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公开(公告)号：US11542508B2
公开(公告)日：2023-01-03
申请号：US16346124
申请日：2017-11-28
发明人： Rotem Sorek , Gil Amitai , Erez Zohar
摘要： Methods of expressing an expression product of interest are provided. Accordingly there is provided a method comprising introducing into a cell a polynucleotide comprising an AimR responsive element operatively linked to a nucleic acid sequence encoding the expression product of interest, and contacting said cell with an AimP peptide comprising an amino acid sequence of XXXXGG/A, wherein said AimP peptide is capable of binding said AimR polypeptide and dissociating said AimR polypeptide from said AimR responsive element. Also provided are articles of manufacture, isolated peptides, polynucleotides and nucleic acid constructs.
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公开(公告)号：US11447782B2
公开(公告)日：2022-09-20
申请号：US16081082
申请日：2017-03-06
申请人： DANISCO US INC.
发明人： Cristina Bongiorni , Marc Kolkman , Chris Leeflang , Virgil Arthur Rhodius , Anita Van Kimmenade
摘要： The instant disclosure is generally related to novel engineered (hybrid) promoters. In certain embodiments, the instant disclosure is directed to one or more nucleic acid compositions comprising engineered promoters operably linked to nucleic acids encoding proteins of interest. Thus, the disclosure set forth herein described methods and compositions for the production of proteins of interest using one or more novel engineered (hybrid) promoters of the disclosure.
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公开(公告)号：US20220282234A1
公开(公告)日：2022-09-08
申请号：US17635349
申请日：2020-08-10
申请人： DANISCO US INC.
发明人： Ryan L. FRISCH , Hongxian HE
摘要： The present disclosure is generally related to compositions and methods for constructing and obtaining Bacillus licheniformis cells having increased protein production phenotypes. Thus, certain embodiments are related to modified B. licheniformis cells derived from parental B. licheniformis cells. Certain embodiments are related to modified B. licheniformis cells comprising a modified rghR locus. Certain embodiments are related to modified B. licheniformis cells having a modified rghR locus and comprising an increased protein productivity phenotype. In certain other embodiments, modified B. licheniformis cells having a modified rghR locus produce a reduced amount of red pigment. In certain other embodiments, modified B. licheniformis cells comprise an increased protein productivity phenotype and produce a reduced amount of red pigment.
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14.发明授权公开(公告)号：US11414643B2
公开(公告)日：2022-08-16
申请号：US16957217
申请日：2018-12-12
申请人： DANISCO US INC.
发明人： Shannon Del Chase , Carol Marie Fioresi , Ryan L. Frisch , Helen Olivia Masson , Anita Van Kimmenade
摘要： The instant disclosure is generally related to novel Bacillus sp. mutants capable of producing increased amounts of industrially relevant proteins of interest. Certain embodiments of the disclosure are related to modified Bacillus sp. cells comprising an introduced polynucleotide encoding a variant GlcT protein. Other embodiments are related to methods and compositions for producing endogenous and/or heterologous proteins of interest in the modified Bacillus sp. (daughter) cells, whereas certain other embodiments are directed to nucleic acid sequences, particularly polynucleotide open reading frame (ORF) sequences, vectors thereof and DNA expression constructs thereof, encoding variant GlcT proteins of the disclosure.
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15.发明申请公开(公告)号：US20220186234A1
公开(公告)日：2022-06-16
申请号：US17432203
申请日：2020-02-18
申请人： BASF SE
发明人： Andreas Daub , Aydin Golabgir Anbarani , Tobias Klein , Stephan Freyer , Thomas Kaeding , Max Fabian Felle , Paul Igor Costea
摘要： The present invention is directed to an industrial fermentation process for cultivating a Bacillus cell in a chemically defined fermentation medium and a method for producing a protein of interest comprising the steps of providing a chemically defined fermentation medium, inoculating the fermentation medium with a Bacillus cell comprising a gene encoding a protein of interest, cultivating the Bacillus cell in the fermentation medium under conditions conductive for the growth of the Bacillus cell and the expression of the protein of interest, wherein the cultivation of the Bacillus cell comprises the addition of one or more feed solutions comprising one or more chemically defined carbon sources and one or more trace element ions to the fermentation medium.
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公开(公告)号：US20220186203A1
公开(公告)日：2022-06-16
申请号：US17442724
申请日：2020-03-23
申请人： METGEN OY
摘要： The present invention relates to endoglucanase variants with improved properties and uses thereof as eco-friendly biocatalysts in various industrial processes. More in particular the invention provides a polypeptide with endoglucanase activity (EC 3.2.1.4) comprising an amino acid sequence that is at least 90% identical to the amino acid sequence according to SEQ ID NO: 1 or SEQ ID NO: 2, wherein the polypeptide comprises an amino acid selected from the group consisting of Glycine, Threonine, Serine, Alanine, Leucine, Isoleucine and Valine at a position corresponding to position 579 in SEQ ID NO: 1 or SEQ ID NO: 2 or an amino acid selected from the group consisting of Serine, Alanine and Valine at a position corresponding to position 656 in SEQ ID NO: 1 or SEQ ID NO: 2.
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公开(公告)号：US11306319B2
公开(公告)日：2022-04-19
申请号：US15771618
申请日：2016-10-27
申请人： DANISCO US INC
摘要： The present disclosure is generally related to modified Gram positive bacterial cells producing increased amounts of one or more protein(s) of interest and modified Gram positive bacterial cells having increased genetic competency. Thus, certain embodiments of the disclosure are directed to modified Gram positive bacterial cells expressing an increased amount of a protein of interest, relative to an unmodified (parental) Gram positive bacterial cell expressing the same protein of interest, wherein the modified bacterial cell comprises at least one mutation in a rpoC gene encoding a variant RNA-polymerase (RNAP) β′-subunit polypeptide. In certain embodiments, the rpoC gene encoding the variant β′-subunit polypeptide is integrated into the chromosome of the modified cell. In other embodiments, the rpoC gene encoding the variant β′-subunit polypeptide is comprised on an extrachromosomal plasmid introduced into the modified cell. In other embodiments, the disclosure is directed to competent Bacillus host cells comprising at least one copy of a nucleic acid construct encoding a modified rpoC polypeptide comprising 90% sequence identity to SEQ ID NO: 8 and an aspartic acid to glycine substitution at position 796 of SEQ ID NO: 8, wherein the polynucleotide encoding the rpoC polypeptide is foreign to the Bacillus host cell that was non-competent prior to the introduction of the first nucleic acid construct.
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公开(公告)号：US20220024996A1
公开(公告)日：2022-01-27
申请号：US17499599
申请日：2021-10-12
IPC分类号： C07K14/37 , C07K7/64 , C12P21/02 , C12N9/10 , C12N9/02 , C12N15/70 , C12N15/75 , C12N15/77 , C12N15/78 , C12N15/81
摘要： An object of the present invention is to identify an enzyme having activity of synthesizing dihydrotentoxin that is a tentoxin precursor and an enzyme having activity of synthesizing tentoxin using dihydrotentoxin as a substrate. The present invention concerns a tentoxin synthesis-related gene encoding a protein comprising the amino acid sequence of SEQ ID NO: 16 and having activity of nonribosomal peptide synthesis of dihydrotentoxin and a tentoxin synthesis-related gene encoding a protein comprising the amino acid sequence of SEQ ID NO: 18 and having activity of converting dihydrotentoxin to tentoxin.
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公开(公告)号：US20210340512A1
公开(公告)日：2021-11-04
申请号：US17335188
申请日：2021-06-01
申请人： Novozymes A/S
发明人： Morten Gjermansen , Jesper Salomon , Klaus Gori
摘要： The present invention relates to polypeptides having DNase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
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公开(公告)号：US11130969B2
公开(公告)日：2021-09-28
申请号：US14897060
申请日：2014-07-04
申请人： Novozymes A/S
摘要： The present invention provides methods for directly transferring a recombinant polynucleotide of interest from the chromosome of a prokaryotic donor cell to the chromosome of a prokaryotic recipient cell of another species, wherein the polynucleotide of interest comprises at least one coding sequence of interest, preferably one gene of interest and a selectable marker, wherein said polynucleotide of interest in the chromosome of the donor cell is flanked on each side by a polynucleotide region derived from the chromosome of the recipient cell, and wherein the flanking polynucleotide regions are of sufficient size and sequence identity to allow double homologous recombination with the corresponding regions in the chromosome of the recipient cell.
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