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21.发明申请STERILIZATION INDICATOR INCLUDING A SIMPLIFIED GENETICALLY ENGINEERED BIOLOGICAL INDICATOR 有权灭菌指示剂，包括简化的遗传工程生物指示剂公开(公告)号：US20140273072A1
公开(公告)日：2014-09-18
申请号：US13832019
申请日：2013-03-15
Applicant: AMERICAN STERILIZER COMPANY
Inventor： Phillip P. Franciskovich , Tricia A. Cregger , William A. Yirava
IPC: C12Q1/22
Abstract: A sterilization indicator, including a first compartment containing a genetically engineered biological indicator, and a second compartment containing an enzyme substrate, the second compartment adapted to maintain the enzyme substrate separate from the biological indicator during sterilization, and to permit the enzyme substrate to contact the biological indicator after the biological indicator has been exposed to the sterilization medium; in which the genetically engineered biological indicator comprises at least one test organism and at least one reporter gene suitable for producing an indicator enzyme, the reporter gene being taken up by the test organism; and the test organism is free of any active or activatable repressor gene that would inhibit expression of the reporter gene if present in the test organism or sterilization indicator, and the indicator enzyme and the enzyme substrate are selected such that enzymatic action of the indicator enzyme upon the enzyme substrate yields a detectable signal.
Abstract translation: 灭菌指示剂，包括含有遗传工程生物指示剂的第一隔室和含有酶底物的第二隔室，所述第二隔室适于在灭菌期间将酶底物与生物指示剂分离，并允许酶底物接触 生物指示剂在生物指示剂暴露于灭菌介质之后; 其中遗传工程生物指示剂包含至少一种测试生物体和至少一种适于产生指示酶的报告基因，所述报道基因被测试生物体吸收; 并且测试生物体不含任何活性或可激活的阻遏物基因，如果存在于测试生物或灭菌指示剂中，则抑制报告基因的表达，并且指示酶和酶底物被选择为使指示酶的酶作用 酶底物产生可检测信号。
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公开(公告)号：US10301632B2
公开(公告)日：2019-05-28
申请号：US16001266
申请日：2018-06-06
Applicant: American Sterilizer Company
Inventor： Phillip P. Franciskovich , Tricia A. Cregger , William A. Yirava , Peter A. Burke
IPC: C12Q1/22 , C12Q1/34 , C12Q1/689 , C12Q1/6897 , C12Q1/68 , C12N15/75 , A61L2/28 , C12M1/34 , C12M1/12 , C12N9/38
Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.
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公开(公告)号：US20180273958A1
公开(公告)日：2018-09-27
申请号：US16001266
申请日：2018-06-06
Applicant: American Sterilizer Company
Inventor： Phillip P. Franciskovich , Tricia A. Cregger , William A. Yirava , Peter A. Burke
IPC: C12N15/75 , C12Q1/689 , C12M1/34 , C12Q1/34 , C12N9/38 , C12Q1/6897 , C12M1/12 , C12Q1/22 , A61L2/28
CPC classification number: C12N15/75 , A61L2/28 , C12M1/34 , C12M37/06 , C12N9/2471 , C12Q1/22 , C12Q1/34 , C12Q1/689 , C12Q1/6897 , C12Y302/01023
Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.
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公开(公告)号：US10011844B2
公开(公告)日：2018-07-03
申请号：US15618157
申请日：2017-06-09
Applicant: American Sterilizer Company
Inventor： Phillip P. Franciskovich , Tricia A. Cregger , William A. Yirava , Peter A. Burke
IPC: C12Q1/22 , C12Q1/34 , C12Q1/68 , C12N15/75 , C12M1/12 , C12N9/38 , A61L2/28 , C12M1/34 , C12Q1/6897 , C12Q1/689
Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.
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Patent Agency Ranking
公开(公告)号：US20170275633A1
公开(公告)日：2017-09-28
申请号：US15618158
申请日：2017-06-09
Applicant: American Sterilizer Company
Inventor： Phillip P. Franciskovich , Tricia A. Cregger , William A. Yirava , Peter A. Burke
CPC classification number: C12N15/75 , A61L2/28 , C12M1/34 , C12M37/06 , C12N9/2471 , C12Q1/22 , C12Q1/34 , C12Q1/689 , C12Q1/6897 , C12Y302/01023
Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.
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公开(公告)号：US20160312232A1
公开(公告)日：2016-10-27
申请号：US15204079
申请日：2016-07-07
Applicant: American Sterilizer Company
Inventor： Phillip P. Franciskovich , Tricia A. Cregger , William A. Yirava , Peter A. Burke
CPC classification number: C12N15/75 , A61L2/28 , C12M1/34 , C12M37/06 , C12N9/2471 , C12Q1/22 , C12Q1/34 , C12Q1/689 , C12Q1/6897 , C12Y302/01023
Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.
Abstract translation: 本发明涉及一种衍生自组合物的生物指示剂，其包含：宿主生物，其包含孢子形成细菌; 用于产生指示酶的报告基因; 调节基因; 以及用于将报道基因和调节基因插入宿主生物体的载体; 宿主生物体在其基因组中具有转座遗传元件，用于将插入序列插入调节基因; 所述插入序列包含转座酶，一对末端反向重复序列和用于表达转座酶的至少一个开放阅读框。 载体可能被宿主生物体吸收。 插入序列可插入调节基因。 宿主生物体可能经历孢子形成以形成生物指示剂。 公开了一种使用生物指示剂的方法和装置。
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公开(公告)号：US09416393B2
公开(公告)日：2016-08-16
申请号：US14512708
申请日：2014-10-13
Applicant: American Sterilizer Company
Inventor： Phillip P. Franciskovich , Tricia A. Cregger , William A. Yirava , Peter A. Burke
CPC classification number: C12N15/75 , A61L2/28 , C12M1/34 , C12M37/06 , C12N9/2471 , C12Q1/22 , C12Q1/34 , C12Q1/689 , C12Q1/6897 , C12Y302/01023
Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.
Abstract translation: 本发明涉及一种衍生自组合物的生物指示剂，其包含：宿主生物，其包含孢子形成细菌; 用于产生指示酶的报告基因; 调节基因; 以及用于将报道基因和调节基因插入宿主生物体的载体; 宿主生物体在其基因组中具有转座遗传元件，用于将插入序列插入到调节基因中; 所述插入序列包含转座酶，一对末端反向重复序列和用于表达转座酶的至少一个开放阅读框。 载体可能被宿主生物体吸收。 插入序列可插入调节基因。 宿主生物体可能经历孢子形成以形成生物指示剂。 公开了一种使用生物指示剂的方法和装置。
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28.发明授权公开(公告)号：US08858884B2
公开(公告)日：2014-10-14
申请号：US13832158
申请日：2013-03-15
Applicant: American Sterilizer Company
Inventor： Phillip P. Franciskovich , Tricia A. Cregger
IPC: G01N27/00 , C12Q1/22 , C12Q1/00 , G01N27/327
CPC classification number: C12Q1/22 , C12Q1/001 , C12Q1/006 , G01N27/327 , G01N33/52 , G01N2400/00 , Y10T436/11 , Y10T436/25375
Abstract: A sterilization indicator system and method of using the system to determine efficacy of a sterilization process. The system includes a vial having a first compartment containing spores of one or more species of microorganism; a second compartment containing a growth medium with a disaccharide, an oligosaccharide or a polysaccharide in which the vial is free of monosaccharide; an enzyme, capable of acting upon the monosaccharide to yield reaction products and electron transfer, disposed on two or more electrodes adapted to carry an electrical signal resulting from the electron transfer, the pair of electrodes positioned to contact the combined contents of the first compartment and the second compartment during incubation; and an apparatus linked or linkable to the electrodes and adapted to detect and measure the electrical signal resulting from electron transfer when the enzyme acts upon the monosaccharide.
Abstract translation: 灭菌指示器系统和使用该系统来确定灭菌过程的功效的方法。 该系统包括具有包含一种或多种微生物孢子的第一隔室的小瓶; 第二隔室含有具有二糖的生长培养基，寡糖或多糖，其中小瓶不含单糖; 能够作用于单糖以产生反应产物和电子转移的酶，其设置在适于承载由电子转移产生的电信号的两个或多个电极上，该对电极定位成接触第一隔室的组合内容物，以及 孵化期间的第二隔室; 以及连接或可链接到电极的装置，并且适于在酶作用于单糖时检测和测量由电子转移产生的电信号。
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公开(公告)号：US20140273073A1
公开(公告)日：2014-09-18
申请号：US13840509
申请日：2013-03-15
Applicant: AMERICAN STERILIZER COMPANY
Inventor： Phillip P. Franciskovich , Tricia A. Cregger
IPC: C12Q1/22
Abstract: A sterilization indicator for oxidative sterilants, comprising a first compartment comprising spores of one or more microorganism species, wherein the spores have been pretreated with and comprises a compound comprising a transition metal ion that is reactive with an oxidative sterilant; a second compartment comprising a growth medium and adapted to combine contents of the first compartment with contents of the second compartment for incubation after the sterilization indicator has been exposed to an oxidative sterilant; and an agent disposed in the growth medium and selected to indicate viability of the spores after the sterilization indicator has been exposed to the oxidative sterilant.
Abstract translation: 一种用于氧化灭菌剂的消毒指示剂，其包含由一种或多种微生物种类的孢子组成的第一隔室，其中所述孢子已经用包含与氧化灭菌剂反应的过渡金属离子预处理并包含其中的化合物; 第二隔室，包括生长介质，并且适于将所述第一隔室的内容物与所述第二隔室的内容物组合，以在所述灭菌指示剂暴露于氧化灭菌剂之后进行孵育; 以及设置在生长培养基中并选择用于指示灭菌指示剂暴露于氧化灭菌剂之后的孢子的活力的试剂。
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