公开(公告)号：US20180327757A1

公开(公告)日：2018-11-15

申请号：US15771618

申请日：2016-10-27

Applicant: DANISCO US INC

Inventor： Cristina Bongiorni ,  Marguerite A. CERVIN ,  George England ,  Chao Ahu ,  Frank Wouter Koopman

IPC: C12N15/75 ,  C12N9/12 ,  C12P21/02 ,  C12R1/01

CPC classification number: C12N15/75 ,  C12N9/1247 ,  C12N2320/50 ,  C12N2330/51 ,  C12N2800/101 ,  C12P21/00 ,  C12P21/02 ,  C12R1/01

Abstract: The present disclosure is generally related to modified Gram positive bacterial cells producing increased amounts of one or more protein(s) of interest and modified Gram positive bacterial cells having increased genetic competency. Thus, certain embodiments of the disclosure are directed to modified Gram positive bacterial cells expressing an increased amount of a protein of interest, relative to an unmodified (parental) Gram positive bacterial cell expressing the same protein of interest, wherein the modified bacterial cell comprises at least one mutation in a rpoC gene encoding a variant RNA-polymerase (RNAP) β′-subunit polypeptide. In certain embodiments, the rpoC gene encoding the variant β′-subunit polypeptide is integrated into the chromosome of the modified cell. In other embodiments, the rpoC gene encoding the variant β′-subunit polypeptide is comprised on an extrachromosomal plasmid introduced into the modified cell. In other embodiments, the disclosure is directed to competent Bacillus host cells comprising at least one copy of a nucleic acid construct encoding a modified rpoC polypeptide comprising 90% sequence identity to SEQ ID NO: 8 and an aspartic acid to glycine substitution at position 796 of SEQ ID NO: 8, wherein the polynucleotide encoding the rpoC polypeptide is foreign to the Bacillus host cell that was non-competent prior to the introduction of the first nucleic acid construct.

公开(公告)号：US20140295475A1

公开(公告)日：2014-10-02

申请号：US14355835

申请日：2012-12-04

Applicant: Danisco US Inc.

Inventor： George England ,  Suzanne Lantz

IPC: C12N9/24 ,  C12Q1/40 ,  C12Q1/34

CPC classification number: C12Q1/40 ,  C12N9/00 ,  C12P19/02 ,  C12P19/14 ,  C12P21/00 ,  C12P39/00 ,  C12Q1/34 ,  C12Y302/01 ,  Y02E50/16 ,  Y02E50/17

Abstract: The application provides methods of producing a mixture of enzymes using two or more cell lines, methods of identifying or constructing cell lines for producing a mixture of enzymes, and methods of preparing a cell bank for producing a mixture of enzymes.

Abstract translation: 本申请提供了使用两种或多种细胞系产生酶混合物的方法，鉴定或构建用于产生酶混合物的细胞系的方法，以及制备用于产生酶混合物的细胞库的方法。

公开(公告)号：US11306319B2

公开(公告)日：2022-04-19

申请号：US15771618

申请日：2016-10-27

Applicant: DANISCO US INC

Inventor： Cristina Bongiorni ,  Marguerite A. Cervin ,  George England ,  Chao Zhu ,  Frank Wouter Koopman

IPC: C12N15/00 ,  C12N15/75 ,  C12N9/12 ,  C12P21/02 ,  C12P21/00 ,  C12N1/20 ,  C12R1/01

Abstract: The present disclosure is generally related to modified Gram positive bacterial cells producing increased amounts of one or more protein(s) of interest and modified Gram positive bacterial cells having increased genetic competency. Thus, certain embodiments of the disclosure are directed to modified Gram positive bacterial cells expressing an increased amount of a protein of interest, relative to an unmodified (parental) Gram positive bacterial cell expressing the same protein of interest, wherein the modified bacterial cell comprises at least one mutation in a rpoC gene encoding a variant RNA-polymerase (RNAP) β′-subunit polypeptide. In certain embodiments, the rpoC gene encoding the variant β′-subunit polypeptide is integrated into the chromosome of the modified cell. In other embodiments, the rpoC gene encoding the variant β′-subunit polypeptide is comprised on an extrachromosomal plasmid introduced into the modified cell. In other embodiments, the disclosure is directed to competent Bacillus host cells comprising at least one copy of a nucleic acid construct encoding a modified rpoC polypeptide comprising 90% sequence identity to SEQ ID NO: 8 and an aspartic acid to glycine substitution at position 796 of SEQ ID NO: 8, wherein the polynucleotide encoding the rpoC polypeptide is foreign to the Bacillus host cell that was non-competent prior to the introduction of the first nucleic acid construct.

公开(公告)号：US20160264927A1

公开(公告)日：2016-09-15

申请号：US15031833

申请日：2014-10-27

Applicant: DANISCO US INC.

Inventor： Andrei Miasnikov ,  Douglas A. Dale ,  Douglas Ko ,  George England ,  Wyatt C. Smith ,  Beth Fryksdale ,  Matthew T. Reboli

IPC: C12N1/18 ,  C12N9/34 ,  C12P7/06

CPC classification number: C12N1/18 ,  C12N1/04 ,  C12N9/18 ,  C12N9/2402 ,  C12N9/242 ,  C12N9/2428 ,  C12N9/80 ,  C12P7/06 ,  C12P7/08 ,  C12Y302/01003 ,  Y02E50/17

Abstract: The present teachings provide large scale genetically engineered yeast in active dry form.

Abstract translation: 本教导提供主要干燥形式的大规模遗传工程酵母。