公开(公告)号：US20160266116A1

公开(公告)日：2016-09-15

申请号：US14978413

申请日：2015-12-22

Applicant: Virginia Tech Intellectual Properties

Inventor： Xiang-Jin Meng ,  Yaowei Huang

IPC: G01N33/569 ,  C12N7/00 ,  A61K39/12

CPC classification number: G01N33/56983 ,  A61K39/12 ,  A61K2039/53 ,  A61K2039/552 ,  C07K14/005 ,  C12N7/00 ,  C12N15/86 ,  C12N2750/00021 ,  C12N2750/00022 ,  C12N2750/00034 ,  C12N2750/00043 ,  C12Q1/701 ,  G01N2333/01 ,  G01N2469/20

Abstract: The present invention also provides infectious DNA clones, biologically functional plasmid or viral vector containing the infectious nucleic acid genome molecule of Torque teno sus virus (TTsuV). The present invention also provides methods for diagnosing TTsuV infection via immunological methods, e.g., enzyme-linked immunoabsorbent assay (ELISA) and Western blot using PTTV specific antigens for detecting serum PTTV specific antibodies which indicate infections TTsuV1, TTsuV2, and individual TTsuV1 genotypes.

公开(公告)号：US09249192B2

公开(公告)日：2016-02-02

申请号：US13840805

申请日：2013-03-15

Applicant: Virginia Tech Intellectual Properties, Inc.

Inventor： Xiang-Jin Meng ,  Yaowei Huang

IPC: C07K14/005 ,  C12N7/00 ,  C12N15/86 ,  A61K39/12 ,  G01N33/569 ,  C12Q1/70 ,  A61K39/00

CPC classification number: G01N33/56983 ,  A61K39/12 ,  A61K2039/53 ,  A61K2039/552 ,  C07K14/005 ,  C12N7/00 ,  C12N15/86 ,  C12N2750/00021 ,  C12N2750/00022 ,  C12N2750/00034 ,  C12N2750/00043 ,  C12Q1/701 ,  G01N2333/01 ,  G01N2469/20

Abstract: The present invention also provides infectious DNA clones, biologically functional plasmid or viral vector containing the infectious nucleic acid genome molecule of Torque teno sus virus (TTsuV). The present invention also provides methods for diagnosing TTsuV infection via immunological methods, e.g., enzyme-linked immunoabsorbent assay (ELISA) and Western blot using PTTV specific antigens for detecting serum PTTV specific antibodies which indicate infections TTsuV1, TTsuV2, and individual TTsuV1 genotypes.

Abstract translation: 本发明还提供感染性DNA克隆，含有Torque teno sus病毒（TTsuV）的感染性核酸基因组分子的生物功能质粒或病毒载体。 本发明还提供了通过免疫学方法例如酶联免疫吸附测定（ELISA）和使用PTTV特异性抗原检测表明感染TTsuV1，TTsuV2和个体TTsuV1基因型的血清PTTV特异性抗体的蛋白质印迹来诊断TTsuV感染的方法。

公开(公告)号：US20150247165A1

公开(公告)日：2015-09-03

申请号：US14479803

申请日：2014-09-08

Applicant: DEUTSCHES KREBSFORSCHUNGSZENTRUM

Inventor： Harald zur HAUSEN ,  Ethel-Michele de VILLIERS

IPC: C12N15/86 ,  C12N7/00

CPC classification number: C12N15/86 ,  C07K14/005 ,  C12N7/00 ,  C12N2750/00021 ,  C12N2750/00022 ,  C12N2750/00034 ,  C12N2750/00043 ,  C12Q1/701

Abstract: Described are single-stranded new sequences of TT viruses, rearranged TTV sequences and hybrid molecules of a specific TT virus sequence and host cell DNA that are capable of replicating autonomously for use in diagnosis, prevention and treatment of diseases like cancer and autoimmunity. In addition, it relates to the use of such molecules as gene vectors and artificial chromosomes.

Abstract translation: 描述了TT病毒的单链新序列，重排的TTV序列和特异性TT病毒序列的混合分子以及能够自主复制用于诊断，预防和治疗诸如癌症和自身免疫的疾病的宿主细胞DNA。 此外，它涉及使用这样的分子作为基因载体和人造染色体。

公开(公告)号：US20150093403A1

公开(公告)日：2015-04-02

申请号：US14390668

申请日：2013-04-03

Applicant: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

Inventor： W. Ian Lipkin ,  Amit Kapoor ,  Edward J. Dubovi

IPC: C12N7/00 ,  C12Q1/70 ,  C07K14/005 ,  C07K16/08

CPC classification number: C12N7/00 ,  C07K14/005 ,  C07K16/081 ,  C12N2750/00021 ,  C12N2750/00033 ,  C12N2750/00071 ,  C12N2750/10021 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12Q1/701 ,  G01N33/56983 ,  G01N2469/20

Abstract: The invention is directed to a isolated a canine circoviruses associated with canine respiratory and gastrointestinal disease, and isolated nucleic acids sequences and polypeptides thereof. The invention also relates to antibodies against antigens from canine circoviruses. The invention also relates to iRNAs which target nucleic acid sequences of the canine circovirus. The invention is related to methods for detecting the presence or absence of canine circoviruses in an animal. The invention is also related to immunogenic compositions for inducing an immune response against canine circoviruses in an animal.

Abstract translation: 本发明涉及与犬呼吸道和胃肠道疾病相关的孤立的犬圆环病毒，以及分离的核酸序列及其多肽。 本发明还涉及针对来自犬圆环病毒抗原的抗体。 本发明还涉及靶向犬圆环病毒核酸序列的iRNA。 本发明涉及用于检测动物中犬圆环病毒存在或不存在的方法。 本发明还涉及用于诱导动物中犬圆环病毒的免疫应答的免疫原性组合物。

公开(公告)号：US20140286980A1

公开(公告)日：2014-09-25

申请号：US14122423

申请日：2012-05-24

Applicant: Jacqueline Gayle Marx

Inventor： Jacqueline Gayle Marx

IPC: C12N7/00

CPC classification number: C12N7/00 ,  A61K35/13 ,  A61K39/12 ,  A61K2039/5254 ,  A61K2039/552 ,  A61K2039/55566 ,  C12N7/02 ,  C12N15/86 ,  C12N2750/00021 ,  C12N2750/00034

Abstract: The present invention is directed to novel nucleotide and amino acid sequences of Torque teno virus (“TTV”), including novel genotypes thereof, all of which are useful in the preparation of vaccines for treating and preventing diseases in swine and other animals. Vaccines provided according to the practice of the invention are effective against multiple swine TTV genotypes and isolates. Diagnostic and therapeutic polyclonal and monoclonal antibodies are also a feature of the present invention, as are infectious clones useful in the propagation of the virus and in the preparation of vaccines. Particularly important aspects of the invention include polynucleotide constructs that replicate in tissue culture and in host swine. The invention also provides for novel full length TTV genomes that can replicate efficiently in host animals and tissue culture.

Abstract translation: 本发明涉及扭矩腱病毒（“TTV”）的新型核苷酸和氨基酸序列，其包括其新型基因型，所有这些都可用于制备用于治疗和预防猪和其他动物疾病的疫苗。 根据本发明的实践提供的疫苗对多种猪TTV基因型和分离株有效。 诊断和治疗性多克隆和单克隆抗体也是本发明的特征，以及用于病毒繁殖和疫苗制备中的感染性克隆也是如此。 本发明的特别重要的方面包括在组织培养和宿主猪中复制的多核苷酸构建体。 本发明还提供了可以在宿主动物和组织培养中有效复制的新型全长TTV基因组。

公开(公告)号：US20120321654A1

公开(公告)日：2012-12-20

申请号：US13501371

申请日：2010-04-16

Applicant: Gregory Paul Nitzel ,  Robert Gerard Ankenbauer ,  Jay Gregory Calvert ,  Donna Steuerwald Dunyak ,  Jacqueline Gayle Marx ,  Nancee Lois Oien ,  Douglas Steven Pearce ,  Mira Ivanova Stoeva ,  James Richard Thompson

Inventor： Gregory Paul Nitzel ,  Robert Gerard Ankenbauer ,  Jay Gregory Calvert ,  Donna Steuerwald Dunyak ,  Jacqueline Gayle Marx ,  Nancee Lois Oien ,  Douglas Steven Pearce ,  Mira Ivanova Stoeva ,  James Richard Thompson

IPC: A61K39/12 ,  C12N15/63 ,  C12N1/21 ,  C07K16/08 ,  C12N5/10 ,  C12N7/01 ,  C12N7/04 ,  C07K14/01 ,  C12N15/34 ,  C12N1/19

CPC classification number: A61K39/12 ,  A61K2039/552 ,  C07K14/005 ,  C07K16/081 ,  C07K2317/33 ,  C12N7/00 ,  C12N2750/00021 ,  C12N2750/00022 ,  C12N2750/00034 ,  C12N2750/10021 ,  C12N2750/10034

Abstract: The present invention is directed to novel nucleotide and amino acid sequences of Torque teno virus (“TTV”), including novel genotypes thereof, all of which are useful in the preparation of vaccines for treating and preventing diseases in swine and other animals. Vaccines provided according to the practice of the invention are effective against multiple swine TTV genotypes and isolates. Diagnostic and therapeutic polyclonal and monoclonal antibodies are also a feature of the present invention, as are infectious clones useful in the propagation of the virus and in the preparation of vaccines. Particularly important aspects of the invention include vaccines that provide TTV ORF1 protein, or peptide fragments thereof, as antigen.

Abstract translation: 本发明涉及扭矩腱病毒（TTV）的新型核苷酸和氨基酸序列，其包括其新型基因型，所有这些都可用于制备用于治疗和预防猪和其他动物疾病的疫苗。 根据本发明的实践提供的疫苗对多种猪TTV基因型和分离株有效。 诊断和治疗性多克隆和单克隆抗体也是本发明的特征，以及用于病毒繁殖和疫苗制备中的感染性克隆也是如此。 本发明的特别重要的方面包括提供TTV ORF1蛋白或其肽片段作为抗原的疫苗。

公开(公告)号：US20050221490A1

公开(公告)日：2005-10-06

申请号：US10819098

申请日：2004-04-05

Applicant: Thomas Tuschl ,  Sebastien Pfeffer

Inventor： Thomas Tuschl ,  Sebastien Pfeffer

IPC: C07H21/04 ,  C12N7/00 ,  C12N15/86 ,  C12Q1/68 ,  C12Q1/70

CPC classification number: C12N15/86 ,  C12N7/00 ,  C12N2750/00021 ,  C12N2750/00043

Abstract: The invention relates to isolated nucleic acid molecules comprising the sequence of a DNA virus microRNA. In another embodiment, the invention relates to single stranded DNA virus microRNA molecules. In yet another embodiment, the invention relates to single stranded anti-DNA virus microRNA molecules.

Abstract translation: 本发明涉及包含DNA病毒微小RNA序列的分离的核酸分子。 在另一个实施方案中，本发明涉及单链DNA病毒微RNA分子。 在另一个实施方案中，本发明涉及单链抗DNA病毒微小RNA分子。