having one or more wells on a nonconductive substrate, wherein at least one well of the one or more wells comprises an electrode array fabricated on the substrate for measurement of cell-substrate impedance at 20 millisecond time resolution; an impedance analyzer that measures cell-substrate impedance from the at least one well at 20 millisecond time resolution; electronic circuitry that electrically connects the electrode array from the at least one well to the impedance analyzer; and a software program that analyzes the measured cell-substrate impedance.

公开(公告)号：US10215748B2

公开(公告)日：2019-02-26

申请号：US13403976

申请日：2012-02-23

Applicant: Yama A. Abassi ,  Li Zhao ,  Ning Ke ,  Xiaobo Wang ,  Xiao Xu

Inventor： Yama A. Abassi ,  Li Zhao ,  Ning Ke ,  Xiaobo Wang ,  Xiao Xu

IPC: C40B30/04 ,  G01N33/50 ,  C12Q1/00 ,  G01N33/543

Abstract: Use of impedance devices in methods of generating a time dependent cellular profiles (TCRP) for the modulation of oncogene addicted cells and comparing the impedance-based TCRP to controls or knowns to identify signature time dependent cellular profiles.

公开(公告)号：US09869628B2

公开(公告)日：2018-01-16

申请号：US14750613

申请日：2015-06-25

Applicant: ACEA Biosciences, Inc.

Inventor： Nan Li ,  Sean Lee ,  Kilo Vilayphone ,  Xiaobo Wang

IPC: G01N15/14 ,  G01N15/10

CPC classification number: G01N15/1404 ,  G01N2015/1006 ,  G01N2015/1486

Abstract: A method of collecting cells from individual wells of a multi-well plate for use in flow cytometry, the method including adding a suspension of cells to wells of the multi-well plate; and aspirating cells from different wells according to a collection pattern into a flow cytometer, wherein the collection pattern is a sequential ordering of wells beginning at a middle region of the multi-well plate and continuing towards an outer region of the multi-well plate. The method preferably including rotating or agitating the multi-well plate between steps of aspirating cells from different wells. Exemplary collection patterns include spiral-square collection pattern and a nearest well to center collection pattern.

公开(公告)号：US09575063B2

公开(公告)日：2017-02-21

申请号：US14547963

申请日：2014-11-19

Applicant: ACEA Biosciences, Inc.

Inventor： Nan Li ,  Ye Chen ,  Hyunsun Chung ,  Zengqiang Li ,  Xiaobo Wang

IPC: G01N21/00 ,  G01N33/569 ,  G01N15/14 ,  G01N15/10

CPC classification number: G01N21/645 ,  G01N15/1434 ,  G01N15/1459 ,  G01N21/6486 ,  G01N33/56972 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1438 ,  G01N2015/1477 ,  G01N2021/6471 ,  G01N2021/6478 ,  G01N2201/06113

Abstract: An optical engine for use in a bench top flow cytometer, the optical engine comprising a set of lasers; a different set of beam shaping optics for each laser, wherein each set comprises two lenses to adjustably focus light horizontally along an x-axis to a same horizontal position and vertically along a y-axis to a different vertical position along a same plane; collection optics for collecting fluorescence from the flow cell; filtration optics that filter the collected fluorescence from the flow cell into different detection channels according to wavelength ranges; and a detector for each detection channel that converts the filtered fluorescence to electrical signals, wherein electrical signals are processed so that the fluorescence from each laser at the different vertical positions is distinguished at the same detector.

Abstract translation: 一种用于台式流式细胞仪的光学引擎，该光学引擎包括一组激光器; 用于每个激光器的不同组的光束整形光学器件，其中每个组件包括两个透镜，用于可调节地将光沿x轴水平地聚焦到相同的水平位置，并且沿着y轴沿着同一平面垂直地垂直于不同的垂直位置; 用于从流动池收集荧光的收集光学器件; 过滤光学器件，根据波长范围将从流通池收集的荧光过滤到不同的检测通道; 以及用于将滤波后的荧光转换为电信号的每个检测通道的检测器，其中对电信号进行处理，使得来自不同垂直位置处的每个激光的荧光在相同的检测器处被区分。

公开(公告)号：US09464089B2

公开(公告)日：2016-10-11

申请号：US14329890

申请日：2014-07-11

Applicant: ACEA BIOSCIENCES INC.

Inventor： Xiao Xu ,  Xiaobo Wang ,  Long Mao ,  Li Zhao ,  Biao Xi

IPC: A61K31/506 ,  C07D241/04 ,  C07D239/30 ,  C07D487/04 ,  C07D239/52 ,  C07D239/49 ,  C07D403/12 ,  C07D401/12 ,  C07D473/18 ,  C07D417/12

CPC classification number: C07D519/00 ,  C07D239/47 ,  C07D239/48 ,  C07D239/49 ,  C07D239/52 ,  C07D401/12 ,  C07D403/12 ,  C07D417/12 ,  C07D473/18 ,  C07D487/04

Abstract: The present invention relates to pharmaceutical compounds, compositions and methods, especially as they are related to compositions and methods for the treatment and/or prevention of a proliferation disorder, a cancer, a tumor, an inflammatory disease, an autoimmune disease, psoriasis, dry eye or an immunologically related disease, and in some embodiments diseases or disorders related to the dysregulation of kinase such as, but not limited to, EGFR (including HER), Alk, PDGFR, BLK, BMX/ETK, FLT3(D835Y), ITK, TEC, TXK, BTK, or JAK, and the respective pathways.

Abstract translation: 本发明涉及药物化合物，组合物和方法，特别是因为它们与用于治疗和/或预防增殖病症，癌症，肿瘤，炎性疾病，自身免疫性疾病，牛皮癣，干性疾病的组合物和方法有关 眼睛或免疫相关疾病，在一些实施方案中，与激酶失调相关的疾病或病症，例如但不限于EGFR（包括HER），Alk，PDGFR，BLK，BMX / ETK，FLT3（D835Y），ITK ，TEC，TXK，BTK或JAK，以及各自的途径。

公开(公告)号：US09200246B2

公开(公告)日：2015-12-01

申请号：US13309209

申请日：2011-12-01

Applicant: Donald Thomas ,  Wei Li ,  Ning Ke ,  Nan Li ,  Adrian Papas ,  Manfred Watzele ,  Alexander Seiler ,  Tianxing Wang ,  Yama Abassi ,  Xiaobo Wang

Inventor： Donald Thomas ,  Wei Li ,  Ning Ke ,  Nan Li ,  Adrian Papas ,  Manfred Watzele ,  Alexander Seiler ,  Tianxing Wang ,  Yama Abassi ,  Xiaobo Wang

IPC: C12M1/12 ,  C12Q1/02 ,  G01N33/543 ,  G01N21/25 ,  G01N21/64 ,  G01N33/487

CPC classification number: C12M25/04 ,  C12Q1/02 ,  G01N21/253 ,  G01N21/6452 ,  G01N33/48735 ,  G01N33/54373

Abstract: An insert device and co-culture device assembly incorporating the insert device, which includes an insert chamber with two fluid impermeable side walls that extend from a microporous bottom to an open top to form an inner cavity. The first side wall is shaped to form a convex arc that follows between about half of a circumference of the well and less than an entire circumference of the well. The second side wall joins ends of the first side wall and protrudes inward towards a center of the convex arc. A flange extends outward from or beneath the top and is notched to form a gap adjacent to the second side wall, which forms an access port allowing access to the lower chamber with a micropipette tip when the insert device is inserted into a well of a single or multi-well plate.

Abstract translation: 包括插入装置的插入装置和共培养装置组件，其包括具有从微孔底部延伸到开口顶部以形成内腔的两个流体不可渗透侧壁的插入室。 第一侧壁成形为形成沿着井的圆周的大约一半且小于井的整个圆周的凸弧。 第二侧壁连接第一侧壁的端部并朝向凸弧的中心向内突出。 凸缘从顶部向下或从顶部向外延伸并且被切口以形成与第二侧壁相邻的间隙，当形成插入装置插入到单个孔中时，形成允许通过微量移液管尖端进入下腔室的进入口 或多孔板。