公开(公告)号：US20130259869A1

公开(公告)日：2013-10-03

申请号：US13719835

申请日：2012-12-19

Applicant: Deutsches Krebsforschungszentrum Stiftung Des Offentlichen Rechtes

Inventor： Ethel-Michele De Villiers ,  Harald Zur Hausen

IPC: C07K14/01 ,  C12N15/113 ,  C12Q1/70 ,  C07K16/08 ,  G01N33/569

CPC classification number: C07K14/01 ,  C07K14/005 ,  C07K16/081 ,  C12N7/00 ,  C12N15/1131 ,  C12N2750/00021 ,  C12N2750/00022 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q1/701 ,  G01N33/56983 ,  G01N2800/50

Abstract: The present invention relates to rearranged molecules of (a) a specific TT virus sequence and (b) a nucleotide sequence encoding a polypeptide showing homology to mammalian proteins associated with cancer and autoimmune diseases that are capable of replicating autonomously for use in diagnosis, prevention and treatment of diseases like cancer and autoimmunity.

Abstract translation: 本发明涉及（a）特定TT病毒序列的重排分子和（b）编码与癌症和自身免疫疾病相关的哺乳动物蛋白质具有同源性的多肽的核苷酸序列，其能够自主复制以用于诊断，预防和 治疗癌症和自身免疫等疾病。

公开(公告)号：US5756708A

公开(公告)日：1998-05-26

申请号：US202186

申请日：1994-02-24

Applicant: Mirko Karan ,  Thomas Michael Burns ,  James Langham Dale ,  Robert Maxwell Harding

Inventor： Mirko Karan ,  Thomas Michael Burns ,  James Langham Dale ,  Robert Maxwell Harding

IPC: C07K14/01 ,  C12N15/11 ,  C12N15/34

CPC classification number: C07K14/005 ,  C12N2750/00021 ,  C12N2750/00022

Abstract: The invention provides DNA molecules consisting essentially of a nucleotide sequence or part thereof which are asosciated with the genome of banana bunchy top virus (BBTV) as illustrated in FIGS. 1 through 8 (SEQ ID NOS: 49-54, 27-37, 55-60, 38-48, and 9-23, respectively) of the specification attached herewith.

Abstract translation: 本发明提供了基本上由与如图1和2所示的香蕉束顶病毒（BBTV）的基因组一致的核苷酸序列或其部分组成的DNA分子。 1至8（分别为SEQ ID NO：49-54,27-37,55-60,38-48和9-23）。

公开(公告)号：US20190211361A1

公开(公告)日：2019-07-11

申请号：US16366571

申请日：2019-03-27

Applicant: FLAGSHIP PIONEERING INNOVATIONS V, INC.

Inventor： Avak Kahvejian ,  Erica Gabrielle Weinstein ,  Nicholas McCartney Plugis ,  Kevin James Lebo ,  Fernando Martin Diaz ,  Dhananjay Maniklal Nawandar

IPC: C12N15/86 ,  C12N15/113 ,  C07K14/005

CPC classification number: C12N15/86 ,  C07K14/005 ,  C12N15/113 ,  C12N2310/141 ,  C12N2320/32 ,  C12N2750/00021 ,  C12N2750/00022 ,  C12N2750/00043

Abstract: This invention relates generally to pharmaceutical compositions and preparations of curons and uses thereof.

公开(公告)号：US09676828B2

公开(公告)日：2017-06-13

申请号：US13719835

申请日：2012-12-19

Applicant: Deutsches Krebsforschungszentrum

Inventor： Ethel-Michele De Villiers ,  Harald Zur Hausen

IPC: C07K14/01 ,  C12N7/00 ,  C07K16/08 ,  G01N33/569 ,  C12Q1/70 ,  C12N15/113 ,  C12Q1/68 ,  C07K14/005

CPC classification number: C07K14/01 ,  C07K14/005 ,  C07K16/081 ,  C12N7/00 ,  C12N15/1131 ,  C12N2750/00021 ,  C12N2750/00022 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q1/701 ,  G01N33/56983 ,  G01N2800/50

Abstract: The present invention relates to rearranged molecules of (a) a specific TT virus sequence and (b) a nucleotide sequence encoding a polypeptide showing homology to mammalian proteins associated with cancer and autoimmune diseases that are capable of replicating autonomously for use in diagnosis, prevention and treatment of diseases like cancer and autoimmunity.

公开(公告)号：US09228242B2

公开(公告)日：2016-01-05

申请号：US12861378

申请日：2010-08-23

Applicant: Xiang-Jin Meng ,  Yaowei Huang

Inventor： Xiang-Jin Meng ,  Yaowei Huang

IPC: C12N15/33 ,  A61K39/12 ,  C12N15/70 ,  C12N1/21 ,  C12N7/00 ,  C12Q1/70 ,  C07K14/005 ,  C07K16/08 ,  G01N33/569 ,  A61K39/00

CPC classification number: G01N33/56983 ,  A61K39/12 ,  A61K2039/552 ,  C07K14/005 ,  C07K16/081 ,  C12N7/00 ,  C12N2750/00021 ,  C12N2750/00022 ,  C12N2750/00034 ,  C12N2750/14034 ,  C12Q1/701 ,  G01N2333/01 ,  G01N2333/085 ,  G01N2469/20

Abstract: The present invention provides four purified preparation containing a polynucleic acid molecule encoding porcine Torque teno virus (PTTV) genotypes or subtypes PTTV1a-VA, PTTV1b-VA, PTTV2b-VA, and PTTV2c-VA. The present invention also provides infectious DNA clones, biologically functional plasmid or viral vector containing the infectious nucleic acid genome molecule of the same. The present invention further provides live, attenuated, vector-expressed and purified recombinant capsid subunit or killed viral vaccines for protection against PTTV infection. The present invention additionally provides subunit vaccines comprising PTTV specific gene products, especially ORF1 capsid gene product for protection against PTTV infection. Further, the present invention provides methods for diagnosing PTTV infection via polymerase chain reaction (PCR) using specific primer for PTTV1, PTTV2, and individual PTTV1 genotypes. Finally, the present invention provides methods for diagnosing PTTV infection via immunological methods, e.g., enzyme-linked immunoabsorbent assay (ELISA) and Western blot using PTTV specific antigens for detecting serum PTTV specific antibodies.

Abstract translation: 本发明提供了含有编码猪扭转腱病毒（PTTV）基因型或亚型PTTV1a-VA，PTTV1b-VA，PTTV2b-VA和PTTV2c-VA的多核酸分子的四种纯化制剂。 本发明还提供感染性DNA克隆，含有该感染性核酸基因组分子的生物功能质粒或病毒载体。 本发明进一步提供活的，减毒的载体表达和纯化的重组衣壳亚单位或灭活的病毒疫苗以防止PTTV感染。 本发明另外提供了包含PTTV特异性基因产物的亚单位疫苗，特别是用于保护免受PTTV感染的ORF1衣壳基因产物。 此外，本发明提供了使用PTTV1，PTTV2和个体PTTV1基因型的特异性引物通过聚合酶链反应（PCR）诊断PTTV感染的方法。 最后，本发明提供了通过免疫学方法诊断PTTV感染的方法，例如使用PTTV特异性抗原检测血清PTTV特异性抗体的酶联免疫吸附测定（ELISA）和蛋白质印迹。

公开(公告)号：US20130315944A1

公开(公告)日：2013-11-28

申请号：US13840805

申请日：2013-03-15

Applicant: Virginia Tech Intellectual Properties, Inc.

Inventor： Xiang-Jin MENG ,  Yaowei HUANG

IPC: C07K14/005 ,  C12N7/00 ,  G01N33/569 ,  C12N15/86

CPC classification number: G01N33/56983 ,  A61K39/12 ,  A61K2039/53 ,  A61K2039/552 ,  C07K14/005 ,  C12N7/00 ,  C12N15/86 ,  C12N2750/00021 ,  C12N2750/00022 ,  C12N2750/00034 ,  C12N2750/00043 ,  C12Q1/701 ,  G01N2333/01 ,  G01N2469/20

Abstract: The present invention also provides infectious DNA clones, biologically functional plasmid or viral vector containing the infectious nucleic acid genome molecule of Torque teno sus virus (TTsuV). The present invention also provides methods for diagnosing TTsuV infection via immunological methods, e.g., enzyme-linked immunoabsorbent assay (ELISA) and Western blot using PTTV specific antigens for detecting serum PTTV specific antibodies which indicate infections TTsuV1, TTsuV2, and individual TTsuV1 genotypes.

Abstract translation: 本发明还提供感染性DNA克隆，含有Torque teno sus病毒（TTsuV）的感染性核酸基因组分子的生物功能质粒或病毒载体。 本发明还提供了通过免疫学方法例如酶联免疫吸附测定（ELISA）和使用PTTV特异性抗原检测表明感染TTsuV1，TTsuV2和个体TTsuV1基因型的血清PTTV特异性抗体的蛋白质印迹来诊断TTsuV感染的方法。

公开(公告)号：US20120040438A1

公开(公告)日：2012-02-16

申请号：US13142090

申请日：2009-11-27

Applicant: Zoltan Penzes ,  Ferenc Misák ,  Tamás Tuboly ,  Attila Csagola

Inventor： Zoltan Penzes ,  Ferenc Misák ,  Tamás Tuboly ,  Attila Csagola

IPC: C12N7/01 ,  C12N7/02 ,  C12N5/071

CPC classification number: C12N7/00 ,  A61K2039/5254 ,  A61K2039/555 ,  C12N2750/00021 ,  C12N2750/10021

Abstract: The present invention relates to the novel Porcine Circovirus type 2 subtype B (PCV2B-Rm) isolate which comprises a short duplication of sequence and is adapted to grow in cell culture and may be propagated at high titres constantly up to 106 TCID50 viral particles per mL. This novel isolate is particularly useful for the production of porcine circovirus type 2 (PCV2) vaccines, for treating and/or preventing and/or diagnosing porcine circovirus associated diseases, as well as for diagnosing the presence of porcine circovirus in pigs. The present invention also relates to novel cell clones derived from Swine Testicles (ST) useful for propagating PCV2 and to a method for production of PCV2 virus with particularly high titers.

Abstract translation: 本发明涉及新型猪圆环病毒2型亚型B（PCV2B-Rm）分离物，其包含短的重复序列，并且适于在细胞培养物中生长并且可以以高滴度持续增殖至多达106TCID 50病毒颗粒/ mL 。 该新型分离物特别可用于生产猪圆环病毒2型（PCV2）疫苗，用于治疗和/或预防和/或诊断猪圆环病毒相关疾病，以及用于诊断猪圆环病毒在猪中的存在。 本发明还涉及可用于繁殖PCV2的猪睾丸（ST）的新型细胞克隆，以及特异性高滴度产生PCV2病毒的方法。

公开(公告)号：US20110318363A1

公开(公告)日：2011-12-29

申请号：US12821634

申请日：2010-06-23

Applicant: Harald zur Hausen ,  Ethel-Michele de Villiers

Inventor： Harald zur Hausen ,  Ethel-Michele de Villiers

IPC: A61K39/42 ,  C12N15/63 ,  C12N7/00 ,  C07K14/01 ,  G06F17/30 ,  C12Q1/70 ,  A61K31/713 ,  A61K39/12 ,  A61P31/12 ,  C07H21/04 ,  C07K16/08

CPC classification number: C12N15/86 ,  C07K14/005 ,  C12N7/00 ,  C12N2750/00021 ,  C12N2750/00022 ,  C12N2750/00034 ,  C12N2750/00043 ,  C12Q1/701

Abstract: Described are single-stranded new sequences of TT viruses, rearranged TTV sequences and hybrid molecules of a specific TT virus sequence and host cell DNA that are capable of replicating autonomously for use in diagnosis, prevention and treatment of diseases like cancer and autoimmunity. In addition, it relates to the use of such molecules as gene vectors and artificial chromosomes.

Abstract translation: 描述了TT病毒的单链新序列，重排的TTV序列和特异性TT病毒序列的混合分子以及能够自主复制用于诊断，预防和治疗诸如癌症和自身免疫的疾病的宿主细胞DNA。 此外，它涉及使用这样的分子作为基因载体和人造染色体。

公开(公告)号：US20050221293A1

公开(公告)日：2005-10-06

申请号：US10925363

申请日：2004-08-24

Applicant: Thomas Tuschl ,  Sebastien Pfeffer

Inventor： Thomas Tuschl ,  Sebastien Pfeffer

IPC: C07H21/04 ,  C12N7/00 ,  C12N15/86 ,  C12Q1/68 ,  C12Q1/70

CPC classification number: C12N15/86 ,  C12N7/00 ,  C12N2750/00021 ,  C12N2750/00043

Abstract: The invention relates to isolated nucleic acid molecules comprising the sequence of any one of the DNA virus microRNAs shown in Table A1. In another embodiment, the invention relates to single stranded DNA virus microRNA molecules and anti-DNA virus microRNA molecules.

Abstract translation: 本发明涉及分离的核酸分子，其包含表A1所示的任何一种DNA病毒微小RNA的序列。 在另一个实施方案中，本发明涉及单链DNA病毒微小RNA分子和抗DNA病毒微小RNA分子。

公开(公告)号：US09624511B2

公开(公告)日：2017-04-18

申请号：US14479803

申请日：2014-09-08

Applicant: Deutsches Krebsforschungszentrum

Inventor： Harald zur Hausen ,  Ethel-Michele de Villiers

IPC: C12N15/86 ,  C12N7/00 ,  C12Q1/70 ,  C07K14/005

CPC classification number: C12N15/86 ,  C07K14/005 ,  C12N7/00 ,  C12N2750/00021 ,  C12N2750/00022 ,  C12N2750/00034 ,  C12N2750/00043 ,  C12Q1/701

Abstract: Described are single-stranded new sequences of TT viruses, rearranged TTV sequences and hybrid molecules of a specific TT virus sequence and host cell DNA that are capable of replicating autonomously for use in diagnosis, prevention and treatment of diseases like cancer and autoimmunity. In addition, it relates to the use of such molecules as gene vectors and artificial chromosomes.