公开(公告)号：US11660961B2

公开(公告)日：2023-05-30

申请号：US17067610

申请日：2020-10-09

申请人： The Regents of the University of California

发明人： Taek Soon Lee ,  Aram Kang

IPC分类号： C12N9/88 ,  B60K35/00 ,  C07F9/09 ,  C12P5/00 ,  C12N9/16 ,  C12P7/04 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C08F136/08 ,  B65G69/00 ,  G01S13/931

CPC分类号： B60K35/00 ,  B65G69/006 ,  C07F9/091 ,  C07F9/098 ,  C08F136/08 ,  C12N9/0006 ,  C12N9/1025 ,  C12N9/1205 ,  C12N9/16 ,  C12N9/88 ,  C12P5/007 ,  C12P7/04 ,  C12Y101/01088 ,  C12Y203/0301 ,  C12Y207/01036 ,  C12Y401/01033 ,  B60K2370/173 ,  G01S2013/9314 ,  G01S2013/9317 ,  G01S2013/9323 ,  G01S2013/9324

摘要： The present invention provides for a genetically modified host cell capable of producing isopentenol and/or 3-methyl-3-butenol, comprising (a) an increased expression of phosphomevalonate decarboxylase (PMD) (b) an increased expression of a phosphatase capable of converting isopentenol into 3-methyl-3-butenol, (c) optionally the genetically modified host cell does not express, or has a decreased expression of one or more of NudB, phosphomevalonate kinase (PMK), and/or PMD, and (d) optionally one or more further enzymes capable of converting isopentenol and/or 3-methyl-3-butenol into a third compound, such as isoprene.

公开(公告)号：US11566265B2

公开(公告)日：2023-01-31

申请号：US17314561

申请日：2021-05-07

申请人： Massachusetts Institute of Technology ,  National University of Singapore

发明人： Parayil K. Ajikumar ,  Gregory Stephanopoulos ,  Heng Phon Too

IPC分类号： C12P5/00 ,  C12P17/02 ,  C12P23/00 ,  C12P15/00 ,  C12P7/42 ,  C12P19/56 ,  C12N15/70 ,  C12P9/00 ,  C12Q1/689

摘要： The invention relates to the production of one or more terpenoids through microbial engineering, and relates to the manufacture of products comprising terpenoids.

公开(公告)号：US11555212B2

公开(公告)日：2023-01-17

申请号：US17160149

申请日：2021-01-27

申请人： CysBio ApS

发明人： Christian Bille Jendresen ,  Alex Toftgaard Nielsen

IPC分类号： C12P11/00 ,  C12N9/10 ,  C12P5/00

摘要： The present invention generally relates to the field of biotechnology as it applies to the production of aryl sulfates using recombinant host cells. More particularly, the present invention pertains to recombinant host cells comprising (e.g., expressing) a polypeptide having aryl sulfotransferase activity, wherein said recombinant host cells have been modified to have an increased uptake of sulfate compared to identical host cells that does not carry said modification. Further provided are processes for the production of aryl sulfates, such as zosteric acid, employing such recombinant host cells.

公开(公告)号：US20220396802A1

公开(公告)日：2022-12-15

申请号：US17774705

申请日：2020-11-05

申请人： Sichuan Honghe Biotechnology Co., Ltd.

发明人： Luqi Huang ,  Ping Su ,  Rui Ma ,  Guanghong Cui ,  Juan Guo ,  Baolong Jin ,  Yating Hu ,  Jichen Bao

IPC分类号： C12N15/81 ,  C12N9/88 ,  C12N15/82 ,  C12N15/70 ,  C12P5/00 ,  C12P7/02

摘要： Provided are a Cinnamomum burmannii monoterpene synthase CbTPS1, an amino acid sequence thereof, a nucleic acid molecule encoding the protein, an use thereof in preparing the monoterpene synthase, and a method for preparing dextrorotatory borneol by using the Cinnamomum burmannii monoterpene synthase CbTPS1.

公开(公告)号：US20220325313A1

公开(公告)日：2022-10-13

申请号：US17712452

申请日：2022-04-04

申请人： Conagen Inc.

发明人： Yisheng Wu ,  Wanli Lu ,  Jacob Thomas Courtney ,  David Nunn ,  Oliver Yu

IPC分类号： C12P23/00 ,  C12P5/00 ,  C12N1/16 ,  C12N15/81 ,  C12N15/62 ,  C12N15/52

摘要： Provided herein are recombinant nucleic acid molecules, nucleic acid constructs, fusion enzymes, transformed host cells, and methods for making aroma compounds alpha-ionone or beta-ionone.

公开(公告)号：US20220315911A1

公开(公告)日：2022-10-06

申请号：US17837269

申请日：2022-06-10

申请人： ISOBIONICS B.V.

发明人： Martinus Julius BEEKWILDER ,  Adèle Margaretha Maria Liduina Van Houwelingen ,  Hendrik Jan Bosch ,  Georg Friedrich Lentzen ,  Elena Melillo ,  Hendrik Wouter Wisselink

IPC分类号： C12N9/88 ,  C07K16/40 ,  C12P5/00 ,  C12P7/02 ,  C12N15/80 ,  C12N15/82

摘要： The invention is directed to a santalene synthase, to a nucleic acid encoding said santalene synthase, to an expression vector comprising said nucleic acid, to a host cell comprising said expression vector, to a method of preparing santalene, to a method of preparing santalol and to a method of preparing a santalene synthase. The invention is further directed to an antibody specific for the santalane synthase.

公开(公告)号：US11453884B2

公开(公告)日：2022-09-27

申请号：US16990802

申请日：2020-08-11

申请人： University of Kentucky Research Foundation

发明人： Joe Chappell ,  Xun Zhuang ,  Shuiqin Wu

IPC分类号： C12N15/81 ,  C12N15/01 ,  C12P5/00

摘要： A method is provided for producing modified mutant yeast and the resulting yeast that can be used as a platform for terpene production. The method includes chemical mutagenesis to effect ergosterol dependent growth in yeast. Subsequently, these yeast are subjected to an erg9 knockout mutation to thereby produce ergosterol dependent growth/erg9 knockout mutation yeast cell lines. The resulting yeast are well suited for use in the production of terpenes.

公开(公告)号：US11352648B2

公开(公告)日：2022-06-07

申请号：US16992773

申请日：2020-08-13

申请人： Manus Bio, Inc.

发明人： Ajikumar Parayil Kumaran ,  Ryan Lim ,  Jason Donald ,  Hsien-Chung Tseng ,  Christine Nicole S. Santos ,  Ryan Philippe

IPC分类号： C12N15/52 ,  C12P5/00 ,  C12N9/02 ,  C12P7/04

摘要： The invention relates to methods and bacterial strains for making terpene and terpenoid products, the bacterial strains having improved carbon pull through the MEP pathway and to a downstream recombinant synthesis pathway.

公开(公告)号：US20220056485A1

公开(公告)日：2022-02-24

申请号：US17463830

申请日：2021-09-01

申请人： Firmenich SA

发明人： Laurent Daviet ,  Letizia Rocci ,  Michel Schalk

IPC分类号： C12P5/00 ,  C12P7/02 ,  C12N9/02

摘要： This invention relates generally to methods and compositions for producing a sesquiterpene alcohol comprising contacting a sesquiterpene with a P450 polypeptide with monooxygenase activity.

公开(公告)号：US11236365B2

公开(公告)日：2022-02-01

申请号：US16502750

申请日：2019-07-03

申请人： SUMITOMO RUBBER INDUSTRIES, LTD. ,  TOHOKU University

发明人： Yukino Inoue ,  Haruhiko Yamaguchi ,  Kazuhisa Fushihara ,  Seiji Takahashi ,  Satoshi Yamashita ,  Toru Nakayama

IPC分类号： C12N15/82 ,  C12P5/00 ,  C12N9/10 ,  B29D30/08 ,  B29D30/06 ,  C07K14/415 ,  B29K607/00

摘要： Provided is a method for producing a polyisoprenoid, which can increase natural rubber production by enhancing the rubber synthesis activity of rubber particles. The present invention provides methods for producing a polyisoprenoid using a gene coding for a cis-prenyltransferase (CPT) family protein, a gene coding for a Nogo-B receptor (NgBR) family protein and a gene coding for a rubber elongation factor (REF) family protein, specifically a method for producing a polyisoprenoid in vitro using rubber particles bound to proteins coded for by these genes, and a method for producing a polyisoprenoid in vivo using a recombinant organism (plant) having these genes introduced therein.