公开(公告)号：US20180085735A1

公开(公告)日：2018-03-29

申请号：US15714368

申请日：2017-09-25

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： Jiali Liao ,  Christopher Belisle

IPC: B01J20/24 ,  C07K1/22 ,  C07K1/36 ,  C07K14/435 ,  C07K16/00 ,  B01J20/04 ,  B01J20/28 ,  B01J20/285 ,  B01D15/38

Abstract: Polymer-filled ceramic apatites and their uses are provided.

公开(公告)号：US09164058B2

公开(公告)日：2015-10-20

申请号：US14203351

申请日：2014-03-10

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Lei Li ,  Xuemei Yang ,  Christopher Belisle

IPC: G01N27/447 ,  C08F2/46 ,  C07K1/26 ,  B65D85/00 ,  B01D57/02 ,  C08K3/00

CPC classification number: G01N27/44747 ,  B01D57/02 ,  B65D85/70 ,  C07K1/26 ,  C08F2/46 ,  C08K3/00 ,  G01N21/6428

Abstract: Hand cast gels, and solutions for their preparation, are described.

Abstract translation: 描述了手铸凝胶及其制备方法。

公开(公告)号：US20140042027A1

公开(公告)日：2014-02-13

申请号：US13961626

申请日：2013-08-07

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Christopher Belisle

IPC: G01N27/447

CPC classification number: G01N27/44747 ,  G01N27/44713 ,  G01N27/44721 ,  G01N27/44726 ,  G01N27/44739

Abstract: Proteins that are electrophoretically separated in a gel are derivatized to produce fluorescent emissions by incorporating halo-substituted organic compounds into one or both of the electrode buffer solutions at the two ends of the gel. The halo-substituted compounds used are ones that bear an electric charge at the pH of the buffer solutions and gel, and the polarity of the charge on the compounds is such that the compounds migrate from the electrode buffer into the gel under the electrophoretic influence concurrently with the migration of the proteins into the gel. Once the proteins are separated and distributed within the gel and the gel is fully penetrated with the halo-substituted compounds, the gel is irradiated with ultraviolet light to induce a reaction between the halo-substituted compounds and the proteins through the tryptophan residues on the proteins, producing fluorescent reaction products.

Abstract translation: 在凝胶中电泳分离的蛋白质通过将卤素取代的有机化合物引入凝胶两端的一个或两个电极缓冲溶液中衍生而产生荧光发射。 使用的卤素取代的化合物是在缓冲溶液和凝胶的pH下具有电荷的化合物，并且化合物上的电荷的极性使得化合物在电泳影响下从电极缓冲液迁移到凝胶中 随着蛋白质迁移到凝胶中。 一旦蛋白质被分离并分布在凝胶中并且凝胶被卤素取代的化合物完全渗透，则用紫外线照射凝胶以通过蛋白质上的色氨酸残基诱导卤素取代的化合物与蛋白质之间的反应 ，产生荧光反应产物。

公开(公告)号：US20130306854A1

公开(公告)日：2013-11-21

申请号：US13896572

申请日：2013-05-17

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Christopher Belisle

IPC: G01N27/447 ,  G01N30/06 ,  H01J49/00

CPC classification number: G01N27/44721 ,  G01N30/06 ,  G01N33/6848 ,  H01J49/0036 ,  H01J49/26

Abstract: Disclosed herein is a method of preparing a protein sample for mass spectroscopy. The method includes separating proteins of the sample on an electrophoresis gel; contacting the proteins with a halo-substituted organic compound; exposing the gel to UV light; detecting fluorescence emitted from the electrophoresis gel; excising at least one portion of the electrophoresis gel based upon the detected fluorescence, wherein said at least one portion contains proteins of the protein sample; and subjecting proteins from the at least one portion to mass spectroscopy. Using this method, more proteins can be identified by GeLC-MS than when the electrophoresis gel is treated with a protein stain or subjected to the gel handling steps accompanying such treatment.

Abstract translation: 本文公开了一种制备用于质谱法的蛋白质样品的方法。 该方法包括在电泳凝胶上分离样品的蛋白质; 使蛋白质与卤素取代的有机化合物接触; 将凝胶暴露于紫外光下; 检测从电泳凝胶发出的荧光; 基于检测到的荧光来切割至少一部分电泳凝胶，其中所述至少一部分含有蛋白质样品的蛋白质; 并将来自至少一部分的蛋白质进行质谱分析。 使用这种方法，可以通过GeLC-MS鉴定更多的蛋白质，而不是用蛋白质染色剂处理电泳凝胶，或者进行伴随这种处理的凝胶处理步骤。