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公开(公告)号：US11214775B2
公开(公告)日：2022-01-04
申请号：US15734887
申请日：2019-07-10
申请人： FIRMENICH SA
摘要： Provided herein are biocatalytic methods of producing terpene compounds by applying a novel type of phosphatase enzyme. The method allows the fully biochemical synthesis of terpene compounds, like for example copalol and labdendiol, and derivatives thereof, which serve as valuable intermediates for the production of perfumery ingredients, such as, for example, ambrox. Also provided are novel fully biochemical multistep processes for the production of such compounds as well as novel phosphatase enzymes and mutants and variants derived therefrom.
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公开(公告)号：US20210010035A1
公开(公告)日：2021-01-14
申请号：US16938605
申请日：2020-07-24
申请人： FIRMENICH SA
摘要： Described herein are methods of producing (+)-manool, the methods including: contacting geranylgeranyl diphosphate with a copalyl diphosphate (CPP) synthase to form a (9S, 10S)-copalyl diphosphate and contacting the CPP with a sclareol synthase enzyme to form (+)-manool and derivatives thereof. Also described herein are nucleic acids encoding CPP synthases and sclareol synthases for use in the methods. Further described herein are expression vectors and non-human host organisms and cells including nucleic acids encoding a CPP synthase and a sclareol synthase as described herein.
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公开(公告)号：US20200172936A1
公开(公告)日：2020-06-04
申请号：US16738563
申请日：2020-01-09
申请人： Firmenich SA
发明人： Laurent Daviet , Letizia Rocci , Michel Schalk
摘要： This invention relates generally to methods and compositions for producing a sesquiterpene alcohol comprising contacting a sesquiterpene with a P450 polypeptide with monooxygenase activity.
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公开(公告)号：US09267155B2
公开(公告)日：2016-02-23
申请号：US14136938
申请日：2013-12-20
申请人： FIRMENICH SA
发明人： Michel Schalk
摘要： The present invention provides a method of producing sclareol, the method comprising contacting a particular polypeptide having a sclareol synthase activity with labdenediol diphosphate (LPP). In particular, the method may be carried out in vitro or in vivo to produce sclareol, a very useful compound in the fields of perfumery and flavoring. The present invention also provides the amino acid sequence of the polypeptide used in the method. A nucleic acid derived from Salvia sclarea and encoding the polypeptide of the invention, an expression vector containing the nucleic acid, as well as a non-human organism or a cell transformed to harbor the same nucleic acid, are also part of the present invention.
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公开(公告)号：US11293040B2
公开(公告)日：2022-04-05
申请号：US16308890
申请日：2017-07-19
申请人： Firmenich SA
发明人： Michel Schalk , Letizia Rocci
摘要： Provided is a method of producing one or more sesquiterpene compounds comprising: contacting an acyclic FPP precursor with a polypeptide having terpene synthase activity, wherein the polypeptide comprises an amino acid sequence that has at least 55% sequence identity to SEQ ID NO: 1, to produce one or more terpenes selected from the group consisting of isovalencene, spirovetiva-1(10),7(11)-diene and valencene or derivatives thereof, or mixture of sesquiterpenes comprising one or more of isovalencene, spirovetiva-1(10),7(11)-diene and/or valencene; and optionally isolating the one or more terpenes or the mixture. Also described is a nucleic acid derived from Vetiveria zizanoides encoding a polypeptide having sesquiterpene synthase activity, a polypeptide that can be used to produce one or more sesquiterpenes or a mixture of sesquiterpenes comprising one or more of isovalencene, spirovetiva-1(10),7(11)-diene and/or valencene, and a non-human organism or cell comprising the nucleic acid or comprising an expression vector comprising the nucleic acid.
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公开(公告)号：US10385363B2
公开(公告)日：2019-08-20
申请号：US15330814
申请日：2015-05-06
申请人： Firmenich SA
摘要： The present invention relates to a method of producing drimenol and/or drimenol derivatives by contacting at least one polypeptide with farnesyl diphosphate. The method may be performed in vitro or in vivo. The present invention also provides amino acid sequences of polypeptides useful in the method of the invention and nucleic acid encoding the polypeptides of the invention. The method further provides host cells or organisms genetically modified to express the polypeptides of the invention and useful to produce drimenol and/or drimenol derivatives.
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公开(公告)号：US09969999B2
公开(公告)日：2018-05-15
申请号：US15060060
申请日：2016-03-03
申请人： FIRMENICH SA
发明人： Michel Schalk
CPC分类号： C12N9/88 , C12P5/007 , C12P15/00 , C12Y402/03082
摘要： The present invention provides a method of producing α-santalene by contacting at least one polypeptide with farnesyl phyrophosphate (fpp). In particular, the method may be carried out in vitro or in vivo to produce α-santalene, a very useful compound in the fields of perfumery and flavoring. The present invention also provides the amino acid sequence of a polypeptide useful in the method of the invention. A nucleic acid encoding the polypeptide of the invention and an expression vector containing the nucleic acid represent part of the present invention. A non-human host organism and a cell transformed to be used in the method of producing α santalene are also part of the present invention.
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公开(公告)号：US20230151393A1
公开(公告)日：2023-05-18
申请号：US16766225
申请日：2019-01-16
申请人： FIRMENICH SA
发明人： Michel Schalk , Letizia Rocci , Fabienne Deguerry
CPC分类号： C12P7/02 , C12N5/10 , C12N15/63 , C12N15/52 , C12N9/0077 , C12Y114/15006 , C12P5/007
摘要： Described herein are the nucleic acid and the amino acid sequences of a cytochrome P450 capable of oxidizing terpene molecules. Also described herein are methods of oxidizing terpene molecules including contacting the cytochrome P450 with the terpene molecule intended to be oxidized. In particular, the method may be carried out in vitro or in vivo to produce oxidized terpene molecules, which may be used in different technical fields such as for example perfumery and flavoring. Also described herein is an expression vector containing the nucleic acid. A non-human host organism or a cell transformed with the nucleic acid is also an object of the disclosure.
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公开(公告)号：US20230148463A9
公开(公告)日：2023-05-11
申请号：US17673465
申请日：2022-02-16
申请人： FIRMENICH SA
CPC分类号： C12P7/02 , C12N9/88 , C12Y402/03
摘要： Described herein is a method of producing a drimane sesquiterpene such as albicanol, drimenol and/or derivatives thereof by contacting at least one polypeptide with farnesyl diphosphate (FPP) with a polypeptide including a Haloacid dehalogenase (HAD)-like hydrolase domain and having bifunctional terpene synthase activity. The method may be performed in vitro or in vivo. Also described herein are amino acid sequences of polypeptides useful in the methods and nucleic acids encoding the polypeptides described. The described method further provides host cells or organisms genetically modified to express the polypeptides and useful to produce a drimane sesquiterpene such as albicanol, drimenol and/or derivatives thereof.
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公开(公告)号：US09777293B2
公开(公告)日：2017-10-03
申请号：US14530651
申请日：2014-10-31
申请人： FIRMENICH SA
发明人： Michel Schalk
CPC分类号： C12P5/002 , C12N9/88 , C12P5/007 , C12Y402/03083
摘要： The present invention provides a method of producing β-santalene, said method comprising contacting at least one polypeptide with farnesyl pyrophosphate (FPP). In particular, said method may be carried out in vitro or in vivo to produce β-santalene, a very useful compound in the fields of perfumery and flavoring. The present invention also provides the amino acid sequence of a polypeptide useful in the method of the invention. A nucleic acid encoding the polypeptide of the invention and an expression vector containing said nucleic acid are also part of the present invention. A non-human host organism or a cell transformed to be used in the method of producing β-santalene is also an object of the present invention.
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